DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ wang-2009-17 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Wang GG., Song J., Wang Z., Dormann HL., Casadio F., Li H., Luo JL., Patel DJ., Allis CD. DIP original experiment: DIP-249904X Nature 2009 IMEx Haematopoietic malignancies caused by dysregulation of a chromatin-binding PHD finger. Wang GG., Song J., Wang Z., Dormann HL., Casadio F., Li H., Luo JL., Patel DJ., Allis CD. Only protein-protein interactions imex curation wang-2009-18 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Wang GG., Song J., Wang Z., Dormann HL., Casadio F., Li H., Luo JL., Patel DJ., Allis CD. DIP original experiment: DIP-249902X Nature 2009 IMEx Haematopoietic malignancies caused by dysregulation of a chromatin-binding PHD finger. Wang GG., Song J., Wang Z., Dormann HL., Casadio F., Li H., Luo JL., Patel DJ., Allis CD. Only protein-protein interactions imex curation wang-2009-19 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Wang GG., Song J., Wang Z., Dormann HL., Casadio F., Li H., Luo JL., Patel DJ., Allis CD. DIP original experiment: DIP-249906X Nature 2009 IMEx Haematopoietic malignancies caused by dysregulation of a chromatin-binding PHD finger. Wang GG., Song J., Wang Z., Dormann HL., Casadio F., Li H., Luo JL., Patel DJ., Allis CD. Only protein-protein interactions imex curation wang-2009-20 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Wang GG., Song J., Wang Z., Dormann HL., Casadio F., Li H., Luo JL., Patel DJ., Allis CD. DIP original experiment: DIP-249905X Nature 2009 IMEx Haematopoietic malignancies caused by dysregulation of a chromatin-binding PHD finger. Wang GG., Song J., Wang Z., Dormann HL., Casadio F., Li H., Luo JL., Patel DJ., Allis CD. Only protein-protein interactions imex curation wang-2009-21 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Wang GG., Song J., Wang Z., Dormann HL., Casadio F., Li H., Luo JL., Patel DJ., Allis CD. DIP original experiment: DIP-249903X Nature 2009 IMEx Haematopoietic malignancies caused by dysregulation of a chromatin-binding PHD finger. Wang GG., Song J., Wang Z., Dormann HL., Casadio F., Li H., Luo JL., Patel DJ., Allis CD. Only protein-protein interactions imex curation h3tail H3 tail peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) ARTKQTARKSTGGKAPRKQL kdm5a_human Lysine-specific demethylase 5A Jumonji/ARID domain-containing protein 1A Retinoblastoma-binding protein 2 KDM5A RBBP2 RBP2 JARID1A Histone demethylase JARID1A [histone H3]-trimethyl-L-lysine(4) demethylase 5A protein protein human Homo sapiens Human MAGVGPGGYAAEFVPPPECPVFEPSWEEFTDPLSFIGRIRPLAEKTGICKIRPPKDWQPPFACEVKSFRFTPRVQRLNELEAMTRVRLDFLDQLAKFWELQGSTLKIPVVERKILDLYALSKIVASKGGFEMVTKEKKWSKVGSRLGYLPGKGTGSLLKSHYERILYPYELFQSGVSLMGVQMPNLDLKEKVEPEVLSTDTQTSPEPGTRMNILPKRTRRVKTQSESGDVSRNTELKKLQIFGAGPKVVGLAMGTKDKEDEVTRRRKVTNRSDAFNMQMRQRKGTLSVNFVDLYVCMFCGRGNNEDKLLLCDGCDDSYHTFCLIPPLPDVPKGDWRCPKCVAEECSKPREAFGFEQAVREYTLQSFGEMADNFKSDYFNMPVHMVPTELVEKEFWRLVSSIEEDVIVEYGADISSKDFGSGFPVKDGRRKILPEEEEYALSGWNLNNMPVLEQSVLAHINVDISGMKVPWLYVGMCFSSFCWHIEDHWSYSINYLHWGEPKTWYGVPSHAAEQLEEVMRELAPELFESQPDLLHQLVTIMNPNVLMEHGVPVYRTNQCAGEFVVTFPRAYHSGFNQGYNFAEAVNFCTADWLPIGRQCVNHYRRLRRHCVFSHEELIFKMAADPECLDVGLAAMVCKELTLMTEEETRLRESVVQMGVLMSEEEVFELVPDDERQCSACRTTCFLSALTCSCNPERLVCLYHPTDLCPCPMQKKCLRYRYPLEDLPSLLYGVKVRAQSYDTWVSRVTEALSANFNHKKDLIELRVMLEDAEDRKYPENDLFRKLRDAVKEAETCASVAQLLLSKKQKHRQSPDSGRTRTKLTVEELKAFVQQLFSLPCVISQARQVKNLLDDVEEFHERAQEAMMDETPDSSKLQMLIDMGSSLYVELPELPRLKQELQQARWLDEVRLTLSDPQQVTLDVMKKLIDSGVGLAPHHAVEKAMAELQELLTVSERWEEKAKVCLQARPRHSVASLESIVNEAKNIPAFLPNVLSLKEALQKAREWTAKVEAIQSGSNYAYLEQLESLSAKGRPIPVRLEALPQVESQVAAARAWRERTGRTFLKKNSSHTLLQVLSPRTDIGVYGSGKNRRKKVKELIEKEKEKDLDLEPLSDLEEGLEETRDTAMVVAVFKEREQKEIEAMHSLRAANLAKMTMVDRIEEVKFCICRKTASGFMLQCELCKDWFHNSCVPLPKSSSQKKGSSWQAKEVKFLCPLCMRSRRPRLETILSLLVSLQKLPVRLPEGEALQCLTERAMSWQDRARQALATDELSSALAKLSVLSQRMVEQAAREKTEKIISAELQKAAANPDLQGHLPSFQQSAFNRVVSSVSSSPRQTMDYDDEETDSDEDIRETYGYDMKDTASVKSSSSLEPNLFCDEEIPIKSEEVVTHMWTAPSFCAEHAYSSASKSCSQGSSTPRKQPRKSPLVPRSLEPPVLELSPGAKAQLEELMMVGDLLEVSLDETQHIWRILQATHPPSEDRFLHIMEDDSMEEKPLKVKGKDSSEKKRKRKLEKVEQLFGEGKQKSKELKKMDKPRKKKLKLGADKSKELNKLAKKLAKEEERKKKKEKAAAAKVELVKESTEKKREKKVLDIPSKYDWSGAEESDDENAVCAAQNCQRPCKDKVDWVQCDGGCDEWFHQVCVGVSPEMAENEDYICINCAKKQGPVSPGPAPPPSFIMSYKLPMEDLKETS h3peptide-kdm5a-1 3190501 N/A 3190506 unspecified role unspecified role unspecified role unspecified role Region 1-9 Region 1-9 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3190507 unspecified role unspecified role unspecified role unspecified role Region 1601-1660 Region 1601-1660 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false h3peptide-kdm5a-2 3190502 N/A 3190506 unspecified role unspecified role unspecified role unspecified role Region 1-9 Region 1-9 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3190507 unspecified role unspecified role unspecified role unspecified role Region 1601-1660 Region 1601-1660 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false h3peptide-kdm5a-3 3190503 N/A 3190506 unspecified role unspecified role bait bait K4me2 K4me2 n6me2lys N6,N6-dimethyl-L-lysine (2S)-2-amino-6-(dimethylamino)hexanoic acid MOD_RES N6,N6-dimethyllysine N(zeta)-dimethyllysine N6,N6-dimethyl-L-lysine N6,N6-dimethylated L-lysine epsilon-dimethyllysine lysine derivative Lys(y) Dimethyl N6Me2Lys di-Methylation dimethylk certain certain sequence position certain certain certain sequence position certain false K4me3 K4me3 n6me3+lys N6,N6,N6-trimethyl-L-lysine (5S)-5-amino-5-carboxy-N,N,N-trimethylpentan-1-aminium 2-amino-6-(trimethylammonio)hexanoic acid 5-azanyl-5-carboxy-N,N,N-trimethylpentanazanium MOD_RES N6,N6,N6-trimethyllysine N(zeta)-trimethyllysine N6,N6,N6-trimethyl-L-lysine N6,N6,N6-trimethylated L-lysine N6,N6,N6-trimethyllysin-N6-ium N6,N6,N6-trimethyllysine cation epsilon-trimethyllysine N-trimethylation (of lysine) N6Me3+Lys Trimethyl tri-Methylation certain certain sequence position certain certain certain sequence position certain false Biotin-tag Biotin-tag biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro N/A 3190507 unspecified role unspecified role prey prey Region 1601-1660 Region 1601-1660 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false P29375:p.Trp1625Ala W1625A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P29375:p.[Glu1634Ala;Trp1635Ala] E1634A/W1635A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false h3peptide-kdm5a-4 3190504 N/A 3190506 unspecified role unspecified role unspecified role unspecified role Region 1-15 Region 1-15 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3190507 unspecified role unspecified role unspecified role unspecified role Region 1601-1660 Region 1601-1660 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false h3peptide-kdm5a-5 3190505 N/A 3190506 unspecified role unspecified role unspecified role unspecified role Region 1-9 Region 1-9 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3190507 unspecified role unspecified role unspecified role unspecified role Region 1601-1660 Region 1601-1660 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false