DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ murase-2008-1 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Murase K., Hirano Y., Sun TP., Hakoshima T. DIP original experiment: DIP-249985X Nature 2008 IMEx Gibberellin-induced DELLA recognition by the gibberellin receptor GID1. Murase K., Hirano Y., Sun TP., Hakoshima T. Only protein-protein interactions imex curation murase-2008-2 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Murase K., Hirano Y., Sun TP., Hakoshima T. DIP original experiment: DIP-249986X Nature 2008 IMEx Gibberellin-induced DELLA recognition by the gibberellin receptor GID1. Murase K., Hirano Y., Sun TP., Hakoshima T. Only protein-protein interactions imex curation murase-2008-3 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Murase K., Hirano Y., Sun TP., Hakoshima T. DIP original experiment: DIP-249987X Nature 2008 IMEx Gibberellin-induced DELLA recognition by the gibberellin receptor GID1. Murase K., Hirano Y., Sun TP., Hakoshima T. Only protein-protein interactions imex curation gai_arath DELLA protein GAI GAI RGA2 At1g14920 F10B6.34 Gibberellic acid-insensitive mutant protein Restoration of growth on ammonia protein 2 GRAS family protein 3 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MKRDHHHHHHQDKKTMMMNEEDDGNGMDELLAVLGYKVRSSEMADVAQKLEQLEVMMSNVQEDDLSQLATETVHYNPAELYTWLDSMLTDLNPPSSNAEYDLKAIPGDAILNQFAIDSASSSNQGGGGDTYTTNKRLKCSNGVVETTTATAESTRHVVLVDSQENGVRLVHALLACAEAVQKENLTVAEALVKQIGFLAVSQIGAMRKVATYFAEALARRIYRLSPSQSPIDHSLSDTLQMHFYETCPYLKFAHFTANQAILEAFQGKKRVHVIDFSMSQGLQWPALMQALALRPGGPPVFRLTGIGPPAPDNFDYLHEVGCKLAHLAEAIHVEFEYRGFVANTLADLDASMLELRPSEIESVAVNSVFELHKLLGRPGAIDKVLGVVNQIKPEIFTVVEQESNHNSPIFLDRFTESLHYYSTLFDSLEGVPSGQDKVMSEVYLGKQICNVVACDGPDRVERHETLSQWRNRFGSAGFAAAHIGSNAFKQASMLLALFNGGEGYRVEESDGCLMLGWHTRPLIATSAWKLSTN gid1a_arath Gibberellin receptor GID1A GID1A At3g05120 T12H1.8 GID1-like protein 1 CXE10 GID1L1 AtCXE10 Carboxylesterase 10 Protein GA INSENSITIVE DWARF 1A protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAASDEVNLIESRTVVPLNTWVLISNFKVAYNILRRPDGTFNRHLAEYLDRKVTANANPVDGVFSFDVLIDRRINLLSRVYRPAYADQEQPPSILDLEKPVDGDIVPVILFFHGGSFAHSSANSAIYDTLCRRLVGLCKCVVVSVNYRRAPENPYPCAYDDGWIALNWVNSRSWLKSKKDSKVHIFLAGDSSGGNIAHNVALRAGESGIDVLGNILLNPMFGGNERTESEKSLDGKYFVTVRDRDWYWKAFLPEGEDREHPACNPFSPRGKSLEGVSFPKSLVVVAGLDLIRDWQLAYAEGLKKAGQEVKLMHLEKATVGFYLLPNNNHFHNVMDEISAFVNAEC gai-gid1a-1 3180728 N/A 3180731 unspecified role unspecified role unspecified role unspecified role Region 11-113 Region 11-113 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3180732 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false gai-gid1a-2 3180729 N/A 3180731 unspecified role unspecified role unspecified role unspecified role Region 11-113 Region 11-113 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3180732 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false gid1a-gai-3 3180730 N/A 3180731 unspecified role unspecified role prey prey Region 11-113 Region 11-113 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Q9LQT8:p.[Leu31Ala;Val38Ala] L31A/V38A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q9LQT8:p.[Tyr36Ala;Met43Ala] Y36A/M43A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q9LQT8:p.[Glu51Arg;Glu54Arg] E51R/E54R mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q9LQT8:p.Tyr36Ala Y36A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3180732 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false