DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ chen-2010-6 in vitro In vitro comigration in sds comigration in sds page comigration in sds predetermined predetermined participant predetermined Chen D., Shan J., Zhu WG., Qin J., Gu W. DIP original experiment: DIP-216233X Nature 2010 IMEx Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. Chen D., Shan J., Zhu WG., Qin J., Gu W. Only protein-protein interactions imex curation chen-2010-7 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Chen D., Shan J., Zhu WG., Qin J., Gu W. DIP original experiment: DIP-216220X Nature 2010 IMEx Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. Chen D., Shan J., Zhu WG., Qin J., Gu W. Only protein-protein interactions imex curation chen-2010-8 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Chen D., Shan J., Zhu WG., Qin J., Gu W. DIP original experiment: DIP-216225X Nature 2010 IMEx Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. Chen D., Shan J., Zhu WG., Qin J., Gu W. Only protein-protein interactions imex curation chen-2010-9 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Chen D., Shan J., Zhu WG., Qin J., Gu W. DIP original experiment: DIP-216221X Nature 2010 IMEx Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. Chen D., Shan J., Zhu WG., Qin J., Gu W. Only protein-protein interactions imex curation chen-2010-10 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Chen D., Shan J., Zhu WG., Qin J., Gu W. DIP original experiment: DIP-216229X Nature 2010 IMEx Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. Chen D., Shan J., Zhu WG., Qin J., Gu W. Only protein-protein interactions imex curation chen-2010-11 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Chen D., Shan J., Zhu WG., Qin J., Gu W. DIP original experiment: DIP-216230X Nature 2010 IMEx Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. Chen D., Shan J., Zhu WG., Qin J., Gu W. Only protein-protein interactions imex curation chen-2010-12 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Chen D., Shan J., Zhu WG., Qin J., Gu W. DIP original experiment: DIP-216222X Nature 2010 IMEx Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. Chen D., Shan J., Zhu WG., Qin J., Gu W. Only protein-protein interactions imex curation chen-2010-13 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip ms participant Identification by mass spectrometry ms participant Chen D., Shan J., Zhu WG., Qin J., Gu W. DIP original experiment: DIP-216226X Nature 2010 IMEx Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. Chen D., Shan J., Zhu WG., Qin J., Gu W. Only protein-protein interactions imex curation chen-2010-14 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Chen D., Shan J., Zhu WG., Qin J., Gu W. DIP original experiment: DIP-216231X Nature 2010 IMEx Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. Chen D., Shan J., Zhu WG., Qin J., Gu W. Only protein-protein interactions imex curation chen-2010-15 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Chen D., Shan J., Zhu WG., Qin J., Gu W. DIP original experiment: DIP-216227X Nature 2010 IMEx Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. Chen D., Shan J., Zhu WG., Qin J., Gu W. Only protein-protein interactions imex curation chen-2010-16 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Chen D., Shan J., Zhu WG., Qin J., Gu W. DIP original experiment: DIP-216223X Nature 2010 IMEx Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. Chen D., Shan J., Zhu WG., Qin J., Gu W. Only protein-protein interactions imex curation chen-2010-17 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Chen D., Shan J., Zhu WG., Qin J., Gu W. DIP original experiment: DIP-216232X Nature 2010 IMEx Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. Chen D., Shan J., Zhu WG., Qin J., Gu W. Only protein-protein interactions imex curation chen-2010-18 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Chen D., Shan J., Zhu WG., Qin J., Gu W. DIP original experiment: DIP-216224X Nature 2010 IMEx Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. Chen D., Shan J., Zhu WG., Qin J., Gu W. Only protein-protein interactions imex curation chen-2010-19 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Chen D., Shan J., Zhu WG., Qin J., Gu W. DIP original experiment: DIP-216228X Nature 2010 IMEx Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. Chen D., Shan J., Zhu WG., Qin J., Gu W. Only protein-protein interactions imex curation arf_human Tumor suppressor ARF p14ARF CDKN2A CDKN2 MLM Alternative reading frame Cyclin-dependent kinase inhibitor 2A protein protein human Homo sapiens Human MVRRFLVTLRIRRACGPPRVRVFVVHIPRLTGEWAAPGAPAAVALVLMLLRSQRLGQQPLPRRPGHDDGQRPSGGAAAAPRRGAQLRRPRHSHPTRARRCPGGLPGHAGGAAPGRGAAGRARCLGPSARGPG ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG tripc_human E3 ubiquitin-protein ligase TRIP12 TRIP12 Thyroid receptor-interacting protein 12 KIAA0045 E3 ubiquitin-protein ligase for Arf ULF HECT-type E3 ubiquitin transferase TRIP12 protein protein human Homo sapiens Human MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS This sequence has been withdrawn from Uniprot. 294A7C063A3332DE myc_human Myc proto-oncogene protein BHLHE39 Transcription factor p64 MYC Proto-oncogene c-Myc Class E basic helix-loop-helix protein 39 protein protein human Homo sapiens Human MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAPSEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTELLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPNPARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDSLLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGSPSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNRKCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKATAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA p06748-1 Nucleophosmin NPM1 NPM Nucleolar phosphoprotein B23 Nucleolar protein NO38 Numatrin protein protein human Homo sapiens Human MEDSMDMDMSPLRPQNYLFGCELKADKDYHFKVDNDENEHQLSLRTVSLGAGAKDELHIVEAEAMNYEGSPIKVTLATLKMSVQPTVSLGGFEITPPVVLRLKCGSGPVHISGQHLVAVEEDAESEDEEEEDVKLLSISGKRSAPGGGSKVPQKKVKLAADEDDDDDDEEDDDEDDDDDDFDDEEAEEKAPVKKSIRDTPAKNAQKSNQNGKDSKPSSTPRSKGQESFKKQEKTPKTPKGPSSVEDIKAKMQASIEKGGSLPKVEAKFINYVKNCFRMTDQEAIQDLWQWRKSL cdkn2a-ub-2 3205416 N/A 3205430 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3205431 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false ulf-cdkn2a-1 3205417 N/A 3205430 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3205432 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association false false false ulf-cdkn2a-2 3205418 N/A 3205430 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3205432 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false This tag was used for western blot detection. human Homo sapiens Human physical association physical association false false false cdkn2a-ulf-1 3205419 N/A 3205430 unspecified role unspecified role bait bait human Homo sapiens Human N/A 3205432 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association false false false myc-ulf-1 3205420 N/A 3205433 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3205432 unspecified role unspecified role prey prey 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Region 1-759 Region 1-759 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false ulf-myc-1 3205421 N/A 3205433 unspecified role unspecified role prey prey V5-tag V5-tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human Homo sapiens Human N/A 3205432 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false This tag was used for western blot detection. human Homo sapiens Human physical association physical association false false false cdkn2a-ulf-2 3205422 N/A 3205430 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3205432 unspecified role unspecified role prey prey 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Region 1-759 Region 1-759 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false npm1-ulf-1 3205423 N/A 3205434 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3205432 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association false false false myc-ulf-2 3205424 N/A 3205433 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ULF-binding domain Region 262-439 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3205432 unspecified role unspecified role prey prey 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro direct interaction direct interaction false false false ulf-myc-2 3205425 N/A 3205433 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3205432 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association false false false cdkn2a-ub-3 3205426 N/A 3205430 unspecified role unspecified role bait bait human Homo sapiens Human N/A 3205431 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false human Homo sapiens Human physical association physical association false false false ulf-myc-3 3205427 N/A 3205433 unspecified role unspecified role prey prey ULF-binding domain Region 329-439 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human N/A 3205432 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false This tag was used for western blot detection. human Homo sapiens Human physical association physical association false false false ulf-myc-4 3205428 N/A 3205434 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3205433 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3205432 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false This tag was used for western blot detection. human Homo sapiens Human association association false false false myc-ulf-3 3205429 N/A 3205433 unspecified role unspecified role bait bait human Homo sapiens Human N/A 3205432 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association false false false