DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ bell-2010-2 in vitro In vitro phosphotransferase phosphotransferase assay phosphotransferase weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Bell CH., Porter SL., Strawson A., Stuart DI., Armitage JP. DIP original experiment: DIP-133932X PLoS biology 2010 IMEx Using structural information to change the phosphotransfer specificity of a two-component chemotaxis signalling complex. Bell CH., Porter SL., Strawson A., Stuart DI., Armitage JP. Only protein-protein interactions imex curation bell-2010-3 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Bell CH., Porter SL., Strawson A., Stuart DI., Armitage JP. DIP original experiment: DIP-133930X PLoS biology 2010 IMEx Using structural information to change the phosphotransfer specificity of a two-component chemotaxis signalling complex. Bell CH., Porter SL., Strawson A., Stuart DI., Armitage JP. Only protein-protein interactions imex curation bell-2010-4 in vitro In vitro phosphotransferase phosphotransferase assay phosphotransferase weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Bell CH., Porter SL., Strawson A., Stuart DI., Armitage JP. DIP original experiment: DIP-133934X PLoS biology 2010 IMEx Using structural information to change the phosphotransfer specificity of a two-component chemotaxis signalling complex. Bell CH., Porter SL., Strawson A., Stuart DI., Armitage JP. Only protein-protein interactions imex curation bell-2010-5 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Bell CH., Porter SL., Strawson A., Stuart DI., Armitage JP. DIP original experiment: DIP-133931X PLoS biology 2010 IMEx Using structural information to change the phosphotransfer specificity of a two-component chemotaxis signalling complex. Bell CH., Porter SL., Strawson A., Stuart DI., Armitage JP. Only protein-protein interactions imex curation bell-2010-6 in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Bell CH., Porter SL., Strawson A., Stuart DI., Armitage JP. DIP original experiment: DIP-133935X PLoS biology 2010 IMEx Using structural information to change the phosphotransfer specificity of a two-component chemotaxis signalling complex. Bell CH., Porter SL., Strawson A., Stuart DI., Armitage JP. Only protein-protein interactions imex curation bell-2010-7 in vitro In vitro phosphotransferase phosphotransferase assay phosphotransferase weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Bell CH., Porter SL., Strawson A., Stuart DI., Armitage JP. DIP original experiment: DIP-133933X PLoS biology 2010 IMEx Using structural information to change the phosphotransfer specificity of a two-component chemotaxis signalling complex. Bell CH., Porter SL., Strawson A., Stuart DI., Armitage JP. Only protein-protein interactions imex curation bell-2010-8 in vitro In vitro phosphotransferase phosphotransferase assay phosphotransferase weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Bell CH., Porter SL., Strawson A., Stuart DI., Armitage JP. DIP original experiment: DIP-262155X PLoS biology 2010 IMEx Using structural information to change the phosphotransfer specificity of a two-component chemotaxis signalling complex. Bell CH., Porter SL., Strawson A., Stuart DI., Armitage JP. Only protein-protein interactions imex curation q8kls1_cersp cheY6 EBL86_09020 protein protein rhosh Rhodobacter sphaeroides Rhodopseudomonas sphaeroides MPYNVMIVDDAAMMRLYIASFIKTLPDFKVVAQAANGQEALDKLAAQPNVDLILLDIEMPVMDGMEFLRHAKLKTRAKICMLSSVAVSGSPHAARARELGADGVVAKPSGTVSHDLEEKTGGELARTMRTLMAA q8kls0_cersp cheA3 protein protein rhosh Rhodobacter sphaeroides Rhodopseudomonas sphaeroides MSDAFDEMDEIWALYADDGAQALDAMEASLLALQAGEDAAAHVGPLFRAVHTFKGNSRVLGLSVVESRAHLCEDLIGLVRDAGVPMDGEIVEILLFASDTLRAMLEETAASRADVEGTGSEALMDQLRSKIARCSGAGAATAEVDAATAEERAATAEERAAATEAGAAPTETGAAATSAAEPSEAWGGSPETGAPAAAAEVLPVAGKAFPSVADILGLLDDSALPDTGEAAEVPLEAGGRPAGEGHAPAGPPPEAGPAEAPVPPAAEPARPAARLADDPVYRAIFRDMATAALTRFRAQIDAFATDPGATAQGARAEADGLCHAARQMGLDDWEAALAAFLSDSQGPERLAALVLELEALGDEPAPTPQVEEKPFFAAIAGPLAEIGRLGLRLVTGERPGPAERAAAADPLGQAALAQGYVRVAEAAQALAAAEGAEPFGTARLRLYEELAAVEALMPEAAAAAGVSPVGLLRRLCAAEAAETLAALDATLNAFRSAPLAETFGRFERLMRCVHHACAHHALDAAAQLAMSLVDLFGRAEAKGAVPDAMLVHIARGFIETIELAFEALAQGETPAMEVLDRLFEEAANVCFVSDGLVTAGAIERRLGLPADFHRVLSPESVRAAVEEMEKGQQFYIVRTDINSDDRTAEAFLDWLSGDQARSITNVTVFRGTETLFDFLISSRLGEADLIEALVRMDPSGRRLSLTQALRPRQDEPAPVEAPGEAPLAQGAAITPEMLETIGEISANQAMVQHLLTELAEADLAEATEALVRQAAGDWAAARGPVRALLEAHGARLQEIVQAGTQLTAQLAALQEETVTQRARPASTVLRPLRTFAETLARRRRREVCLTTSGDELALDLALLERLRGFLRGLVALRLDQDRGAPGTLHVSLWRDEERVTVLVQDDGAPEPDSPALADLAAEVGQAGGALRSVPLPGGGMRFHLGLPLSMVVLEGMVVGVDGMRYVLPVESIRTILQADAAAAMTISAAGGQRVLRLSETEIVAIHSLRGHPQEGHRERPWPASRRVYVVLGTGPRSVAIPVDELVGQQLVLLRPLRGVLARLTHLTGLALLAGGDVGMVLSANRICPPLPAEAG q53134_cersp cheY cheY1 protein protein rhosh Rhodobacter sphaeroides Rhodopseudomonas sphaeroides MPLTVLAIDDSRTIRELLREALVQAGFEVHLAIDGLDGLEKLEAAKPHAVITDINMPRMDGFGFIRAVREQPQHSALPIIVLTTESAAELKAKAREAGATAWIVKPFDEAKLVSALRRVAVA q8kls7_cersp Histidine kinase cheA4 protein protein rhosh Rhodobacter sphaeroides Rhodopseudomonas sphaeroides MSAPVEAGPDGGLLRVSSEKIGRLMDLVGELSLSVAETVHSPDLEGLELPAFDGAVHRLRMIVREVQDAATELRLVPVEEVFKRLRRMVRELERQTGKKIEMVFEGAETAIDKLVADQLYDPLLHVVRNSADHGLEPPEERRAAGKPETGRIVLAAAQAGSEVRISVADDGRGLSRPKILKRARERGLFGPDEEPEPATLWKVIFEPGFSTAEAVTNLSGRGVGMDVLNATMTALRGRIGVDSRDGQGSEVSLHIPVSLAFLDCIILRLGARLYALPIDAVSEIVQPKPCDLMGVGAAGGSEMLRLRGHWIPVCRLERFYGEAPSAVPPPAGQVLAVVSTATGLIALPVDEVLDRQQVVMKPLTGRLAQVRASWGCALLGTGEVALVLDGNRLAAGGAA chey_ecoli Chemotaxis protein CheY cheY b1882 JW1871 protein protein ecoli Escherichia coli (strain K12) MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALNKLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAGASGYVVKPFTAATLEEKLNKIFEKLGM chea3-chey6-3 3204534 N/A 3204541 phosphate acceptor phosphate acceptor unspecified role unspecified role Q8KLS1:p.Met13Ser M13S mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q8KLS1:p.[Met13Ser;Leu16Ser;Tyr17Met] M13S/L16S/Y17M mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3204542 phosphate donor phosphate donor unspecified role unspecified role P1-domain Region 1-135 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli phosphotransfer phosphotransfer reaction phosphotransfer false false false chea3-chey6-4 3204535 N/A 3204541 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3204542 unspecified role unspecified role unspecified role unspecified role P1-domain Region 1-135 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false chea3-chey-2 3204536 N/A 3204543 phosphate acceptor phosphate acceptor unspecified role unspecified role ecolx Escherichia coli N/A 3204542 phosphate donor phosphate donor unspecified role unspecified role P1-domain Region 1-135 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli phosphotransfer phosphotransfer reaction phosphotransfer false false false chey6-chea3-1 3204537 N/A 3204541 unspecified role unspecified role bait bait Q8KLS1:p.Met13Ser M13S mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q8KLS1:p.[Met13Ser;Leu16Ser;Tyr17Met] M13S/L16S/Y17M mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3204542 unspecified role unspecified role prey prey P1-domain Region 1-135 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false chea3-chea4-2 3204538 N/A 3204544 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli N/A 3204542 enzyme target enzyme target substrate substrate unspecified role unspecified role P1-domain Region 1-135 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation false false false chea3-chey-3 3204539 N/A 3204545 phosphate acceptor phosphate acceptor unspecified role unspecified role ecolx Escherichia coli N/A 3204542 phosphate donor phosphate donor unspecified role unspecified role P1-domain Region 1-135 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli phosphotransfer phosphotransfer reaction phosphotransfer false false false chea3-chey-4 3204540 N/A 3204545 phosphate acceptor phosphate acceptor unspecified role unspecified role ecolx Escherichia coli N/A 3204542 phosphate donor phosphate donor unspecified role unspecified role P1-domain Region 1-135 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli phosphotransfer phosphotransfer reaction phosphotransfer false false false 1 nodeMerge04 2014-11-28