DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ li-2009-4 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Li W., Tu D., Li L., Wollert T., Ghirlando R., Brunger AT., Ye Y. DIP original experiment: DIP-216503X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Mechanistic insights into active site-associated polyubiquitination by the ubiquitin-conjugating enzyme Ube2g2. Li W., Tu D., Li L., Wollert T., Ghirlando R., Brunger AT., Ye Y. Only protein-protein interactions imex curation li-2009-5 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Li W., Tu D., Li L., Wollert T., Ghirlando R., Brunger AT., Ye Y. DIP original experiment: DIP-216508X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Mechanistic insights into active site-associated polyubiquitination by the ubiquitin-conjugating enzyme Ube2g2. Li W., Tu D., Li L., Wollert T., Ghirlando R., Brunger AT., Ye Y. Only protein-protein interactions imex curation li-2009-6 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Li W., Tu D., Li L., Wollert T., Ghirlando R., Brunger AT., Ye Y. DIP original experiment: DIP-216505X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Mechanistic insights into active site-associated polyubiquitination by the ubiquitin-conjugating enzyme Ube2g2. Li W., Tu D., Li L., Wollert T., Ghirlando R., Brunger AT., Ye Y. Only protein-protein interactions imex curation li-2009-7 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Li W., Tu D., Li L., Wollert T., Ghirlando R., Brunger AT., Ye Y. DIP original experiment: DIP-216513X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Mechanistic insights into active site-associated polyubiquitination by the ubiquitin-conjugating enzyme Ube2g2. Li W., Tu D., Li L., Wollert T., Ghirlando R., Brunger AT., Ye Y. Only protein-protein interactions imex curation li-2009-8 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Li W., Tu D., Li L., Wollert T., Ghirlando R., Brunger AT., Ye Y. DIP original experiment: DIP-216507X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Mechanistic insights into active site-associated polyubiquitination by the ubiquitin-conjugating enzyme Ube2g2. Li W., Tu D., Li L., Wollert T., Ghirlando R., Brunger AT., Ye Y. Only protein-protein interactions imex curation li-2009-9 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Li W., Tu D., Li L., Wollert T., Ghirlando R., Brunger AT., Ye Y. DIP original experiment: DIP-216509X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Mechanistic insights into active site-associated polyubiquitination by the ubiquitin-conjugating enzyme Ube2g2. Li W., Tu D., Li L., Wollert T., Ghirlando R., Brunger AT., Ye Y. Only protein-protein interactions imex curation li-2009-10 in vitro In vitro comigration in sds comigration in sds page comigration in sds anti tag western anti tag western blot anti tag western Li W., Tu D., Li L., Wollert T., Ghirlando R., Brunger AT., Ye Y. DIP original experiment: DIP-216511X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Mechanistic insights into active site-associated polyubiquitination by the ubiquitin-conjugating enzyme Ube2g2. Li W., Tu D., Li L., Wollert T., Ghirlando R., Brunger AT., Ye Y. Only protein-protein interactions imex curation li-2009-11 in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Li W., Tu D., Li L., Wollert T., Ghirlando R., Brunger AT., Ye Y. DIP original experiment: DIP-216504X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Mechanistic insights into active site-associated polyubiquitination by the ubiquitin-conjugating enzyme Ube2g2. Li W., Tu D., Li L., Wollert T., Ghirlando R., Brunger AT., Ye Y. Only protein-protein interactions imex curation li-2009-12 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Li W., Tu D., Li L., Wollert T., Ghirlando R., Brunger AT., Ye Y. DIP original experiment: DIP-216506X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Mechanistic insights into active site-associated polyubiquitination by the ubiquitin-conjugating enzyme Ube2g2. Li W., Tu D., Li L., Wollert T., Ghirlando R., Brunger AT., Ye Y. Only protein-protein interactions imex curation li-2009-13 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Li W., Tu D., Li L., Wollert T., Ghirlando R., Brunger AT., Ye Y. DIP original experiment: DIP-216512X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Mechanistic insights into active site-associated polyubiquitination by the ubiquitin-conjugating enzyme Ube2g2. Li W., Tu D., Li L., Wollert T., Ghirlando R., Brunger AT., Ye Y. Only protein-protein interactions imex curation li-2009-14 in vitro In vitro comigration in sds comigration in sds page comigration in sds anti tag western anti tag western blot anti tag western Li W., Tu D., Li L., Wollert T., Ghirlando R., Brunger AT., Ye Y. DIP original experiment: DIP-216510X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Mechanistic insights into active site-associated polyubiquitination by the ubiquitin-conjugating enzyme Ube2g2. Li W., Tu D., Li L., Wollert T., Ghirlando R., Brunger AT., Ye Y. Only protein-protein interactions imex curation amfr_human E3 ubiquitin-protein ligase AMFR gp78 RING finger protein 45 AMFR RNF45 Autocrine motility factor receptor protein protein human Homo sapiens Human MPLLFLERFPWPSLRTYTGLSGLALLGTIISAYRALSQPEAGPGEPDQLTASLQPEPPAPARPSAGGPRARDVAQYLLSDSLFVWVLVNTACCVLMLVAKLIQCIVFGPLRVSERQHLKDKFWNFIFYKFIFIFGVLNVQTVEEVVMWCLWFAGLVFLHLMVQLCKDRFEYLSFSPTTPMSSHGRVLSLLVAMLLSCCGLAAVCSITGYTHGMHTLAFMAAESLLVTVRTAHVILRYVIHLWDLNHEGTWEGKGTYVYYTDFVMELTLLSLDLMHHIHMLLFGNIWLSMASLVIFMQLRYLFHEVQRRIRRHKNYLRVVGNMEARFAVATPEELAVNNDDCAICWDSMQAARKLPCGHLFHNSCLRSWLEQDTSCPTCRMSLNIADNNRVREEHQGENLDENLVPVAAAEGRPRLNQHNHFFHFDGSRIASWLPSFSVEVMHTTNILGITQASNSQLNAMAHQIQEMFPQVPYHLVLQDLQLTRSVEITTDNILEGRIQVPFPTQRSDSIRPALNSPVERPSSDQEEGETSAQTERVPLDLSPRLEETLDFGEVEVEPSEVEDFEARGSRFSKSADERQRMLVQRKDELLQQARKRFLNKSSEDDAASESFLPSEGASSDPVTLRRRMLAAAAERRLQKQQTS herp1_human Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 1 protein Methyl methanesulfonate (MMF)-inducible fragment protein 1 HERPUD1 KIAA0025 MIF1 HERP protein protein human Homo sapiens Human MESETEPEPVTLLVKSPNQRHRDLELSGDRGWSVGHLKAHLSRVYPERPRPEDQRLIYSGKLLLDHQCLRDLLPKQEKRHVLHLVCNVKSPSKMPEINAKVAESTEEPAGSNRGQYPEDSSSDGLRQREVLRNLSSPGWENISRPEAAQQAFQGLGPGFSGYTPYGWLQLSWFQQIYARQYYMQYLAATAASGAFVPPPSAQEIPVVSAPAPAPIHNQFPAENQPANQNAAPQVVVNPGANQNLRMNAQGGPIVEEDDEINRDWLDWTYSAATFSVFLSILYFYSSLSRFLMVMGATVVMYLHHVGWFPFRPRPVQNFPNDGPPPDVVNQDPNNNLQEGTDPETEDPNHLPPDRDVLDGEQTSPSFMSTAWLVFKTFFASLLPEGPPAIAN ub2g2_mouse Ubiquitin-conjugating enzyme E2 G2 Ube2g2 Ubiquitin carrier protein G2 Ubiquitin-protein ligase G2 Ubc7 E2 ubiquitin-conjugating enzyme G2 protein protein mouse Mus musculus Mouse MAGTALKRLMAEYKQLTLNPPEGIVAGPMNEENFFEWEALIMGPEDTCFEFGVFPAILSFPLDYPLSPPKMRFTCEMFHPNIYPDGRVCISILHAPGDDPMGYESSAERWSPVQSVEKILLSVVSMLAEPNDESGANVDASKMWRDDREQFYKIAKQIVQKSLGL ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG amfr-amfr-12 3184418 N/A 3184429 unspecified role unspecified role prey prey Flag tag Flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human Homo sapiens Human N/A 3184429 unspecified role unspecified role bait bait GFP tag GFP tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag predetermined featur predetermined feature predetermined featur c-term range C-terminal range c-term range c-term range C-terminal range c-term range false 1-309 binding domain 1-309 binding domain sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false 379-456 binding domain 379-456 binding domain sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human physical association physical association false false false amfr-1 3184419 N/A 3184429 unspecified role unspecified role unspecified role unspecified role 309-643 binding domain 309-643 binding domain sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false amfr-herpud1-1 3184420 N/A 3184430 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3184429 unspecified role unspecified role bait bait GST tag GST tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range false 309-643 binding domain 309-643 binding domain sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false amfr-ube2g2-12 3184421 N/A 3184431 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3184429 unspecified role unspecified role unspecified role unspecified role 574-601 binding domain 574-601 binding domain sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false amfr-ube2g2-13 3184422 N/A 3184429 unspecified role unspecified role bait bait GST tag GST tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range false 309-643 binding domain 309-643 binding domain sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3184431 unspecified role unspecified role prey prey P60605:p.[Met42Ala;Phe51Ala;Leu163Ala;Leu165Ala] M42A F51A L163A L165A mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false ube2g2-ub-1 3184423 N/A 3184432 unspecified role unspecified role prey prey Flag tag Flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged predetermined featur predetermined feature predetermined featur n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false ecolx Escherichia coli N/A 3184431 unspecified role unspecified role bait bait ecolx Escherichia coli physical association physical association false false false herpud1-ub-2 3184424 N/A 3184430 unspecified role unspecified role unspecified role unspecified role His Tag His Tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3184432 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli covalent binding covalent binding false false false ube2g2-amfr-3 3184425 N/A 3184431 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3184429 unspecified role unspecified role prey prey 309-643 binding domain 309-643 binding domain sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3184431 unspecified role unspecified role bait bait Flag tag Flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli association association false false false amfr-ube2g2-14 3184426 N/A 3184431 unspecified role unspecified role prey prey P60605:p.[Leu163Ala;Leu165Ala] L163A L165A mutation mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P60605:p.[Met42Ala;Phe51Ala;Leu163Ala;Leu165Ala] M42A F51A L163A L165A mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3184429 unspecified role unspecified role bait bait GST tag GST tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range false 309-643 binding domain 309-643 binding domain sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false amfr-ube2g2-15 3184427 N/A 3184431 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3184429 unspecified role unspecified role unspecified role unspecified role 574-601 binding domain 574-601 binding domain sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false ub-ube2g2-3 3184428 N/A 3184432 unspecified role unspecified role unspecified role unspecified role Flag tag Flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged predetermined featur predetermined feature predetermined featur n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false ecolx Escherichia coli N/A 3184431 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli covalent binding covalent binding false false false