DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ byrnes-2011-1 in vitro In vitro saxs x ray scattering saxs predetermined predetermined participant predetermined Byrnes LJ., Sondermann H. DIP original experiment: DIP-249654X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Structural basis for the nucleotide-dependent dimerization of the large G protein atlastin-1/SPG3A. Byrnes LJ., Sondermann H. Only protein-protein interactions imex curation byrnes-2011-2 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Byrnes LJ., Sondermann H. DIP original experiment: DIP-249652X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Structural basis for the nucleotide-dependent dimerization of the large G protein atlastin-1/SPG3A. Byrnes LJ., Sondermann H. Only protein-protein interactions imex curation byrnes-2011-3 in vitro In vitro sls static light scattering sls predetermined predetermined participant predetermined Byrnes LJ., Sondermann H. DIP original experiment: DIP-249655X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Structural basis for the nucleotide-dependent dimerization of the large G protein atlastin-1/SPG3A. Byrnes LJ., Sondermann H. Only protein-protein interactions imex curation byrnes-2011-4 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Byrnes LJ., Sondermann H. DIP original experiment: DIP-249653X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Structural basis for the nucleotide-dependent dimerization of the large G protein atlastin-1/SPG3A. Byrnes LJ., Sondermann H. Only protein-protein interactions imex curation atla1_human Atlastin-1 ATL1 GBP3 SPG3A Spastic paraplegia 3 protein A Guanine nucleotide-binding protein 3 GTP-binding protein 3 Brain-specific GTP-binding protein protein protein human Homo sapiens Human MAKNRRDRNSWGGFSEKTYEWSSEEEEPVKKAGPVQVLIVKDDHSFELDETALNRILLSEAVRDKEVVAVSVAGAFRKGKSFLMDFMLRYMYNQESVDWVGDYNEPLTGFSWRGGSERETTGIQIWSEIFLINKPDGKKVAVLLMDTQGTFDSQSTLRDSATVFALSTMISSIQVYNLSQNVQEDDLQHLQLFTEYGRLAMEETFLKPFQSLIFLVRDWSFPYEFSYGADGGAKFLEKRLKVSGNQHEELQNVRKHIHSCFTNISCFLLPHPGLKVATNPNFDGKLKEIDDEFIKNLKILIPWLLSPESLDIKEINGNKITCRGLVEYFKAYIKIYQGEELPHPKSMLQATAEANNLAAVATAKDTYNKKMEEICGGDKPFLAPNDLQTKHLQLKEESVKLFRGVKKMGGEEFSRRYLQQLESEIDELYIQYIKHNDSKNIFHAARTPATLFVVIFITYVIAGVTGFIGLDIIASLCNMIMGLTLITLCTWAYIRYSGEYRELGAVIDQVAAALWDQGSTNEALYKLYSAAATHRHLYHQAFPTPKSESTEQSEKKKM atl1-1 3220740 N/A 3220744 unspecified role unspecified role unspecified role unspecified role Cytoplasmic domain Region 1-446 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false atl1-4 3220741 N/A 3220744 unspecified role unspecified role unspecified role unspecified role Cytoplasmic domain Region 1-446 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false atl1-5 3220742 N/A 3220744 unspecified role unspecified role unspecified role unspecified role Cytoplasmic domain Region 1-446 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q8WXF7:p.Arg77Glu R77E mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q8WXF7:p.Gln191Arg Q191R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q8WXF7:p.Arg217Gln R217Q mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q8WXF7:p.Tyr336His Y336H mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q8WXF7:p.Met408Thr M408T/V mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q8WXF7:p.Met408Val mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false atl1-6 3220743 N/A 3220744 unspecified role unspecified role unspecified role unspecified role Cytoplasmic domain Region 1-446 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q8WXF7:p.Arg77Glu R77E mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q8WXF7:p.Gln191Arg Q191R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q8WXF7:p.Arg217Gln R217Q mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q8WXF7:p.Tyr336His Y336H mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q8WXF7:p.Met408Thr M408T/V mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q8WXF7:p.Met408Val mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false