DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ ren-2009-4 human Homo sapiens Human molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Ren X., Kloer DP., Kim YC., Ghirlando R., Saidi LF., Hummer G., Hurley JH. DIP original experiment: DIP-132112X Structure (London, England : 1993) 2009 IMEx Hybrid structural model of the complete human ESCRT-0 complex. Ren X., Kloer DP., Kim YC., Ghirlando R., Saidi LF., Hummer G., Hurley JH. Only protein-protein interactions imex curation ren-2009-5 trini Trichoplusia ni (Cabbage looper) Cabbage looper dls dynamic light scattering dls predetermined predetermined participant predetermined Ren X., Kloer DP., Kim YC., Ghirlando R., Saidi LF., Hummer G., Hurley JH. DIP original experiment: DIP-132115X Structure (London, England : 1993) 2009 IMEx Hybrid structural model of the complete human ESCRT-0 complex. Ren X., Kloer DP., Kim YC., Ghirlando R., Saidi LF., Hummer G., Hurley JH. Only protein-protein interactions imex curation ren-2009-6 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Ren X., Kloer DP., Kim YC., Ghirlando R., Saidi LF., Hummer G., Hurley JH. DIP original experiment: DIP-132119X Structure (London, England : 1993) 2009 IMEx Hybrid structural model of the complete human ESCRT-0 complex. Ren X., Kloer DP., Kim YC., Ghirlando R., Saidi LF., Hummer G., Hurley JH. Only protein-protein interactions imex curation ren-2009-7 ecolx Escherichia coli dls dynamic light scattering dls predetermined predetermined participant predetermined Ren X., Kloer DP., Kim YC., Ghirlando R., Saidi LF., Hummer G., Hurley JH. DIP original experiment: DIP-132117X Structure (London, England : 1993) 2009 IMEx Hybrid structural model of the complete human ESCRT-0 complex. Ren X., Kloer DP., Kim YC., Ghirlando R., Saidi LF., Hummer G., Hurley JH. Only protein-protein interactions imex curation ren-2009-8 ecolx Escherichia coli light scattering light scattering predetermined predetermined participant predetermined Ren X., Kloer DP., Kim YC., Ghirlando R., Saidi LF., Hummer G., Hurley JH. DIP original experiment: DIP-132122X Structure (London, England : 1993) 2009 IMEx Hybrid structural model of the complete human ESCRT-0 complex. Ren X., Kloer DP., Kim YC., Ghirlando R., Saidi LF., Hummer G., Hurley JH. Only protein-protein interactions imex curation ren-2009-9 trini Trichoplusia ni (Cabbage looper) Cabbage looper light scattering light scattering predetermined predetermined participant predetermined Ren X., Kloer DP., Kim YC., Ghirlando R., Saidi LF., Hummer G., Hurley JH. DIP original experiment: DIP-132121X Structure (London, England : 1993) 2009 IMEx Hybrid structural model of the complete human ESCRT-0 complex. Ren X., Kloer DP., Kim YC., Ghirlando R., Saidi LF., Hummer G., Hurley JH. Only protein-protein interactions imex curation ren-2009-10 trini Trichoplusia ni (Cabbage looper) Cabbage looper molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Ren X., Kloer DP., Kim YC., Ghirlando R., Saidi LF., Hummer G., Hurley JH. DIP original experiment: DIP-132113X Structure (London, England : 1993) 2009 IMEx Hybrid structural model of the complete human ESCRT-0 complex. Ren X., Kloer DP., Kim YC., Ghirlando R., Saidi LF., Hummer G., Hurley JH. Only protein-protein interactions imex curation ren-2009-11 ecolx Escherichia coli solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Ren X., Kloer DP., Kim YC., Ghirlando R., Saidi LF., Hummer G., Hurley JH. DIP original experiment: DIP-132116X Structure (London, England : 1993) 2009 IMEx Hybrid structural model of the complete human ESCRT-0 complex. Ren X., Kloer DP., Kim YC., Ghirlando R., Saidi LF., Hummer G., Hurley JH. Only protein-protein interactions imex curation ren-2009-12 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Ren X., Kloer DP., Kim YC., Ghirlando R., Saidi LF., Hummer G., Hurley JH. DIP original experiment: DIP-132120X Structure (London, England : 1993) 2009 IMEx Hybrid structural model of the complete human ESCRT-0 complex. Ren X., Kloer DP., Kim YC., Ghirlando R., Saidi LF., Hummer G., Hurley JH. Only protein-protein interactions imex curation ren-2009-13 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Ren X., Kloer DP., Kim YC., Ghirlando R., Saidi LF., Hummer G., Hurley JH. DIP original experiment: DIP-132118X Structure (London, England : 1993) 2009 IMEx Hybrid structural model of the complete human ESCRT-0 complex. Ren X., Kloer DP., Kim YC., Ghirlando R., Saidi LF., Hummer G., Hurley JH. Only protein-protein interactions imex curation ren-2009-14 trini Trichoplusia ni (Cabbage looper) Cabbage looper solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Ren X., Kloer DP., Kim YC., Ghirlando R., Saidi LF., Hummer G., Hurley JH. DIP original experiment: DIP-132114X Structure (London, England : 1993) 2009 IMEx Hybrid structural model of the complete human ESCRT-0 complex. Ren X., Kloer DP., Kim YC., Ghirlando R., Saidi LF., Hummer G., Hurley JH. Only protein-protein interactions imex curation stam1_human Signal transducing adapter molecule 1 STAM STAM1 protein protein human Homo sapiens Human MPLFATNPFDQDVEKATSEMNTAEDWGLILDICDKVGQSRTGPKDCLRSIMRRVNHKDPHVAMQALTLLGACVSNCGKIFHLEVCSRDFASEVSNVLNKGHPKVCEKLKALMVEWTDEFKNDPQLSLISAMIKNLKEQGVTFPAIGSQAAEQAKASPALVAKDPGTVANKKEEEDLAKAIELSLKEQRQQSTTLSTLYPSTSSLLTNHQHEGRKVRAIYDFEAAEDNELTFKAGEIITVLDDSDPNWWKGETHQGIGLFPSNFVTADLTAEPEMIKTEKKTVQFSDDVQVETIEPEPEPAFIDEDKMDQLLQMLQSTDPSDDQPDLPELLHLEAMCHQMGPLIDEKLEDIDRKHSELSELNVKVMEALSLYTKLMNEDPMYSMYAKLQNQPYYMQSSGVSGSQVYAGPPPSGAYLVAGNAQMSHLQSYSLPPEQLSSLSQAVVPPSANPALPSQQTQAAYPNTMVSSVQGNTYPSQAPVYSPPPAATAAAATADVTLYQNAGPNMPQVPNYNLTSSTLPQPGGSQQPPQPQQPYSQKALL hgs_human Hepatocyte growth factor-regulated tyrosine kinase substrate Protein pp110 Hrs HGS HRS protein protein human Homo sapiens Human MGRGSGTFERLLDKATSQLLLETDWESILQICDLIRQGDTQAKYAVNSIKKKVNDKNPHVALYALEVMESVVKNCGQTVHDEVANKQTMEELKDLLKRQVEVNVRNKILYLIQAWAHAFRNEPKYKVVQDTYQIMKVEGHVFPEFKESDAMFAAERAPDWVDAEECHRCRVQFGVMTRKHHCRACGQIFCGKCSSKYSTIPKFGIEKEVRVCEPCYEQLNRKAEGKATSTTELPPEYLTSPLSQQSQLPPKRDETALQEEEELQLALALSQSEAEEKERLRQKSTYTSYPKAEPMPSASSAPPASSLYSSPVNSSAPLAEDIDPELARYLNRNYWEKKQEEARKSPTPSAPVPLTEPAAQPGEGHAAPTNVVENPLPETDSQPIPPSGGPFSEPQFHNGESEESHEQFLKALQNAVTTFVNRMKSNHMRGRSITNDSAVLSLFQSINGMHPQLLELLNQLDERRLYYEGLQDKLAQIRDARGALSALREEHREKLRRAAEEAERQRQIQLAQKLEIMRQKKQEYLEVQRQLAIQRLQEQEKERQMRLEQQKQTVQMRAQMPAFPLPYAQLQAMPAAGGVLYQPSGPASFPSTFSPAGSVEGSPMHGVYMSQPAPAAGPYPSMPSTAADPSMVSAYMYPAGATGAQAAPQAQAGPTASPAYSSYQPTPTAGYQNVASQAPQSLPAISQPPQSSTMGYMGSQSVSMGYQPYNMQNLMTTLPSQDASLPPQQPYIAGQQPMYQQMAPSGGPPQQQPPVAQQPQAQGPPAQGSEAQLISFD hgs_mouse Hepatocyte growth factor-regulated tyrosine kinase substrate Hgs Hrs protein protein mouse Mus musculus Mouse MGRGSGTFERLLDKATSQLLLETDWESILQICDLIRQGDTQAKYAVNSIKKKVNDKNPHVALYALEVMESVVKNCGQTVHDEVANKQTMEELKELLKRQVEVNVRNKILYLIQAWAHAFRNEPKYKVVQDTYQIMKVEGHVFPEFKESDAMFAAERAPDWVDAEECHRCRVQFGVVTRKHHCRACGQIFCGKCSSKYSTIPKFGIEKEVRVCEPCYEQLNKKAEGKASSTTELPPEYLTSPLSQQSQLPPKRDETALQEEEELQLALALSQSEAEEKERMRQKTTYTAHPKAEPTPLASSAPPAGSLYSSPVNSSAPLAEDIDPELARYLNRNYWEKKQEEARKSPTPSAPVPLTEPAAQPGEGHTAPNSMAEAPLPETDSQPITPCSGPFSEYQNGESEESHEQFLKALQNAVSTFVNRMKSNHMRGRSITNDSAVLSLFQSINTMHPQLLELLNQLDERRLYYEGLQDKLAQIRDARGALSALREEHREKLRRAAEEAERQRQIQLAQKLEIMRQKKQEYLEVQRQLAIQRLQEQEKERQMRLEQQKQTVQMRAQMPAFPLPYAQLQAMPTAGGVLYQPSGPTSFPATFSPAGSVEGSPMHGVYMSQPAPATGPYPSMPGTTADPSMVSAYMYPTGAPGAQAAPQAQAGPTTSPAYSSYQPTPTPGYQSVASQAPQSLPAISQPPQTSNIGYMGSQPMSMGYQPYNMQNLMTALPGQDASLPAQQPYIPGQQPLYQQMAPSTGPPQQQPPVAQPAPTQGPPAQGSEAQLISFD q92783-1 Signal transducing adapter molecule 1 STAM STAM1 protein protein human Homo sapiens Human MPLFATNPFDQDVEKATSEMNTAEDWGLILDICDKVGQSRTGPKDCLRSIMRRVNHKDPHVAMQALTLLGACVSNCGKIFHLEVCSRDFASEVSNVLNKGHPKVCEKLKALMVEWTDEFKNDPQLSLISAMIKNLKEQGVTFPAIGSQAAEQAKASPALVAKDPGTVANKKEEEDLAKAIELSLKEQRQQSTTLSTLYPSTSSLLTNHQHEGRKVRAIYDFEAAEDNELTFKAGEIITVLDDSDPNWWKGETHQGIGLFPSNFVTADLTAEPEMIKTEKKTVQFSDDVQVETIEPEPEPAFIDEDKMDQLLQMLQSTDPSDDQPDLPELLHLEAMCHQMGPLIDEKLEDIDRKHSELSELNVKVMEALSLYTKLMNEDPMYSMYAKLQNQPYYMQSSGVSGSQVYAGPPPSGAYLVAGNAQMSHLQSYSLPPEQLSSLSQAVVPPSANPALPSQQTQAAYPNTMVSSVQGNTYPSQAPVYSPPPAATAAAATADVTLYQNAGPNMPQVPNYNLTSSTLPQPGGSQQPPQPQQPYSQKALL ubiq_bovin Ubiquitin RPS27A UBA80 UBCEP1 UBA52 UBCEP2 UBB UBC protein protein bovin Bos taurus (Bovine) Bovine MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG hgs-stam-4 3185650 N/A 3185661 unspecified role unspecified role unspecified role unspecified role human Homo sapiens Human N/A 3185662 unspecified role unspecified role unspecified role unspecified role human Homo sapiens Human physical association physical association false false false hgs-stam-5 3185651 N/A 3185663 unspecified role unspecified role unspecified role unspecified role trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 3185664 unspecified role unspecified role unspecified role unspecified role trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction false false false hgs-stam-6 3185652 N/A 3185662 unspecified role unspecified role unspecified role unspecified role Region Region 398-514 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3185664 unspecified role unspecified role unspecified role unspecified role Region 282-387 Region 282-387 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false hgs-stam-7 3185653 N/A 3185662 unspecified role unspecified role unspecified role unspecified role Region 1-557 Region 1-557 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3185664 unspecified role unspecified role unspecified role unspecified role Region 10-390 Region 10-390 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false hgs-stam-8 3185654 N/A 3185662 unspecified role unspecified role unspecified role unspecified role Region 1-557 Region 1-557 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3185664 unspecified role unspecified role unspecified role unspecified role Region 10-390 Region 10-390 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false hgs-stam-9 3185655 N/A 3185663 unspecified role unspecified role unspecified role unspecified role trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 3185664 unspecified role unspecified role unspecified role unspecified role trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction false false false hgs-stam-10 3185656 N/A 3185663 unspecified role unspecified role unspecified role unspecified role trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 3185664 unspecified role unspecified role unspecified role unspecified role trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction false false false hgs-stam-11 3185657 N/A 3185662 unspecified role unspecified role unspecified role unspecified role Region 1-557 Region 1-557 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3185664 unspecified role unspecified role unspecified role unspecified role Region 10-390 Region 10-390 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false stam-ub-1 3185658 N/A 3185664 unspecified role unspecified role bait bait Region 1-143 Region 1-143 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli N/A 3185665 unspecified role unspecified role prey prey bovin Bos taurus (Bovine) Bovine direct interaction direct interaction false false false hgs-stam-12 3185659 N/A 3185662 unspecified role unspecified role unspecified role unspecified role Region 398-514 Region 398-514 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3185664 unspecified role unspecified role unspecified role unspecified role Region 282-387 Region 282-387 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false hgs-stam-13 3185660 N/A 3185663 unspecified role unspecified role unspecified role unspecified role trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 3185664 unspecified role unspecified role unspecified role unspecified role trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction false false false