DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ mullins-2009-1 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Mullins FM., Park CY., Dolmetsch RE., Lewis RS. DIP original experiment: DIP-216101X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx STIM1 and calmodulin interact with Orai1 to induce Ca2+-dependent inactivation of CRAC channels. Mullins FM., Park CY., Dolmetsch RE., Lewis RS. Only protein-protein interactions imex curation mullins-2009-2 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Mullins FM., Park CY., Dolmetsch RE., Lewis RS. DIP original experiment: DIP-216103X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx STIM1 and calmodulin interact with Orai1 to induce Ca2+-dependent inactivation of CRAC channels. Mullins FM., Park CY., Dolmetsch RE., Lewis RS. Only protein-protein interactions imex curation mullins-2009-3 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Mullins FM., Park CY., Dolmetsch RE., Lewis RS. DIP original experiment: DIP-216105X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx STIM1 and calmodulin interact with Orai1 to induce Ca2+-dependent inactivation of CRAC channels. Mullins FM., Park CY., Dolmetsch RE., Lewis RS. Only protein-protein interactions imex curation mullins-2009-4 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Mullins FM., Park CY., Dolmetsch RE., Lewis RS. DIP original experiment: DIP-216102X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx STIM1 and calmodulin interact with Orai1 to induce Ca2+-dependent inactivation of CRAC channels. Mullins FM., Park CY., Dolmetsch RE., Lewis RS. Only protein-protein interactions imex curation mullins-2009-5 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Mullins FM., Park CY., Dolmetsch RE., Lewis RS. DIP original experiment: DIP-216104X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx STIM1 and calmodulin interact with Orai1 to induce Ca2+-dependent inactivation of CRAC channels. Mullins FM., Park CY., Dolmetsch RE., Lewis RS. Only protein-protein interactions imex curation crcm1_human Calcium release-activated calcium channel protein 1 ORAI1 CRACM1 TMEM142A Protein orai-1 Transmembrane protein 142A protein protein human Homo sapiens Human MHPEPAPPPSRSSPELPPSGGSTTSGSRRSRRRSGDGEPPGAPPPPPSAVTYPDWIGQSYSEVMSLNEHSMQALSWRKLYLSRAKLKASSRTSALLSGFAMVAMVEVQLDADHDYPPGLLIAFSACTTVLVAVHLFALMISTCILPNIEAVSNVHNLNSVKESPHERMHRHIELAWAFSTVIGTLLFLAEVVLLCWVKFLPLKKQPGQPRPTSKPPASGAAANVSTSGITPGQAAAIASTTIMVPFGLIFIVFAVHFYRSLVSHKTDRQFQELNELAEFARLQDQLDHRGDHPLTPGSHYA calm_rat Calmodulin Calm1 Calm Cam Cam1 Calm2 Cam2 Camb Calm3 Cam3 Camc CaMII protein protein rat Rattus norvegicus (Rat) Rat MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK calm1-orai1-1 3195558 N/A 3195563 unspecified role unspecified role prey prey Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3195564 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false Invalid range Feature range: Line: 154, Character: 10: It is not consistent to have a 'n-terminal' range position with a value different from 1 because 'n-terminal' means the first amino acid of the participant. Actual position : (0-0), It is not consistent to have a 'n-terminal' range position with a value different from 1 because 'n-terminal' means the first amino acid of the participant. Actual position : (0-0) Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false This tag was used for western blot detection. Invalid range Feature range: Line: 198, Character: 10: It is not consistent to have a 'n-terminal' range position with a value different from 1 because 'n-terminal' means the first amino acid of the participant. Actual position : (0-0), It is not consistent to have a 'n-terminal' range position with a value different from 1 because 'n-terminal' means the first amino acid of the participant. Actual position : (0-0) human Homo sapiens Human physical association physical association false false false calm1-orai1-2 3195559 N/A 3195563 unspecified role unspecified role prey prey Region 68-91 Region 68-91 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false YFP-tag YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3195564 unspecified role unspecified role bait bait ecolx Escherichia coli physical association physical association false false false calm1-orai1-3 3195560 N/A 3195563 unspecified role unspecified role prey prey GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N-terminal domain Region 1-91 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Q96D31:p.Ala73Glu A73E mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q96D31:p.Trp76Ala W76A/E/S mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q96D31:p.Tyr80Glu Y80E mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q96D31:p.Trp76Glu mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false Q96D31:p.Trp76Ser mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human N/A 3195564 unspecified role unspecified role bait bait ecolx Escherichia coli physical association physical association false false false calm1-orai1-4 3195561 N/A 3195563 unspecified role unspecified role prey prey YFP-tag YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N-terminal domain Region 1-91 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human N/A 3195564 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false Invalid range Feature range: Line: 1265, Character: 10: It is not consistent to have a 'n-terminal' range position with a value different from 1 because 'n-terminal' means the first amino acid of the participant. Actual position : (0-0), It is not consistent to have a 'n-terminal' range position with a value different from 1 because 'n-terminal' means the first amino acid of the participant. Actual position : (0-0) Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false This tag was used for western blot detection. Invalid range Feature range: Line: 1309, Character: 10: It is not consistent to have a 'n-terminal' range position with a value different from 1 because 'n-terminal' means the first amino acid of the participant. Actual position : (0-0), It is not consistent to have a 'n-terminal' range position with a value different from 1 because 'n-terminal' means the first amino acid of the participant. Actual position : (0-0) human Homo sapiens Human physical association physical association false false false orai1-calm1-1 3195562 N/A 3195563 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 48-91 Region 48-91 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3195564 unspecified role unspecified role prey prey HRP-tag HRP-tag hrp tag horseradish peroxidase tag hrp tag c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal false Out of date range Feature range: Line: 1666, Character: 10: It is not consistent to have a 'c-terminal' range position with a value different from the sequence length because 'c-terminal' means the last amino acid of the participant.However, we can accept a position null or 0 if the participant sequence is not known. Actual position : (0-0), sequence length: 149, It is not consistent to have a 'c-terminal' range position with a value different from the sequence length because 'c-terminal' means the last amino acid of the participant.However, we can accept a position null or 0 if the participant sequence is not known. Actual position : (0-0), sequence length: 149 ecolx Escherichia coli physical association physical association false false false