DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ barry-2009-2 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Barry ER., Lovett JE., Costa A., Lea SM., Bell SD. DIP original experiment: DIP-216485X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Intersubunit allosteric communication mediated by a conserved loop in the MCM helicase. Barry ER., Lovett JE., Costa A., Lea SM., Bell SD. Only protein-protein interactions imex curation barry-2009-3 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Barry ER., Lovett JE., Costa A., Lea SM., Bell SD. DIP original experiment: DIP-216487X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Intersubunit allosteric communication mediated by a conserved loop in the MCM helicase. Barry ER., Lovett JE., Costa A., Lea SM., Bell SD. Only protein-protein interactions imex curation barry-2009-4 in vitro In vitro crosslink cross-linking study crosslink western blot western blot Immuno blot Immuno blot Barry ER., Lovett JE., Costa A., Lea SM., Bell SD. DIP original experiment: DIP-216486X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Intersubunit allosteric communication mediated by a conserved loop in the MCM helicase. Barry ER., Lovett JE., Costa A., Lea SM., Bell SD. Only protein-protein interactions imex curation mcm_sacs2 Minichromosome maintenance protein MCM MCM SSO0774 protein protein sulso Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) MEIPSKQIDYRDVFIEFLTTFKGNNNQNKYIERINELVAYRKKSLIIEFSDVLSFNENLAYEIINNTKIILPILEGALYDHILQLDPTYQRDIEKVHVRIVGIPRVIELRKIRSTDIGKLITIDGILVKVTPVKERIYKATYKHIHPDCMQEFEWPEDEEMPEVLEMPTICPKCGKPGQFRLIPEKTKLIDWQKAVIQERPEEVPSGQLPRQLEIILEDDLVDSARPGDRVKVTGILDIKQDSPVKRGSRAVFDIYMKVSSIEVSQKVLDEVIISEEDEKKIKDLAKDPWIRDRIISSIAPSIYGHWELKEALALALFGGVPKVLEDTRIRGDIHILIIGDPGTAKSQMLQFISRVAPRAVYTTGKGSTAAGLTAAVVREKGTGEYYLEAGALVLADGGIAVIDEIDKMRDEDRVAIHEAMEQQTVSIAKAGIVAKLNARAAVIAAGNPKFGRYISERPVSDNINLPPTILSRFDLIFILKDQPGEQDRELANYILDVHSGKSTKNIIDIDTLRKYIAYARKYVTPKITSEAKNLITDFFVEMRKKSSETPDSPILITPRQLEALIRISEAYAKMALKAEVTREDAERAINIMRLFLESVGVDMESGKIDIDTIMTGKPKSAREKMMKIIEIIDSLAVSSECAKVKDILKEAQQVGIEKSNIEKLLTDMRKSGIIYEAKPECYKKV mint Has ATPase and DNA helicase activities. The latter preferentially melts 5'-tailed oligonucleotides and is stimulated by the SSB protein (single-stranded DNA binding protein) mcm-mcm-1 3183097 N/A 3183100 unspecified role unspecified role prey prey 267-686 binding site 267-686 binding site sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3183100 unspecified role unspecified role bait bait GST Tag GST Tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range false 267-686 binding site 267-686 binding site sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false mcm-6 3183098 N/A 3183100 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false mcm-mcm-2 3183099 N/A 3183100 unspecified role unspecified role unspecified role unspecified role Q9UXG1:p.Lys430Cys K430C mutation mutation mutation predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3183100 unspecified role unspecified role unspecified role unspecified role Q9UXG1:p.Ser206Cys S206C mutation mutation mutation predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false