DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ robertson-2010-1 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Robertson JL., Kolmakova-Partensky L., Miller C. DIP original experiment: DIP-219266X Nature 2010 IMEx Design, function and structure of a monomeric ClC transporter. Robertson JL., Kolmakova-Partensky L., Miller C. Only protein-protein interactions imex curation robertson-2010-2 in vitro In vitro crosslink cross-linking study crosslink weight by comassie molecular weight estimation by coomasie staining weight by comassie Robertson JL., Kolmakova-Partensky L., Miller C. DIP original experiment: DIP-219267X Nature 2010 IMEx Design, function and structure of a monomeric ClC transporter. Robertson JL., Kolmakova-Partensky L., Miller C. Only protein-protein interactions imex curation robertson-2010-3 in vitro In vitro crosslink cross-linking study crosslink weight silver stain molecular weight estimation by silver staining weight silver stain Robertson JL., Kolmakova-Partensky L., Miller C. DIP original experiment: DIP-219268X Nature 2010 IMEx Design, function and structure of a monomeric ClC transporter. Robertson JL., Kolmakova-Partensky L., Miller C. Only protein-protein interactions imex curation clca_ecoli H(+)/Cl(-) exchange transporter ClcA clcA ClC-ec1 eriC yadQ JW5012 b0155 protein protein ecoli Escherichia coli (strain K12) MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGTLVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAPEAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGRMVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAVFIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGMQDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLVFIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIAGMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILARTLAKQEAEQLARSKAASASENT 0B1BC39B417E9690 clca-1 3217239 N/A 3217242 unspecified role unspecified role unspecified role unspecified role P37019:p.Ile422Trp I422W mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P37019:p.[Ile201Trp;Ile422Trp] I201W/I422W mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P37019:p.Ile201Trp I201W mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P37019:p.Leu434Trp I434W mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P37019:p.Leu406Trp L406W mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false clca-2 3217240 N/A 3217242 unspecified role unspecified role unspecified role unspecified role P37019:p.[Ile201Trp;Ile422Trp] I201W/I422W mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false clca-3 3217241 N/A 3217242 unspecified role unspecified role unspecified role unspecified role P37019:p.[Ile201Trp;Ile422Trp] I201W/I422W mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false