DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ oda-2009-1 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Oda S., Schröder M., Khan AR. DIP original experiment: DIP-131971X Structure (London, England : 1993) 2009 IMEx Structural basis for targeting of human RNA helicase DDX3 by poxvirus protein K7. Oda S., Schröder M., Khan AR. Only protein-protein interactions imex curation oda-2009-2 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Oda S., Schröder M., Khan AR. DIP original experiment: DIP-131973X Structure (London, England : 1993) 2009 IMEx Structural basis for targeting of human RNA helicase DDX3 by poxvirus protein K7. Oda S., Schröder M., Khan AR. Only protein-protein interactions imex curation oda-2009-3 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Oda S., Schröder M., Khan AR. DIP original experiment: DIP-131975X Structure (London, England : 1993) 2009 IMEx Structural basis for targeting of human RNA helicase DDX3 by poxvirus protein K7. Oda S., Schröder M., Khan AR. Only protein-protein interactions imex curation oda-2009-4 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Oda S., Schröder M., Khan AR. DIP original experiment: DIP-131972X Structure (London, England : 1993) 2009 IMEx Structural basis for targeting of human RNA helicase DDX3 by poxvirus protein K7. Oda S., Schröder M., Khan AR. Only protein-protein interactions imex curation oda-2009-5 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Oda S., Schröder M., Khan AR. DIP original experiment: DIP-131974X Structure (London, England : 1993) 2009 IMEx Structural basis for targeting of human RNA helicase DDX3 by poxvirus protein K7. Oda S., Schröder M., Khan AR. Only protein-protein interactions imex curation ddx3x_human ATP-dependent RNA helicase DDX3X DDX3X DBX DDX3 DEAD box protein 3, X-chromosomal Helicase-like protein 2 DEAD box, X isoform CAP-Rf protein protein human Homo sapiens Human MSHVAVENALGLDQQFAGLDLNSSDNQSGGSTASKGRYIPPHLRNREATKGFYDKDSSGWSSSKDKDAYSSFGSRSDSRGKSSFFSDRGSGSRGRFDDRGRSDYDGIGSRGDRSGFGKFERGGNSRWCDKSDEDDWSKPLPPSERLEQELFSGGNTGINFEKYDDIPVEATGNNCPPHIESFSDVEMGEIIMGNIELTRYTRPTPVQKHAIPIIKEKRDLMACAQTGSGKTAAFLLPILSQIYSDGPGEALRAMKENGRYGRRKQYPISLVLAPTRELAVQIYEEARKFSYRSRVRPCVVYGGADIGQQIRDLERGCHLLVATPGRLVDMMERGKIGLDFCKYLVLDEADRMLDMGFEPQIRRIVEQDTMPPKGVRHTMMFSATFPKEIQMLARDFLDEYIFLAVGRVGSTSENITQKVVWVEESDKRSFLLDLLNATGKDSLTLVFVETKKGADSLEDFLYHEGYACTSIHGDRSQRDREEALHQFRSGKSPILVATAVAARGLDISNVKHVINFDLPSDIEEYVHRIGRTGRVGNLGLATSFFNERNINITKDLLDLLVEAKQEVPSWLENMAYEHHYKGSSRGRSKSSRFSGGFGARDYRQSSGASSSSFSSSRASSSRSGGGGHGSSRGFGGGGYGGFYNSDGYGGNYNSQGVDWWGN k7_vaccw Protein K7 K7R VACWR039 protein protein vaccw vaccinia virus (stra Vaccinia virus (strain WR) VACV MATKLDYEDAVFYFVDDDKICSRDSIIDLIDEYITWRNHVIVFNKDITSCGRLYKELMKFDDVAIRYYGIDKINEIVEAMSEGDHYINFTKVHDQESLFATIGICAKITEHWGYKKISESRFQSLGNITDLMTDDNINILILFLEKKLN ddx3x-k7_vaccw-1 3198950 N/A 3198955 unspecified role unspecified role bait bait Region 71-90 Region 71-90 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false in vitro In vitro N/A 3198956 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction false false false ddx3x-k7_vaccw-2 3198951 N/A 3198955 unspecified role unspecified role unspecified role unspecified role Region 71-90 Region 71-90 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false O00571:p.[Phe84Ala;Phe85Ala] F84A/F85A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3198956 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false ddx3x-k7_vaccw-3 3198952 N/A 3198955 unspecified role unspecified role unspecified role unspecified role Region 5-167 Region 5-167 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3198956 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false ddx3x-k7_vaccw-4 3198953 N/A 3198955 unspecified role unspecified role unspecified role unspecified role Region 71-90 Region 71-90 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3198956 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false ddx3x-k7_vaccw-5 3198954 N/A 3198955 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false O00571:p.[Phe84Ala;Phe85Ala] F84A/F85A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human N/A 3198956 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false