DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ zhou-2009-8 in vitro In vitro protease assay protease assay anti tag western anti tag western blot anti tag western Zhou R., Cusumano C., Sui D., Garavito RM., Kroos L. DIP original experiment: DIP-134515X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Intramembrane proteolytic cleavage of a membrane-tethered transcription factor by a metalloprotease depends on ATP. Zhou R., Cusumano C., Sui D., Garavito RM., Kroos L. Only protein-protein interactions imex curation zhou-2009-9 ecolx Escherichia coli comigration in gel comigration in gel electrophoresis comigration in gel weight by comassie molecular weight estimation by coomasie staining weight by comassie Zhou R., Cusumano C., Sui D., Garavito RM., Kroos L. DIP original experiment: DIP-134516X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Intramembrane proteolytic cleavage of a membrane-tethered transcription factor by a metalloprotease depends on ATP. Zhou R., Cusumano C., Sui D., Garavito RM., Kroos L. Only protein-protein interactions imex curation zhou-2009-10 ecolx Escherichia coli pull down pull down anti tag western anti tag western blot anti tag western Zhou R., Cusumano C., Sui D., Garavito RM., Kroos L. DIP original experiment: DIP-134517X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Intramembrane proteolytic cleavage of a membrane-tethered transcription factor by a metalloprotease depends on ATP. Zhou R., Cusumano C., Sui D., Garavito RM., Kroos L. Only protein-protein interactions imex curation rpsk_bacsu RNA polymerase sigma-K factor sigK Sigma-27 Stage IV sporulation protein CB cisB spoIIIC spoIVCB BSU25760/BSU26390 protein protein bacsu Bacillus subtilis MVTGVFAALGFVVKELVFLVSYVKNNAFPQPLSSSEEKKYLELMAKGDEHARNMLIEHNLRLVAHIVKKFENTGEDAEDLISIGTIGLIKGIESYSAGKGTKLATYAARCIENEIVITKGGCIHPSLIRFNIYGVRIHNGNFFHDKVNNCFFIFKSMPPLFVMNNEILMHLRALKKTKKDVSLHDPIGQDKEGNEISLIDVLKSENEDVIDTIQLNMELEKVKQYIDILDDREKEVIVGRFGLDLKKEKTQREIAKELGISRSYVSRIEKRALMKMFHEFYRAEKEKRKKAKGK 8665EEAC70892107 sp4fb_bacsu Stage IV sporulation protein FB spoIVFB bofB BSU27970 protein protein bacsu Bacillus subtilis MNKWLDLILKIHVHPFLWIIAALGLLTGHMKALLCLLLIVLIHELGHAALAVFFSWRIKRVFLLPFGGTVEVEEHGNRPLKEEFAVIIAGPLQHIWLQFAAWMLAEVSVIHQHTFELFTFYNLSILFVNLLPIWPLDGGKLLFLLFSKQLPFQKAHRLNLKTSLCFCLLLGCWVLFVIPLQISAWVLFVFLAVSLFEEYRQRHYIHVRFLLERYYGKNRELEKLLPLTVKAEDKVYHVMAEFKRGCKHPIIIEKSGQKLSQLDENEVLHAYFADKRTNSSMEELLLPY 81DB758DC215C916 sigma_k-spoivfb-1 3196793 N/A 3196796 enzyme target enzyme target substrate substrate unspecified role unspecified role His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecolx Escherichia coli N/A 3196797 enzyme enzyme unspecified role unspecified role P26937:p.Glu44Gln E44Q mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli protein cleavage protein cleavage false false false spoivfb-1 3196794 N/A 3196797 unspecified role unspecified role unspecified role unspecified role CBS domain Region 204-288 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false spoivfb-sigma_k-1 3196795 N/A 3196796 unspecified role unspecified role prey prey Region 1-126 Region 1-126 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecolx Escherichia coli N/A 3196797 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false CBS domain Region 204-288 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false