DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ berry-2009-1 in vitro In vitro protease assay protease assay weight silver stain molecular weight estimation by silver staining weight silver stain Berry R., Jowitt TA., Ferrand J., Roessle M., Grossmann JG., Canty-Laird EG., Kammerer RA., Kadler KE., Baldock C. DIP original experiment: DIP-134219X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Role of dimerization and substrate exclusion in the regulation of bone morphogenetic protein-1 and mammalian tolloid. Berry R., Jowitt TA., Ferrand J., Roessle M., Grossmann JG., Canty-Laird EG., Kammerer RA., Kadler KE., Baldock C. Only protein-protein interactions imex curation berry-2009-2 human Homo sapiens Human molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Berry R., Jowitt TA., Ferrand J., Roessle M., Grossmann JG., Canty-Laird EG., Kammerer RA., Kadler KE., Baldock C. DIP original experiment: DIP-134222X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Role of dimerization and substrate exclusion in the regulation of bone morphogenetic protein-1 and mammalian tolloid. Berry R., Jowitt TA., Ferrand J., Roessle M., Grossmann JG., Canty-Laird EG., Kammerer RA., Kadler KE., Baldock C. Only protein-protein interactions imex curation berry-2009-3 human Homo sapiens Human tem transmission electron microscopy tem predetermined predetermined participant predetermined Berry R., Jowitt TA., Ferrand J., Roessle M., Grossmann JG., Canty-Laird EG., Kammerer RA., Kadler KE., Baldock C. DIP original experiment: DIP-134223X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Role of dimerization and substrate exclusion in the regulation of bone morphogenetic protein-1 and mammalian tolloid. Berry R., Jowitt TA., Ferrand J., Roessle M., Grossmann JG., Canty-Laird EG., Kammerer RA., Kadler KE., Baldock C. Only protein-protein interactions imex curation berry-2009-4 human Homo sapiens Human light scattering light scattering predetermined predetermined participant predetermined Berry R., Jowitt TA., Ferrand J., Roessle M., Grossmann JG., Canty-Laird EG., Kammerer RA., Kadler KE., Baldock C. DIP original experiment: DIP-134220X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Role of dimerization and substrate exclusion in the regulation of bone morphogenetic protein-1 and mammalian tolloid. Berry R., Jowitt TA., Ferrand J., Roessle M., Grossmann JG., Canty-Laird EG., Kammerer RA., Kadler KE., Baldock C. Only protein-protein interactions imex curation berry-2009-5 in vitro In vitro protease assay protease assay predetermined predetermined participant predetermined Berry R., Jowitt TA., Ferrand J., Roessle M., Grossmann JG., Canty-Laird EG., Kammerer RA., Kadler KE., Baldock C. DIP original experiment: DIP-134224X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Role of dimerization and substrate exclusion in the regulation of bone morphogenetic protein-1 and mammalian tolloid. Berry R., Jowitt TA., Ferrand J., Roessle M., Grossmann JG., Canty-Laird EG., Kammerer RA., Kadler KE., Baldock C. Only protein-protein interactions imex curation berry-2009-6 human Homo sapiens Human solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Berry R., Jowitt TA., Ferrand J., Roessle M., Grossmann JG., Canty-Laird EG., Kammerer RA., Kadler KE., Baldock C. DIP original experiment: DIP-134221X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Role of dimerization and substrate exclusion in the regulation of bone morphogenetic protein-1 and mammalian tolloid. Berry R., Jowitt TA., Ferrand J., Roessle M., Grossmann JG., Canty-Laird EG., Kammerer RA., Kadler KE., Baldock C. Only protein-protein interactions imex curation chrd_human Chordin CHRD UNQ217/PRO243 protein protein human Homo sapiens Human MPSLPAPPAPLLLLGLLLLGSRPARGAGPEPPVLPIRSEKEPLPVRGAAGCTFGGKVYALDETWHPDLGEPFGVMRCVLCACEAPQWGRRTRGPGRVSCKNIKPECPTPACGQPRQLPGHCCQTCPQERSSSERQPSGLSFEYPRDPEHRSYSDRGEPGAEERARGDGHTDFVALLTGPRSQAVARARVSLLRSSLRFSISYRRLDRPTRIRFSDSNGSVLFEHPAAPTQDGLVCGVWRAVPRLSLRLLRAEQLHVALVTLTHPSGEVWGPLIRHRALAAETFSAILTLEGPPQQGVGGITLLTLSDTEDSLHFLLLFRGLLEPRSGGLTQVPLRLQILHQGQLLRELQANVSAQEPGFAEVLPNLTVQEMDWLVLGELQMALEWAGRPGLRISGHIAARKSCDVLQSVLCGADALIPVQTGAAGSASLTLLGNGSLIYQVQVVGTSSEVVAMTLETKPQRRDQRTVLCHMAGLQPGGHTAVGICPGLGARGAHMLLQNELFLNVGTKDFPDGELRGHVAALPYCGHSARHDTLPVPLAGALVLPPVKSQAAGHAWLSLDTHCHLHYEVLLAGLGGSEQGTVTAHLLGPPGTPGPRRLLKGFYGSEAQGVVKDLEPELLRHLAKGMASLMITTKGSPRGELRGQVHIANQCEVGGLRLEAAGAEGVRALGAPDTASAAPPVVPGLPALAPAKPGGPGRPRDPNTCFFEGQQRPHGARWAPNYDPLCSLCTCQRRTVICDPVVCPPPSCPHPVQAPDQCCPVCPEKQDVRDLPGLPRSRDPGEGCYFDGDRSWRAAGTRWHPVVPPFGLIKCAVCTCKGGTGEVHCEKVQCPRLACAQPVRVNPTDCCKQCPVGSGAHPQLGDPMQADGPRGCRFAGQWFPESQSWHPSVPPFGEMSCITCRCGAGVPHCERDDCSLPLSCGSGKESRCCSRCTAHRRPAPETRTDPELEKEAEGS bmp1_human Bone morphogenetic protein 1 Procollagen C-proteinase Mammalian tolloid protein BMP1 PCOLC protein protein human Homo sapiens Human MPGVARLPLLLGLLLLPRPGRPLDLADYTYDLAEEDDSEPLNYKDPCKAAAFLGDIALDEEDLRAFQVQQAVDLRRHTARKSSIKAAVPGNTSTPSCQSTNGQPQRGACGRWRGRSRSRRAATSRPERVWPDGVIPFVIGGNFTGSQRAVFRQAMRHWEKHTCVTFLERTDEDSYIVFTYRPCGCCSYVGRRGGGPQAISIGKNCDKFGIVVHELGHVVGFWHEHTRPDRDRHVSIVRENIQPGQEYNFLKMEPQEVESLGETYDFDSIMHYARNTFSRGIFLDTIVPKYEVNGVKPPIGQRTRLSKGDIAQARKLYKCPACGETLQDSTGNFSSPEYPNGYSAHMHCVWRISVTPGEKIILNFTSLDLYRSRLCWYDYVEVRDGFWRKAPLRGRFCGSKLPEPIVSTDSRLWVEFRSSSNWVGKGFFAVYEAICGGDVKKDYGHIQSPNYPDDYRPSKVCIWRIQVSEGFHVGLTFQSFEIERHDSCAYDYLEVRDGHSESSTLIGRYCGYEKPDDIKSTSSRLWLKFVSDGSINKAGFAVNFFKEVDECSRPNRGGCEQRCLNTLGSYKCSCDPGYELAPDKRRCEAACGGFLTKLNGSITSPGWPKEYPPNKNCIWQLVAPTQYRISLQFDFFETEGNDVCKYDFVEVRSGLTADSKLHGKFCGSEKPEVITSQYNNMRVEFKSDNTVSKKGFKAHFFSDKDECSKDNGGCQQDCVNTFGSYECQCRSGFVLHDNKHDCKEAGCDHKVTSTSGTITSPNWPDKYPSKKECTWAISSTPGHRVKLTFMEMDIESQPECAYDHLEVFDGRDAKAPVLGRFCGSKKPEPVLATGSRMFLRFYSDNSVQRKGFQASHATECGGQVRADVKTKDLYSHAQFGDNNYPGGVDCEWVIVAEEGYGVELVFQTFEVEEETDCGYDYMELFDGYDSTAPRLGRYCGSGPPEEVYSAGDSVLVKFHSDDTITKKGFHLRYTSTKFQDTLHSRK p13497-2 Bone morphogenetic protein 1 BMP1 PCOLC Mammalian tolloid protein Procollagen C-proteinase protein protein human Homo sapiens Human MPGVARLPLLLGLLLLPRPGRPLDLADYTYDLAEEDDSEPLNYKDPCKAAAFLGDIALDEEDLRAFQVQQAVDLRRHTARKSSIKAAVPGNTSTPSCQSTNGQPQRGACGRWRGRSRSRRAATSRPERVWPDGVIPFVIGGNFTGSQRAVFRQAMRHWEKHTCVTFLERTDEDSYIVFTYRPCGCCSYVGRRGGGPQAISIGKNCDKFGIVVHELGHVVGFWHEHTRPDRDRHVSIVRENIQPGQEYNFLKMEPQEVESLGETYDFDSIMHYARNTFSRGIFLDTIVPKYEVNGVKPPIGQRTRLSKGDIAQARKLYKCPACGETLQDSTGNFSSPEYPNGYSAHMHCVWRISVTPGEKIILNFTSLDLYRSRLCWYDYVEVRDGFWRKAPLRGRFCGSKLPEPIVSTDSRLWVEFRSSSNWVGKGFFAVYEAICGGDVKKDYGHIQSPNYPDDYRPSKVCIWRIQVSEGFHVGLTFQSFEIERHDSCAYDYLEVRDGHSESSTLIGRYCGYEKPDDIKSTSSRLWLKFVSDGSINKAGFAVNFFKEVDECSRPNRGGCEQRCLNTLGSYKCSCDPGYELAPDKRRCEAACGGFLTKLNGSITSPGWPKEYPPNKNCIWQLVAPTQYRISLQFDFFETEGNDVCKYDFVEVRSGLTADSKLHGKFCGSEKPEVITSQYNNMRVEFKSDNTVSKKGFKAHFFSEKRPALQPPRGRPHQLKFRVQKRNRTPQ bmp1-chrd-1 3190471 N/A 3190477 enzyme target enzyme target substrate substrate unspecified role unspecified role human Homo sapiens Human N/A 3190478 enzyme enzyme unspecified role unspecified role Region 1-743 Region 1-743 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human cleavage cleavage reaction cleavage false false false bmp1-1 3190472 N/A 3190478 unspecified role unspecified role unspecified role unspecified role human Homo sapiens Human direct interaction direct interaction false false false bmp1-2 3190473 N/A 3190478 unspecified role unspecified role unspecified role unspecified role human Homo sapiens Human direct interaction direct interaction false false false bmp1-3 3190474 N/A 3190478 unspecified role unspecified role unspecified role unspecified role human Homo sapiens Human direct interaction direct interaction false false false bmp1-chrd-2 3190475 N/A 3190479 enzyme enzyme unspecified role unspecified role human Homo sapiens Human N/A 3190477 enzyme target enzyme target substrate substrate unspecified role unspecified role human Homo sapiens Human cleavage cleavage reaction cleavage false false false bmp1-4 3190476 N/A 3190478 unspecified role unspecified role unspecified role unspecified role human Homo sapiens Human direct interaction direct interaction false false false