DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ hatzivassiliou-2010-1 in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Hatzivassiliou G., Song K., Yen I., Brandhuber BJ., Anderson DJ., Alvarado R., Ludlam MJ., Stokoe D., Gloor SL., Vigers G., Morales T., Aliagas I., Liu B., Sideris S., Hoeflich KP., Jaiswal BS., Seshagiri S., Koeppen H., Belvin M., Friedman LS., Malek S. DIP original experiment: DIP-216211X Nature 2010 IMEx RAF inhibitors prime wild-type RAF to activate the MAPK pathway and enhance growth. Hatzivassiliou G., Song K., Yen I., Brandhuber BJ., Anderson DJ., Alvarado R., Ludlam MJ., Stokoe D., Gloor SL., Vigers G., Morales T., Aliagas I., Liu B., Sideris S., Hoeflich KP., Jaiswal BS., Seshagiri S., Koeppen H., Belvin M., Friedman LS., Malek S. Only protein-protein interactions imex curation hatzivassiliou-2010-2 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Hatzivassiliou G., Song K., Yen I., Brandhuber BJ., Anderson DJ., Alvarado R., Ludlam MJ., Stokoe D., Gloor SL., Vigers G., Morales T., Aliagas I., Liu B., Sideris S., Hoeflich KP., Jaiswal BS., Seshagiri S., Koeppen H., Belvin M., Friedman LS., Malek S. DIP original experiment: DIP-216213X Nature 2010 IMEx RAF inhibitors prime wild-type RAF to activate the MAPK pathway and enhance growth. Hatzivassiliou G., Song K., Yen I., Brandhuber BJ., Anderson DJ., Alvarado R., Ludlam MJ., Stokoe D., Gloor SL., Vigers G., Morales T., Aliagas I., Liu B., Sideris S., Hoeflich KP., Jaiswal BS., Seshagiri S., Koeppen H., Belvin M., Friedman LS., Malek S. Only protein-protein interactions imex curation hatzivassiliou-2010-3 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Hatzivassiliou G., Song K., Yen I., Brandhuber BJ., Anderson DJ., Alvarado R., Ludlam MJ., Stokoe D., Gloor SL., Vigers G., Morales T., Aliagas I., Liu B., Sideris S., Hoeflich KP., Jaiswal BS., Seshagiri S., Koeppen H., Belvin M., Friedman LS., Malek S. DIP original experiment: DIP-216206X Nature 2010 IMEx RAF inhibitors prime wild-type RAF to activate the MAPK pathway and enhance growth. Hatzivassiliou G., Song K., Yen I., Brandhuber BJ., Anderson DJ., Alvarado R., Ludlam MJ., Stokoe D., Gloor SL., Vigers G., Morales T., Aliagas I., Liu B., Sideris S., Hoeflich KP., Jaiswal BS., Seshagiri S., Koeppen H., Belvin M., Friedman LS., Malek S. Only protein-protein interactions imex curation hatzivassiliou-2010-4 trini Trichoplusia ni (Cabbage looper) Cabbage looper x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Hatzivassiliou G., Song K., Yen I., Brandhuber BJ., Anderson DJ., Alvarado R., Ludlam MJ., Stokoe D., Gloor SL., Vigers G., Morales T., Aliagas I., Liu B., Sideris S., Hoeflich KP., Jaiswal BS., Seshagiri S., Koeppen H., Belvin M., Friedman LS., Malek S. DIP original experiment: DIP-216209X Nature 2010 IMEx RAF inhibitors prime wild-type RAF to activate the MAPK pathway and enhance growth. Hatzivassiliou G., Song K., Yen I., Brandhuber BJ., Anderson DJ., Alvarado R., Ludlam MJ., Stokoe D., Gloor SL., Vigers G., Morales T., Aliagas I., Liu B., Sideris S., Hoeflich KP., Jaiswal BS., Seshagiri S., Koeppen H., Belvin M., Friedman LS., Malek S. Only protein-protein interactions imex curation hatzivassiliou-2010-5 in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Hatzivassiliou G., Song K., Yen I., Brandhuber BJ., Anderson DJ., Alvarado R., Ludlam MJ., Stokoe D., Gloor SL., Vigers G., Morales T., Aliagas I., Liu B., Sideris S., Hoeflich KP., Jaiswal BS., Seshagiri S., Koeppen H., Belvin M., Friedman LS., Malek S. DIP original experiment: DIP-216212X Nature 2010 IMEx RAF inhibitors prime wild-type RAF to activate the MAPK pathway and enhance growth. Hatzivassiliou G., Song K., Yen I., Brandhuber BJ., Anderson DJ., Alvarado R., Ludlam MJ., Stokoe D., Gloor SL., Vigers G., Morales T., Aliagas I., Liu B., Sideris S., Hoeflich KP., Jaiswal BS., Seshagiri S., Koeppen H., Belvin M., Friedman LS., Malek S. Only protein-protein interactions imex curation hatzivassiliou-2010-6 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Hatzivassiliou G., Song K., Yen I., Brandhuber BJ., Anderson DJ., Alvarado R., Ludlam MJ., Stokoe D., Gloor SL., Vigers G., Morales T., Aliagas I., Liu B., Sideris S., Hoeflich KP., Jaiswal BS., Seshagiri S., Koeppen H., Belvin M., Friedman LS., Malek S. DIP original experiment: DIP-216214X Nature 2010 IMEx RAF inhibitors prime wild-type RAF to activate the MAPK pathway and enhance growth. Hatzivassiliou G., Song K., Yen I., Brandhuber BJ., Anderson DJ., Alvarado R., Ludlam MJ., Stokoe D., Gloor SL., Vigers G., Morales T., Aliagas I., Liu B., Sideris S., Hoeflich KP., Jaiswal BS., Seshagiri S., Koeppen H., Belvin M., Friedman LS., Malek S. Only protein-protein interactions imex curation hatzivassiliou-2010-7 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Hatzivassiliou G., Song K., Yen I., Brandhuber BJ., Anderson DJ., Alvarado R., Ludlam MJ., Stokoe D., Gloor SL., Vigers G., Morales T., Aliagas I., Liu B., Sideris S., Hoeflich KP., Jaiswal BS., Seshagiri S., Koeppen H., Belvin M., Friedman LS., Malek S. DIP original experiment: DIP-216207X Nature 2010 IMEx RAF inhibitors prime wild-type RAF to activate the MAPK pathway and enhance growth. Hatzivassiliou G., Song K., Yen I., Brandhuber BJ., Anderson DJ., Alvarado R., Ludlam MJ., Stokoe D., Gloor SL., Vigers G., Morales T., Aliagas I., Liu B., Sideris S., Hoeflich KP., Jaiswal BS., Seshagiri S., Koeppen H., Belvin M., Friedman LS., Malek S. Only protein-protein interactions imex curation hatzivassiliou-2010-8 in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Hatzivassiliou G., Song K., Yen I., Brandhuber BJ., Anderson DJ., Alvarado R., Ludlam MJ., Stokoe D., Gloor SL., Vigers G., Morales T., Aliagas I., Liu B., Sideris S., Hoeflich KP., Jaiswal BS., Seshagiri S., Koeppen H., Belvin M., Friedman LS., Malek S. DIP original experiment: DIP-216210X Nature 2010 IMEx RAF inhibitors prime wild-type RAF to activate the MAPK pathway and enhance growth. Hatzivassiliou G., Song K., Yen I., Brandhuber BJ., Anderson DJ., Alvarado R., Ludlam MJ., Stokoe D., Gloor SL., Vigers G., Morales T., Aliagas I., Liu B., Sideris S., Hoeflich KP., Jaiswal BS., Seshagiri S., Koeppen H., Belvin M., Friedman LS., Malek S. Only protein-protein interactions imex curation hatzivassiliou-2010-9 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Hatzivassiliou G., Song K., Yen I., Brandhuber BJ., Anderson DJ., Alvarado R., Ludlam MJ., Stokoe D., Gloor SL., Vigers G., Morales T., Aliagas I., Liu B., Sideris S., Hoeflich KP., Jaiswal BS., Seshagiri S., Koeppen H., Belvin M., Friedman LS., Malek S. DIP original experiment: DIP-216208X Nature 2010 IMEx RAF inhibitors prime wild-type RAF to activate the MAPK pathway and enhance growth. Hatzivassiliou G., Song K., Yen I., Brandhuber BJ., Anderson DJ., Alvarado R., Ludlam MJ., Stokoe D., Gloor SL., Vigers G., Morales T., Aliagas I., Liu B., Sideris S., Hoeflich KP., Jaiswal BS., Seshagiri S., Koeppen H., Belvin M., Friedman LS., Malek S. Only protein-protein interactions imex curation braf_human Serine/threonine-protein kinase B-raf BRAF BRAF1 RAFB1 v-Raf murine sarcoma viral oncogene homolog B1 p94 Proto-oncogene B-Raf protein protein human Homo sapiens Human MAALSGGGGGGAEPGQALFNGDMEPEAGAGAGAAASSAADPAIPEEVWNIKQMIKLTQEHIEALLDKFGGEHNPPSIYLEAYEEYTSKLDALQQREQQLLESLGNGTDFSVSSSASMDTVTSSSSSSLSVLPSSLSVFQNPTDVARSNPKSPQKPIVRVFLPNKQRTVVPARCGVTVRDSLKKALMMRGLIPECCAVYRIQDGEKKPIGWDTDISWLTGEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEVPLMCVNYDQLDLLFVSKFFEHHPIPQEEASLAETALTSGSSPSAPASDSIGPQILTSPSPSKSIPIPQPFRPADEDHRNQFGQRDRSSSAPNVHINTIEPVNIDDLIRDQGFRGDGGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSLPKIHRSASEPSLNRAGFQTEDFSLYACASPKTPIQAGGYGAFPVH raf1_human RAF proto-oncogene serine/threonine-protein kinase RAF1 RAF Proto-oncogene c-RAF Raf-1 protein protein human Homo sapiens Human MEHIQGAWKTISNGFGFKDAVFDGSSCISPTIVQQFGYQRRASDDGKLTDPSKTSNTIRVFLPNKQRTVVNVRNGMSLHDCLMKALKVRGLQPECCAVFRLLHEHKGKKARLDWNTDAASLIGEELQVDFLDHVPLTTHNFARKTFLKLAFCDICQKFLLNGFRCQTCGYKFHEHCSTKVPTMCVDWSNIRQLLLFPNSTIGDSGVPALPSLTMRRMRESVSRMPVSSQHRYSTPHAFTFNTSSPSSEGSLSQRQRSTSTPNVHMVSTTLPVDSRMIEDAIRSHSESASPSALSSSPNNLSPTGWSQPKTPVPAQRERAPVSGTQEKNKIRPRGQRDSSYYWEIEASEVMLSTRIGSGSFGTVYKGKWHGDVAVKILKVVDPTPEQFQAFRNEVAVLRKTRHVNILLFMGYMTKDNLAIVTQWCEGSSLYKHLHVQETKFQMFQLIDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKSRWSGSQQVEQPTGSVLWMAPEVIRMQDNNPFSFQSDVYSYGIVLYELMTGELPYSHINNRDQIIFMVGRGYASPDLSKLYKNCPKAMKRLVADCVKKVKEERPLFPQILSSIELLQHSLPKINRSASEPSLHRAAHTEDINACTLTTSPRLPVF araf_human Serine/threonine-protein kinase A-Raf ARAF PKS PKS2 ARAF1 Proto-oncogene A-Raf-1 Proto-oncogene Pks Proto-oncogene A-Raf protein protein human Homo sapiens Human MEPPRGPPANGAEPSRAVGTVKVYLPNKQRTVVTVRDGMSVYDSLDKALKVRGLNQDCCVVYRLIKGRKTVTAWDTAIAPLDGEELIVEVLEDVPLTMHNFVRKTFFSLAFCDFCLKFLFHGFRCQTCGYKFHQHCSSKVPTVCVDMSTNRQQFYHSVQDLSGGSRQHEAPSNRPLNELLTPQGPSPRTQHCDPEHFPFPAPANAPLQRIRSTSTPNVHMVSTTAPMDSNLIQLTGQSFSTDAAGSRGGSDGTPRGSPSPASVSSGRKSPHSKSPAEQRERKSLADDKKKVKNLGYRDSGYYWEVPPSEVQLLKRIGTGSFGTVFRGRWHGDVAVKVLKVSQPTAEQAQAFKNEMQVLRKTRHVNILLFMGFMTRPGFAIITQWCEGSSLYHHLHVADTRFDMVQLIDVARQTAQGMDYLHAKNIIHRDLKSNNIFLHEGLTVKIGDFGLATVKTRWSGAQPLEQPSGSVLWMAAEVIRMQDPNPYSFQSDVYAYGVVLYELMTGSLPYSHIGCRDQIIFMVGRGYLSPDLSKISSNCPKAMRRLLSDCLKFQREERPLFPQILATIELLQRSLPKIERSASEPSLHRTQADELPACLLSAARLVP raf1-braf-5 3202942 N/A 3202951 unspecified role unspecified role prey prey Kinase domain Region 457-717 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 3202952 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction false false false raf1-braf-8 3202943 N/A 3202952 unspecified role unspecified role bait bait Venus-tag Venus-tag fluorescent prot tag fluorescent protein tag fluorescent prot tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3202951 unspecified role unspecified role prey prey mCherry-tag mCherry-tag fluorescent prot tag fluorescent protein tag fluorescent prot tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false araf-braf-1 3202944 N/A 3202953 unspecified role unspecified role bait bait human Homo sapiens Human N/A 3202951 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3202952 unspecified role unspecified role prey prey human Homo sapiens Human association association false false false raf1-1 3202945 N/A 3202952 unspecified role unspecified role unspecified role unspecified role Kinase domain Region 324-618 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction false false false raf1-braf-18 3202946 N/A 3202951 unspecified role unspecified role prey prey Kinase domain Region 457-717 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 3202952 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Kinase domain Region 324-618 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction false false false raf1-braf-20 3202947 N/A 3202952 unspecified role unspecified role bait bait Venus-tag Venus-tag fluorescent prot tag fluorescent protein tag fluorescent prot tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3202951 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association false false false raf1-braf-21 3202948 N/A 3202951 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3202952 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association false false false raf1-raf1-3 3202949 N/A 3202952 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 3202952 unspecified role unspecified role prey prey Kinase domain Region 324-618 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction false false false braf-raf1-23 3202950 N/A 3202951 unspecified role unspecified role bait bait human Homo sapiens Human N/A 3202952 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association false false false