DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ ghazi_tabatabai-2008-1 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Ghazi-Tabatabai S., Saksena S., Short JM., Pobbati AV., Veprintsev DB., Crowther RA., Emr SD., Egelman EH., Williams RL. DIP original experiment: DIP-110827X Structure (London, England : 1993) 2008 IMEx Structure and disassembly of filaments formed by the ESCRT-III subunit Vps24. Ghazi-Tabatabai S., Saksena S., Short JM., Pobbati AV., Veprintsev DB., Crowther RA., Emr SD., Egelman EH., Williams RL. Only protein-protein interactions imex curation ghazi_tabatabai-2008-2 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Ghazi-Tabatabai S., Saksena S., Short JM., Pobbati AV., Veprintsev DB., Crowther RA., Emr SD., Egelman EH., Williams RL. DIP original experiment: DIP-110829X Structure (London, England : 1993) 2008 IMEx Structure and disassembly of filaments formed by the ESCRT-III subunit Vps24. Ghazi-Tabatabai S., Saksena S., Short JM., Pobbati AV., Veprintsev DB., Crowther RA., Emr SD., Egelman EH., Williams RL. Only protein-protein interactions imex curation ghazi_tabatabai-2008-3 in vitro In vitro 3d-em 3D electron microscopy 3D-EM electron tomog predetermined predetermined participant predetermined Ghazi-Tabatabai S., Saksena S., Short JM., Pobbati AV., Veprintsev DB., Crowther RA., Emr SD., Egelman EH., Williams RL. DIP original experiment: DIP-110828X Structure (London, England : 1993) 2008 IMEx Structure and disassembly of filaments formed by the ESCRT-III subunit Vps24. Ghazi-Tabatabai S., Saksena S., Short JM., Pobbati AV., Veprintsev DB., Crowther RA., Emr SD., Egelman EH., Williams RL. Only protein-protein interactions imex curation ghazi_tabatabai-2008-4 in vitro In vitro 3d-em 3D electron microscopy 3D-EM electron tomog predetermined predetermined participant predetermined Ghazi-Tabatabai S., Saksena S., Short JM., Pobbati AV., Veprintsev DB., Crowther RA., Emr SD., Egelman EH., Williams RL. DIP original experiment: DIP-110830X Structure (London, England : 1993) 2008 IMEx Structure and disassembly of filaments formed by the ESCRT-III subunit Vps24. Ghazi-Tabatabai S., Saksena S., Short JM., Pobbati AV., Veprintsev DB., Crowther RA., Emr SD., Egelman EH., Williams RL. Only protein-protein interactions imex curation ghazi_tabatabai-2008-5 in vitro In vitro 3d-em 3D electron microscopy 3D-EM electron tomog predetermined predetermined participant predetermined Ghazi-Tabatabai S., Saksena S., Short JM., Pobbati AV., Veprintsev DB., Crowther RA., Emr SD., Egelman EH., Williams RL. DIP original experiment: DIP-110831X Structure (London, England : 1993) 2008 IMEx Structure and disassembly of filaments formed by the ESCRT-III subunit Vps24. Ghazi-Tabatabai S., Saksena S., Short JM., Pobbati AV., Veprintsev DB., Crowther RA., Emr SD., Egelman EH., Williams RL. Only protein-protein interactions imex curation vps24_yeast Vacuolar protein-sorting-associated protein 24 YKL041W YKL254 VPS24 DID3 DOA4-independent degradation protein 3 ESCRT-III complex subunit VPS24 protein protein yeast Saccharomyces cerevisiae Baker's yeast MDYIKKAIWGPDPKEQQRRIRSVLRKNGRNIEKSLRELTVLQNKTQQLIKKSAKKNDVRTVRLYAKELYQINKQYDRMYTSRAQLDSVRMKIDEAIRMNTLSNQMADSAGLMREVNSLVRLPQLRNTMIELEKELMKSGIISEMVDDTMESVGDVGEEMDEAVDEEVNKIVEQYTNEKFKNVDQVPTVELAANEEEQEIPDEKVDEEADRMVNEMRERLRALQN 6073C1B680D58E98 snf7_yeast Vacuolar-sorting protein SNF7 SNF7 VPS32 YLR025W DID1 Vacuolar protein-sorting-associated protein 32 DOA4-independent degradation protein 1 Sucrose nonfermenting protein 7 protein protein yeast Saccharomyces cerevisiae Baker's yeast MWSSLFGWTSSNAKNKESPTKAIVRLREHINLLSKKQSHLRTQITNQENEARIFLTKGNKVMAKNALKKKKTIEQLLSKVEGTMESMEQQLFSIESANLNLETMRAMQEGAKAMKTIHSGLDIDKVDETMDEIREQVELGDEISDAISRPLITGANEVDEDELDEELDMLAQENANQETSKIVNNNVNAAPISENKVSLPSVPSNKIKQSENSVKDGEEEEDEEDEDEKALRELQAEMGL 5241A18BB181F0C1 vps24/vps2 VPS24-VPS2 Chimera protein protein yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast SQLQSTPQNLVSNAPIAETAMGIPEPIGAGSEFHGNPDDDLQARLNTLKKQTMDYIKKAIWGPDPKEQQRRIRSVLRKNGRNIEKSLRELTVLQNKTQQLIKKSAKKNDVRTVRLYAKELYQINKQYDRMYTSRAQLDSVRMKIDEAIRMNTLSNQMADSAGLMREVNSLVRLPQLRNTMIELEKELMKSGIISEMVDDTMESVGDVGEEMDEAVDEEVNKIVEQYTNEKF scvps24-1 3177311 N/A 3177316 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false scsnf7-1 3177312 N/A 3177317 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false scvps24-2 3177313 N/A 3177316 unspecified role unspecified role unspecified role unspecified role Region 1-9 Region 1-9 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false P36095:p.[Lys5Asp;Lys6Asp] K5D/K6D mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P36095:p.Lys55Ala K55A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P36095:p.[Lys133Asp;Glu134Ala;Lys137Asp] K133D/E134A/K137D mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P36095:p.[Arg120Glu;Leu124Asp;Lys133Asp] R120E/L124D/K133D mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P36095:p.[Leu124Asp;Arg125Glu] L124D/R125E mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P36095:p.[Arg120Glu;Leu121Asp] R120E/L121D mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false scsnf7-2 3177314 N/A 3177317 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false vps24/vps2-1 3177315 N/A 3177318 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false