DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ mclellan-2008-1 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined McLellan JS., Zheng X., Hauk G., Ghirlando R., Beachy PA., Leahy DJ. DIP original experiment: DIP-250191X Nature 2008 IMEx The mode of Hedgehog binding to Ihog homologues is not conserved across different phyla. McLellan JS., Zheng X., Hauk G., Ghirlando R., Beachy PA., Leahy DJ. Only protein-protein interactions imex curation mclellan-2008-2 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined McLellan JS., Zheng X., Hauk G., Ghirlando R., Beachy PA., Leahy DJ. DIP original experiment: DIP-250193X Nature 2008 IMEx The mode of Hedgehog binding to Ihog homologues is not conserved across different phyla. McLellan JS., Zheng X., Hauk G., Ghirlando R., Beachy PA., Leahy DJ. Only protein-protein interactions imex curation mclellan-2008-3 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western McLellan JS., Zheng X., Hauk G., Ghirlando R., Beachy PA., Leahy DJ. DIP original experiment: DIP-250197X Nature 2008 IMEx The mode of Hedgehog binding to Ihog homologues is not conserved across different phyla. McLellan JS., Zheng X., Hauk G., Ghirlando R., Beachy PA., Leahy DJ. Only protein-protein interactions imex curation mclellan-2008-4 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined McLellan JS., Zheng X., Hauk G., Ghirlando R., Beachy PA., Leahy DJ. DIP original experiment: DIP-250189X Nature 2008 IMEx The mode of Hedgehog binding to Ihog homologues is not conserved across different phyla. McLellan JS., Zheng X., Hauk G., Ghirlando R., Beachy PA., Leahy DJ. Only protein-protein interactions imex curation mclellan-2008-5 in vitro In vitro pull down pull down predetermined predetermined participant predetermined McLellan JS., Zheng X., Hauk G., Ghirlando R., Beachy PA., Leahy DJ. DIP original experiment: DIP-250195X Nature 2008 IMEx The mode of Hedgehog binding to Ihog homologues is not conserved across different phyla. McLellan JS., Zheng X., Hauk G., Ghirlando R., Beachy PA., Leahy DJ. Only protein-protein interactions imex curation mclellan-2008-6 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined McLellan JS., Zheng X., Hauk G., Ghirlando R., Beachy PA., Leahy DJ. DIP original experiment: DIP-250198X Nature 2008 IMEx The mode of Hedgehog binding to Ihog homologues is not conserved across different phyla. McLellan JS., Zheng X., Hauk G., Ghirlando R., Beachy PA., Leahy DJ. Only protein-protein interactions imex curation mclellan-2008-7 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined McLellan JS., Zheng X., Hauk G., Ghirlando R., Beachy PA., Leahy DJ. DIP original experiment: DIP-250192X Nature 2008 IMEx The mode of Hedgehog binding to Ihog homologues is not conserved across different phyla. McLellan JS., Zheng X., Hauk G., Ghirlando R., Beachy PA., Leahy DJ. Only protein-protein interactions imex curation mclellan-2008-8 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined McLellan JS., Zheng X., Hauk G., Ghirlando R., Beachy PA., Leahy DJ. DIP original experiment: DIP-250194X Nature 2008 IMEx The mode of Hedgehog binding to Ihog homologues is not conserved across different phyla. McLellan JS., Zheng X., Hauk G., Ghirlando R., Beachy PA., Leahy DJ. Only protein-protein interactions imex curation mclellan-2008-9 in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined McLellan JS., Zheng X., Hauk G., Ghirlando R., Beachy PA., Leahy DJ. DIP original experiment: DIP-250190X Nature 2008 IMEx The mode of Hedgehog binding to Ihog homologues is not conserved across different phyla. McLellan JS., Zheng X., Hauk G., Ghirlando R., Beachy PA., Leahy DJ. Only protein-protein interactions imex curation mclellan-2008-10 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie McLellan JS., Zheng X., Hauk G., Ghirlando R., Beachy PA., Leahy DJ. DIP original experiment: DIP-250196X Nature 2008 IMEx The mode of Hedgehog binding to Ihog homologues is not conserved across different phyla. McLellan JS., Zheng X., Hauk G., Ghirlando R., Beachy PA., Leahy DJ. Only protein-protein interactions imex curation cdon_human Cell adhesion molecule-related/down-regulated by oncogenes CDON CDO protein protein human Homo sapiens Human MHPDLGPLCTLLYVTLTILCSSVSSDLAPYFTSEPLSAVQKLGGPVVLHCSAQPVTTRISWLHNGKTLDGNLEHVKIHQGTLTILSLNSSLLGYYQCLANNSIGAIVSGPATVSVAVLGDFGSSTKHVITAEEKSAGFIGCRVPESNPKAEVRYKIRGKWLEHSTENYLILPSGNLQILNVSLEDKGSYKCAAYNPVTHQLKVEPIGRKLLVSRPSSDDVHILHPTHSQALAVLSRSPVTLECVVSGVPAPQVYWLKDGQDIAPGSNWRRLYSHLATDSVDPADSGNYSCMAGNKSGDVKYVTYMVNVLEHASISKGLQDQIVSLGATVHFTCDVHGNPAPNCTWFHNAQPIHPSARHLTAGNGLKISGVTVEDVGMYQCVADNGIGFMHSTGRLEIENDGGFKPVIITAPVSAKVADGDFVTLSCNASGLPVPVIRWYDSHGLITSHPSQVLRSKSRKSQLSRPEGLNLEPVYFVLSQAGASSLHIQAVTQEHAGKYICEAANEHGTTQAEASLMVVPFETNTKAETVTLPDAAQNDDRSKRDGSETGLLSSFPVKVHPSAVESAPEKNASGISVPDAPIILSPPQTHTPDTYNLVWRAGKDGGLPINAYFVKYRKLDDGVGMLGSWHTVRVPGSENELHLAELEPSSLYEVLMVARSAAGEGQPAMLTFRTSKEKTASSKNTQASSPPVGIPKYPVVSEAANNNFGVVLTDSSRHSGVPEAPDRPTISTASETSVYVTWIPRANGGSPITAFKVEYKRMRTSNWLVAAEDIPPSKLSVEVRSLEPGSTYKFRVIAINHYGESFRSSASRPYQVVGFPNRFSSRPITGPHIAYTEAVSDTQIMLKWTYIPSSNNNTPIQGFYIYYRPTDSDNDSDYKRDVVEGSKQWHMIGHLQPETSYDIKMQCFNEGGESEFSNVMICETKVKRVPGASEYPVKDLSTPPNSLGSGGNVGPATSPARSSDMLYLIVGCVLGVMVLILMVFIAMCLWKNRQQNTIQKYDPPGYLYQGSDMNGQMVDYTTLSGASQINGNVHGGFLTNGGLSSGYSHLHHKVPNAVNGIVNGSLNGGLYSGHSNSLTRTHVDFEHPHHLVNGGGMYTAVPQIDPLECVNCRNCRNNNRCFTKTNSTFSSSPPPVVPVVAPYPQDGLEMKPLSHVKVPVCLTSAVPDCGQLPEESVKDNVEPVPTQRTCCQDIVNDVSSDGSEDPAEFSRGQEGMINLRIPDHLQLAKSCVWEGDSCAHSETEINIVSWNALILPPVPEGCAEKTMWSPPGIPLDSPTEVLQQPRET mint Component of a cell-surface receptor complex that mediates cell-cell interactions between muscle precursor cells. Promotes differentiation of myogenic cells shh_mouse Sonic hedgehog protein HHG-1 Shh Hhg1 Shh unprocessed N-terminal signaling and C-terminal autoprocessing domains protein protein mouse Mus musculus Mouse MLLLLARCFLVILASSLLVCPGLACGPGRGFGKRRHPKKLTPLAYKQFIPNVAEKTLGASGRYEGKITRNSERFKELTPNYNPDIIFKDEENTGADRLMTQRCKDKLNALAISVMNQWPGVKLRVTEGWDEDGHHSEESLHYEGRAVDITTSDRDRSKYGMLARLAVEAGFDWVYYESKAHIHCSVKAENSVAAKSGGCFPGSATVHLEQGGTKLVKDLRPGDRVLAADDQGRLLYSDFLTFLDRDEGAKKVFYVIETLEPRERLLLTAAHLLFVAPHNDSGPTPGPSALFASRVRPGQRVYVVAERGGDRRLLPAAVHSVTLREEEAGAYAPLTAHGTILINRVLASCYAVIEEHSWAHRAFAPFRLAHALLAALAPARTDGGGGGSIPAAQSATEARGAEPTAGIHWYSQLLYHIGTWLLDSETMHPLGMAVKSS ihog_drome Interference hedgehog ihog CG9211 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MTLLTSSLLLFSLLTSRLEAIPVLEKSPAHPAHSAHPAHPAHPAHPAHPSPGVRILRAPESLVAPLGDEVVLECETSLQPERFEWSHRSSRSPGAGFKYLKTGTAKANVSQEAAISRLRVLVRPDTLGEYRCVGWFGPLVVTSTIARLELASTSLVDAQESESPLQWRVSAGNSVLWSCGQQVQSNPSASWSYYRNGVEIKPEFIGTNGNLFLSNVSSESSGSYSCQATNPASGERIQLPGSLQLQVTPEQRSESKSPHLLRGQPSSQEITIREGSSLLLLCPGVGSPPPTVVWSSPDVVGAVKNKRSKVFGHALEISNTRVNDAGTYICFQDNGVRPALEHYIKVHVEQPPQIVRPPWADLTNEGDRLKLECKATGVPTPEIYWLLNGHSSIDDSEAELSNNFLILHSVLKRHAGYVQCFARNRLGEHSAGTLLQVNPKQIQEPRESGGTHRPKPNQGSRQKQMYPPTPPNVTRLSDESVMLRWMVPRNDGLPIVIFKVQYRMVGKRKNWQTTNDNIPYGKPKWNSELGKSFTASVTDLKPQHTYRFRILAVYSNNDNKESNTSAKFYLQPGAALDPMPVPELLEIEEYSETAVVLHWSLASDADEHLITGYYAYYRPSSSAGEYFKATIEGAHARSFKIAPLETATMYEFKLQSFSAASASEFSALKQGRTQRPKTSTTEEPTLQMGDRDTTTPSHNETFNMSPMLTGTIGGGAVLILLLISTCFCVCRRRNSRSRGNNPNKPRMAELRDDFVPLGNCSPTKQRQRTRHIHITLNPLAQQQQQALEEKNDTDQDAPYYQRPSSYDYDPTLRRMSSSSLRRSQRTLERAGGSNGSNNGNNNNLNQSAEAGSIENPGKPGRVLMKRPRLSSRSENLSSGSLNSVGV gas1_mouse Growth arrest-specific protein 1 Gas1 Gas-1 protein protein mouse Mus musculus Mouse MLAALLGGAGARTGTLPGALLCLMALLQLLCSAPRGSGLAHGRRLICWQALLQCQGEPDCSYAYSQYAEACAPVLAQRGGADAPGPAGAFPASAASSPRWRCPSHCISALIQLNHTRRGPALEDCDCAQDEHCRSTKRAIEPCLPRTSSVGPGAGAGSVMGCTEARRRCDRDSRCNLALSRYLAYCGKLFNGLRCTDECRAVIEDMLAVPKAALLNDCVCDGLERPICESVKENMARLCFGPDASNGPGSSGSDGGLDDYYDEEYDDEQRAGAAGGEQPLDDDDGLARPGGGAAAAGGRGDLPHGPGRRSSSSGSGGHWANRSAWTPFACLLLLLLLLLGSHL ihh_mouse Indian hedgehog protein Ihh HHG-2 protein protein mouse Mus musculus Mouse MSPAWLRPRLRFCLFLLLLLLVPAARGCGPGRVVGSRRRPPRKLVPLAYKQFSPNVPEKTLGASGRYEGKIARSSERFKELTPNYNPDIIFKDEENTGADRLMTQRCKDRLNSLAISVMNQWPGVKLRVTEGWDEDGHHSEESLHYEGRAVDITTSDRDRNKYGLLARLAVEAGFDWVYYESKAHVHCSVKSEHSAAAKTGGCFPAGAQVRLENGERVALSAVKPGDRVLAMGEDGTPTFSDVLIFLDREPNRLRAFQVIETQDPPRRLALTPAHLLFIADNHTEPAAHFRATFASHVQPGQYVLVSGVPGLQPARVAAVSTHVALGSYAPLTRHGTLVVEDVVASCFAAVADHHLAQLAFWPLRLFPSLAWGSWTPSEGVHWYPQMLYRLGRLLLEESTFHPLGMSGAGS q02936-1 Protein hedgehog hh CG4637 A B protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MDNHSSVPWASAASVTCLSLDAKCHSSSSSSSSKSAASSISAIPQEETQTMRHIAHTQRCLSRLTSLVALLLIVLPMVFSPAHSCGPGRGLGRHRARNLYPLVLKQTIPNLSEYTNSASGPLEGVIRRDSPKFKDLVPNYNRDILFRDEEGTGADRLMSKRCKEKLNVLAYSVMNEWPGIRLLVTESWDEDYHHGQESLHYEGRAVTIATSDRDQSKYGMLARLAVEAGFDWVSYVSRRHIYCSVKSDSSISSHVHGCFTPESTALLESGVRKPLGELSIGDRVLSMTANGQAVYSEVILFMDRNLEQMQNFVQLHTDGGAVLTVTPAHLVSVWQPESQKLTFVFADRIEEKNQVLVRDVETGELRPQRVVKVGSVRSKGVVAPLTREGTIVVNSVAASCYAVINSQSLAHWGLAPMRLLSTLEAWLPAKEQLHSSPKVVSSAQQQNGIHWYANALYKVKDYVLPQSWRHD cdon-shh-2 3177554 N/A 3177564 unspecified role unspecified role unspecified role unspecified role Region 826-924 Region 826-924 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3177565 unspecified role unspecified role unspecified role unspecified role Region 26-189 Region 26-189 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false ihog-shh-1 3177555 N/A 3177566 unspecified role unspecified role unspecified role unspecified role Region 466-679 Region 466-679 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3177565 unspecified role unspecified role unspecified role unspecified role Region 26-189 Region 26-189 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false cdon-shh-3 3177556 N/A 3177564 unspecified role unspecified role bait bait Region 826-924 Region 826-924 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Fc-tag Fc-tag fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecolx Escherichia coli N/A 3177565 unspecified role unspecified role prey prey Region 26-189 Region 26-189 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Q62226:p.Asp89Val D89V mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q62226:p.Glu127Lys E127K mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q62226:p.[Glu90Gln;Glu91Gln;Glu127Gln;Asp132Asn] E90Q/E91Q/E127Q/D132N mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q62226:p.[Lys88Ala;Arg124Ala;Arg154Ala;Ser178Ala] K88A/R124A/R154A/S178A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false cdon-shh-4 3177557 N/A 3177564 unspecified role unspecified role unspecified role unspecified role Region 826-924 Region 826-924 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3177565 unspecified role unspecified role unspecified role unspecified role Region 26-189 Region 26-189 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false gas1-shh-1 3177558 N/A 3177567 unspecified role unspecified role bait bait Fc-tag Fc-tag fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range false chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey N/A 3177565 unspecified role unspecified role prey prey Region 26-189 Region 26-189 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Q62226:p.Asp89Val D89V mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q62226:p.Glu127Lys E127K mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false cdon-ihh-1 3177559 N/A 3177564 unspecified role unspecified role unspecified role unspecified role Region 826-924 Region 826-924 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3177568 unspecified role unspecified role unspecified role unspecified role Region 67-231 Region 67-231 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false P97812:p.Glu131Lys E131K mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false cdon-shh-5 3177560 N/A 3177564 unspecified role unspecified role unspecified role unspecified role Region 826-924 Region 826-924 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3177565 unspecified role unspecified role unspecified role unspecified role Region 26-189 Region 26-189 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Q62226:p.Asp89Val D89V mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false hh-ihog-4 3177561 N/A 3177569 unspecified role unspecified role unspecified role unspecified role Region 86-248 Region 86-248 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3177566 unspecified role unspecified role unspecified role unspecified role Region 466-679 Region 466-679 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false cdon-shh-6 3177562 N/A 3177564 unspecified role unspecified role unspecified role unspecified role Region 826-924 Region 826-924 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3177565 unspecified role unspecified role unspecified role unspecified role Region 26-189 Region 26-189 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false cdon-shh-7 3177563 N/A 3177564 unspecified role unspecified role bait bait Region 722-927 Region 722-927 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3177565 unspecified role unspecified role prey prey Region 26-189 Region 26-189 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false