DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ tsai-2010-4 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. DIP original experiment: DIP-254409X Nature 2010 IMEx TRIM24 links a non-canonical histone signature to breast cancer. Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. Only protein-protein interactions imex curation tsai-2010-5 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. DIP original experiment: DIP-254419X Nature 2010 IMEx TRIM24 links a non-canonical histone signature to breast cancer. Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. Only protein-protein interactions imex curation tsai-2010-6 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. DIP original experiment: DIP-254423X Nature 2010 IMEx TRIM24 links a non-canonical histone signature to breast cancer. Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. Only protein-protein interactions imex curation tsai-2010-7 in vitro In vitro peptide array peptide array predetermined predetermined participant predetermined Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. DIP original experiment: DIP-254413X Nature 2010 IMEx TRIM24 links a non-canonical histone signature to breast cancer. Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. Only protein-protein interactions imex curation tsai-2010-8 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. DIP original experiment: DIP-254416X Nature 2010 IMEx TRIM24 links a non-canonical histone signature to breast cancer. Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. Only protein-protein interactions imex curation tsai-2010-9 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. DIP original experiment: DIP-254410X Nature 2010 IMEx TRIM24 links a non-canonical histone signature to breast cancer. Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. Only protein-protein interactions imex curation tsai-2010-10 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. DIP original experiment: DIP-254414X Nature 2010 IMEx TRIM24 links a non-canonical histone signature to breast cancer. Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. Only protein-protein interactions imex curation tsai-2010-11 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. DIP original experiment: DIP-254422X Nature 2010 IMEx TRIM24 links a non-canonical histone signature to breast cancer. Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. Only protein-protein interactions imex curation tsai-2010-12 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. DIP original experiment: DIP-254417X Nature 2010 IMEx TRIM24 links a non-canonical histone signature to breast cancer. Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. Only protein-protein interactions imex curation tsai-2010-13 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. DIP original experiment: DIP-254420X Nature 2010 IMEx TRIM24 links a non-canonical histone signature to breast cancer. Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. Only protein-protein interactions imex curation tsai-2010-14 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. DIP original experiment: DIP-254411X Nature 2010 IMEx TRIM24 links a non-canonical histone signature to breast cancer. Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. Only protein-protein interactions imex curation tsai-2010-15 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. DIP original experiment: DIP-254415X Nature 2010 IMEx TRIM24 links a non-canonical histone signature to breast cancer. Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. Only protein-protein interactions imex curation tsai-2010-16 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. DIP original experiment: DIP-254412X Nature 2010 IMEx TRIM24 links a non-canonical histone signature to breast cancer. Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. Only protein-protein interactions imex curation tsai-2010-17 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. DIP original experiment: DIP-254421X Nature 2010 IMEx TRIM24 links a non-canonical histone signature to breast cancer. Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. Only protein-protein interactions imex curation tsai-2010-18 in vitro In vitro fps fluorescence polarization spectroscopy Fluorescence anisotropy FPS FPS Fluorescence anisotropy fps predetermined predetermined participant predetermined Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. DIP original experiment: DIP-254418X Nature 2010 IMEx TRIM24 links a non-canonical histone signature to breast cancer. Tsai WW., Wang Z., Yiu TT., Akdemir KC., Xia W., Winter S., Tsai CY., Shi X., Schwarzer D., Plunkett W., Aronow B., Gozani O., Fischle W., Hung MC., Patel DJ., Barton MC. Only protein-protein interactions imex curation h3(133) Histone 3 peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) ARTKQTARKSTGGKAPRKQLATKAARKSAPATGYK tif1a_human Transcription intermediary factor 1-alpha TRIM24 RNF82 TIF1 TIF1A Tripartite motif-containing protein 24 RING finger protein 82 E3 ubiquitin-protein ligase TRIM24 RING-type E3 ubiquitin transferase TIF1-alpha protein protein human Homo sapiens Human MEVAVEKAVAAAAAASAAASGGPSAAPSGENEAESRQGPDSERGGEAARLNLLDTCAVCHQNIQSRAPKLLPCLHSFCQRCLPAPQRYLMLPAPMLGSAETPPPVPAPGSPVSGSSPFATQVGVIRCPVCSQECAERHIIDNFFVKDTTEVPSSTVEKSNQVCTSCEDNAEANGFCVECVEWLCKTCIRAHQRVKFTKDHTVRQKEEVSPEAVGVTSQRPVFCPFHKKEQLKLYCETCDKLTCRDCQLLEHKEHRYQFIEEAFQNQKVIIDTLITKLMEKTKYIKFTGNQIQNRIIEVNQNQKQVEQDIKVAIFTLMVEINKKGKALLHQLESLAKDHRMKLMQQQQEVAGLSKQLEHVMHFSKWAVSSGSSTALLYSKRLITYRLRHLLRARCDASPVTNNTIQFHCDPSFWAQNIINLGSLVIEDKESQPQMPKQNPVVEQNSQPPSGLSSNQLSKFPTQISLAQLRLQHMQQQVMAQRQQVQRRPAPVGLPNPRMQGPIQQPSISHQQPPPRLINFQNHSPKPNGPVLPPHPQQLRYPPNQNIPRQAIKPNPLQMAFLAQQAIKQWQISSGQGTPSTTNSTSSTPSSPTITSAAGYDGKAFGSPMIDLSSPVGGSYNLPSLPDIDCSSTIMLDNIVRKDTNIDHGQPRPPSNRTVQSPNSSVPSPGLAGPVTMTSVHPPIRSPSASSVGSRGSSGSSSKPAGADSTHKVPVVMLEPIRIKQENSGPPENYDFPVVIVKQESDEESRPQNANYPRSILTSLLLNSSQSSTSEETVLRSDAPDSTGDQPGLHQDNSSNGKSEWLDPSQKSPLHVGETRKEDDPNEDWCAVCQNGGELLCCEKCPKVFHLSCHVPTLTNFPSGEWICTFCRDLSKPEVEYDCDAPSHNSEKKKTEGLVKLTPIDKRKCERLLLFLYCHEMSLAFQDPVPLTVPDYYKIIKNPMDLSTIKKRLQEDYSMYSKPEDFVADFRLIFQNCAEFNEPDSEVANAGIKLENYFEELLKNLYPEKRFPKPEFRNESEDNKFSDDSDDDFVQPRKKRLKSIEERQLLK esr1_human Estrogen receptor ESR1 ESR NR3A1 Estradiol receptor ER-alpha Nuclear receptor subfamily 3 group A member 1 protein protein human Homo sapiens Human MTMTLHTKASGMALLHQIQGNELEPLNRPQLKIPLERPLGEVYLDSSKPAVYNYPEGAAYEFNAAAAANAQVYGQTGLPYGPGSEAAAFGSNGLGGFPPLNSVSPSPLMLLHPPPQLSPFLQPHGQQVPYYLENEPSGYTVREAGPPAFYRPNSDNRRQGGRERLASTNDKGSMAMESAKETRYCAVCNDYASGYHYGVWSCEGCKAFFKRSIQGHNDYMCPATNQCTIDKNRRKSCQACRLRKCYEVGMMKGGIRKDRRGGRMLKHKRQRDDGEGRGEVGSAGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSLQKYYITGEAEGFPATV ing1_human Inhibitor of growth protein 1 ING1 protein protein human Homo sapiens Human MSFVECPYHSPAERLVAEADEGGPSAITGMGLCFRCLLFSFSGRSGVEGGRVDLNVFGSLGLQPWIGSSRCWGGPCSSALRCGWFSSWPPPSKSAIPIGGGSRGAGRVSRWPPPHWLEAWRVSPLPLSPLSPATFGRGFIAVAVIPGLWARGRGCSSDRLPRPAGPARRQFQAASLLTRGWGRAWPWKQILKELDECYERFSRETDGAQKRRMLHCVQRALIRSQELGDEKIQIVSQMVELVENRTRQVDSHVELFEAQQELGDTAGNSGKAGADRPKGEAAAQADKPNSKRSRRQRNNENRENASSNHDHDDGASGTPKEKKAKTSKKKKRSKAKAEREASPADLPIDPNEPTYCLCNQVSYGEMIGCDNDECPIEWFHFSCVGLNHKPKGKWYCPKCRGENEKTMDKALEKSKKERAYNR h4(127) Histone 4 peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) SGRGKGGKGLGKGGAKRHRKVLRDNIQ pf21a_human PHD finger protein 21A BRAF35-HDAC complex protein BHC80 BHC80a PHF21A BHC80 KIAA1696 BM-006 protein protein human Homo sapiens Human MELQTLQEALKVEIQVHQKLVAQMKQDPQNADLKKQLHELQAKITALSEKQKRVVEQLRKNLIVKQEQPDKFQIQPLPQSENKLQTAQQQPLQQLQQQQQYHHHHAQQSAAASPNLTASQKTVTTASMITTKTLPLVLKAATATMPASVVGQRPTIAMVTAINSQKAVLSTDVQNTPVNLQTSSKVTGPGAEAVQIVAKNTVTLVQATPPQPIKVPQFIPPPRLTPRPNFLPQVRPKPVAQNNIPIAPAPPPMLAAPQLIQRPVMLTKFTPTTLPTSQNSIHPVRVVNGQTATIAKTFPMAQLTSIVIATPGTRLAGPQTVQLSKPSLEKQTVKSHTETDEKQTESRTITPPAAPKPKREENPQKLAFMVSLGLVTHDHLEEIQSKRQERKRRTTANPVYSGAVFEPERKKSAVTYLNSTMHPGTRKRGRPPKYNAVLGFGALTPTSPQSSHPDSPENEKTETTFTFPAPVQPVSLPSPTSTDGDIHEDFCSVCRKSGQLLMCDTCSRVYHLDCLDPPLKTIPKGMWICPRCQDQMLKKEEAIPWPGTLAIVHSYIAYKAAKEEEKQKLLKWSSDLKQEREQLEQKVKQLSNSISKCMEMKNTILARQKEMHSSLEKVKQLIRLIHGIDLSKPVDSEATVGAISNGPDCTPPANAATSTPAPSPSSQSCTANCNQGEETK h3(133)-trim24-1 3219257 N/A 3219272 unspecified role unspecified role unspecified role unspecified role Region 1-10 Region 1-10 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3219273 unspecified role unspecified role unspecified role unspecified role PHD-Bromo domain Region 824-1006 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false h3(133)-trim24-2 3219258 N/A 3219272 unspecified role unspecified role unspecified role unspecified role Region 13-32 Region 13-32 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3219273 unspecified role unspecified role unspecified role unspecified role Bromo domain Region 896-1016 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false trim24-esr1-1 3219259 N/A 3219274 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3219273 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association false false false h3(133)-trim24-3 3219260 N/A 3219272 unspecified role unspecified role bait bait Region 1-21 Region 1-21 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Biotin-tag Biotin-tag biotin tag biotin tag c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal false in vitro In vitro N/A 3219273 unspecified role unspecified role prey prey PHD-Bromo domain Region 824-1011 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false h3(133)-ing1-1 3219261 N/A 3219275 unspecified role unspecified role prey prey PHD domain region 353-402 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3219272 unspecified role unspecified role bait bait Region 1-21 Region 1-21 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Biotin-tag Biotin-tag biotin tag biotin tag c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal false K4me1 K4me1 n6me1lys N6-methyl-L-lysine (2S)-2-amino-6-methylaminohexanoic acid MOD_RES N6-methyllysine N(zeta)-methyllysine N6-methyl-L-lysine N6-methylated L-lysine epsilon-methyllysine Methyl Methylation N-methyl Lysyl N6Me1Lys certain certain sequence position certain certain certain sequence position certain false K4me2 K4me2 n6me2lys N6,N6-dimethyl-L-lysine (2S)-2-amino-6-(dimethylamino)hexanoic acid MOD_RES N6,N6-dimethyllysine N(zeta)-dimethyllysine N6,N6-dimethyl-L-lysine N6,N6-dimethylated L-lysine epsilon-dimethyllysine lysine derivative Lys(y) Dimethyl N6Me2Lys di-Methylation dimethylk certain certain sequence position certain certain certain sequence position certain false K4me3 K4me3 n6me3+lys N6,N6,N6-trimethyl-L-lysine (5S)-5-amino-5-carboxy-N,N,N-trimethylpentan-1-aminium 2-amino-6-(trimethylammonio)hexanoic acid 5-azanyl-5-carboxy-N,N,N-trimethylpentanazanium MOD_RES N6,N6,N6-trimethyllysine N(zeta)-trimethyllysine N6,N6,N6-trimethyl-L-lysine N6,N6,N6-trimethylated L-lysine N6,N6,N6-trimethyllysin-N6-ium N6,N6,N6-trimethyllysine cation epsilon-trimethyllysine N-trimethylation (of lysine) N6Me3+Lys Trimethyl tri-Methylation certain certain sequence position certain certain certain sequence position certain false K9me2 K9me2 n6me2lys N6,N6-dimethyl-L-lysine (2S)-2-amino-6-(dimethylamino)hexanoic acid MOD_RES N6,N6-dimethyllysine N(zeta)-dimethyllysine N6,N6-dimethyl-L-lysine N6,N6-dimethylated L-lysine epsilon-dimethyllysine lysine derivative Lys(y) Dimethyl N6Me2Lys di-Methylation dimethylk certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false h3(133)-trim24-4 3219262 N/A 3219272 unspecified role unspecified role unspecified role unspecified role Region 13-32 Region 13-32 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3219273 unspecified role unspecified role unspecified role unspecified role PHD-Bromo domain Region 824-1006 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false h3(133)-trim24-5 3219263 N/A 3219272 unspecified role unspecified role bait bait Region 1-21 Region 1-21 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Biotin-tag Biotin-tag biotin tag biotin tag c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal false in vitro In vitro N/A 3219273 unspecified role unspecified role prey prey PHD domain Region 824-886 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false O15164:p.Cys840Trp C840W mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false trim24-esr1-2 3219264 N/A 3219274 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3219273 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli physical association physical association false false false h3(133)-trim24-6 3219265 N/A 3219272 unspecified role unspecified role unspecified role unspecified role Region 1-15 Region 1-15 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Region 13-32 Region 13-32 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3219273 unspecified role unspecified role unspecified role unspecified role PHD-Bromo domain Region 824-1011 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false O15164:p.Asp827Ala D827A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O15164:p.Cys840Trp C840W mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O15164:p.[Phe979Ala;Asn980Ala] F979A/N980A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false h4(127)-trim24-1 3219266 N/A 3219276 unspecified role unspecified role bait bait K5ac K5ac naclys N-acetylated L-lysine NAcLys certain certain sequence position certain certain certain sequence position certain false K8ac K8ac naclys N-acetylated L-lysine NAcLys certain certain sequence position certain certain certain sequence position certain false K12ac K12ac naclys N-acetylated L-lysine NAcLys certain certain sequence position certain certain certain sequence position certain false K16ac K16ac naclys N-acetylated L-lysine NAcLys certain certain sequence position certain certain certain sequence position certain false K20ac K20ac naclys N-acetylated L-lysine NAcLys certain certain sequence position certain certain certain sequence position certain false Biotin-tag Biotin-tag biotin tag biotin tag c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal false in vitro In vitro N/A 3219273 unspecified role unspecified role prey prey GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false O15164:p.[Phe979Ala;Asn980Ala] F979A/N980A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false PHD-Bromo domain Region 824-1011 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false h3(133)-trim24-7 3219267 N/A 3219272 unspecified role unspecified role unspecified role unspecified role Region 23-31 Region 23-31 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3219273 unspecified role unspecified role unspecified role unspecified role PHD-Bromo domain Region 824-1006 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false h3(133)-phf21a-1 3219268 N/A 3219277 unspecified role unspecified role prey prey PHD domain Region 488-535 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3219272 unspecified role unspecified role bait bait Region 1-21 Region 1-21 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Biotin-tag Biotin-tag biotin tag biotin tag c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal false in vitro In vitro direct interaction direct interaction false false false h4(127)-trim24-2 3219269 N/A 3219276 unspecified role unspecified role unspecified role unspecified role Region 14-19 Region 14-19 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3219273 unspecified role unspecified role unspecified role unspecified role PHD-Bromo domain Region 824-1006 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false trim24-trim24-1 3219270 N/A 3219273 unspecified role unspecified role unspecified role unspecified role Bromo domain Region 896-1016 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3219273 unspecified role unspecified role unspecified role unspecified role PHD domain Region 824-886 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false h3(133)-trim24-8 3219271 N/A 3219272 unspecified role unspecified role unspecified role unspecified role Fluorescein-label Fluorescein-label fluorescein fluorescein label fluorescein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro N/A 3219273 unspecified role unspecified role unspecified role unspecified role O15164:p.Cys840Trp C840W mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false O15164:p.[Phe979Ala;Asn980Ala] F979A/N980A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false PHD-Bromo domain Region 824-1011 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false