DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ lee-2009-38 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Lee SC., Lan W., Buchanan BB., Luan S. DIP original experiment: DIP-134838X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx A protein kinase-phosphatase pair interacts with an ion channel to regulate ABA signaling in plant guard cells. Lee SC., Lan W., Buchanan BB., Luan S. Only protein-protein interactions imex curation lee-2009-39 nicbe Nicotiana benthamiana bifc bimolecular fluorescence complementation bifc predetermined predetermined participant predetermined Lee SC., Lan W., Buchanan BB., Luan S. DIP original experiment: DIP-134836X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx A protein kinase-phosphatase pair interacts with an ion channel to regulate ABA signaling in plant guard cells. Lee SC., Lan W., Buchanan BB., Luan S. Only protein-protein interactions imex curation lee-2009-40 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Lee SC., Lan W., Buchanan BB., Luan S. DIP original experiment: DIP-134841X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx A protein kinase-phosphatase pair interacts with an ion channel to regulate ABA signaling in plant guard cells. Lee SC., Lan W., Buchanan BB., Luan S. Only protein-protein interactions imex curation lee-2009-41 in vitro In vitro phosphatase assay phosphatase assay predetermined predetermined participant predetermined Lee SC., Lan W., Buchanan BB., Luan S. DIP original experiment: DIP-134844X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx A protein kinase-phosphatase pair interacts with an ion channel to regulate ABA signaling in plant guard cells. Lee SC., Lan W., Buchanan BB., Luan S. Only protein-protein interactions imex curation lee-2009-42 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Lee SC., Lan W., Buchanan BB., Luan S. DIP original experiment: DIP-134839X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx A protein kinase-phosphatase pair interacts with an ion channel to regulate ABA signaling in plant guard cells. Lee SC., Lan W., Buchanan BB., Luan S. Only protein-protein interactions imex curation lee-2009-43 nicbe Nicotiana benthamiana bifc bimolecular fluorescence complementation bifc predetermined predetermined participant predetermined Lee SC., Lan W., Buchanan BB., Luan S. DIP original experiment: DIP-134837X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx A protein kinase-phosphatase pair interacts with an ion channel to regulate ABA signaling in plant guard cells. Lee SC., Lan W., Buchanan BB., Luan S. Only protein-protein interactions imex curation lee-2009-44 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Lee SC., Lan W., Buchanan BB., Luan S. DIP original experiment: DIP-134842X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx A protein kinase-phosphatase pair interacts with an ion channel to regulate ABA signaling in plant guard cells. Lee SC., Lan W., Buchanan BB., Luan S. Only protein-protein interactions imex curation lee-2009-45 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Lee SC., Lan W., Buchanan BB., Luan S. DIP original experiment: DIP-134840X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx A protein kinase-phosphatase pair interacts with an ion channel to regulate ABA signaling in plant guard cells. Lee SC., Lan W., Buchanan BB., Luan S. Only protein-protein interactions imex curation lee-2009-46 in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Lee SC., Lan W., Buchanan BB., Luan S. DIP original experiment: DIP-134843X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx A protein kinase-phosphatase pair interacts with an ion channel to regulate ABA signaling in plant guard cells. Lee SC., Lan W., Buchanan BB., Luan S. Only protein-protein interactions imex curation lee-2009-47 in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Lee SC., Lan W., Buchanan BB., Luan S. DIP original experiment: DIP-134845X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx A protein kinase-phosphatase pair interacts with an ion channel to regulate ABA signaling in plant guard cells. Lee SC., Lan W., Buchanan BB., Luan S. Only protein-protein interactions imex curation p2c37_arath Protein phosphatase 2C 37 F24K9.8 At3g11410 PP2CA AHG3 Protein ABA-HYPERSENSITIVE GERMINATION 3 Protein phosphatase 2C A protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAGICCGVVGETEPAAPVDSTSRASLRRRLDLLPSIKIVADSAVAPPLENCRKRQKRETVVLSTLPGNLDLDSNVRSENKKARSAVTNSNSVTEAESFFSDVPKIGTTSVCGRRRDMEDAVSIHPSFLQRNSENHHFYGVFDGHGCSHVAEKCRERLHDIVKKEVEVMASDEWTETMVKSFQKMDKEVSQRECNLVVNGATRSMKNSCRCELQSPQCDAVGSTAVVSVVTPEKIIVSNCGDSRAVLCRNGVAIPLSVDHKPDRPDELIRIQQAGGRVIYWDGARVLGVLAMSRAIGDNYLKPYVIPDPEVTVTDRTDEDECLILASDGLWDVVPNETACGVARMCLRGAGAGDDSDAAHNACSDAALLLTKLALARQSSDNVSVVVVDLRKRRNNQASS srk2e_arath Serine/threonine-protein kinase SRK2E SNRK2.6 Serine/threonine-protein kinase OST1 Protein OPEN STOMATA 1 SNF1-related kinase 2.6 F17I5.140 SRK2E OST1 At4g33950 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDRPAVSGPMDLPIMHDSDRYELVKDIGSGNFGVARLMRDKQSNELVAVKYIERGEKIDENVKREIINHRSLRHPNIVRFKEVILTPTHLAIVMEYASGGELFERICNAGRFSEDEARFFFQQLISGVSYCHAMQVCHRDLKLENTLLDGSPAPRLKICDFGYSKSSVLHSQPKSTVGTPAYIAPEVLLKKEYDGKVADVWSCGVTLYVMLVGAYPFEDPEEPKNFRKTIHRILNVQYAIPDYVHISPECRHLISRIFVADPAKRISIPEIRNHEWFLKNLPADLMNDNTMTTQFDESDQPGQSIEEIMQIIAEATVPPAGTQNLNHYLTGSLDIDDDMEEDLESDLDDLDIDSSGEIVYAM slac1_arath Guard cell S-type anion channel SLAC1 SLAC1 CDI3 OZS1 RCD3 F5O11.23 T12C24.3 At1g12480 Protein CARBON DIOXIDE INSENSITIVE 3 Protein OZONE-SENSITIVE 1 Protein RADICAL-INDUCED CELL DEATH 3 Protein SLOW ANION CHANNEL-ASSOCIATED 1 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MERKQSNAHSTFADINEVEDEAEQELQQQENNNNKRFSGNRGPNRGKQRPFRGFSRQVSLETGFSVLNRESRERDDKKSLPRSGRSFGGFESGGIINGGDGRKTDFSMFRTKSTLSKQKSLLPSIIRERDIENSLRTEDGETKDDSINENVSAGRYFAALRGPELDEVKDNEDILLPKEEQWPFLLRFPIGCFGICLGLSSQAVLWLALAKSPATNFLHITPLINLVVWLFSLVVLVSVSFTYILKCIFYFEAVKREYFHPVRVNFFFAPWVVCMFLAISVPPMFSPNRKYLHPAIWCVFMGPYFFLELKIYGQWLSGGKRRLCKVANPSSHLSVVGNFVGAILASKVGWDEVAKFLWAVGFAHYLVVFVTLYQRLPTSEALPKELHPVYSMFIAAPSAASIAWNTIYGQFDGCSRTCFFIALFLYISLVARINFFTGFKFSVAWWSYTFPMTTASVATIKYAEAVPGYPSRALALTLSFISTAMVCVLFVSTLLHAFVWQTLFPNDLAIAITKRKLTREKKPFKRAYDLKRWTKQALAKKISAEKDFEAEEESHH p2c16_arath Protein phosphatase 2C 16 HAB1 P2C-HA Protein phosphatase 2C HAB1 AtP2C-HA Protein HYPERSENSITIVE TO ABA 1 F28P22.4 At1g72770 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEEMTPAVAMTLSLAANTMCESSPVEITQLKNVTDAADLLSDSENQSFCNGGTECTMEDVSELEEVGEQDLLKTLSDTRSGSSNVFDEDDVLSVVEDNSAVISEGLLVVDAGSELSLSNTAMEIDNGRVLATAIIVGESSIEQVPTAEVLIAGVNQDTNTSEVVIRLPDENSNHLVKGRSVYELDCIPLWGTVSIQGNRSEMEDAFAVSPHFLKLPIKMLMGDHEGMSPSLTHLTGHFFGVYDGHGGHKVADYCRDRLHFALAEEIERIKDELCKRNTGEGRQVQWDKVFTSCFLTVDGEIEGKIGRAVVGSSDKVLEAVASETVGSTAVVALVCSSHIVVSNCGDSRAVLFRGKEAMPLSVDHKPDREDEYARIENAGGKVIQWQGARVFGVLAMSRSIGDRYLKPYVIPEPEVTFMPRSREDECLILASDGLWDVMNNQEVCEIARRRILMWHKKNGAPPLAERGKGIDPACQAAADYLSMLALQKGSKDNISIIVIDLKAQRKFKTRT p2c56_arath Protein phosphatase 2C 56 Protein ABSCISIC ACID-INSENSITIVE 1 ABI1 At4g26080 F20B18.190 Protein phosphatase 2C ABI1 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEEVSPAIAGPFRPFSETQMDFTGIRLGKGYCNNQYSNQDSENGDLMVSLPETSSCSVSGSHGSESRKVLISRINSPNLNMKESAAADIVVVDISAGDEINGSDITSEKKMISRTESRSLFEFKSVPLYGFTSICGRRPEMEDAVSTIPRFLQSSSGSMLDGRFDPQSAAHFFGVYDGHGGSQVANYCRERMHLALAEEIAKEKPMLCDGDTWLEKWKKALFNSFLRVDSEIESVAPETVGSTSVVAVVFPSHIFVANCGDSRAVLCRGKTALPLSVDHKPDREDEAARIEAAGGKVIQWNGARVFGVLAMSRSIGDRYLKPSIIPDPEVTAVKRVKEDDCLILASDGVWDVMTDEEACEMARKRILLWHKKNAVAGDASLLADERRKEGKDPAAMSAAEYLSKLAIQRGSKDNISVVVVDLKPRRKLKSKPLN p2c78_arath Probable protein phosphatase 2C 78 SAG113 HAI1 Protein HIGHLY ABA-INDUCED 1 Protein SENESCENCE-ASSOCIATED GENE 113 MNC17.110 At5g59220 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAEICYENETMMIETTATVVKKATTTTRRRERSSSQAARRRRMEIRRFKFVSGEQEPVFVDGDLQRRRRRESTVAASTSTVFYETAKEVVVLCESLSSTVVALPDPEAYPKYGVASVCGRRREMEDAVAVHPFFSRHQTEYSSTGFHYCGVYDGHGCSHVAMKCRERLHELVREEFEADADWEKSMARSFTRMDMEVVALNADGAAKCRCELQRPDCDAVGSTAVVSVLTPEKIIVANCGDSRAVLCRNGKAIALSSDHKPDRPDELDRIQAAGGRVIYWDGPRVLGVLAMSRAIGDNYLKPYVISRPEVTVTDRANGDDFLILASDGLWDVVSNETACSVVRMCLRGKVNGQVSSSPEREMTGVGAGNVVVGGGDLPDKACEEASLLLTRLALARQSSDNVSVVVVDLRRDT o04719-1 Protein phosphatase 2C 77 PP2C77 ABI2 Protein ABSCISIC ACID-INSENSITIVE 2 Protein phosphatase 2C ABI2 MHM17.19 At5g57050 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDEVSPAVAVPFRPFTDPHAGLRGYCNGESRVTLPESSCSGDGAMKDSSFEINTRQDSLTSSSSAMAGVDISAGDEINGSDEFDPRSMNQSEKKVLSRTESRSLFEFKCVPLYGVTSICGRRPEMEDSVSTIPRFLQVSSSSLLDGRVTNGFNPHLSAHFFGVYDGHGGSQVANYCRERMHLALTEEIVKEKPEFCDGDTWQEKWKKALFNSFMRVDSEIETVAHAPETVGSTSVVAVVFPTHIFVANCGDSRAVLCRGKTPLALSVDHKPDRDDEAARIEAAGGKVIRWNGARVFGVLAMSRSIGDRYLKPSVIPDPEVTSVRRVKEDDCLILASDGLWDVMTNEEVCDLARKRILLWHKKNAMAGEALLPAEKRGEGKDPAAMSAAEYLSKMALQKGSKDNISVVVVDLKGIRKFKSKSLN srk2e-pp2ca-1 3200031 N/A 3200041 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3200042 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast direct interaction direct interaction false false false slac1-srk2e-5 3200032 N/A 3200042 unspecified role unspecified role unspecified role unspecified role N-terminal domain of YFP YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false nicbe Nicotiana benthamiana N/A 3200043 unspecified role unspecified role unspecified role unspecified role C-terminal domain of YFP YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false nicbe Nicotiana benthamiana direct interaction direct interaction false false false srk2e-hab1-2 3200033 N/A 3200044 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3200042 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast direct interaction direct interaction false false false pp2ca-srk2e-1 3200034 N/A 3200041 enzyme enzyme unspecified role unspecified role P49598:p.[Gly139Asp;Gly145Asp] G139D/G145D mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3200042 enzyme target enzyme target substrate substrate unspecified role unspecified role ecolx Escherichia coli dephosphorylation dephosphorylation reaction dephosphorylation false false false srk2e-abi1-3 3200035 N/A 3200045 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3200042 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast direct interaction direct interaction false false false pp2ca-slac1-1 3200036 N/A 3200041 unspecified role unspecified role unspecified role unspecified role N-terminal domain of YFP YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false nicbe Nicotiana benthamiana N/A 3200043 unspecified role unspecified role unspecified role unspecified role C-terminal domain of YFP YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false nicbe Nicotiana benthamiana direct interaction direct interaction false false false srk2e-sag113-2 3200037 N/A 3200046 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3200042 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast direct interaction direct interaction false false false srk2e-abi2-3 3200038 N/A 3200047 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3200042 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast direct interaction direct interaction false false false slac1-srk2e-6 3200039 N/A 3200042 enzyme enzyme unspecified role unspecified role Q940H6:p.Lys50Asn K50N mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3200043 enzyme target enzyme target substrate substrate unspecified role unspecified role ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation false false false srk2e-1 3200040 N/A 3200042 putative self putative self unspecified role unspecified role ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation false false false