DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ ding-2008-5 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Ding W., Li C., Hu T., Graves-Deal R., Fotia AB., Weissman AM., Coffey RJ. DIP original experiment: DIP-133241X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx EGF receptor-independent action of TGF-alpha protects Naked2 from AO7-mediated ubiquitylation and proteasomal degradation. Ding W., Li C., Hu T., Graves-Deal R., Fotia AB., Weissman AM., Coffey RJ. Only protein-protein interactions imex curation ding-2008-6 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Ding W., Li C., Hu T., Graves-Deal R., Fotia AB., Weissman AM., Coffey RJ. DIP original experiment: DIP-133244X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx EGF receptor-independent action of TGF-alpha protects Naked2 from AO7-mediated ubiquitylation and proteasomal degradation. Ding W., Li C., Hu T., Graves-Deal R., Fotia AB., Weissman AM., Coffey RJ. Only protein-protein interactions imex curation ding-2008-7 in vitro In vitro comigration in sds comigration in sds page comigration in sds anti tag western anti tag western blot anti tag western Ding W., Li C., Hu T., Graves-Deal R., Fotia AB., Weissman AM., Coffey RJ. DIP original experiment: DIP-133246X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx EGF receptor-independent action of TGF-alpha protects Naked2 from AO7-mediated ubiquitylation and proteasomal degradation. Ding W., Li C., Hu T., Graves-Deal R., Fotia AB., Weissman AM., Coffey RJ. Only protein-protein interactions imex curation ding-2008-8 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Ding W., Li C., Hu T., Graves-Deal R., Fotia AB., Weissman AM., Coffey RJ. DIP original experiment: DIP-133242X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx EGF receptor-independent action of TGF-alpha protects Naked2 from AO7-mediated ubiquitylation and proteasomal degradation. Ding W., Li C., Hu T., Graves-Deal R., Fotia AB., Weissman AM., Coffey RJ. Only protein-protein interactions imex curation ding-2008-9 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Ding W., Li C., Hu T., Graves-Deal R., Fotia AB., Weissman AM., Coffey RJ. DIP original experiment: DIP-133243X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx EGF receptor-independent action of TGF-alpha protects Naked2 from AO7-mediated ubiquitylation and proteasomal degradation. Ding W., Li C., Hu T., Graves-Deal R., Fotia AB., Weissman AM., Coffey RJ. Only protein-protein interactions imex curation ding-2008-10 human Homo sapiens Human comigration in sds comigration in sds page comigration in sds anti tag western anti tag western blot anti tag western Ding W., Li C., Hu T., Graves-Deal R., Fotia AB., Weissman AM., Coffey RJ. DIP original experiment: DIP-133245X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx EGF receptor-independent action of TGF-alpha protects Naked2 from AO7-mediated ubiquitylation and proteasomal degradation. Ding W., Li C., Hu T., Graves-Deal R., Fotia AB., Weissman AM., Coffey RJ. Only protein-protein interactions imex curation nkd2_human Protein naked cuticle homolog 2 NKD2 protein protein human Homo sapiens Human MGKLQSKHAAAARKRRESPEGDSFVASAYASGRKGAEEAERRARDKQELPNGDPKEGPFREDQCPLQVALPAEKAEGREHPGQLLSADDGERAANREGPRGPGGQRLNIDALQCDVSVEEDDRQEWTFTLYDFDNCGKVTREDMSSLMHTIYEVVDASVNHSSGSSKTLRVKLTVSPEPSSKRKEGPPAGQDREPTRCRMEGELAEEPRVADRRLSAHVRRPSTDPQPCSERGPYCVDENTERRNHYLDLAGIENYTSRFGPGSPPVQAKQEPQGRASHLQARSRSQEPDTHAVHHRRSQVLVEHVVPASEPAARALDTQPRPKGPEKQFLKSPKGSGKPPGVPASSKSGKAFSYYLPAVLPPQAPQDGHHLPQPPPPPYGHKRYRQKGREGHSPLKAPHAQPATVEHEVVRDLPPTPAGEGYAVPVIQRHEHHHHHEHHHHHHHHHFHPS ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG rnf25_human E3 ubiquitin-protein ligase RNF25 RNF25 RING finger protein 25 RING-type E3 ubiquitin transferase RNF25 protein protein human Homo sapiens Human MAASASAAAGEEDWVLPSEVEVLESIYLDELQVIKGNGRTSPWEIYITLHPATAEDQDSQYVCFTLVLQVPAEYPHEVPQISIRNPRGLSDEQIHTILQVLGHVAKAGLGTAMLYELIEKGKEILTDNNIPHGQCVICLYGFQEKEAFTKTPCYHYFHCHCLARYIQHMEQELKAQGQEQEQERQHATTKQKAVGVQCPVCREPLVYDLASLKAAPEPQQPMELYQPSAESLRQQEERKRLYQRQQERGGIIDLEAERNRYFISLQQPPAPAEPESAVDVSKGSQPPSTLAAELSTSPAVQSTLPPPLPVATQHICEKIPGTRSNQQRLGETQKAMLDPPKPSRGPWRQPERRHPKGGECHAPKGTRDTQELPPPEGPLKEPMDLKPEPHSQGVEGPPQEKGPGSWQGPPPRRTRDCVRWERSKGRTPGSSYPRLPRGQGAYRPGTRRESLGLESKDGS tgfa_human Protransforming growth factor alpha TGFA protein protein human Homo sapiens Human MVPSAGQLALFALGIVLAACQALENSTSPLSADPPVAAAVVSHFNDCPDSHTQFCFHGTCRFLVQEDKPACVCHSGYVGARCEHADLLAVVAASQKKQAITALVVVSIVALAVLIITCVLIHCCQVRKHCEWCRALICRHEKPSALLKGRTACCHSETVV nkd2-ub-1 3176985 N/A 3176991 unspecified role unspecified role bait bait human Homo sapiens Human N/A 3176992 unspecified role unspecified role prey prey Ha tag Ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag predetermined featur predetermined feature predetermined featur n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false human Homo sapiens Human physical association physical association false false false nkd2-rnf25-1 3176986 N/A 3176991 unspecified role unspecified role bait bait tag GFP tag GFP gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag predetermined featur predetermined feature predetermined featur c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human Homo sapiens Human N/A 3176993 unspecified role unspecified role prey prey Ha tag Ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false nkd2-ub-2 3176987 N/A 3176991 unspecified role unspecified role unspecified role unspecified role GFP tag GFP tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag predetermined featur predetermined feature predetermined featur c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human Homo sapiens Human N/A 3176992 unspecified role unspecified role unspecified role unspecified role Ha tag Ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag predetermined featur predetermined feature predetermined featur n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false human Homo sapiens Human covalent binding covalent binding false false false tgfa-nkd2-3 3176988 N/A 3176991 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3176994 unspecified role unspecified role bait bait Ha tag Ha tag experimental feature experimental feature predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false nkd2-rnf25-2 3176989 N/A 3176991 unspecified role unspecified role bait bait human Homo sapiens Human N/A 3176993 unspecified role unspecified role prey prey Ha tag Ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false nkd2-ub-3 3176990 N/A 3176991 unspecified role unspecified role unspecified role unspecified role human Homo sapiens Human N/A 3176992 unspecified role unspecified role unspecified role unspecified role Ha tag Ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag predetermined featur predetermined feature predetermined featur n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false human Homo sapiens Human covalent binding covalent binding false false false