DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ philipp-2010-1 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Philipp S., Puchert M., Adam-Klages S., Tchikov V., Winoto-Morbach S., Mathieu S., Deerberg A., Kolker L., Marchesini N., Kabelitz D., Hannun YA., Schütze S., Adam D. DIP original experiment: DIP-254081X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx The Polycomb group protein EED couples TNF receptor 1 to neutral sphingomyelinase. Philipp S., Puchert M., Adam-Klages S., Tchikov V., Winoto-Morbach S., Mathieu S., Deerberg A., Kolker L., Marchesini N., Kabelitz D., Hannun YA., Schütze S., Adam D. Only protein-protein interactions imex curation philipp-2010-2 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Philipp S., Puchert M., Adam-Klages S., Tchikov V., Winoto-Morbach S., Mathieu S., Deerberg A., Kolker L., Marchesini N., Kabelitz D., Hannun YA., Schütze S., Adam D. DIP original experiment: DIP-254083X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx The Polycomb group protein EED couples TNF receptor 1 to neutral sphingomyelinase. Philipp S., Puchert M., Adam-Klages S., Tchikov V., Winoto-Morbach S., Mathieu S., Deerberg A., Kolker L., Marchesini N., Kabelitz D., Hannun YA., Schütze S., Adam D. Only protein-protein interactions imex curation philipp-2010-3 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Philipp S., Puchert M., Adam-Klages S., Tchikov V., Winoto-Morbach S., Mathieu S., Deerberg A., Kolker L., Marchesini N., Kabelitz D., Hannun YA., Schütze S., Adam D. DIP original experiment: DIP-254087X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx The Polycomb group protein EED couples TNF receptor 1 to neutral sphingomyelinase. Philipp S., Puchert M., Adam-Klages S., Tchikov V., Winoto-Morbach S., Mathieu S., Deerberg A., Kolker L., Marchesini N., Kabelitz D., Hannun YA., Schütze S., Adam D. Only protein-protein interactions imex curation philipp-2010-4 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Philipp S., Puchert M., Adam-Klages S., Tchikov V., Winoto-Morbach S., Mathieu S., Deerberg A., Kolker L., Marchesini N., Kabelitz D., Hannun YA., Schütze S., Adam D. DIP original experiment: DIP-254089X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx The Polycomb group protein EED couples TNF receptor 1 to neutral sphingomyelinase. Philipp S., Puchert M., Adam-Klages S., Tchikov V., Winoto-Morbach S., Mathieu S., Deerberg A., Kolker L., Marchesini N., Kabelitz D., Hannun YA., Schütze S., Adam D. Only protein-protein interactions imex curation philipp-2010-5 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Philipp S., Puchert M., Adam-Klages S., Tchikov V., Winoto-Morbach S., Mathieu S., Deerberg A., Kolker L., Marchesini N., Kabelitz D., Hannun YA., Schütze S., Adam D. DIP original experiment: DIP-254082X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx The Polycomb group protein EED couples TNF receptor 1 to neutral sphingomyelinase. Philipp S., Puchert M., Adam-Klages S., Tchikov V., Winoto-Morbach S., Mathieu S., Deerberg A., Kolker L., Marchesini N., Kabelitz D., Hannun YA., Schütze S., Adam D. Only protein-protein interactions imex curation philipp-2010-6 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Philipp S., Puchert M., Adam-Klages S., Tchikov V., Winoto-Morbach S., Mathieu S., Deerberg A., Kolker L., Marchesini N., Kabelitz D., Hannun YA., Schütze S., Adam D. DIP original experiment: DIP-254084X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx The Polycomb group protein EED couples TNF receptor 1 to neutral sphingomyelinase. Philipp S., Puchert M., Adam-Klages S., Tchikov V., Winoto-Morbach S., Mathieu S., Deerberg A., Kolker L., Marchesini N., Kabelitz D., Hannun YA., Schütze S., Adam D. Only protein-protein interactions imex curation philipp-2010-7 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Philipp S., Puchert M., Adam-Klages S., Tchikov V., Winoto-Morbach S., Mathieu S., Deerberg A., Kolker L., Marchesini N., Kabelitz D., Hannun YA., Schütze S., Adam D. DIP original experiment: DIP-254091X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx The Polycomb group protein EED couples TNF receptor 1 to neutral sphingomyelinase. Philipp S., Puchert M., Adam-Klages S., Tchikov V., Winoto-Morbach S., Mathieu S., Deerberg A., Kolker L., Marchesini N., Kabelitz D., Hannun YA., Schütze S., Adam D. Only protein-protein interactions imex curation philipp-2010-8 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Philipp S., Puchert M., Adam-Klages S., Tchikov V., Winoto-Morbach S., Mathieu S., Deerberg A., Kolker L., Marchesini N., Kabelitz D., Hannun YA., Schütze S., Adam D. DIP original experiment: DIP-254088X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx The Polycomb group protein EED couples TNF receptor 1 to neutral sphingomyelinase. Philipp S., Puchert M., Adam-Klages S., Tchikov V., Winoto-Morbach S., Mathieu S., Deerberg A., Kolker L., Marchesini N., Kabelitz D., Hannun YA., Schütze S., Adam D. Only protein-protein interactions imex curation philipp-2010-9 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Philipp S., Puchert M., Adam-Klages S., Tchikov V., Winoto-Morbach S., Mathieu S., Deerberg A., Kolker L., Marchesini N., Kabelitz D., Hannun YA., Schütze S., Adam D. DIP original experiment: DIP-254086X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx The Polycomb group protein EED couples TNF receptor 1 to neutral sphingomyelinase. Philipp S., Puchert M., Adam-Klages S., Tchikov V., Winoto-Morbach S., Mathieu S., Deerberg A., Kolker L., Marchesini N., Kabelitz D., Hannun YA., Schütze S., Adam D. Only protein-protein interactions imex curation philipp-2010-10 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Philipp S., Puchert M., Adam-Klages S., Tchikov V., Winoto-Morbach S., Mathieu S., Deerberg A., Kolker L., Marchesini N., Kabelitz D., Hannun YA., Schütze S., Adam D. DIP original experiment: DIP-254085X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx The Polycomb group protein EED couples TNF receptor 1 to neutral sphingomyelinase. Philipp S., Puchert M., Adam-Klages S., Tchikov V., Winoto-Morbach S., Mathieu S., Deerberg A., Kolker L., Marchesini N., Kabelitz D., Hannun YA., Schütze S., Adam D. Only protein-protein interactions imex curation philipp-2010-11 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Philipp S., Puchert M., Adam-Klages S., Tchikov V., Winoto-Morbach S., Mathieu S., Deerberg A., Kolker L., Marchesini N., Kabelitz D., Hannun YA., Schütze S., Adam D. DIP original experiment: DIP-254090X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx The Polycomb group protein EED couples TNF receptor 1 to neutral sphingomyelinase. Philipp S., Puchert M., Adam-Klages S., Tchikov V., Winoto-Morbach S., Mathieu S., Deerberg A., Kolker L., Marchesini N., Kabelitz D., Hannun YA., Schütze S., Adam D. Only protein-protein interactions imex curation philipp-2010-12 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Philipp S., Puchert M., Adam-Klages S., Tchikov V., Winoto-Morbach S., Mathieu S., Deerberg A., Kolker L., Marchesini N., Kabelitz D., Hannun YA., Schütze S., Adam D. DIP original experiment: DIP-254092X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx The Polycomb group protein EED couples TNF receptor 1 to neutral sphingomyelinase. Philipp S., Puchert M., Adam-Klages S., Tchikov V., Winoto-Morbach S., Mathieu S., Deerberg A., Kolker L., Marchesini N., Kabelitz D., Hannun YA., Schütze S., Adam D. Only protein-protein interactions imex curation eed_human Polycomb protein EED EED WD protein associating with integrin cytoplasmic tails 1 Embryonic ectoderm development protein protein protein human Homo sapiens Human MSEREVSTAPAGTDMPAAKKQKLSSDENSNPDLSGDENDDAVSIESGTNTERPDTPTNTPNAPGRKSWGKGKWKSKKCKYSFKCVNSLKEDHNQPLFGVQFNWHSKEGDPLVFATVGSNRVTLYECHSQGEIRLLQSYVDADADENFYTCAWTYDSNTSHPLLAVAGSRGIIRIINPITMQCIKHYVGHGNAINELKFHPRDPNLLLSVSKDHALRLWNIQTDTLVAIFGGVEGHRDEVLSADYDLLGEKIMSCGMDHSLKLWRINSKRMMNAIKESYDYNPNKTNRPFISQKIHFPDFSTRDIHRNYVDCVRWLGDLILSKSCENAIVCWKPGKMEDDIDKIKPSESNVTILGRFDYSQCDIWYMRFSMDFWQKMLALGNQVGKLYVWDLEVEDPHKAKCTTLTHHKCGAAIRQTSFSRDSSILIAVCDDASIWRWDRLR nsma2_mouse Sphingomyelin phosphodiesterase 3 Smpd3 Neutral sphingomyelinase 2 Neutral sphingomyelinase II protein protein mouse Mus musculus Mouse MVLYTTPFPNSCLSALHAVSWALIFPCYWLVDRLLASFIPTTYEKRQRADDPCCLQLFCTVLFTPVYLALLVAALPFAFLGFIFWSPLQSARRPYSYSRLEDKNPAGGAALLSEWKGTGAGKSFCFATANVCLLPDSLARLNNVFNTQARAKEIGQRIRNGAARPQIKIYIDSPTNTSISAASFSSLVSPQGGDGSRAVPGSIKRTASVEYKGDGGRHPSDEAANGPASGEQADGSLEDSCIVRIGGEEGGRPQEADDPAAGSQARNGAGGTPKGQTPNHNQRDGDSGSLGSPSASRESLVKARAGQDSGGSGEPGANSKLLYKTSVVKKAAARRRRHPDEAFDHEVSAFFPANLDFLCLQEVFDKRAAAKLKEQLHGYFEYILYDVGVYGCHGCCNFKCLNSGLFFASRYPVMDVAYHCYPNGCSFDALASKGALFLKVQVGSTPQDQRIVGYIACTHLHAPPEDSAVRCEQLDLLQDWLADFRKSTSSTSTANPEELVVFDVICGDLNFDNCSSDDKLEQQHSLFTRYKDPCRLGPGEEKPWAIGTLLDTNGLYDEDVCTPDNLQKVLESEEGRREYLAFPTSKSPGAGQKGRKDLLKGNGRRIDYMLHAEEGLCPDWKAEVEEFSFITQLSGLTDHLPVAMRLMVSAGEEEA nsma2_human Sphingomyelin phosphodiesterase 3 Neutral sphingomyelinase II Neutral sphingomyelinase 2 SMPD3 protein protein human Homo sapiens Human MVLYTTPFPNSCLSALHCVSWALIFPCYWLVDRLAASFIPTTYEKRQRADDPCCLQLLCTALFTPIYLALLVASLPFAFLGFLFWSPLQSARRPYIYSRLEDKGLAGGAALLSEWKGTGPGKSFCFATANVCLLPDSLARVNNLFNTQARAKEIGQRIRNGAARPQIKIYIDSPTNTSISAASFSSLVSPQGGDGVARAVPGSIKRTASVEYKGDGGRHPGDEAANGPASGDPVDSSSPEDACIVRIGGEEGGRPPEADDPVPGGQARNGAGGGPRGQTPNHNQQDGDSGSLGSPSASRESLVKGRAGPDTSASGEPGANSKLLYKASVVKKAAARRRRHPDEAFDHEVSAFFPANLDFLCLQEVFDKRAATKLKEQLHGYFEYILYDVGVYGCQGCCSFKCLNSGLLFASRYPIMDVAYHCYPNKCNDDALASKGALFLKVQVGSTPQDQRIVGYIACTHLHAPQEDSAIRCGQLDLLQDWLADFRKSTSSSSAANPEELVAFDVVCGDFNFDNCSSDDKLEQQHSLFTHYRDPCRLGPGEEKPWAIGTLLDTNGLYDEDVCTPDNLQKVLESEEGRREYLAFPTSKSSGQKGRKELLKGNGRRIDYMLHAEEGLCPDWKAEVEEFSFITQLSGLTDHLPVAMRLMVSSGEEEA rack1_human Receptor of activated protein C kinase 1 Small ribosomal subunit protein RACK1 HLC7 PIG21 RACK1 Cell proliferation-inducing gene 21 protein GNB2L1 Guanine nucleotide-binding protein subunit beta-2-like 1 Guanine nucleotide-binding protein subunit beta-like protein 12.3 Human lung cancer oncogene 7 protein Receptor for activated C kinase protein protein human Homo sapiens Human MTEQMTLRGTLKGHNGWVTQIATTPQFPDMILSASRDKTIIMWKLTRDETNYGIPQRALRGHSHFVSDVVISSDGQFALSGSWDGTLRLWDLTTGTTTRRFVGHTKDVLSVAFSSDNRQIVSGSRDKTIKLWNTLGVCKYTVQDESHSEWVSCVRFSPNSSNPIIVSCGWDKLVKVWNLANCKLKTNHIGHTGYLNTVTVSPDGSLCASGGKDGQAMLWDLNEGKHLYTLDGGDIINALCFSPNRYWLCAATGPSIKIWDLEGKIIVDELKQEVISTSSKAEPPQCTSLAWSADGQTLFAGYTDNLVRVWQVTIGTR tnfa_human Tumor necrosis factor TNF TNFA TNFSF2 TNF-alpha Tumor necrosis factor ligand superfamily member 2 Cachectin protein protein human Homo sapiens Human MSTESMIRDVELAEEALPKKTGGPQGSRRCLFLSLFSFLIVAGATTLFCLLHFGVIGPQREEFPRDLSLISPLAQAVRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL tnr1a_human Tumor necrosis factor receptor superfamily member 1A TNFRSF1A TNFAR TNFR1 p60 Tumor necrosis factor receptor 1 Tumor necrosis factor receptor type I p55 protein protein human Homo sapiens Human MGLSTVPDLLLPLVLLELLVGIYPSGVIGLVPHLGDREKRDSVCPQGKYIHPQNNSICCTKCHKGTYLYNDCPGPGQDTDCRECESGSFTASENHLRHCLSCSKCRKEMGQVEISSCTVDRDTVCGCRKNQYRHYWSENLFQCFNCSLCLNGTVHLSCQEKQNTVCTCHAGFFLRENECVSCSNCKKSLECTKLCLPQIENVKGTEDSGTTVLLPLVIFFGLCLLSLLFIGLMYRYQRWKSKLYSIVCGKSTPEKEGELEGTTTKPLAPNPSFSPTPGFTPTLGFSPVPSSTFTSSSTYTPGDCPNFAAPRREVAPPYQGADPILATALASDPIPNPLQKWEDSAHKPQSLDTDDPATLYAVVENVPPLRWKEFVRRLGLSDHEIDRLELQNGRCLREAQYSMLATWRRRTPRREATLELLGRVLRDMDLLGCLEDIEEALCGPAALPPAPSLLR smpd3-eed-1 3201674 N/A 3201686 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3201687 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast direct interaction direct interaction false false false smpd3-eed-2 3201675 N/A 3201686 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3201688 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association false false false eed-smpd3-1 3201676 N/A 3201686 unspecified role unspecified role bait bait WD repeat I-II-III domain Region 2-233 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3201687 unspecified role unspecified role prey prey C-terminal domain Region 318-655 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast direct interaction direct interaction false false false eed-rack1-1 3201677 N/A 3201689 unspecified role unspecified role prey prey WD repeat V-VII domain Region 204-317 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3201686 unspecified role unspecified role bait bait WD repeat I-II-III domain Region 2-233 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast direct interaction direct interaction false false false eed-smpd3-2 3201678 N/A 3201687 unspecified role unspecified role prey prey C-terminal domain Region 318-655 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3201686 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false smpd3-rack1-1 3201679 N/A 3201689 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3201688 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association false false false eed-smpd3-3 3201680 N/A 3201686 unspecified role unspecified role bait bait human Homo sapiens Human N/A 3201688 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association false false false rack1-eed-1 3201681 N/A 3201689 unspecified role unspecified role bait bait WD repeat IV-VII domain Region 144-317 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3201686 unspecified role unspecified role prey prey WD repeat I-II-III domain Region 2-233 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast direct interaction direct interaction false false false smpd3-eed-3 3201682 N/A 3201686 unspecified role unspecified role prey prey WD repeat I-II-III domain Region 2-233 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3201687 unspecified role unspecified role bait bait C-terminal domain Region 318-655 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast direct interaction direct interaction false false false tnf-eed-1 3201683 N/A 3201690 unspecified role unspecified role bait bait Biotin-tag Biotin-tag biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli N/A 3201689 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3201688 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3201691 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3201686 unspecified role unspecified role prey prey human Homo sapiens Human association association false false false smpd3-eed-4 3201684 N/A 3201687 unspecified role unspecified role bait bait C-terminal domain Region 318-655 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3201686 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false eed-rack1-2 3201685 N/A 3201686 unspecified role unspecified role bait bait human Homo sapiens Human N/A 3201689 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association false false false