DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ yamoah-2008-1 in vitro In vitro comigration in sds comigration in sds page comigration in sds autoradiography autoradiography Yamoah K., Oashi T., Sarikas A., Gazdoiu S., Osman R., Pan ZQ. DIP original experiment: DIP-133222X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Autoinhibitory regulation of SCF-mediated ubiquitination by human cullin 1's C-terminal tail. Yamoah K., Oashi T., Sarikas A., Gazdoiu S., Osman R., Pan ZQ. Only protein-protein interactions imex curation yamoah-2008-2 in vitro In vitro comigration in sds comigration in sds page comigration in sds western blot western blot Immuno blot Immuno blot Yamoah K., Oashi T., Sarikas A., Gazdoiu S., Osman R., Pan ZQ. DIP original experiment: DIP-133225X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Autoinhibitory regulation of SCF-mediated ubiquitination by human cullin 1's C-terminal tail. Yamoah K., Oashi T., Sarikas A., Gazdoiu S., Osman R., Pan ZQ. Only protein-protein interactions imex curation yamoah-2008-3 in vitro In vitro comigration in sds comigration in sds page comigration in sds autoradiography autoradiography Yamoah K., Oashi T., Sarikas A., Gazdoiu S., Osman R., Pan ZQ. DIP original experiment: DIP-133223X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Autoinhibitory regulation of SCF-mediated ubiquitination by human cullin 1's C-terminal tail. Yamoah K., Oashi T., Sarikas A., Gazdoiu S., Osman R., Pan ZQ. Only protein-protein interactions imex curation yamoah-2008-4 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Yamoah K., Oashi T., Sarikas A., Gazdoiu S., Osman R., Pan ZQ. DIP original experiment: DIP-133224X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Autoinhibitory regulation of SCF-mediated ubiquitination by human cullin 1's C-terminal tail. Yamoah K., Oashi T., Sarikas A., Gazdoiu S., Osman R., Pan ZQ. Only protein-protein interactions imex curation ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG ikba_human NF-kappa-B inhibitor alpha NFKBIA IKBA MAD3 NFKBI I-kappa-B-alpha Major histocompatibility complex enhancer-binding protein MAD3 protein protein human Homo sapiens Human MFQAAERPQEWAMEGPRDGLKKERLLDDRHDSGLDSMKDEEYEQMVKELQEIRLEPQEVPRGSEPWKQQLTEDGDSFLHLAIIHEEKALTMEVIRQVKGDLAFLNFQNNLQQTPLHLAVITNQPEIAEALLGAGCDPELRDFRGNTPLHLACEQGCLASVGVLTQSCTTPHLHSILKATNYNGHTCLHLASIHGYLGIVELLVSLGADVNAQEPCNGRTALHLAVDLQNPDLVSLLLKCGADVNRVTYQGYSPYQLTWGRPSTRIQQQLGQLTLENLQMLPESEDEESYDTESEFTEFTEDELPYDDCVFGGQRLTL nedd8_human NEDD8 Ubiquitin-like protein Nedd8 Neddylin Neural precursor cell expressed developmentally down-regulated protein 8 NEDD8 protein protein human Homo sapiens Human MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGGGGLRQ cul1_human Cullin-1 CUL1 protein protein human Homo sapiens Human MSSTRSQNPHGLKQIGLDQIWDDLRAGIQQVYTRQSMAKSRYMELYTHVYNYCTSVHQSNQARGAGVPPSKSKKGQTPGGAQFVGLELYKRLKEFLKNYLTNLLKDGEDLMDESVLKFYTQQWEDYRFSSKVLNGICAYLNRHWVRRECDEGRKGIYEIYSLALVTWRDCLFRPLNKQVTNAVLKLIEKERNGETINTRLISGVVQSYVELGLNEDDAFAKGPTLTVYKESFESQFLADTERFYTRESTEFLQQNPVTEYMKKAEARLLEEQRRVQVYLHESTQDELARKCEQVLIEKHLEIFHTEFQNLLDADKNEDLGRMYNLVSRIQDGLGELKKLLETHIHNQGLAAIEKCGEAALNDPKMYVQTVLDVHKKYNALVMSAFNNDAGFVAALDKACGRFINNNAVTKMAQSSSKSPELLARYCDSLLKKSSKNPEEAELEDTLNQVMVVFKYIEDKDVFQKFYAKMLAKRLVHQNSASDDAEASMISKLKQACGFEYTSKLQRMFQDIGVSKDLNEQFKKHLTNSEPLDLDFSIQVLSSGSWPFQQSCTFALPSELERSYQRFTAFYASRHSGRKLTWLYQLSKGELVTNCFKNRYTLQASTFQMAILLQYNTEDAYTVQQLTDSTQIKMDILAQVLQILLKSKLLVLEDENANVDEVELKPDTLIKLYLGYKNKKLRVNINVPMKTEQKQEQETTHKNIEEDRKLLIQAAIVRIMKMRKVLKHQQLLGEVLTQLSSRFKPRVPVIKKCIDILIEKEYLERVDGEKDTYSYLA ub2r1_human Ubiquitin-conjugating enzyme E2 R1 Ubiquitin-protein ligase R1 Ubiquitin-conjugating enzyme E2-32 kDa complementing Ubiquitin-conjugating enzyme E2-CDC34 CDC34 UBE2R1 UBCH3 (E3-independent) E2 ubiquitin-conjugating enzyme R1 E2 ubiquitin-conjugating enzyme R1 protein protein human Homo sapiens Human MARPLVPSSQKALLLELKGLQEEPVEGFRVTLVDEGDLYNWEVAIFGPPNTYYEGGYFKARLKFPIDYPYSPPAFRFLTKMWHPNIYETGDVCISILHPPVDDPQSGELPSERWNPTQNVRTILLSVISLLNEPNTFSPANVDASVMYRKWKESKGKDREYTDIIRKQVLGTKVDAERDGVKVPTTLAEYCVKTKAPAPDEGSDLFYDDYYEDGEVEEEADSCFGDDEDDSGTEES nfkbia-ub-2 3176577 N/A 3176581 unspecified role unspecified role unspecified role unspecified role human Homo sapiens Human N/A 3176582 unspecified role unspecified role unspecified role unspecified role GST tag GST tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli covalent binding covalent binding false false false cul1-nedd8-3 3176578 N/A 3176583 unspecified role unspecified role unspecified role unspecified role human Homo sapiens Human N/A 3176584 unspecified role unspecified role unspecified role unspecified role human Homo sapiens Human covalent binding covalent binding false false false cdc34-ub-1 3176579 N/A 3176581 unspecified role unspecified role unspecified role unspecified role human Homo sapiens Human N/A 3176585 unspecified role unspecified role unspecified role unspecified role human Homo sapiens Human covalent binding covalent binding false false false cul1-nedd8-4 3176580 N/A 3176583 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3176584 unspecified role unspecified role bait bait Flag tag Flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q13616:p.[Lys720Arg;Tyr761Ala] K720R/Y761A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human physical association physical association false false false