DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ pierce-2009-1 in vitro In vitro comigration in sds comigration in sds page comigration in sds predetermined predetermined participant predetermined Pierce NW., Kleiger G., Shan SO., Deshaies RJ. DIP original experiment: DIP-219669X Nature 2009 IMEx Detection of sequential polyubiquitylation on a millisecond timescale. Pierce NW., Kleiger G., Shan SO., Deshaies RJ. Only protein-protein interactions imex curation pierce-2009-2 in vitro In vitro comigration in sds comigration in sds page comigration in sds autoradiography autoradiography Pierce NW., Kleiger G., Shan SO., Deshaies RJ. DIP original experiment: DIP-219670X Nature 2009 IMEx Detection of sequential polyubiquitylation on a millisecond timescale. Pierce NW., Kleiger G., Shan SO., Deshaies RJ. Only protein-protein interactions imex curation pierce-2009-3 in vitro In vitro ms of complexes mass spectrometry studies of complexes ms of complexes predetermined predetermined participant predetermined Pierce NW., Kleiger G., Shan SO., Deshaies RJ. DIP original experiment: DIP-219671X Nature 2009 IMEx Detection of sequential polyubiquitylation on a millisecond timescale. Pierce NW., Kleiger G., Shan SO., Deshaies RJ. Only protein-protein interactions imex curation ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG betacat beta-cat peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) KAWQQQSYLDSGIHSGATTTAPRRASY cyce CYCE peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) KAMLSEQNRASPLPSGLLTPPQSGRRASY ubc3_yeast Ubiquitin-conjugating enzyme E2-34 kDa CDC34 UBC3 DNA6 YDR054C YD9609.08C D4211 Ubiquitin-protein ligase Ubiquitin carrier protein Cell division control protein 34 E3 ubiquitin ligase complex SCF subunit CDC34 E2 ubiquitin-conjugating enzyme 3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSSRKSTASSLLLRQYRELTDPKKAIPSFHIELEDDSNIFTWNIGVMVLNEDSIYHGGFFKAQMRFPEDFPFSPPQFRFTPAIYHPNVYRDGRLCISILHQSGDPMTDEPDAETWSPVQTVESVLISIVSLLEDPNINSPANVDAAVDYRKNPEQYKQRVKMEVERSKQDIPKGFIMPTSESAYISQSKLDEPESNKDMADNFWYDSDLDDDENGSVILQDDDYDDGNNHIPFEDDDVYNYNDNDDDDERIEFEDDDDDDDDSIDNDSVMDRKQPHKAEDESEDVEDVERVSKKI 1CE3E0C3AB1436DC betacat-ub-1 3200121 N/A 3200124 unspecified role unspecified role unspecified role unspecified role N/A 3200125 unspecified role unspecified role unspecified role unspecified role 32P-label 32P-label 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro direct interaction direct interaction false false false cyce-ub-1 3200122 N/A 3200126 unspecified role unspecified role unspecified role unspecified role 32P-label 32P-label 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro N/A 3200124 unspecified role unspecified role unspecified role unspecified role direct interaction direct interaction false false false cdc34-ub-2 3200123 N/A 3200127 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3200124 unspecified role unspecified role unspecified role unspecified role direct interaction direct interaction false false false