DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ miyazono-2009-1 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Miyazono K., Miyakawa T., Sawano Y., Kubota K., Kang HJ., Asano A., Miyauchi Y., Takahashi M., Zhi Y., Fujita Y., Yoshida T., Kodaira KS., Yamaguchi-Shinozaki K., Tanokura M. DIP original experiment: DIP-219614X Nature 2009 IMEx Structural basis of abscisic acid signalling. Miyazono K., Miyakawa T., Sawano Y., Kubota K., Kang HJ., Asano A., Miyauchi Y., Takahashi M., Zhi Y., Fujita Y., Yoshida T., Kodaira KS., Yamaguchi-Shinozaki K., Tanokura M. Only protein-protein interactions imex curation miyazono-2009-2 in vitro In vitro phosphatase assay phosphatase assay predetermined predetermined participant predetermined Miyazono K., Miyakawa T., Sawano Y., Kubota K., Kang HJ., Asano A., Miyauchi Y., Takahashi M., Zhi Y., Fujita Y., Yoshida T., Kodaira KS., Yamaguchi-Shinozaki K., Tanokura M. DIP original experiment: DIP-219615X Nature 2009 IMEx Structural basis of abscisic acid signalling. Miyazono K., Miyakawa T., Sawano Y., Kubota K., Kang HJ., Asano A., Miyauchi Y., Takahashi M., Zhi Y., Fujita Y., Yoshida T., Kodaira KS., Yamaguchi-Shinozaki K., Tanokura M. Only protein-protein interactions imex curation miyazono-2009-3 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Miyazono K., Miyakawa T., Sawano Y., Kubota K., Kang HJ., Asano A., Miyauchi Y., Takahashi M., Zhi Y., Fujita Y., Yoshida T., Kodaira KS., Yamaguchi-Shinozaki K., Tanokura M. DIP original experiment: DIP-219612X Nature 2009 IMEx Structural basis of abscisic acid signalling. Miyazono K., Miyakawa T., Sawano Y., Kubota K., Kang HJ., Asano A., Miyauchi Y., Takahashi M., Zhi Y., Fujita Y., Yoshida T., Kodaira KS., Yamaguchi-Shinozaki K., Tanokura M. Only protein-protein interactions imex curation miyazono-2009-4 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Miyazono K., Miyakawa T., Sawano Y., Kubota K., Kang HJ., Asano A., Miyauchi Y., Takahashi M., Zhi Y., Fujita Y., Yoshida T., Kodaira KS., Yamaguchi-Shinozaki K., Tanokura M. DIP original experiment: DIP-219613X Nature 2009 IMEx Structural basis of abscisic acid signalling. Miyazono K., Miyakawa T., Sawano Y., Kubota K., Kang HJ., Asano A., Miyauchi Y., Takahashi M., Zhi Y., Fujita Y., Yoshida T., Kodaira KS., Yamaguchi-Shinozaki K., Tanokura M. Only protein-protein interactions imex curation pyl1_arath Abscisic acid receptor PYL1 At5g46790 PYL1 RCAR12 PYR1-like protein 1 ABI1-binding protein 6 Regulatory components of ABA receptor 9 MZA15.21 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MANSESSSSPVNEEENSQRISTLHHQTMPSDLTQDEFTQLSQSIAEFHTYQLGNGRCSSLLAQRIHAPPETVWSVVRRFDRPQIYKHFIKSCNVSEDFEMRVGCTRDVNVISGLPANTSRERLDLLDDDRRVTGFSITGGEHRLRNYKSVTTVHRFEKEEEEERIWTVVLESYVVDVPEGNSEEDTRLFADTVIRLNLQKLASITEAMNRNNNNNNSSQVR p2c56_arath Protein phosphatase 2C 56 Protein ABSCISIC ACID-INSENSITIVE 1 ABI1 At4g26080 F20B18.190 Protein phosphatase 2C ABI1 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEEVSPAIAGPFRPFSETQMDFTGIRLGKGYCNNQYSNQDSENGDLMVSLPETSSCSVSGSHGSESRKVLISRINSPNLNMKESAAADIVVVDISAGDEINGSDITSEKKMISRTESRSLFEFKSVPLYGFTSICGRRPEMEDAVSTIPRFLQSSSGSMLDGRFDPQSAAHFFGVYDGHGGSQVANYCRERMHLALAEEIAKEKPMLCDGDTWLEKWKKALFNSFLRVDSEIESVAPETVGSTSVVAVVFPSHIFVANCGDSRAVLCRGKTALPLSVDHKPDREDEAARIEAAGGKVIQWNGARVFGVLAMSRSIGDRYLKPSIIPDPEVTAVKRVKEDDCLILASDGVWDVMTDEEACEMARKRILLWHKKNAVAGDASLLADERRKEGKDPAAMSAAEYLSKLAIQRGSKDNISVVVVDLKPRRKLKSKPLN phosphopeptide Phosphopeptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) RPATVA abi1-pyl1-1 3198105 N/A 3198109 unspecified role unspecified role prey prey Region 8-211 Region 8-211 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Q8VZS8:p.His142Ala H142A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q8VZS8:p.Leu144Ala L144A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q8VZS8:p.Arg143Ala R143A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q8VZS8:p.Phe189Ala F189A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3198110 unspecified role unspecified role bait bait PP2C-domain Region 125-429 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false P49597:p.Ile298Ala I298A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P49597:p.Trp300Ala W300A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P49597:p.Arg304Ala R304A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P49597:p.Glu142Ala E142A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P49597:p.Tyr319Ala Y319A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false abi1-phosphopeptide-1 3198106 N/A 3198111 enzyme target enzyme target substrate substrate unspecified role unspecified role in vitro In vitro N/A 3198110 enzyme enzyme unspecified role unspecified role PP2C-domain Region 125-429 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false P49597:p.Glu142Ala E142A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli dephosphorylation dephosphorylation reaction dephosphorylation false false false abi1-pyl1-2 3198107 N/A 3198109 unspecified role unspecified role unspecified role unspecified role Region 28-210 Region 28-210 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3198110 unspecified role unspecified role unspecified role unspecified role PP2C-domain Region 125-429 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false abi1-pyl1-3 3198108 N/A 3198109 unspecified role unspecified role unspecified role unspecified role Region 28-210 Region 28-210 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3198110 unspecified role unspecified role unspecified role unspecified role PP2C-domain Region 125-429 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false