DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ bergqvist-2009-1 ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Bergqvist S., Alverdi V., Mengel B., Hoffmann A., Ghosh G., Komives EA. DIP original experiment: DIP-134738X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Kinetic enhancement of NF-kappaBxDNA dissociation by IkappaBalpha. Bergqvist S., Alverdi V., Mengel B., Hoffmann A., Ghosh G., Komives EA. Only protein-protein interactions imex curation bergqvist-2009-2 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Bergqvist S., Alverdi V., Mengel B., Hoffmann A., Ghosh G., Komives EA. DIP original experiment: DIP-134740X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Kinetic enhancement of NF-kappaBxDNA dissociation by IkappaBalpha. Bergqvist S., Alverdi V., Mengel B., Hoffmann A., Ghosh G., Komives EA. Only protein-protein interactions imex curation bergqvist-2009-3 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Bergqvist S., Alverdi V., Mengel B., Hoffmann A., Ghosh G., Komives EA. DIP original experiment: DIP-134739X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Kinetic enhancement of NF-kappaBxDNA dissociation by IkappaBalpha. Bergqvist S., Alverdi V., Mengel B., Hoffmann A., Ghosh G., Komives EA. Only protein-protein interactions imex curation bergqvist-2009-4 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Bergqvist S., Alverdi V., Mengel B., Hoffmann A., Ghosh G., Komives EA. DIP original experiment: DIP-134741X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Kinetic enhancement of NF-kappaBxDNA dissociation by IkappaBalpha. Bergqvist S., Alverdi V., Mengel B., Hoffmann A., Ghosh G., Komives EA. Only protein-protein interactions imex curation tf65_mouse Transcription factor p65 Nuclear factor NF-kappa-B p65 subunit Rela Nfkb3 Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 protein protein mouse Mus musculus Mouse MDDLFPLIFPSEPAQASGPYVEIIEQPKQRGMRFRYKCEGRSAGSIPGERSTDTTKTHPTIKINGYTGPGTVRISLVTKDPPHRPHPHELVGKDCRDGYYEADLCPDRSIHSFQNLGIQCVKKRDLEQAISQRIQTNNNPFHVPIEEQRGDYDLNAVRLCFQVTVRDPAGRPLLLTPVLSHPIFDNRAPNTAELKICRVNRNSGSCLGGDEIFLLCDKVQKEDIEVYFTGPGWEARGSFSQADVHRQVAIVFRTPPYADPSLQAPVRVSMQLRRPSDRELSEPMEFQYLPDTDDRHRIEEKRKRTYETFKSIMKKSPFNGPTEPRPPTRRIAVPTRNSTSVPKPAPQPYTFPASLSTINFDEFSPMLLPSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTPGPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSEFQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDEDFSSIADMDFSALLSQISS p25799-1 Nuclear factor NF-kappa-B p105 subunit p105 Nfkb1 DNA-binding factor KBF1 EBP-1 NF-kappa-B1 p84/NF-kappa-B1 p98 Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 protein protein mouse Mus musculus Mouse MADDDPYGTGQMFHLNTALTHSIFNAELYSPEIPLSTDGPYLQILEQPKQRGFRFRYVCEGPSHGGLPGASSEKNKKSYPQVKICNYVGPAKVIVQLVTNGKNIHLHAHSLVGKHCEDGVCTVTAGPKDMVVGFANLGILHVTKKKVFETLEARMTEACIRGYNPGLLVHSDLAYLQAEGGGDRQLTDREKEIIRQAAVQQTKEMDLSVVRLMFTAFLPDSTGSFTRRLEPVVSDAIYDSKAPNASNLKIVRMDRTAGCVTGGEEIYLLCDKVQKDDIQIRFYEEEENGGVWEGFGDFSPTDVHRQFAIVFKTPKYKDVNITKPASVFVQLRRKSDLETSEPKPFLYYPEIKDKEEVQRKRQKLMPNFSDSFGGGSGAGAGGGGMFGSGGGGGSTGSPGPGYGYSNYGFPPYGGITFHPGVTKSNAGVTHGTINTKFKNGPKDCAKSDDEESLTLPEKETEGEGPSLPMACTKTEPIALASTMEDKEQDMGFQDNLFLEKALQLARRHANALFDYAVTGDVKMLLAVQRHLTAVQDENGDSVLHLAIIHLHAQLVRDLLEVTSGLISDDIINMRNDLYQTPLHLAVITKQEDVVEDLLRVGADLSLLDRWGNSVLHLAAKEGHDRILSILLKSRKAAPLIDHPNGEGLNAIHIAVMSNSLPCLLLLVAAGAEVNAQEQKSGRTALHLAVEYDNISLAGCLLLEGDAHVDSTTYDGTTPLHIAAGRGSTRLAALLKAAGADPLVENFEPLYDLDDSWEKAGEDEGVVPGTTPLDMAANWQVFDILNGKPYEPVFTSDDILPQGDMKQLTEDTRLQLCKLLEIPDPDKNWATLAQKLGLGILNNAFRLSPAPSKTLMDNYEVSGGTIKELMEALQQMGYTEAIEVIQAAFRTPATTASSPVTTAQVHCLPLSSSSTRQHIDELRDSDSVCDSGVETSFRKLSFTESLTGDSPLLSLNKMPHGYGQEGPIEGKI nfkb1-rela-5 3198349 N/A 3198353 unspecified role unspecified role prey prey Region 10-321 Region 10-321 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3198354 unspecified role unspecified role bait bait Region 39-350 Region 39-350 polyprotein frag polyprotein fragment polyprotein frag certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli physical association physical association false false false rela-nfkb1-10 3198350 N/A 3198353 unspecified role unspecified role bait bait Region 1-325 Region 1-325 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3198354 unspecified role unspecified role prey prey Region 19-363 Region 19-363 polyprotein frag polyprotein fragment polyprotein frag certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false nfkb1-rela-6 3198351 N/A 3198353 unspecified role unspecified role unspecified role unspecified role Region 10-321 Region 10-321 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3198354 unspecified role unspecified role unspecified role unspecified role Region 39-350 Region 39-350 polyprotein frag polyprotein fragment polyprotein frag certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false rela-rela-10 3198352 N/A 3198353 unspecified role unspecified role prey prey Region 1-325 Region 1-325 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3198353 unspecified role unspecified role bait bait Region 1-325 Region 1-325 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false