DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ spreu-2010-1 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Spreu J., Kuttruff S., Stejfova V., Dennehy KM., Schittek B., Steinle A. DIP original experiment: DIP-216321X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Interaction of C-type lectin-like receptors NKp65 and KACL facilitates dedicated immune recognition of human keratinocytes. Spreu J., Kuttruff S., Stejfova V., Dennehy KM., Schittek B., Steinle A. Only protein-protein interactions imex curation spreu-2010-2 human Homo sapiens Human molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Spreu J., Kuttruff S., Stejfova V., Dennehy KM., Schittek B., Steinle A. DIP original experiment: DIP-216327X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Interaction of C-type lectin-like receptors NKp65 and KACL facilitates dedicated immune recognition of human keratinocytes. Spreu J., Kuttruff S., Stejfova V., Dennehy KM., Schittek B., Steinle A. Only protein-protein interactions imex curation spreu-2010-3 human Homo sapiens Human molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Spreu J., Kuttruff S., Stejfova V., Dennehy KM., Schittek B., Steinle A. DIP original experiment: DIP-216324X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Interaction of C-type lectin-like receptors NKp65 and KACL facilitates dedicated immune recognition of human keratinocytes. Spreu J., Kuttruff S., Stejfova V., Dennehy KM., Schittek B., Steinle A. Only protein-protein interactions imex curation spreu-2010-4 human Homo sapiens Human molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Spreu J., Kuttruff S., Stejfova V., Dennehy KM., Schittek B., Steinle A. DIP original experiment: DIP-216325X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Interaction of C-type lectin-like receptors NKp65 and KACL facilitates dedicated immune recognition of human keratinocytes. Spreu J., Kuttruff S., Stejfova V., Dennehy KM., Schittek B., Steinle A. Only protein-protein interactions imex curation spreu-2010-5 chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey comigration in sds comigration in sds page comigration in sds anti tag western anti tag western blot anti tag western Spreu J., Kuttruff S., Stejfova V., Dennehy KM., Schittek B., Steinle A. DIP original experiment: DIP-216322X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Interaction of C-type lectin-like receptors NKp65 and KACL facilitates dedicated immune recognition of human keratinocytes. Spreu J., Kuttruff S., Stejfova V., Dennehy KM., Schittek B., Steinle A. Only protein-protein interactions imex curation spreu-2010-6 human Homo sapiens Human molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Spreu J., Kuttruff S., Stejfova V., Dennehy KM., Schittek B., Steinle A. DIP original experiment: DIP-216326X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Interaction of C-type lectin-like receptors NKp65 and KACL facilitates dedicated immune recognition of human keratinocytes. Spreu J., Kuttruff S., Stejfova V., Dennehy KM., Schittek B., Steinle A. Only protein-protein interactions imex curation spreu-2010-7 human Homo sapiens Human molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Spreu J., Kuttruff S., Stejfova V., Dennehy KM., Schittek B., Steinle A. DIP original experiment: DIP-216323X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx Interaction of C-type lectin-like receptors NKp65 and KACL facilitates dedicated immune recognition of human keratinocytes. Spreu J., Kuttruff S., Stejfova V., Dennehy KM., Schittek B., Steinle A. Only protein-protein interactions imex curation clc2a_human C-type lectin domain family 2 member A CLEC2A KACL Keratinocyte-associated C-type lectin Proliferation-induced lymphocyte-associated receptor UNQ5792/PRO19597 protein protein human Homo sapiens Human MINPELRDGRADGFIHRIVPKLIQNWKIGLMCFLSIIITTVCIIMIATWSKHAKPVACSGDWLGVRDKCFYFSDDTRNWTASKIFCSLQKAELAQIDTQEDMEFLKRYAGTDMHWIGLSRKQGDSWKWTNGTTFNGWFEIIGNGSFAFLSADGVHSSRGFIDIKWICSKPKYFL klrf2_human Killer cell lectin-like receptor subfamily F member 2 KLRF2 Activating coreceptor NKp65 protein protein human Homo sapiens Human MENEDGYMTLSFKNRCKSKQKSKDFSLYPQYYCLLLIFGCIVILIFIMTGIDLKFWHKKMDFSQNVNVSSLSGHNYLCPNDWLLNEGKCYWFSTSFKTWKESQRDCTQLQAHLLVIQNLDELEFIQNSLKPGHFGWIGLYVTFQGNLWMWIDEHFLVPELFSVIGPTDDRSCAVITGNWVYSEDCSSTFKGICQRDAILTHNGTSGV q9nzs2-1 Killer cell lectin-like receptor subfamily F member 1 KLRF1 CLEC5C ML Activating coreceptor NKp80 C-type lectin domain family 5 member C protein protein human Homo sapiens Human MQDEERYMTLNVQSKKRSSAQTSQLTFKDYSVTLHWYKILLGISGTVNGILTLTLISLILLVSQGVLLKCQKGSCSNATQYEDTGDLKVNNGTRRNISNKDLCASRSADQTVLCQSEWLKYQGKCYWFSNEMKSWSDSYVYCLERKSHLLIIHDQLEMAFIQKNLRQLNYVWIGLNFTSLKMTWTWVDGSPIDSKIFFIKGPAKENSCAAIKESKIFSETCSSVFKWICQY clc2b_human C-type lectin domain family 2 member B CLEC2B AICL Activation-induced C-type lectin C-type lectin superfamily member 2 CLECSF2 IFN-alpha-2b-inducing-related protein 1 IFNRG1 protein protein human Homo sapiens Human MMTKHKKCFIIVGVLITTNIITLIVKLTRDSQSLCPYDWIGFQNKCYYFSKEEGDWNSSKYNCSTQHADLTIIDNIEEMNFLRRYKCSSDHWIGLKMAKNRTGQWVDGATFTKSFGMRGSEGCAYLSDDGAATARCYTERKWICRKRIH nkg2d_human NKG2-D type II integral membrane protein KLRK1 NKG2D NKG2-D-activating NK receptor NK cell receptor D Killer cell lectin-like receptor subfamily K member 1 D12S2489E protein protein human Homo sapiens Human MGWIRGRRSRHSWEMSEFHNYNLDLKKSDFSTRWQKQRCPVVKSKCRENASPFFFCCFIAVAMGIRFIIMVAIWSAVFLNSLFNQEVQIPLTESYCGPCPKNWICYKNNCYQFFDESKNWYESQASCMSQNASLLKVYSKEDQDLLKLVKSYHWMGLVHIPTNGSWQWEDGSILSPNLLTIIEMQKGDCALYASSFKGYIENCSTPNTYICMQRTV clec2a-klrf2-1 3205290 N/A 3205297 unspecified role unspecified role bait bait human Homo sapiens Human N/A 3205298 unspecified role unspecified role prey prey Ectodomain Region 63-207 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human direct interaction direct interaction false false false klrf1-1 3205291 n/a 3205299 unspecified role unspecified role unspecified role unspecified role Ectodomain Region 64-231 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human direct interaction direct interaction false false false clec2b-1 3205292 N/A 3205300 unspecified role unspecified role unspecified role unspecified role Ectodomain Region 26-149 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human direct interaction direct interaction false false false klrk1-1 3205293 N/A 3205301 unspecified role unspecified role unspecified role unspecified role Ectodomain Region 73-216 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human direct interaction direct interaction false false false clec2b-2 3205294 N/A 3205300 unspecified role unspecified role unspecified role unspecified role Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey covalent binding covalent binding false false false klrf2-1 3205295 N/A 3205298 unspecified role unspecified role unspecified role unspecified role Ectodomain Region 63-207 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human direct interaction direct interaction false false false clec2a-1 3205296 N/A 3205297 unspecified role unspecified role unspecified role unspecified role Ectodomain Region 46-174 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human direct interaction direct interaction false false false