DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ adinolfi-2009-1 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Adinolfi S., Iannuzzi C., Prischi F., Pastore C., Iametti S., Martin SR., Bonomi F., Pastore A. DIP original experiment: DIP-133001X Nature structural & molecular biology 2009 IMEx Bacterial frataxin CyaY is the gatekeeper of iron-sulfur cluster formation catalyzed by IscS. Adinolfi S., Iannuzzi C., Prischi F., Pastore C., Iametti S., Martin SR., Bonomi F., Pastore A. Only protein-protein interactions imex curation adinolfi-2009-2 in vitro In vitro enzymatic study enzymatic study predetermined predetermined participant predetermined Adinolfi S., Iannuzzi C., Prischi F., Pastore C., Iametti S., Martin SR., Bonomi F., Pastore A. DIP original experiment: DIP-132998X Nature structural & molecular biology 2009 IMEx Bacterial frataxin CyaY is the gatekeeper of iron-sulfur cluster formation catalyzed by IscS. Adinolfi S., Iannuzzi C., Prischi F., Pastore C., Iametti S., Martin SR., Bonomi F., Pastore A. Only protein-protein interactions imex curation adinolfi-2009-3 in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Adinolfi S., Iannuzzi C., Prischi F., Pastore C., Iametti S., Martin SR., Bonomi F., Pastore A. DIP original experiment: DIP-133002X Nature structural & molecular biology 2009 IMEx Bacterial frataxin CyaY is the gatekeeper of iron-sulfur cluster formation catalyzed by IscS. Adinolfi S., Iannuzzi C., Prischi F., Pastore C., Iametti S., Martin SR., Bonomi F., Pastore A. Only protein-protein interactions imex curation adinolfi-2009-4 in vitro In vitro enzymatic study enzymatic study predetermined predetermined participant predetermined Adinolfi S., Iannuzzi C., Prischi F., Pastore C., Iametti S., Martin SR., Bonomi F., Pastore A. DIP original experiment: DIP-133004X Nature structural & molecular biology 2009 IMEx Bacterial frataxin CyaY is the gatekeeper of iron-sulfur cluster formation catalyzed by IscS. Adinolfi S., Iannuzzi C., Prischi F., Pastore C., Iametti S., Martin SR., Bonomi F., Pastore A. Only protein-protein interactions imex curation adinolfi-2009-5 in vitro In vitro enzymatic study enzymatic study predetermined predetermined participant predetermined Adinolfi S., Iannuzzi C., Prischi F., Pastore C., Iametti S., Martin SR., Bonomi F., Pastore A. DIP original experiment: DIP-132999X Nature structural & molecular biology 2009 IMEx Bacterial frataxin CyaY is the gatekeeper of iron-sulfur cluster formation catalyzed by IscS. Adinolfi S., Iannuzzi C., Prischi F., Pastore C., Iametti S., Martin SR., Bonomi F., Pastore A. Only protein-protein interactions imex curation adinolfi-2009-6 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Adinolfi S., Iannuzzi C., Prischi F., Pastore C., Iametti S., Martin SR., Bonomi F., Pastore A. DIP original experiment: DIP-133000X Nature structural & molecular biology 2009 IMEx Bacterial frataxin CyaY is the gatekeeper of iron-sulfur cluster formation catalyzed by IscS. Adinolfi S., Iannuzzi C., Prischi F., Pastore C., Iametti S., Martin SR., Bonomi F., Pastore A. Only protein-protein interactions imex curation adinolfi-2009-7 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Adinolfi S., Iannuzzi C., Prischi F., Pastore C., Iametti S., Martin SR., Bonomi F., Pastore A. DIP original experiment: DIP-133003X Nature structural & molecular biology 2009 IMEx Bacterial frataxin CyaY is the gatekeeper of iron-sulfur cluster formation catalyzed by IscS. Adinolfi S., Iannuzzi C., Prischi F., Pastore C., Iametti S., Martin SR., Bonomi F., Pastore A. Only protein-protein interactions imex curation iscs_ecoli Cysteine desulfurase IscS iscS b2530 JW2514 yfhO yzzO ThiI transpersulfidase NifS protein homolog TusA transpersulfidase nuvC protein protein ecoli Escherichia coli (strain K12) MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFGWQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKTEHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVVQDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYVRRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNRLWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLEPSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQGVDLNSIEWAHH 1E4F2E6F9CD266B0 cyay_ecoli Iron-sulfur cluster assembly protein CyaY Bacterial frataxin ortholog JW3779 b3807 cyaY protein protein ecoli Escherichia coli (strain K12) MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFENGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGETVSFR fer_ecoli 2Fe-2S ferredoxin fdx b2525 JW2509 protein protein ecoli Escherichia coli (strain K12) MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEKSCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPRYTINHAREH iscu_ecoli Iron-sulfur cluster assembly scaffold protein IscU iscU nifU yfhN JW2513 b2529 Sulfur acceptor protein IscU protein protein ecoli Escherichia coli (strain K12) MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQIKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPVKIHCSILAEDAIKAAIADYKSKREAK cyay-iscs-1 3186054 N/A 3186061 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3186062 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false fdx-iscs-1 3186055 N/A 3186063 enzyme target enzyme target substrate substrate unspecified role unspecified role ecolx Escherichia coli N/A 3186061 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli enzymatic reaction enzymatic reaction Biochemical reaction Biochemical reaction false false false iscs-iscu-4 3186056 N/A 3186064 unspecified role unspecified role unspecified role unspecified role Alexa532-label Alexa532-label alexa 532 label alexa 532 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli N/A 3186061 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false fdx-iscu-1 3186057 N/A 3186064 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli N/A 3186063 enzyme target enzyme target substrate substrate unspecified role unspecified role ecolx Escherichia coli enzymatic reaction enzymatic reaction Biochemical reaction Biochemical reaction false false false iscs-iscu-5 3186058 N/A 3186064 enzyme target enzyme target substrate substrate unspecified role unspecified role ecolx Escherichia coli N/A 3186061 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli enzymatic reaction enzymatic reaction Biochemical reaction Biochemical reaction false false false cyay-1 3186059 N/A 3186062 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false cyay-iscs-2 3186060 N/A 3186061 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3186062 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli physical association physical association false false false