DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ lee-2009-27 azovi Azotobacter vinelandii pull down pull down edman degradation edman degradation Lee CC., Hu Y., Ribbe MW. DIP original experiment: DIP-134286X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Unique features of the nitrogenase VFe protein from Azotobacter vinelandii. Lee CC., Hu Y., Ribbe MW. Only protein-protein interactions imex curation lee-2009-28 azovi Azotobacter vinelandii molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Lee CC., Hu Y., Ribbe MW. DIP original experiment: DIP-134288X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Unique features of the nitrogenase VFe protein from Azotobacter vinelandii. Lee CC., Hu Y., Ribbe MW. Only protein-protein interactions imex curation lee-2009-29 azovi Azotobacter vinelandii comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel predetermined predetermined participant predetermined Lee CC., Hu Y., Ribbe MW. DIP original experiment: DIP-134287X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Unique features of the nitrogenase VFe protein from Azotobacter vinelandii. Lee CC., Hu Y., Ribbe MW. Only protein-protein interactions imex curation vnfg_azovi Nitrogenase vanadium-iron protein delta chain vnfG Dinitrogenase 2 subunit delta Nitrogenase component I protein protein azovi Azotobacter vinelandii MSQSHLDDLFAYVEERCLWQFFSRTWDREENIEGVLNQVGRLLTGQEPLRGTPQERLFYADALAMANDVRERFPWASQVNKEEIEFLLDGLKSRLVDVTITRSTNRELNHHLY vnfk_azovi Nitrogenase vanadium-iron protein beta chain vnfK Dinitrogenase 2 subunit beta Nitrogenase component I protein protein azovi Azotobacter vinelandii MSNCELTVLKPAEVKLSPRDREGIINPMYDCQPAGAQYAGIGIKDCIPLVHGGQGCTMFVRLLFAQHFKENFDVASTSLHEESAVFGGAKRVEEGVLVLARRYPNLRVIPIITTCSTEVIGDDIEGSIRVCNRALEAEFPDRKIYLAPVHTPSFKGSHVTGYAECVKSVFKTITDAHGKGQPSGKLNVFPGWVNPGDVVLLKRYFKEMDVEANIYMDTEDFDSPMLPNKSIETHGRTTVEDIADSANALATLSLARYEGNTTGELLQKTFAVPNALVNTPYGIKNTDDMLRKIAEVTGKEIPESLVRERGIALDALADLAHMFFANKKVAIFGHPDLVLGLAQFCMEVELEPVLLLIGDDQGNKYKKDPRIEELKNTAHFDIEIVHNADLWELEKRINAGLQLDLIMGHSKGRYVAIEANIPMVRVGFPTFDRAGLYRKPSIGYQGAMELGEMIANAMFAHMEYTRNKEWILNTW vnfd_azovi Nitrogenase vanadium-iron protein alpha chain vnfD Dinitrogenase 2 subunit alpha Nitrogenase component I protein protein azovi Azotobacter vinelandii MPMVLLECDKDIPERQKHIYLKAPNEDTREFLPIANAATIPGTLSERGCAFCGAKLVIGGVLKDTIQMIHGPLGCAYDTWHTKRYPTDNGHFNMKYVWSTDMKESHVVFGGEKRLEKSMHEAFDEMPDIKRMIVYTTCPTALIGDDIKAVAKKVMKDRPDVDVFTVECPGFSGVSQSKGHHVLNIGWINEKVETMEKEITSEYTMNFIGDFNIQGDTQLLQTYWDRLGIQVVAHFTGNGTYDDLRCMHQAQLNVVNCARSSGYIANELKKRYGIPRLDIDSWGFNYMAEGIRKICAFFGIEEKGEELIAEEYAKWKPKLDWYKERLQGKKMAIWTGGPRLWHWTKSVEDDLGVQVVAMSSKFGHEEDFEKVIARGKEGTYYIDDGNELEFFEIIDLVKPDVIFTGPRVGELVKKLHIPYVNGHGYHNGPYMGFEGFVNLARDMYNAVHNPLRHLAAVDIRDKSQTTPVIVRGAA vnfk-vnfd-1 3191541 N/A 3191544 unspecified role unspecified role prey prey azovi Azotobacter vinelandii N/A 3191545 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false azovi Azotobacter vinelandii N/A 3191546 unspecified role unspecified role prey prey azovi Azotobacter vinelandii association association false false false vnfd-vnfg-1 3191542 N/A 3191544 unspecified role unspecified role unspecified role unspecified role azovi Azotobacter vinelandii N/A 3191545 unspecified role unspecified role unspecified role unspecified role azovi Azotobacter vinelandii N/A 3191546 unspecified role unspecified role unspecified role unspecified role azovi Azotobacter vinelandii physical association physical association false false false vnfd-vnfg-2 3191543 N/A 3191544 unspecified role unspecified role unspecified role unspecified role azovi Azotobacter vinelandii N/A 3191545 unspecified role unspecified role unspecified role unspecified role azovi Azotobacter vinelandii N/A 3191546 unspecified role unspecified role unspecified role unspecified role azovi Azotobacter vinelandii physical association physical association false false false