DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ nataf-2010-1 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Nataf Y., Bahari L., Kahel-Raifer H., Borovok I., Lamed R., Bayer EA., Sonenshein AL., Shoham Y. DIP original experiment: DIP-220468X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Clostridium thermocellum cellulosomal genes are regulated by extracytoplasmic polysaccharides via alternative sigma factors. Nataf Y., Bahari L., Kahel-Raifer H., Borovok I., Lamed R., Bayer EA., Sonenshein AL., Shoham Y. Only protein-protein interactions imex curation nataf-2010-2 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Nataf Y., Bahari L., Kahel-Raifer H., Borovok I., Lamed R., Bayer EA., Sonenshein AL., Shoham Y. DIP original experiment: DIP-220469X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Clostridium thermocellum cellulosomal genes are regulated by extracytoplasmic polysaccharides via alternative sigma factors. Nataf Y., Bahari L., Kahel-Raifer H., Borovok I., Lamed R., Bayer EA., Sonenshein AL., Shoham Y. Only protein-protein interactions imex curation nataf-2010-3 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Nataf Y., Bahari L., Kahel-Raifer H., Borovok I., Lamed R., Bayer EA., Sonenshein AL., Shoham Y. DIP original experiment: DIP-220470X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Clostridium thermocellum cellulosomal genes are regulated by extracytoplasmic polysaccharides via alternative sigma factors. Nataf Y., Bahari L., Kahel-Raifer H., Borovok I., Lamed R., Bayer EA., Sonenshein AL., Shoham Y. Only protein-protein interactions imex curation rsgi1_acet2 Anti-sigma-I factor RsgI1 rsgI1 Cthe_0059 protein protein cloth Clostridium thermocellum (strain ATCC 27405 / DSM 1237) Ruminiclostridium thermocellum MNRLGIIYEIQGMKAVVLTSEGEFLIIRRRKDMKVGQQVSFENEDIYNVRGKRFLYVAAAVSSVAAVLVVMFLYFQSAFLSNTDNIYGYICVDINPSVELVIDETCRVLEVRPQNKDGEQLISGLELLDKNVEDVVYELINRSISFGFVKADDNRKIVLISGALNDKRNELKTKKENDEAELTELLDNIKARVDRIDNIKVRTITATSRERKDALKYGLSMGKYCLYLEAQELNGSITIDEVHDMSISDMIEKLEQMKLALKDEASPKLQTTPTLGGETAQISPESMQHSTVPGLPETPSSSEKTIAPTLHGTPGVPDEKTLQPSTPTESSEYVQDGTKGLKIQYYSRKPHDSAGIDFSFRMFNTGNEAIDLKDVKVRYYFKEDVSIDEMNWAVYFYSLGSEKDVQCRFYELPGKKEANKYLEITFKSGTLSPNDVMYITGEFYKNDWTKFEQRDDYSYNPADSYSDWKRMTAYISNKLVWGIEPN sigi1_acet2 RNA polymerase sigma factor SigI1 sigI1 Cthe_0058 protein protein cloth Clostridium thermocellum (strain ATCC 27405 / DSM 1237) Ruminiclostridium thermocellum MEVRKINTHNREKKLDDIVYDDFISTLRQIKEGNHQLREEFISEYKPFILKVTSNATGKYIDTRNSDEFSIALSAFNEAIDKFDIEKGYNFFLFSEQVIRRRLIDYSRSNKDDKEYPFSFFDDEYFYNNEKLLSKSYIGFEDIEAREDIEELKKKLQEFGITFLDLVLNVPKHRDSRQLCIRLAKMLAEDEQMYNALMKNKNIPRNELKKKAKVHGRTIGNNRKYIIALCLIFRSNLNLSKRYLEYYTMDGESDLI rsgi2_acet2 Anti-sigma-I factor RsgI2 rsgI2 Cthe_0267 protein protein cloth Clostridium thermocellum (strain ATCC 27405 / DSM 1237) Ruminiclostridium thermocellum MSHYTGIILKLESDRAIVLTDGLDFMELKLKPGMQRGQHVIFDESDLYSAGLITRYKSIIMPFSAFAAAAAVFLVILFSLRFVSISQEYAYIDVDINPSIGLVIDKKEKVIDAKPLNNDAKPILDEAAPKDMPLYDALSKILDISKKNGYINSADNIVLFSASINSGRNNVSESDKGIQEIISTLKDVAKDAGVKFEIIPSTEEDRQKALDQNLSMGRYAIYVKAVEEGVNLNLEDARNLSVSEILGKVNIGKFAISDTPEDSGIMPAISVPAEPVPSVTPAYTAVPEKTEAQPVDIPKSSPTPASFTAHVPTPPKTPSIPHTSGPAIVHTPAADKTTPTFTGSSTPVPTNVVAIASTPVPVSTPKPVSTPAYSSTPTPESTPVPVSTPKPASTPTPASTPKPVSTPTHVSTPKPISTPTSTPRPASTPKPTSTPTPESTPKPTSTPAPVSTPTSTPIPTYTSTPASTPIPAYTSTPTSIPTLTPATSPAPTSSPTPIPSPAPTETDLLTKIELQAYNHIRTSETKELQPRIKLINTGNTPITLSEVKIRYYYTKDQVINEIYTCDWSNITSSKITGTVVQMSNPKPNADSYVEIGFTNSAGVLNPGEYVEIISRIGNSYALSLATPPYSEWNYMYDQNSDYSFNNSSSDFVVWDKITVYISGTLYWGIEP sigi2_acet2 RNA polymerase sigma factor SigI2 sigI2 Cthe_0268 protein protein cloth Clostridium thermocellum (strain ATCC 27405 / DSM 1237) Ruminiclostridium thermocellum MIDLFSPKGKKDTVSTTNKDKSFEDGIVNIINKIKAGDKLLREEFINSYTPYIIRTVSNLTGKYVDVENSDEFSVGLAAFNEAIDSFDEGKNMFFFKFSTLVIKRRLTDYARHNKKHCHVYPFTYFEDKNNSYFEQIHLKSEIDLQNTYEISREIELYEQKLRDFGISLEHLAKCAPKHKDSKSLCIKIAKVIADNKELFSKLERTRNIPKTELLKLLKINKKTIERNRTFIIAAALIFGNDFNLLKDFLDISEPGGDNIERSTK rsgi6_acet2 Anti-sigma-I factor RsgI6 rsgI6 Endo-1,4-beta-xylanase Cthe_2119 protein protein cloth Clostridium thermocellum (strain ATCC 27405 / DSM 1237) Ruminiclostridium thermocellum MIVGKVLDMDEKTAIIMTDDFAFLNVVRTSEMAVGKKVKVLDSDIIKPKNSLRRYLPVAAVAACFVIVLSFVLMFINGNTARKNIYAYVGIDINPSIELWINYNNKIAEAKALNGDAETVLEGLELKEKTVAEAVNEIVQKSMELGFISREKENIILISTACDLKAGEGSENKDVQNKIGQLFDDVNKAVSDLKNSGITTRILNLTLEERESSKEENISMGRYAVYLKAKEQNVNLTIDEIKDADLLELIAKVGIDNENVPEDIVTEDKDNLDAINTGPAESAVPEVTETLPATSTPGRTEGNTATGSVDSTPALSKNETPGKTETPGRTFNTPAKSSLGQSSTPKPVSPVQTATATKGIGTLTPRNSPTPVIPSTGIQWIDQANERINEIRKRNVQIKVVDSSNKPIENAYVEAVLTNHAFGFGTAITRRAMYDSNYTKFIKDHFNWAVFENESKWYTNEPSMGIITYDDADYLYEFCRSNGIKVRGHCIFWEAEEWQPAWVRSLDPFTLRFAVDNRLNSAVGHFKGKFEHWDVNNEMIHGNFFKSRLGESIWPYMFNRAREIDPNAKYFVNNNITTLKEADDCVALVNWLRSQGVRVDGVGVHGHFGDSVDRNLLKGILDKLSVLNLPIWITEYDSVTPDEYRRADNLENLYRTAFSHPSVEGIVMWGFWERVHWRGRDASIVNDNWTLNEAGRRFESLMNEWTTRAYGSTDGSGSFGFRGFYGTYRITVTVPGKGKYNYTLNLNRGSGTLQTTYRIP sigi6_acet2 RNA polymerase sigma factor SigI6 sigI6 Cthe_2120 protein protein cloth Clostridium thermocellum (strain ATCC 27405 / DSM 1237) Ruminiclostridium thermocellum MDWHFQGTNDDREHTKRIIIEYLNRIKAGDDSAREEFILRFRPFILKLVYKATDRHVEPENSEEYSVALLAFNEAINAYDEEKHSNFLVFSEQVINRRLIDYKRKNHKNKMVYPFSYFENEDIKLERTLSDADGNNAIERLEFTDEIRLFKSELASFDITFKDLLSCTPKHRDSRELLINIAKKIASNDGLYEKLKKTKKLPTLELLKLAKVSRRTIERNKKYIIAVSLILRSNLEIFKEYAAGIQEKEVDLR rsgi1-sigi1-1 3215669 N/A 3215672 unspecified role unspecified role unspecified role unspecified role Anti-sigma domain Region 1-52 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3215673 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false rsgi2-sigi2-1 3215670 N/A 3215674 unspecified role unspecified role unspecified role unspecified role Anti-sigma domain Region 1-67 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3215675 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false rsgi6-sigi6-1 3215671 N/A 3215676 unspecified role unspecified role unspecified role unspecified role Anti-sigma domain Region 1-54 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3215677 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false