DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ maglica-2009-1 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Maglica Z., Kolygo K., Weber-Ban E. DIP original experiment: DIP-132075X Structure (London, England : 1993) 2009 IMEx Optimal efficiency of ClpAP and ClpXP chaperone-proteases is achieved by architectural symmetry. Maglica Z., Kolygo K., Weber-Ban E. Only protein-protein interactions imex curation maglica-2009-2 in vitro In vitro 3d-em 3D electron microscopy 3D-EM electron tomog predetermined predetermined participant predetermined Maglica Z., Kolygo K., Weber-Ban E. DIP original experiment: DIP-132080X Structure (London, England : 1993) 2009 IMEx Optimal efficiency of ClpAP and ClpXP chaperone-proteases is achieved by architectural symmetry. Maglica Z., Kolygo K., Weber-Ban E. Only protein-protein interactions imex curation maglica-2009-3 in vitro In vitro enzymatic study enzymatic study predetermined predetermined participant predetermined Maglica Z., Kolygo K., Weber-Ban E. DIP original experiment: DIP-132078X Structure (London, England : 1993) 2009 IMEx Optimal efficiency of ClpAP and ClpXP chaperone-proteases is achieved by architectural symmetry. Maglica Z., Kolygo K., Weber-Ban E. Only protein-protein interactions imex curation maglica-2009-4 in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Maglica Z., Kolygo K., Weber-Ban E. DIP original experiment: DIP-132081X Structure (London, England : 1993) 2009 IMEx Optimal efficiency of ClpAP and ClpXP chaperone-proteases is achieved by architectural symmetry. Maglica Z., Kolygo K., Weber-Ban E. Only protein-protein interactions imex curation maglica-2009-5 in vitro In vitro ion exchange chrom ion exchange chromatography IEC IEC ion exchange chrom predetermined predetermined participant predetermined Maglica Z., Kolygo K., Weber-Ban E. DIP original experiment: DIP-132076X Structure (London, England : 1993) 2009 IMEx Optimal efficiency of ClpAP and ClpXP chaperone-proteases is achieved by architectural symmetry. Maglica Z., Kolygo K., Weber-Ban E. Only protein-protein interactions imex curation maglica-2009-6 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Maglica Z., Kolygo K., Weber-Ban E. DIP original experiment: DIP-132079X Structure (London, England : 1993) 2009 IMEx Optimal efficiency of ClpAP and ClpXP chaperone-proteases is achieved by architectural symmetry. Maglica Z., Kolygo K., Weber-Ban E. Only protein-protein interactions imex curation maglica-2009-7 in vitro In vitro enzymatic study enzymatic study predetermined predetermined participant predetermined Maglica Z., Kolygo K., Weber-Ban E. DIP original experiment: DIP-132077X Structure (London, England : 1993) 2009 IMEx Optimal efficiency of ClpAP and ClpXP chaperone-proteases is achieved by architectural symmetry. Maglica Z., Kolygo K., Weber-Ban E. Only protein-protein interactions imex curation maglica-2009-8 in vitro In vitro light scattering light scattering predetermined predetermined participant predetermined Maglica Z., Kolygo K., Weber-Ban E. DIP original experiment: DIP-132082X Structure (London, England : 1993) 2009 IMEx Optimal efficiency of ClpAP and ClpXP chaperone-proteases is achieved by architectural symmetry. Maglica Z., Kolygo K., Weber-Ban E. Only protein-protein interactions imex curation clpp_ecoli ATP-dependent Clp protease proteolytic subunit clpP lopP Endopeptidase Clp Caseinolytic protease Protease Ti Heat shock protein F21.5 b0437 JW0427 protein protein ecoli Escherichia coli (strain K12) MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIFLTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVSTICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVKGRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN clpa_ecoli ATP-dependent Clp protease ATP-binding subunit ClpA clpA lopD b0882 JW0866 protein protein ecoli Escherichia coli (strain K12) MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREALEACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNEVTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSEEQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGVGKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQDTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKDRALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDRHLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLKNLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQLSKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEIEKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDNSTDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEVSQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKNELTYGFQSAQKHKAEAAH clpx_ecoli ATP-dependent Clp protease ATP-binding subunit ClpX clpX lopC b0438 JW0428 ATP-dependent unfoldase ClpX protein protein ecoli Escherichia coli (strain K12) MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLCNDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNGDTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVENIIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGTVAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKAKSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALTKQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMEDVEKVVIDESVIDGQSKPLLIYGKPEAQQASGE clpp-clpp-1 3187008 N/A 3187016 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3187016 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecolx Escherichia coli direct interaction direct interaction false false false clpa-clpp-2 3187009 N/A 3187017 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3187016 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false clpp-clpx-1 3187010 N/A 3187018 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3187016 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false clpa-clpp-3 3187011 N/A 3187017 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3187016 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false clpp-clpp-2 3187012 N/A 3187016 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3187016 unspecified role unspecified role unspecified role unspecified role His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecolx Escherichia coli direct interaction direct interaction false false false clpa-clpp-4 3187013 N/A 3187017 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3187016 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false clpa-clpp-5 3187014 N/A 3187017 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3187016 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false clpa-clpp-6 3187015 N/A 3187017 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3187016 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false