DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ fiedler-2011-1 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Fiedler M., Mendoza-Topaz C., Rutherford TJ., Mieszczanek J., Bienz M. DIP original experiment: DIP-249107X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Dishevelled interacts with the DIX domain polymerization interface of Axin to interfere with its function in down-regulating β-catenin. Fiedler M., Mendoza-Topaz C., Rutherford TJ., Mieszczanek J., Bienz M. Only protein-protein interactions imex curation fiedler-2011-2 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Fiedler M., Mendoza-Topaz C., Rutherford TJ., Mieszczanek J., Bienz M. DIP original experiment: DIP-249105X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Dishevelled interacts with the DIX domain polymerization interface of Axin to interfere with its function in down-regulating β-catenin. Fiedler M., Mendoza-Topaz C., Rutherford TJ., Mieszczanek J., Bienz M. Only protein-protein interactions imex curation fiedler-2011-3 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Fiedler M., Mendoza-Topaz C., Rutherford TJ., Mieszczanek J., Bienz M. DIP original experiment: DIP-249109X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Dishevelled interacts with the DIX domain polymerization interface of Axin to interfere with its function in down-regulating β-catenin. Fiedler M., Mendoza-Topaz C., Rutherford TJ., Mieszczanek J., Bienz M. Only protein-protein interactions imex curation fiedler-2011-4 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Fiedler M., Mendoza-Topaz C., Rutherford TJ., Mieszczanek J., Bienz M. DIP original experiment: DIP-249108X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Dishevelled interacts with the DIX domain polymerization interface of Axin to interfere with its function in down-regulating β-catenin. Fiedler M., Mendoza-Topaz C., Rutherford TJ., Mieszczanek J., Bienz M. Only protein-protein interactions imex curation fiedler-2011-5 ecolx Escherichia coli light scattering light scattering predetermined predetermined participant predetermined Fiedler M., Mendoza-Topaz C., Rutherford TJ., Mieszczanek J., Bienz M. DIP original experiment: DIP-249106X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Dishevelled interacts with the DIX domain polymerization interface of Axin to interfere with its function in down-regulating β-catenin. Fiedler M., Mendoza-Topaz C., Rutherford TJ., Mieszczanek J., Bienz M. Only protein-protein interactions imex curation axin1_human Axin-1 Axis inhibition protein 1 AXIN1 AXIN protein protein human Homo sapiens Human MNIQEQGFPLDLGASFTEDAPRPPVPGEEGELVSTDPRPASYSFCSGKGVGIKGETSTATPRRSDLDLGYEPEGSASPTPPYLKWAESLHSLLDDQDGISLFRTFLKQEGCADLLDFWFACTGFRKLEPCDSNEEKRLKLARAIYRKYILDNNGIVSRQTKPATKSFIKGCIMKQLIDPAMFDQAQTEIQATMEENTYPSFLKSDIYLEYTRTGSESPKVCSDQSSGSGTGKGISGYLPTLNEDEEWKCDQDMDEDDGRDAAPPGRLPQKLLLETAAPRVSSSRRYSEGREFRYGSWREPVNPYYVNAGYALAPATSANDSEQQSLSSDADTLSLTDSSVDGIPPYRIRKQHRREMQESVQVNGRVPLPHIPRTYRVPKEVRVEPQKFAEELIHRLEAVQRTREAEEKLEERLKRVRMEEEGEDGDPSSGPPGPCHKLPPAPAWHHFPPRCVDMGCAGLRDAHEENPESILDEHVQRVLRTPGRQSPGPGHRSPDSGHVAKMPVALGGAASGHGKHVPKSGAKLDAAGLHHHRHVHHHVHHSTARPKEQVEAEATRRAQSSFAWGLEPHSHGARSRGYSESVGAAPNASDGLAHSGKVGVACKRNAKKAESGKSASTEVPGASEDAEKNQKIMQWIIEGEKEISRHRRTGHGSSGTRKPQPHENSRPLSLEHPWAGPQLRTSVQPSHLFIQDPTMPPHPAPNPLTQLEEARRRLEEEEKRASRAPSKQRYVQEVMRRGRACVRPACAPVLHVVPAVSDMELSETETRSQRKVGGGSAQPCDSIVVAYYFCGEPIPYRTLVRGRAVTLGQFKELLTKKGSYRYYFKKVSDEFDCGVVFEEVREDEAVLPVFEEKIIGKVEKVD dvl2_human Segment polarity protein dishevelled homolog DVL-2 DSH homolog 2 DVL2 protein protein human Homo sapiens Human MAGSSTGGGGVGETKVIYHLDEEETPYLVKIPVPAERITLGDFKSVLQRPAGAKYFFKSMDQDFGVVKEEISDDNARLPCFNGRVVSWLVSSDNPQPEMAPPVHEPRAELAPPAPPLPPLPPERTSGIGDSRPPSFHPNVSSSHENLEPETETESVVSLRRERPRRRDSSEHGAGGHRTGGPSRLERHLAGYESSSTLMTSELESTSLGDSDEEDTMSRFSSSTEQSSASRLLKRHRRRRKQRPPRLERTSSFSSVTDSTMSLNIITVTLNMEKYNFLGISIVGQSNERGDGGIYIGSIMKGGAVAADGRIEPGDMLLQVNDMNFENMSNDDAVRVLRDIVHKPGPIVLTVAKCWDPSPQAYFTLPRNEPIQPIDPAAWVSHSAALTGTFPAYPGSSSMSTITSGSSLPDGCEGRGLSVHTDMASVTKAMAAPESGLEVRDRMWLKITIPNAFLGSDVVDWLYHHVEGFPERREARKYASGLLKAGLIRHTVNKITFSEQCYYVFGDLSGGCESYLVNLSLNDNDGSSGASDQDTLAPLPGATPWPLLPTFSYQYPAPHPYSPQPPPYHELSSYTYGGGSASSQHSEGSRSSGSTRSDGGAGRTGRPEERAPESKSGSGSESEPSSRGGSLRRGGEASGTSDGGPPPSRGSTGGAPNLRAHPGLHPYGPPPGMALPYNPMMVVMMPPPPPPVPPAVQPPGAPPVRDLGSVPPELTASRQSFHMAMGNPSEFFVDVM axin1-28 3220997 N/A 3221002 unspecified role unspecified role unspecified role unspecified role Dix domain Region 773-862 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false O15169:p.[Ile794Ala;Arg797Asp] I794A/R797D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false axin1-2 3220998 N/A 3221002 unspecified role unspecified role unspecified role unspecified role Dix domain Region 773-862 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false O15169:p.[Ile794Ala;Arg797Asp] I794A/R797D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false O15169:p.Tyr796Asp Y796D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false O15169:p.[Lys825Ala;Glu838Leu] K825A/E838L mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false axin1-dvl2-1 3220999 N/A 3221003 unspecified role unspecified role unspecified role unspecified role Dix domain Region 1-107 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false O14641:p.Tyr27Asp Y27D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3221002 unspecified role unspecified role unspecified role unspecified role Dix domain Region 773-862 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false O15169:p.[Ile794Ala;Arg797Asp] I794A/R797D mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false axin1-dvl2-2 3221000 N/A 3221003 unspecified role unspecified role unspecified role unspecified role Dix domain Region 1-107 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false O14641:p.[Val67Ala;Lys68Ala] V67A/K68A mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3221002 unspecified role unspecified role unspecified role unspecified role Dix domain Region 773-862 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false O15169:p.[Ile794Ala;Arg797Asp] I794A/R797D mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false axin1-3 3221001 N/A 3221002 unspecified role unspecified role unspecified role unspecified role Dix domain Region 773-862 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false O15169:p.[Ile794Ala;Arg797Asp] I794A/R797D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false O15169:p.Tyr796Asp Y796D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false O15169:p.[Lys825Ala;Glu838Leu] K825A/E838L mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false