DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ hawse-2008-1 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Hawse WF., Hoff KG., Fatkins DG., Daines A., Zubkova OV., Schramm VL., Zheng W., Wolberger C. DIP original experiment: DIP-110832X Structure (London, England : 1993) 2008 IMEx Structural insights into intermediate steps in the Sir2 deacetylation reaction. Hawse WF., Hoff KG., Fatkins DG., Daines A., Zubkova OV., Schramm VL., Zheng W., Wolberger C. Only protein-protein interactions imex curation hawse-2008-2 in vitro In vitro deacetylase assay deacetylase assay predetermined predetermined participant predetermined Hawse WF., Hoff KG., Fatkins DG., Daines A., Zubkova OV., Schramm VL., Zheng W., Wolberger C. DIP original experiment: DIP-110833X Structure (London, England : 1993) 2008 IMEx Structural insights into intermediate steps in the Sir2 deacetylation reaction. Hawse WF., Hoff KG., Fatkins DG., Daines A., Zubkova OV., Schramm VL., Zheng W., Wolberger C. Only protein-protein interactions imex curation p53 peptide Acetyl-p53-peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) KKGQSTSRHKKLMFKTEG npd_thema NAD-dependent protein deacetylase cobB Regulatory protein SIR2 homolog sir2 TM_0490 protein protein thema Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099) MKMKEFLDLLNESRLTVTLTGAGISTPSGIPDFRGPNGIYKKYSQNVFDIDFFYSHPEEFYRFAKEGIFPMLQAKPNLAHVLLAKLEEKGLIEAVITQNIDRLHQRAGSKKVIELHGNVEEYYCVRCEKKYTVEDVIKKLESSDVPLCDDCNSLIRPNIVFFGENLPQDALREAIGLSSRASLMIVLGSSLVVYPAAELPLITVRSGGKLVIVNLGETPFDDIATLKYNMDVVEFARRVMEEGGIS 6BD2EF1B751C190D p53 peptide-sir2tm-1 3177336 N/A 3177338 unspecified role unspecified role unspecified role unspecified role in vitro In vitro N/A 3177339 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false p53 peptide-sir2tm-2 3177337 N/A 3177338 enzyme target enzyme target substrate substrate unspecified role unspecified role Acetylated-lysine Acetylated-lysine naclys N-acetylated L-lysine NAcLys certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3177339 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false