DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ smrcka-2010-1 spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm pull down pull down predetermined predetermined participant predetermined Smrcka AV., Kichik N., Tarragó T., Burroughs M., Park MS., Itoga NK., Stern HA., Willardson BM., Giralt E. DIP original experiment: DIP-215883X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx NMR analysis of G-protein betagamma subunit complexes reveals a dynamic G(alpha)-Gbetagamma subunit interface and multiple protein recognition modes. Smrcka AV., Kichik N., Tarragó T., Burroughs M., Park MS., Itoga NK., Stern HA., Willardson BM., Giralt E. Only protein-protein interactions imex curation smrcka-2010-2 spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm dls dynamic light scattering dls predetermined predetermined participant predetermined Smrcka AV., Kichik N., Tarragó T., Burroughs M., Park MS., Itoga NK., Stern HA., Willardson BM., Giralt E. DIP original experiment: DIP-215885X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx NMR analysis of G-protein betagamma subunit complexes reveals a dynamic G(alpha)-Gbetagamma subunit interface and multiple protein recognition modes. Smrcka AV., Kichik N., Tarragó T., Burroughs M., Park MS., Itoga NK., Stern HA., Willardson BM., Giralt E. Only protein-protein interactions imex curation smrcka-2010-3 in vitro In vitro dls dynamic light scattering dls predetermined predetermined participant predetermined Smrcka AV., Kichik N., Tarragó T., Burroughs M., Park MS., Itoga NK., Stern HA., Willardson BM., Giralt E. DIP original experiment: DIP-215887X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx NMR analysis of G-protein betagamma subunit complexes reveals a dynamic G(alpha)-Gbetagamma subunit interface and multiple protein recognition modes. Smrcka AV., Kichik N., Tarragó T., Burroughs M., Park MS., Itoga NK., Stern HA., Willardson BM., Giralt E. Only protein-protein interactions imex curation smrcka-2010-4 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Smrcka AV., Kichik N., Tarragó T., Burroughs M., Park MS., Itoga NK., Stern HA., Willardson BM., Giralt E. DIP original experiment: DIP-215886X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx NMR analysis of G-protein betagamma subunit complexes reveals a dynamic G(alpha)-Gbetagamma subunit interface and multiple protein recognition modes. Smrcka AV., Kichik N., Tarragó T., Burroughs M., Park MS., Itoga NK., Stern HA., Willardson BM., Giralt E. Only protein-protein interactions imex curation smrcka-2010-5 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Smrcka AV., Kichik N., Tarragó T., Burroughs M., Park MS., Itoga NK., Stern HA., Willardson BM., Giralt E. DIP original experiment: DIP-215890X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx NMR analysis of G-protein betagamma subunit complexes reveals a dynamic G(alpha)-Gbetagamma subunit interface and multiple protein recognition modes. Smrcka AV., Kichik N., Tarragó T., Burroughs M., Park MS., Itoga NK., Stern HA., Willardson BM., Giralt E. Only protein-protein interactions imex curation smrcka-2010-6 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Smrcka AV., Kichik N., Tarragó T., Burroughs M., Park MS., Itoga NK., Stern HA., Willardson BM., Giralt E. DIP original experiment: DIP-215892X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx NMR analysis of G-protein betagamma subunit complexes reveals a dynamic G(alpha)-Gbetagamma subunit interface and multiple protein recognition modes. Smrcka AV., Kichik N., Tarragó T., Burroughs M., Park MS., Itoga NK., Stern HA., Willardson BM., Giralt E. Only protein-protein interactions imex curation smrcka-2010-7 spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm ion exchange chrom ion exchange chromatography IEC IEC ion exchange chrom predetermined predetermined participant predetermined Smrcka AV., Kichik N., Tarragó T., Burroughs M., Park MS., Itoga NK., Stern HA., Willardson BM., Giralt E. DIP original experiment: DIP-215891X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx NMR analysis of G-protein betagamma subunit complexes reveals a dynamic G(alpha)-Gbetagamma subunit interface and multiple protein recognition modes. Smrcka AV., Kichik N., Tarragó T., Burroughs M., Park MS., Itoga NK., Stern HA., Willardson BM., Giralt E. Only protein-protein interactions imex curation smrcka-2010-8 spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Smrcka AV., Kichik N., Tarragó T., Burroughs M., Park MS., Itoga NK., Stern HA., Willardson BM., Giralt E. DIP original experiment: DIP-215884X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx NMR analysis of G-protein betagamma subunit complexes reveals a dynamic G(alpha)-Gbetagamma subunit interface and multiple protein recognition modes. Smrcka AV., Kichik N., Tarragó T., Burroughs M., Park MS., Itoga NK., Stern HA., Willardson BM., Giralt E. Only protein-protein interactions imex curation smrcka-2010-9 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Smrcka AV., Kichik N., Tarragó T., Burroughs M., Park MS., Itoga NK., Stern HA., Willardson BM., Giralt E. DIP original experiment: DIP-215888X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx NMR analysis of G-protein betagamma subunit complexes reveals a dynamic G(alpha)-Gbetagamma subunit interface and multiple protein recognition modes. Smrcka AV., Kichik N., Tarragó T., Burroughs M., Park MS., Itoga NK., Stern HA., Willardson BM., Giralt E. Only protein-protein interactions imex curation smrcka-2010-10 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Smrcka AV., Kichik N., Tarragó T., Burroughs M., Park MS., Itoga NK., Stern HA., Willardson BM., Giralt E. DIP original experiment: DIP-215889X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx NMR analysis of G-protein betagamma subunit complexes reveals a dynamic G(alpha)-Gbetagamma subunit interface and multiple protein recognition modes. Smrcka AV., Kichik N., Tarragó T., Burroughs M., Park MS., Itoga NK., Stern HA., Willardson BM., Giralt E. Only protein-protein interactions imex curation gbb1_bovin Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 GNB1 Transducin beta chain 1 protein protein bovin Bos taurus (Bovine) Bovine MSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN gbg2_bovin Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 GNG2 G gamma-I protein protein bovin Bos taurus (Bovine) Bovine MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENPFREKKFFCAIL gnai1_bovin Guanine nucleotide-binding protein G(i) subunit alpha-1 GNAI1 Adenylate cyclase-inhibiting G alpha protein protein protein bovin Bos taurus (Bovine) Bovine MGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEAGYSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMTAELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYPEYAGSNTYEEAAAYIQCQFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli mint scar Ligand peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) SCARFFGTPGCT phos_rat Phosducin Pdc Rpr1 33 kDa phototransducing protein Rod photoreceptor 1 Protein MEKA protein protein rat Rattus norvegicus (Rat) Rat MEEAASQSLEEDFEGQATHTGPKGVINDWRKFKLESEDGDSIPPSKKEILRQMSSPQSRDDKDSKERMSRKMSIQEYELIHQDKEDEGCLRKYRRQCMQDMHQKLSFGPRYGFVYELETGEQFLETIEKEQKVTTIVVNIYEDGVRGCDALNSSLECLAAEYPMVKFCKIRASNTGAGDRFSSDVLPTLLVYKGGELISNFISVAEQFAEDFFAADVESFLNEYGLLPEREIHDLGQTNTEDEDIE mint May participate in the regulation of visual phototransduction or in the integration of photoreceptor metabolism sigk Ligand peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) SIGKAFKILGYPDYD gng2-gnb1-1 3201249 N/A 3201259 unspecified role unspecified role prey prey spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3201260 unspecified role unspecified role bait bait Region 1-67 Region 1-67 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm physical association physical association false false false gnb1-gng2-3 3201250 N/A 3201259 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3201260 unspecified role unspecified role unspecified role unspecified role Region 1-67 Region 1-67 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction false false false gnai1-gnb1-2 3201251 N/A 3201261 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3201259 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3201260 unspecified role unspecified role unspecified role unspecified role Region 1-67 Region 1-67 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm physical association physical association false false false gnai1-gnb1-3 3201252 N/A 3201261 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3201259 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3201260 unspecified role unspecified role unspecified role unspecified role Region 1-67 Region 1-67 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm physical association physical association false false false gnb1-scar-1 3201253 N/A 3201262 unspecified role unspecified role unspecified role unspecified role in vitro In vitro N/A 3201259 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction false false false gnb1-pdc-1 3201254 N/A 3201263 unspecified role unspecified role unspecified role unspecified role N-terminal domain Region 1-107 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false C-terminal domain Region 108-246 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3201259 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction false false false gnb1-gng2-4 3201255 N/A 3201259 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3201260 unspecified role unspecified role unspecified role unspecified role Region 1-67 Region 1-67 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction false false false gnb1-gng2-5 3201256 N/A 3201259 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3201260 unspecified role unspecified role unspecified role unspecified role Region 1-67 Region 1-67 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction false false false gnai1-gnb1-4 3201257 N/A 3201261 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3201259 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction false false false gnb1-sigk-1 3201258 N/A 3201264 unspecified role unspecified role unspecified role unspecified role in vitro In vitro N/A 3201259 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction false false false