DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ iwai-2010-1 chlre Chlamydomonas reinhardtii Chlamydomonas smithii pull down pull down western blot western blot Immuno blot Immuno blot Iwai M., Takizawa K., Tokutsu R., Okamuro A., Takahashi Y., Minagawa J. DIP original experiment: DIP-216288X Nature 2010 IMEx Isolation of the elusive supercomplex that drives cyclic electron flow in photosynthesis. Iwai M., Takizawa K., Tokutsu R., Okamuro A., Takahashi Y., Minagawa J. Only protein-protein interactions imex curation iwai-2010-2 chlre Chlamydomonas reinhardtii Chlamydomonas smithii molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Iwai M., Takizawa K., Tokutsu R., Okamuro A., Takahashi Y., Minagawa J. DIP original experiment: DIP-216289X Nature 2010 IMEx Isolation of the elusive supercomplex that drives cyclic electron flow in photosynthesis. Iwai M., Takizawa K., Tokutsu R., Okamuro A., Takahashi Y., Minagawa J. Only protein-protein interactions imex curation psaf_chlre Photosystem I reaction center subunit III, chloroplastic Light-harvesting complex I 17 kDa protein PSI-F P21 protein PSAF protein protein chlre Chlamydomonas reinhardtii Chlamydomonas smithii MALTMRNPAVKASSRVAPSSRRALRVACQAQKNETASKVGTALAASALAAAVSLSAPSAAMADIAGLTPCSESKAYAKLEKKELKTLEKRLKQYEADSAPAVALKATMERTKARFANYAKAGLLCGNDGLPHLIADPGLALKYGHAGEVFIPTFGFLYVAGYIGYVGRQYLIAVKGEAKPTDKEIIIDVPLATKLAWQGAGWPLAAVQELQRGTLLEKEENITVSPR psaa_chlre Photosystem I P700 chlorophyll a apoprotein A1 psaA ps1a1 PSI-A PsaA protein protein chlre Chlamydomonas reinhardtii Chlamydomonas smithii MTISTPEREAKKVKIAVDRNPVETSFEKWAKPGHFSRTLSKGPNTTTWIWNLHADAHDFDSHTSDLEEISRKVFSAHFGQLGIIFIWLSGMYFHGARFSNYEAWLSDPTHIKPSAQVVWPIVGQEILNGDVGGGFQGIQITSGFFQLWRASGITSELQLYTTAIGGLVMAAAMFFAGWFHYHKAAPKLEWFQNVESMLNHHLGGLLGLGSLAWAGHQIHVSLPVNKLLDAGVDPKEIPLPHDLLLNRAIMADLYPSFAKGIAPFFTLNWSEYSDFLTFKGGLNPVTGGLWLSDTAHHHVAIAVLFLVAGHMYRTNWGIGHSMKEILEAHRGPFTGEGHVGLYEILTTSWHAQLAINLALFGSLSIIVAHHMYAMPPYPYLATDYGTQLSLFTHHTWIGGFCIVGAGAHAAIFMVRDYDPTNNYNNLLDRVIRHRDAIISHLNWVCIFLGFHSFGLYIHNDTMSALGRPQDMFSDTAIQLQPVFAQWIQNTHFLAPQLTAPNALAATSLTWGGDLVAVGGKVAMMPISLGTSDFMVHHIHAFTIHVTVLILLKGVLFARSSRLIPDKANLGFRFPCDGPGRGGTCQVSAWDHVFLGLFWMYNSLSIVIFHFSWKMQSDVWGTVTASGVSHITGGNFAQSANTINGWLRDFLWAQSSQVIQSYGSALSAYGLIFLGAHFVWAFSLMFLFSGRGYWQELIESIVWAHNKLKVAPAIQPRALSITQGRAVGVAHYLLGGIATTWSFFLARIISVG a8itk6_chlre TEF3 CHLREDRAFT_183448 CHLRE_07g340200v5 protein protein chlre Chlamydomonas reinhardtii Chlamydomonas smithii MQTTVLRSSTRVGCVRPVGLARALPVRPVVFGSRTPMLLRSAKKDDGYISEDEGLGNVAADYCAIDGAGKKAKRSLGEMEQEFLAAMTSWYYEGKPTMSDEEFSLLKEELIWSGSMVAVLSSDEQRFLEASMAYAKGKPIMTDEDYDALKAELRNKSSIVTAQGPRCSIRSKKMYADAEPDYLRMTALNLPGVLFVLGLVFAVDYSTGFGVTKLVELPAPYGPILLWGLLLPSLFTVAYALTQVGFKDNLILKAPCPSCGSENFSYFGDVFTVAGARGQNLVECPNCKADMIFDEYKRVVVVAETSEVKQEKLAAAAAKKAAAAAKKKAKAAA fenr_chlre Ferredoxin--NADP reductase, chloroplastic PETH FNR protein protein chlre Chlamydomonas reinhardtii Chlamydomonas smithii MASLRKPSNHADRACSRRLRVATRVAGRRMCRPVAATKASTAVTTDMSKRTVPTKLEEGEMPLNTYSNKAPFKAKVRSVEKITGPKATGETCHIIIETEGKIPFWEGQSYGVIPPGTKINSKGKEVPTARLYSIASSRYGDDGDGQTASLCVRRAVYVDPETGKEDPAKKGLCSNFLCDATPGTEISMTGPTGKVLLLPADANAPLICVATGTGIAPFRSFWRRCFIENVPSYKFTGLFWLFMGVGNSDAKLYDEELQAIAKAYPGQFRLDYALSREQNNRKGGKMYIQDKVEEYADEIFDLLDNGAHMYFCGLKGMMPGIQDMLERVAKEKGLNYEEWVEGLKHKNQWHVEVY cb29_chlre Chlorophyll a-b binding protein CP29 Lhcb4 Lhcbm4 protein protein chlre Chlamydomonas reinhardtii Chlamydomonas smithii MVFKFPTPPGTQKKAGTTATKPAPKATTKKVATSTGTRSGGVGYRKYQGDALWLPNTTRPEWLDGSLPGDRGFDPLGLSKPSEFVVIGVDENDQNAAKNNKGSVEAIVQATPDEVSSENRLAPYSEVFGLARFRECELIHGRWAMLACLGALVAEATTGVSWVEAGKVELDGASYAGLSLPFSITQLIWIEVILVGGAEFYRNSETNPEKRCYPGGVFDPLKLASEDEERAFRLKTAEIKHARLAMVSFFGYGVQALSTGEGALGSLAKFADGLNNGKGL q9fek6_chlre Chlorophyll a-b binding protein, chloroplastic lhcb5 LHCB5 CHLREDRAFT_184397 CHLRE_16g673650v5 protein protein chlre Chlamydomonas reinhardtii Chlamydomonas smithii MQIQALFKKTGASAPAKKGTASTKVVKPSKAGGKATRGWLGGQGGAADLDKWYGPDRKLFLPSGLYDRSEIPEYLNGELAGDYGYDPLGLGKDPETVAKYRENELLHARWAMLAAAGILIPEGLQANGANIKGGTWFETGAEMLNGGTLNYFAVPWGIVSNPLPLFAVIAVEVGLMGAVEFYRRNGTGPAGYSPGIGKFDSSVFDGLDPLYPGGPFDPLGLADDPEVLQELKVKEIKNGRLAMVSVLGFAVQSYVTGEGPYANWTKHVADPFGYNLLTVLGAEERTPTL q9zsj4_chlre Chlorophyll a-b binding protein, chloroplastic Lhcb3 LHCBM5 CHLREDRAFT_184775 CHLRE_03g156900v5 protein protein chlre Chlamydomonas reinhardtii Chlamydomonas smithii MMLSRTVVNVQAKLTKKGGAPKKAAPASAQKKTIREKAGWWSNGGNEKLSAFYGPDRGLWLGPLSGTTPAYLTGEFPGDYGWDSAGLSADPETFKRYRELELIHARWAMLGALGCITPELLAKNGTPIVEPVWFKAGAQIFAEGGLDYLGNPGLVHAQSILATLAVQVILMGAIEGYRVNGGPAGEGLDKLHPGGQFFDPLGLAEDPDAFAELKVKEIKNGRLAMFSMFGFFVQAIVTGKGPLANLDEHLASPFTSNAFTYAQKFTPQ cyf_chlre Cytochrome f petA protein protein chlre Chlamydomonas reinhardtii Chlamydomonas smithii MSNQVFTTLRAATLAVILGMAGGLAVSPAQAYPVFAQQNYANPREANGRIVCANCHLAQKAVEIEVPQAVLPDTVFEAVIELPYDKQVKQVLANGKKGDLNVGMVLILPEGFELAPPDRVPAEIKEKVGNLYYQPYSPEQKNILVVGPVPGKKYSEMVVPILSPDPAKNKNVSYLKYPIYFGGNRGRGQVYPDGKKSNNTIYNASAAGKIVAITALSEKKGGFEVSIEKANGEVVVDKIPAGPDLIVKEGQTVQADQPLTNNPNVGGFGQAETEIVLQNPARIQGLLVFFSFVLLTQVLLVLKKKQFEKVQLAEMNF ucria_chlre Cytochrome b6-f complex iron-sulfur subunit, chloroplastic petC Plastohydroquinone:plastocyanin oxidoreductase iron-sulfur protein Rieske iron-sulfur protein protein protein chlre Chlamydomonas reinhardtii Chlamydomonas smithii MAMLSSRRVAAPAKASAIRRSRVMPVVRAAAASSEVPDMNKRNIMNLILAGGAGLPITTLALGYGAFFVPPSSGGGGGGQAAKDALGNDIKAGEWLKTHLAGDRSLSQGLKGDPTYLIVTADSTIEKYGLNAVCTHLGCVVPWVAAENKFKCPCHGSQYNAEGKVVRGPAPLSLALAHCDVAESGLVTFSTWTETDFRTGLEPWWA cyb6_chlre Cytochrome b6 petB protein protein chlre Chlamydomonas reinhardtii Chlamydomonas smithii MSKVYDWFEERLEIQAIADDITSKYVPPHVNIFYCIGGITFTCFLVQVATGFAMTFYYRPTVAEAFASVQYIMTDVNFGWLIRSIHRWSASMMVLMMVLHVFRVYLTGGFKRPRELTWVTGVIMAVCTVSFGVTGYSLPWDQVGYWAVKIVTGVPDAIPGVGGFIVELLRGGVGVGQATLTRFYSLHTFVLPLLTAVFMLMHFLMIRKQGISGPL psaa-lhcb3-1 3206854 N/A 3206856 unspecified role unspecified role prey prey chlre Chlamydomonas reinhardtii Chlamydomonas smithii N/A 3206857 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chlre Chlamydomonas reinhardtii Chlamydomonas smithii N/A 3206858 unspecified role unspecified role prey prey chlre Chlamydomonas reinhardtii Chlamydomonas smithii N/A 3206859 unspecified role unspecified role prey prey chlre Chlamydomonas reinhardtii Chlamydomonas smithii N/A 3206860 unspecified role unspecified role prey prey chlre Chlamydomonas reinhardtii Chlamydomonas smithii N/A 3206861 unspecified role unspecified role prey prey chlre Chlamydomonas reinhardtii Chlamydomonas smithii N/A 3206862 unspecified role unspecified role prey prey chlre Chlamydomonas reinhardtii Chlamydomonas smithii N/A 3206863 unspecified role unspecified role prey prey chlre Chlamydomonas reinhardtii Chlamydomonas smithii N/A 3206864 unspecified role unspecified role prey prey chlre Chlamydomonas reinhardtii Chlamydomonas smithii N/A 3206865 unspecified role unspecified role prey prey chlre Chlamydomonas reinhardtii Chlamydomonas smithii association association false false false lhcb3-lhcb4-1 3206855 N/A 3206858 unspecified role unspecified role unspecified role unspecified role chlre Chlamydomonas reinhardtii Chlamydomonas smithii N/A 3206860 unspecified role unspecified role unspecified role unspecified role chlre Chlamydomonas reinhardtii Chlamydomonas smithii N/A 3206861 unspecified role unspecified role unspecified role unspecified role chlre Chlamydomonas reinhardtii Chlamydomonas smithii N/A 3206862 unspecified role unspecified role unspecified role unspecified role chlre Chlamydomonas reinhardtii Chlamydomonas smithii N/A 3206856 unspecified role unspecified role unspecified role unspecified role chlre Chlamydomonas reinhardtii Chlamydomonas smithii N/A 3206857 unspecified role unspecified role unspecified role unspecified role chlre Chlamydomonas reinhardtii Chlamydomonas smithii N/A 3206865 unspecified role unspecified role unspecified role unspecified role chlre Chlamydomonas reinhardtii Chlamydomonas smithii N/A 3206863 unspecified role unspecified role unspecified role unspecified role chlre Chlamydomonas reinhardtii Chlamydomonas smithii physical association physical association false false false