DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ koh-2008-1 in vitro In vitro peptide array peptide array anti tag western anti tag western blot anti tag western Koh AS., Kuo AJ., Park SY., Cheung P., Abramson J., Bua D., Carney D., Shoelson SE., Gozani O., Kingston RE., Benoist C., Mathis D. DIP original experiment: DIP-133393X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Aire employs a histone-binding module to mediate immunological tolerance, linking chromatin regulation with organ-specific autoimmunity. Koh AS., Kuo AJ., Park SY., Cheung P., Abramson J., Bua D., Carney D., Shoelson SE., Gozani O., Kingston RE., Benoist C., Mathis D. Only protein-protein interactions imex curation koh-2008-2 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Koh AS., Kuo AJ., Park SY., Cheung P., Abramson J., Bua D., Carney D., Shoelson SE., Gozani O., Kingston RE., Benoist C., Mathis D. DIP original experiment: DIP-133395X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Aire employs a histone-binding module to mediate immunological tolerance, linking chromatin regulation with organ-specific autoimmunity. Koh AS., Kuo AJ., Park SY., Cheung P., Abramson J., Bua D., Carney D., Shoelson SE., Gozani O., Kingston RE., Benoist C., Mathis D. Only protein-protein interactions imex curation koh-2008-3 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Koh AS., Kuo AJ., Park SY., Cheung P., Abramson J., Bua D., Carney D., Shoelson SE., Gozani O., Kingston RE., Benoist C., Mathis D. DIP original experiment: DIP-133394X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Aire employs a histone-binding module to mediate immunological tolerance, linking chromatin regulation with organ-specific autoimmunity. Koh AS., Kuo AJ., Park SY., Cheung P., Abramson J., Bua D., Carney D., Shoelson SE., Gozani O., Kingston RE., Benoist C., Mathis D. Only protein-protein interactions imex curation q9z0e3-1 Autoimmune regulator Aire Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein homolog protein protein mouse Mus musculus Mouse MAGGDGMLRRLLRLHRTEIAVAIDSAFPLLHALADHDVVPEDKFQETLRLKEKEGCPQAFHALLSWLLTRDSGAILDFWRILFKDYNLERYSRLHSILDGFPKDVDLNQSRKGRKPLAGPKAAVLPPRPPTKRKALEEPRATPPATLASKSVSSPGSHLKTKPPKKPDGNLESQHLPLGNGIQTMAASVQRAVTVASGDVPGTRGAVEGILIQQVFESGRSKKCIQVGGEFYTPNKFEDPSGNLKNKARSGSSLKPVVRAKGAQVTIPGRDEQKVGQQCGVPPLPSLPSEPQVNQKNEDECAVCHDGGELICCDGCPRAFHLACLSPPLQEIPSGLWRCSCCLQGRVQQNLSQPEVSRPPELPAETPILVGLRSASEKTRGPSRELKASSDAAVTYVNLLAPHPAAPLLEPSALCPLLSAGNEGRPGPAPSARCSVCGDGTEVLRCAHCAAAFHWRCHFPTAAARPGTNLRCKSCSADSTPTPGTPGEAVPTSGPRPAPGLAKVGDDSASHDPVLHRDDLESLLNEHSFDGILQWAIQSMSRPLAETPPFSS h3k9-2 Histone H3 N-terminal Peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) ARTKQTARKSTGGKAPRKQLA h3(121)-aire-1 3178249 N/A 3178252 unspecified role unspecified role prey prey Region 294-350 Region 294-350 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli N/A 3178253 unspecified role unspecified role bait bait Biotin-tag Biotin-tag biotin tag biotin tag c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal false in vitro In vitro direct interaction direct interaction false false false aire-h3(121)-1 3178250 N/A 3178253 unspecified role unspecified role unspecified role unspecified role in vitro In vitro N/A 3178252 unspecified role unspecified role unspecified role unspecified role Region 294-350 Region 294-350 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Q9Z0E3-1:p.Asp299Ala D299A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9Z0E3-1:p.Cys312Trp C312W mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9Z0E3-1:p.Pro328Leu P328L mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false h3(121)-aire-2 3178251 N/A 3178253 unspecified role unspecified role bait bait Biotin-tag Biotin-tag biotin tag biotin tag c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal false in vitro In vitro N/A 3178252 unspecified role unspecified role prey prey Region 294-350 Region 294-350 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Q9Z0E3-1:p.Cys312Trp C312W mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9Z0E3-1:p.Asp299Ala D299A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9Z0E3-1:p.Pro328Leu P328L mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli direct interaction direct interaction false false false