DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ orabona-2008-1 mouse Mus musculus Mouse anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Orabona C., Pallotta MT., Volpi C., Fallarino F., Vacca C., Bianchi R., Belladonna ML., Fioretti MC., Grohmann U., Puccetti P. DIP original experiment: DIP-133598X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx SOCS3 drives proteasomal degradation of indoleamine 2,3-dioxygenase (IDO) and antagonizes IDO-dependent tolerogenesis. Orabona C., Pallotta MT., Volpi C., Fallarino F., Vacca C., Bianchi R., Belladonna ML., Fioretti MC., Grohmann U., Puccetti P. Only protein-protein interactions imex curation orabona-2008-2 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Orabona C., Pallotta MT., Volpi C., Fallarino F., Vacca C., Bianchi R., Belladonna ML., Fioretti MC., Grohmann U., Puccetti P. DIP original experiment: DIP-133596X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx SOCS3 drives proteasomal degradation of indoleamine 2,3-dioxygenase (IDO) and antagonizes IDO-dependent tolerogenesis. Orabona C., Pallotta MT., Volpi C., Fallarino F., Vacca C., Bianchi R., Belladonna ML., Fioretti MC., Grohmann U., Puccetti P. Only protein-protein interactions imex curation orabona-2008-3 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Orabona C., Pallotta MT., Volpi C., Fallarino F., Vacca C., Bianchi R., Belladonna ML., Fioretti MC., Grohmann U., Puccetti P. DIP original experiment: DIP-133599X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx SOCS3 drives proteasomal degradation of indoleamine 2,3-dioxygenase (IDO) and antagonizes IDO-dependent tolerogenesis. Orabona C., Pallotta MT., Volpi C., Fallarino F., Vacca C., Bianchi R., Belladonna ML., Fioretti MC., Grohmann U., Puccetti P. Only protein-protein interactions imex curation orabona-2008-4 mouse Mus musculus Mouse anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Orabona C., Pallotta MT., Volpi C., Fallarino F., Vacca C., Bianchi R., Belladonna ML., Fioretti MC., Grohmann U., Puccetti P. DIP original experiment: DIP-133597X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx SOCS3 drives proteasomal degradation of indoleamine 2,3-dioxygenase (IDO) and antagonizes IDO-dependent tolerogenesis. Orabona C., Pallotta MT., Volpi C., Fallarino F., Vacca C., Bianchi R., Belladonna ML., Fioretti MC., Grohmann U., Puccetti P. Only protein-protein interactions imex curation ubiq_mouse Ubiquitin Rps27a Uba80 Ubcep1 Uba52 Ubcep2 Ubb Ubc protein protein mouse Mus musculus Mouse MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG i23o1_mouse Indoleamine 2,3-dioxygenase 1 Ido1 Ido Indo Indoleamine-pyrrole 2,3-dioxygenase protein protein mouse Mus musculus Mouse MALSKISPTEGSRRILEDHHIDEDVGFALPHPLVELPDAYSPWVLVARNLPVLIENGQLREEVEKLPTLSTDGLRGHRLQRLAHLALGYITMAYVWNRGDDDVRKVLPRNIAVPYCELSEKLGLPPILSYADCVLANWKKKDPNGPMTYENMDILFSFPGGDCDKGFFLVSLLVEIAASPAIKAIPTVSSAVERQDLKALEKALHDIATSLEKAKEIFKRMRDFVDPDTFFHVLRIYLSGWKCSSKLPEGLLYEGVWDTPKMFSGGSAGQSSIFQSLDVLLGIKHEAGKESPAEFLQEMREYMPPAHRNFLFFLESAPPVREFVISRHNEDLTKAYNECVNGLVSVRKFHLAIVDTYIMKPSKKKPTDGDKSEEPSNVESRGTGGTNPMTFLRSVKDTTEKALLSWP itim1 ITIM1 peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) SGSGNIAVPYCELSE socs3_mouse Suppressor of cytokine signaling 3 Cytokine-inducible SH2 protein 3 Protein EF-10 Cis3 Cish3 Socs3 protein protein mouse Mus musculus Mouse MVTHSKFPAAGMSRPLDTSLRLKTFSSKSEYQLVVNAVRKLQESGFYWSAVTGGEANLLLSAEPAGTFLIRDSSDQRHFFTLSVKTQSGTKNLRIQCEGGSFSLQSDPRSTQPVPRFDCVLKLVHHYMPPPGTPSFSLPPTEPSSEVPEQPPAQALPGSTPKRAYYIYSGGEKIPLVLSRPLSSNVATLQHLCRKTVNGHLDSYEKVTQLPGPIREFLDQYDAPL itim2 ITIM2 peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) SGSGPEGLLYEGVWD ido1-ub-1 3181775 N/A 3181779 unspecified role unspecified role prey prey mouse Mus musculus Mouse N/A 3181780 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false mouse Mus musculus Mouse physical association physical association false false false itim1-socs3-1 3181776 N/A 3181781 unspecified role unspecified role bait bait pY10 pY10 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain false Biotin-tag Biotin-tag biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro N/A 3181782 unspecified role unspecified role prey prey mouse Mus musculus Mouse physical association physical association false false false itim2-socs3-1 3181777 N/A 3181783 unspecified role unspecified role bait bait pY10 pY10 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain false Biotin-tag Biotin-tag biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro N/A 3181782 unspecified role unspecified role prey prey mouse Mus musculus Mouse physical association physical association false false false ido1-socs3-1 3181778 N/A 3181782 unspecified role unspecified role prey prey mouse Mus musculus Mouse N/A 3181780 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false P28776:p.[Tyr115Phe;Tyr253Phe] Y115F/Y253F mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false mouse Mus musculus Mouse physical association physical association false false false