DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ yang-2011-7 in vitro In vitro ion exchange chrom ion exchange chromatography IEC IEC ion exchange chrom predetermined predetermined participant predetermined Yang Q., Coseno M., Gilmartin GM., Doublié S. DIP original experiment: DIP-218778X Structure (London, England : 1993) 2011 IMEx Crystal structure of a human cleavage factor CFI(m)25/CFI(m)68/RNA complex provides an insight into poly(A) site recognition and RNA looping. Yang Q., Coseno M., Gilmartin GM., Doublié S. Only protein-protein interactions imex curation yang-2011-8 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Yang Q., Coseno M., Gilmartin GM., Doublié S. DIP original experiment: DIP-218780X Structure (London, England : 1993) 2011 IMEx Crystal structure of a human cleavage factor CFI(m)25/CFI(m)68/RNA complex provides an insight into poly(A) site recognition and RNA looping. Yang Q., Coseno M., Gilmartin GM., Doublié S. Only protein-protein interactions imex curation yang-2011-9 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Yang Q., Coseno M., Gilmartin GM., Doublié S. DIP original experiment: DIP-218779X Structure (London, England : 1993) 2011 IMEx Crystal structure of a human cleavage factor CFI(m)25/CFI(m)68/RNA complex provides an insight into poly(A) site recognition and RNA looping. Yang Q., Coseno M., Gilmartin GM., Doublié S. Only protein-protein interactions imex curation yang-2011-10 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Yang Q., Coseno M., Gilmartin GM., Doublié S. DIP original experiment: DIP-218781X Structure (London, England : 1993) 2011 IMEx Crystal structure of a human cleavage factor CFI(m)25/CFI(m)68/RNA complex provides an insight into poly(A) site recognition and RNA looping. Yang Q., Coseno M., Gilmartin GM., Doublié S. Only protein-protein interactions imex curation q16630-1 Cleavage and polyadenylation specificity factor subunit 6 CPSF6 CFIM68 Cleavage and polyadenylation specificity factor 68 kDa subunit Pre-mRNA cleavage factor Im 68 kDa subunit Protein HPBRII-4/7 Cleavage factor Im complex 68 kDa subunit protein protein human Homo sapiens Human MADGVDHIDIYADVGEEFNQEAEYGGHDQIDLYDDVISPSANNGDAPEDRDYMDTLPPTVGDDVGKGAAPNVVYTYTGKRIALYIGNLTWWTTDEDLTEAVHSLGVNDILEIKFFENRANGQSKGFALVGVGSEASSKKLMDLLPKRELHGQNPVVTPCNKQFLSQFEMQSRKTTQSGQMSGEGKAGPPGGSSRAAFPQGGRGRGRFPGAVPGGDRFPGPAGPGGPPPPFPAGQTPPRPPLGPPGPPGPPGPPPPGQVLPPPLAGPPNRGDRPPPPVLFPGQPFGQPPLGPLPPGPPPPVPGYGPPPGPPPPQQGPPPPPGPFPPRPPGPLGPPLTLAPPPHLPGPPPGAPPPAPHVNPAFFPPPTNSGMPTSDSRGPPPTDPYGRPPPYDRGDYGPPGREMDTARTPLSEAEFEEIMNRNRAISSSAISRAVSDASAGDYGSAIETLVTAISLIKQSKVSADDRCKVLISSLQDCLHGIESKSYGSGSRRERSRERDHSRSREKSRRHKSRSRDRHDDYYRERSRERERHRDRDRDRDRERDREREYRHR cpsf5_human Cleavage and polyadenylation specificity factor subunit 5 NUDT21 CFIM25 CPSF25 CPSF5 Cleavage and polyadenylation specificity factor 25 kDa subunit Nucleoside diphosphate-linked moiety X motif 21 Cleavage factor Im complex 25 kDa subunit Nudix hydrolase 21 Pre-mRNA cleavage factor Im 68 kDa subunit protein protein human Homo sapiens Human MSVVPPNRSQTGWPRGVTQFGNKYIQQTKPLTLERTINLYPLTNYTFGTKEPLYEKDSSVAARFQRMREEFDKIGMRRTVEGVLIVHEHRLPHVLLLQLGTTFFKLPGGELNPGEDEVEGLKRLMTEILGRQDGVLQDWVIDDCIGNWWRPNFEPPQYPYIPAHITKPKEHKKLFLVQLQEKALFAVPKNYKLVAAPLFELYDNAPGYGPIISSLPQLLSRFNFIYN cpsf6-nudt21-1 3222237 N/A 3222241 unspecified role unspecified role unspecified role unspecified role RRM domain Region 13-235 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3222242 unspecified role unspecified role unspecified role unspecified role Region 22-227 Region 22-227 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false cpsf6-nudt21-2 3222238 N/A 3222241 unspecified role unspecified role unspecified role unspecified role RRM domain Region 13-235 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3222242 unspecified role unspecified role unspecified role unspecified role Region 22-227 Region 22-227 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false cpsf6-nudt21-3 3222239 N/A 3222241 unspecified role unspecified role unspecified role unspecified role RRM domain Region 13-235 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false region Δ(116-122) required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3222242 unspecified role unspecified role unspecified role unspecified role Region 22-227 Region 22-227 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false O43809:p.[Tyr158Ala;Tyr160Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false cpsf6-nudt21-4 3222240 N/A 3222241 unspecified role unspecified role bait bait RRM domain Region 13-235 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3222242 unspecified role unspecified role prey prey Region 22-227 Region 22-227 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false