DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dias-2009-4 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Dias SM., Wilson KF., Rojas KS., Ambrosio AL., Cerione RA. DIP original experiment: DIP-133253X Nature structural & molecular biology 2009 IMEx The molecular basis for the regulation of the cap-binding complex by the importins. Dias SM., Wilson KF., Rojas KS., Ambrosio AL., Cerione RA. Only protein-protein interactions imex curation dias-2009-5 spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Dias SM., Wilson KF., Rojas KS., Ambrosio AL., Cerione RA. DIP original experiment: DIP-133255X Nature structural & molecular biology 2009 IMEx The molecular basis for the regulation of the cap-binding complex by the importins. Dias SM., Wilson KF., Rojas KS., Ambrosio AL., Cerione RA. Only protein-protein interactions imex curation dias-2009-6 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Dias SM., Wilson KF., Rojas KS., Ambrosio AL., Cerione RA. DIP original experiment: DIP-133257X Nature structural & molecular biology 2009 IMEx The molecular basis for the regulation of the cap-binding complex by the importins. Dias SM., Wilson KF., Rojas KS., Ambrosio AL., Cerione RA. Only protein-protein interactions imex curation dias-2009-7 in vitro In vitro saxs x ray scattering saxs predetermined predetermined participant predetermined Dias SM., Wilson KF., Rojas KS., Ambrosio AL., Cerione RA. DIP original experiment: DIP-133260X Nature structural & molecular biology 2009 IMEx The molecular basis for the regulation of the cap-binding complex by the importins. Dias SM., Wilson KF., Rojas KS., Ambrosio AL., Cerione RA. Only protein-protein interactions imex curation dias-2009-8 in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Dias SM., Wilson KF., Rojas KS., Ambrosio AL., Cerione RA. DIP original experiment: DIP-133259X Nature structural & molecular biology 2009 IMEx The molecular basis for the regulation of the cap-binding complex by the importins. Dias SM., Wilson KF., Rojas KS., Ambrosio AL., Cerione RA. Only protein-protein interactions imex curation dias-2009-9 in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel weight by comassie molecular weight estimation by coomasie staining weight by comassie Dias SM., Wilson KF., Rojas KS., Ambrosio AL., Cerione RA. DIP original experiment: DIP-133262X Nature structural & molecular biology 2009 IMEx The molecular basis for the regulation of the cap-binding complex by the importins. Dias SM., Wilson KF., Rojas KS., Ambrosio AL., Cerione RA. Only protein-protein interactions imex curation dias-2009-10 spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel weight by comassie molecular weight estimation by coomasie staining weight by comassie Dias SM., Wilson KF., Rojas KS., Ambrosio AL., Cerione RA. DIP original experiment: DIP-133264X Nature structural & molecular biology 2009 IMEx The molecular basis for the regulation of the cap-binding complex by the importins. Dias SM., Wilson KF., Rojas KS., Ambrosio AL., Cerione RA. Only protein-protein interactions imex curation dias-2009-11 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Dias SM., Wilson KF., Rojas KS., Ambrosio AL., Cerione RA. DIP original experiment: DIP-133256X Nature structural & molecular biology 2009 IMEx The molecular basis for the regulation of the cap-binding complex by the importins. Dias SM., Wilson KF., Rojas KS., Ambrosio AL., Cerione RA. Only protein-protein interactions imex curation dias-2009-12 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Dias SM., Wilson KF., Rojas KS., Ambrosio AL., Cerione RA. DIP original experiment: DIP-133254X Nature structural & molecular biology 2009 IMEx The molecular basis for the regulation of the cap-binding complex by the importins. Dias SM., Wilson KF., Rojas KS., Ambrosio AL., Cerione RA. Only protein-protein interactions imex curation dias-2009-13 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Dias SM., Wilson KF., Rojas KS., Ambrosio AL., Cerione RA. DIP original experiment: DIP-133258X Nature structural & molecular biology 2009 IMEx The molecular basis for the regulation of the cap-binding complex by the importins. Dias SM., Wilson KF., Rojas KS., Ambrosio AL., Cerione RA. Only protein-protein interactions imex curation dias-2009-14 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Dias SM., Wilson KF., Rojas KS., Ambrosio AL., Cerione RA. DIP original experiment: DIP-133261X Nature structural & molecular biology 2009 IMEx The molecular basis for the regulation of the cap-binding complex by the importins. Dias SM., Wilson KF., Rojas KS., Ambrosio AL., Cerione RA. Only protein-protein interactions imex curation dias-2009-15 in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel weight by comassie molecular weight estimation by coomasie staining weight by comassie Dias SM., Wilson KF., Rojas KS., Ambrosio AL., Cerione RA. DIP original experiment: DIP-133263X Nature structural & molecular biology 2009 IMEx The molecular basis for the regulation of the cap-binding complex by the importins. Dias SM., Wilson KF., Rojas KS., Ambrosio AL., Cerione RA. Only protein-protein interactions imex curation ima1_human Importin subunit alpha-1 KPNA2 RCH1 SRP1 Karyopherin subunit alpha-2 SRP1-alpha RAG cohort protein 1 protein protein human Homo sapiens Human MSTNENANTPAARLHRFKNKGKDSTEMRRRRIEVNVELRKAKKDDQMLKRRNVSSFPDDATSPLQENRNNQGTVNWSVDDIVKGINSSNVENQLQATQAARKLLSREKQPPIDNIIRAGLIPKFVSFLGRTDCSPIQFESAWALTNIASGTSEQTKAVVDGGAIPAFISLLASPHAHISEQAVWALGNIAGDGSVFRDLVIKYGAVDPLLALLAVPDMSSLACGYLRNLTWTLSNLCRNKNPAPPIDAVEQILPTLVRLLHHDDPEVLADTCWAISYLTDGPNERIGMVVKTGVVPQLVKLLGASELPIVTPALRAIGNIVTGTDEQTQVVIDAGALAVFPSLLTNPKTNIQKEATWTMSNITAGRQDQIQQVVNHGLVPFLVSVLSKADFKTQKEAVWAVTNYTSGGTVEQIVYLVHCGIIEPLMNLLTAKDTKIILVILDAISNIFQAAEKLGETEKLSIMIEECGGLDKIEALQNHENESVYKASLSLIEKYFSVEEEEDQNVVPETTSEGYTFQVQDGAPGTFNF p52298-1 Nuclear cap-binding protein subunit 2 NCBP2 CBP20 20 kDa nuclear cap-binding protein Cell proliferation-inducing gene 55 protein NCBP 20 kDa subunit NCBP-interacting protein 1 PIG55 protein protein human Homo sapiens Human MSGGLLKALRSDSYVELSQYRDQHFRGDNEEQEKLLKKSCTLYVGNLSFYTTEEQIYELFSKSGDIKKIIMGLDKMKKTACGFCFVEYYSRADAENAMRYINGTRLDDRIIRTDWDAGFKEGRQYGRGRSGGQVRDEYRQDYDAGRGGYGKLAQNQ ncbp1_human Nuclear cap-binding protein subunit 1 NCBP1 CBP80 NCBP 80 kDa nuclear cap-binding protein protein protein human Homo sapiens Human MSRRRHSDENDGGQPHKRRKTSDANETEDHLESLICKVGEKSACSLESNLEGLAGVLEADLPNYKSKILRLLCTVARLLPEKLTIYTTLVGLLNARNYNFGGEFVEAMIRQLKESLKANNYNEAVYLVRFLSDLVNCHVIAAPSMVAMFENFVSVTQEEDVPQVRRDWYVYAFLSSLPWVGKELYEKKDAEMDRIFANTESYLKRRQKTHVPMLQVWTADKPHPQEEYLDCLWAQIQKLKKDRWQERHILRPYLAFDSILCEALQHNLPPFTPPPHTEDSVYPMPRVIFRMFDYTDDPEGPVMPGSHSVERFVIEENLHCIIKSHWKERKTCAAQLVSYPGKNKIPLNYHIVEVIFAELFQLPAPPHIDVMYTTLLIELCKLQPGSLPQVLAQATEMLYMRLDTMNTTCVDRFINWFSHHLSNFQFRWSWEDWSDCLSQDPESPKPKFVREVLEKCMRLSYHQRILDIVPPTFSALCPANPTCIYKYGDESSNSLPGHSVALCLAVAFKSKATNDEIFSILKDVPNPNQDDDDDEGFSFNPLKIEVFVQTLLHLAAKSFSHSFSALAKFHEVFKTLAESDEGKLHVLRVMFEVWRNHPQMIAVLVDKMIRTQIVDCAAVANWIFSSELSRDFTRLFVWEILHSTIRKMNKHVLKIQKELEEAKEKLARQHKRRSDDDDRSSDRKDGVLEEQIERLQEKVESAQSEQKNLFLVIFQRFIMILTEHLVRCETDGTSVLTPWYKNCIERLQQIFLQHHQIIQQYMVTLENLLFTAELDPHILAVFQQFCALQA q14974-1 Importin subunit beta-1 Importin-90 Karyopherin subunit beta-1 Nuclear factor p97 Pore targeting complex 97 kDa subunit KPNB1 NTF97 protein protein human Homo sapiens Human MELITILEKTVSPDRLELEAAQKFLERAAVENLPTFLVELSRVLANPGNSQVARVAAGLQIKNSLTSKDPDIKAQYQQRWLAIDANARREVKNYVLQTLGTETYRPSSASQCVAGIACAEIPVNQWPELIPQLVANVTNPNSTEHMKESTLEAIGYICQDIDPEQLQDKSNEILTAIIQGMRKEEPSNNVKLAATNALLNSLEFTKANFDKESERHFIMQVVCEATQCPDTRVRVAALQNLVKIMSLYYQYMETYMGPALFAITIEAMKSDIDEVALQGIEFWSNVCDEEMDLAIEASEAAEQGRPPEHTSKFYAKGALQYLVPILTQTLTKQDENDDDDDWNPCKAAGVCLMLLATCCEDDIVPHVLPFIKEHIKNPDWRYRDAAVMAFGCILEGPEPSQLKPLVIQAMPTLIELMKDPSVVVRDTAAWTVGRICELLPEAAINDVYLAPLLQCLIEGLSAEPRVASNVCWAFSSLAEAAYEAADVADDQEEPATYCLSSSFELIVQKLLETTDRPDGHQNNLRSSAYESLMEIVKNSAKDCYPAVQKTTLVIMERLQQVLQMESHIQSTSDRIQFNDLQSLLCATLQNVLRKVQHQDALQISDVVMASLLRMFQSTAGSGGVQEDALMAVSTLVEVLGGEFLKYMEAFKPFLGIGLKNYAEYQVCLAAVGLVGDLCRALQSNIIPFCDEVMQLLLENLGNENVHRSVKPQILSVFGDIALAIGGEFKKYLEVVLNTLQQASQAQVDKSDYDMVDYLNELRESCLEAYTGIVQGLKGDQENVHPDVMLVQPRVEFILSFIDHIAGDEDHTDGVVACAAGLIGDLCTAFGKDVLKLVEARPMIHELLTEGRRSKTNKAKTLATWATKELRKLKNQA kpna2-ncbp1-1 3195129 N/A 3195141 unspecified role unspecified role unspecified role unspecified role Region 70-529 Region 70-529 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3195142 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3195143 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm physical association physical association false false false ncbp1-ncbp2-7 3195130 N/A 3195142 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3195143 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction false false false ncbp2-kpna2-1 3195131 N/A 3195144 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3195141 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3195142 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3195143 unspecified role unspecified role prey prey spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm association association false false false kpna2-kpnb1-4 3195132 N/A 3195144 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3195141 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3195142 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3195143 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm physical association physical association false false false kpna2-kpnb1-5 3195133 N/A 3195144 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3195141 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3195142 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3195143 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm physical association physical association false false false kpnb1-ncbp1-1 3195134 N/A 3195144 unspecified role unspecified role unspecified role unspecified role Region 1-445 Region 1-445 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3195142 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3195143 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm physical association physical association false false false ncbp1-ncbp2-8 3195135 N/A 3195142 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3195143 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction false false false ncbp2-ncbp1-1 3195136 N/A 3195142 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 15-125 Region 15-125 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3195143 unspecified role unspecified role prey prey spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction false false false kpna2-ncbp1-2 3195137 N/A 3195141 unspecified role unspecified role unspecified role unspecified role Region 70-529 Region 70-529 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3195142 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3195143 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction false false false kpna2-kpnb1-6 3195138 N/A 3195144 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3195141 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3195142 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3195143 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm physical association physical association false false false ncbp1-ncbp2-9 3195139 N/A 3195142 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3195143 unspecified role unspecified role bait bait V5-tag V5-tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human Homo sapiens Human physical association physical association false false false kpnb1-ncbp2-1 3195140 N/A 3195144 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3195142 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false