DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ yuan-2009-1 ecolx Escherichia coli lambda two hybrid lambda repressor two hybrid BacterioMatch BacterioMatch lambda two hybrid predetermined predetermined participant predetermined Yuan AH., Nickels BE., Hochschild A. DIP original experiment: DIP-260242X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx The bacteriophage T4 AsiA protein contacts the beta-flap domain of RNA polymerase. Yuan AH., Nickels BE., Hochschild A. Only protein-protein interactions imex curation yuan-2009-2 ecolx Escherichia coli lambda two hybrid lambda repressor two hybrid BacterioMatch BacterioMatch lambda two hybrid predetermined predetermined participant predetermined Yuan AH., Nickels BE., Hochschild A. DIP original experiment: DIP-260240X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx The bacteriophage T4 AsiA protein contacts the beta-flap domain of RNA polymerase. Yuan AH., Nickels BE., Hochschild A. Only protein-protein interactions imex curation yuan-2009-3 in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel predetermined predetermined participant predetermined Yuan AH., Nickels BE., Hochschild A. DIP original experiment: DIP-260245X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx The bacteriophage T4 AsiA protein contacts the beta-flap domain of RNA polymerase. Yuan AH., Nickels BE., Hochschild A. Only protein-protein interactions imex curation yuan-2009-4 ecolx Escherichia coli lambda two hybrid lambda repressor two hybrid BacterioMatch BacterioMatch lambda two hybrid predetermined predetermined participant predetermined Yuan AH., Nickels BE., Hochschild A. DIP original experiment: DIP-260243X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx The bacteriophage T4 AsiA protein contacts the beta-flap domain of RNA polymerase. Yuan AH., Nickels BE., Hochschild A. Only protein-protein interactions imex curation yuan-2009-5 ecolx Escherichia coli lambda two hybrid lambda repressor two hybrid BacterioMatch BacterioMatch lambda two hybrid predetermined predetermined participant predetermined Yuan AH., Nickels BE., Hochschild A. DIP original experiment: DIP-260241X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx The bacteriophage T4 AsiA protein contacts the beta-flap domain of RNA polymerase. Yuan AH., Nickels BE., Hochschild A. Only protein-protein interactions imex curation yuan-2009-6 ecolx Escherichia coli lambda two hybrid lambda repressor two hybrid BacterioMatch BacterioMatch lambda two hybrid predetermined predetermined participant predetermined Yuan AH., Nickels BE., Hochschild A. DIP original experiment: DIP-260244X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx The bacteriophage T4 AsiA protein contacts the beta-flap domain of RNA polymerase. Yuan AH., Nickels BE., Hochschild A. Only protein-protein interactions imex curation rpob_ecoli DNA-directed RNA polymerase subunit beta rpoB groN nitB rif ron stl stv tabD b3987 Transcriptase subunit beta RNA polymerase subunit beta JW3950 protein protein ecoli Escherichia coli (strain K12) MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPEGQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRLVIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSDKGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTEQILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQLEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIETLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSEDRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGNRRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGSSQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLDEEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMGANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDEMYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQNMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPNVGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVKDSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLFSRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLEAKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYDENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQRAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDLPTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMPESFNVLLKEIRSLGINIELEDE asia_bpt4 10 kDa anti-sigma factor 10 kDa RNA polymerase-associated protein asiA Audrey Stevens' inhibitor protein protein bpt4 Enterobacteria phage T4 Bacteriophage T4 MNKNIDTVREIITVASILIKFSREDIVENRANFIAFLNEIGVTHEGRKLNQNSFRKIVSELTQEDKKTLIDEFNEGFEGVYRYLEMYTNK rpod_ecoli RNA polymerase sigma factor RpoD rpoD alt b3067 Sigma-70 JW3039 protein protein ecoli Escherichia coli (strain K12) MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDIIQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYMREMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSDLITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAREKFAELRAQYVVTRDTIKAKGRSHATAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVMMDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVSEEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISMETPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGIDMNTDYTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD asia-rpob-1 3186973 N/A 3186979 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3186980 unspecified role unspecified role bait bait P32267:p.Asn74Asp N74D mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false asia-rpob-2 3186974 N/A 3186979 unspecified role unspecified role prey prey Region 900-909 Region 900-909 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false P0A8V2:p.Gly907Lys G907K mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false P0A8V2:p.[Ile905Ala;Phe906Ala] I905A/F906A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3186980 unspecified role unspecified role bait bait ecolx Escherichia coli physical association physical association false false false asia-rpod-1 3186975 N/A 3186981 unspecified role unspecified role unspecified role unspecified role P00579:p.Phe563Tyr F563Y mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3186980 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false asia-rpod-2 3186976 N/A 3186981 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3186980 unspecified role unspecified role bait bait ecolx Escherichia coli physical association physical association false false false rpob-rpod-3 3186977 N/A 3186981 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3186979 unspecified role unspecified role bait bait Region 900-909 Region 900-909 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false rpob-rpod-4 3186978 N/A 3186981 unspecified role unspecified role prey prey P00579:p.Phe563Tyr F563Y mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false P00579:p.Leu607Pro L607P mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3186979 unspecified role unspecified role bait bait ecolx Escherichia coli physical association physical association false false false