DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ ohtsubo-2008-1 spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm pull down pull down predetermined predetermined participant predetermined Ohtsubo M., Yasunaga S., Ohno Y., Tsumura M., Okada S., Ishikawa N., Shirao K., Kikuchi A., Nishitani H., Kobayashi M., Takihara Y. DIP original experiment: DIP-260418X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Polycomb-group complex 1 acts as an E3 ubiquitin ligase for Geminin to sustain hematopoietic stem cell activity. Ohtsubo M., Yasunaga S., Ohno Y., Tsumura M., Okada S., Ishikawa N., Shirao K., Kikuchi A., Nishitani H., Kobayashi M., Takihara Y. Only protein-protein interactions imex curation ohtsubo-2008-2 spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Ohtsubo M., Yasunaga S., Ohno Y., Tsumura M., Okada S., Ishikawa N., Shirao K., Kikuchi A., Nishitani H., Kobayashi M., Takihara Y. DIP original experiment: DIP-260421X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Polycomb-group complex 1 acts as an E3 ubiquitin ligase for Geminin to sustain hematopoietic stem cell activity. Ohtsubo M., Yasunaga S., Ohno Y., Tsumura M., Okada S., Ishikawa N., Shirao K., Kikuchi A., Nishitani H., Kobayashi M., Takihara Y. Only protein-protein interactions imex curation ohtsubo-2008-3 spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm pull down pull down predetermined predetermined participant predetermined Ohtsubo M., Yasunaga S., Ohno Y., Tsumura M., Okada S., Ishikawa N., Shirao K., Kikuchi A., Nishitani H., Kobayashi M., Takihara Y. DIP original experiment: DIP-260423X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Polycomb-group complex 1 acts as an E3 ubiquitin ligase for Geminin to sustain hematopoietic stem cell activity. Ohtsubo M., Yasunaga S., Ohno Y., Tsumura M., Okada S., Ishikawa N., Shirao K., Kikuchi A., Nishitani H., Kobayashi M., Takihara Y. Only protein-protein interactions imex curation ohtsubo-2008-4 spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm pull down pull down predetermined predetermined participant predetermined Ohtsubo M., Yasunaga S., Ohno Y., Tsumura M., Okada S., Ishikawa N., Shirao K., Kikuchi A., Nishitani H., Kobayashi M., Takihara Y. DIP original experiment: DIP-260425X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Polycomb-group complex 1 acts as an E3 ubiquitin ligase for Geminin to sustain hematopoietic stem cell activity. Ohtsubo M., Yasunaga S., Ohno Y., Tsumura M., Okada S., Ishikawa N., Shirao K., Kikuchi A., Nishitani H., Kobayashi M., Takihara Y. Only protein-protein interactions imex curation ohtsubo-2008-5 spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm pull down pull down predetermined predetermined participant predetermined Ohtsubo M., Yasunaga S., Ohno Y., Tsumura M., Okada S., Ishikawa N., Shirao K., Kikuchi A., Nishitani H., Kobayashi M., Takihara Y. DIP original experiment: DIP-260420X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Polycomb-group complex 1 acts as an E3 ubiquitin ligase for Geminin to sustain hematopoietic stem cell activity. Ohtsubo M., Yasunaga S., Ohno Y., Tsumura M., Okada S., Ishikawa N., Shirao K., Kikuchi A., Nishitani H., Kobayashi M., Takihara Y. Only protein-protein interactions imex curation ohtsubo-2008-6 spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Ohtsubo M., Yasunaga S., Ohno Y., Tsumura M., Okada S., Ishikawa N., Shirao K., Kikuchi A., Nishitani H., Kobayashi M., Takihara Y. DIP original experiment: DIP-260426X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Polycomb-group complex 1 acts as an E3 ubiquitin ligase for Geminin to sustain hematopoietic stem cell activity. Ohtsubo M., Yasunaga S., Ohno Y., Tsumura M., Okada S., Ishikawa N., Shirao K., Kikuchi A., Nishitani H., Kobayashi M., Takihara Y. Only protein-protein interactions imex curation ohtsubo-2008-7 spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Ohtsubo M., Yasunaga S., Ohno Y., Tsumura M., Okada S., Ishikawa N., Shirao K., Kikuchi A., Nishitani H., Kobayashi M., Takihara Y. DIP original experiment: DIP-260422X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Polycomb-group complex 1 acts as an E3 ubiquitin ligase for Geminin to sustain hematopoietic stem cell activity. Ohtsubo M., Yasunaga S., Ohno Y., Tsumura M., Okada S., Ishikawa N., Shirao K., Kikuchi A., Nishitani H., Kobayashi M., Takihara Y. Only protein-protein interactions imex curation ohtsubo-2008-8 in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Ohtsubo M., Yasunaga S., Ohno Y., Tsumura M., Okada S., Ishikawa N., Shirao K., Kikuchi A., Nishitani H., Kobayashi M., Takihara Y. DIP original experiment: DIP-260419X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Polycomb-group complex 1 acts as an E3 ubiquitin ligase for Geminin to sustain hematopoietic stem cell activity. Ohtsubo M., Yasunaga S., Ohno Y., Tsumura M., Okada S., Ishikawa N., Shirao K., Kikuchi A., Nishitani H., Kobayashi M., Takihara Y. Only protein-protein interactions imex curation ohtsubo-2008-9 spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Ohtsubo M., Yasunaga S., Ohno Y., Tsumura M., Okada S., Ishikawa N., Shirao K., Kikuchi A., Nishitani H., Kobayashi M., Takihara Y. DIP original experiment: DIP-260424X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Polycomb-group complex 1 acts as an E3 ubiquitin ligase for Geminin to sustain hematopoietic stem cell activity. Ohtsubo M., Yasunaga S., Ohno Y., Tsumura M., Okada S., Ishikawa N., Shirao K., Kikuchi A., Nishitani H., Kobayashi M., Takihara Y. Only protein-protein interactions imex curation q8k214-2 Polycomb protein SCMH1 Scmh1 Sex comb on midleg homolog 1 protein protein mouse Mus musculus Mouse MLVCYSVLACESLWDLPCSIMGSPLGHFTWDKYLKETCSVPAPVHCFKQSYTPPSNEFKISMKLEAQDPRNTTSTCIATVVGLTGARLRLRLDGSDNKNDFWRLVDSSEIQPIGNCEKNGGMLQPPLGFRLNASSWPMFLLKTLNGAEMAPIKIFHKEPPSPSHNFFKMGMKLEAVDRKNPHFICPATIGEVRGAEVLVTFDGWRGAFDYWCRFDSRDIFPVGWCSLTGDNLQPPGTKVVIPKNPSPSSDVSTEKPSIHSTKTVLEHQPGQRGRKPGKKRGRTPKILIPHPTSTPSKSAEPLKFPKKRGPKPGSKRKPRTLLSPPPTSPTTSTPEPDTSTVPQDAATVPSSAMQAPTVCIYLNKSGSTGPHLDKKKIQQLPDHFGPARASVVLQQAVQACIDCAYHQKTVFSFLKQGHGGEVISAVFDREQHTLNLPAVNSITYVLRFLEKLCHNLRSDNLFGNQPFTQTHLSLTATEYNHNHDRYLPGETFVLGNSLARSLETHSDLMDSALKPANLVSTSQNLRTPGYRPLLPSCGLPLSTVSAVRRLCSKGVLKGKKERRDVESFWKLNHSPGSDRHLESRDPPRLSGRDPSSWTVEDVMQFVREADPQLGSHADLFRKHEIDGKALLLLRSDMMMKYMGLKLGPALKLSFHIDRLKQGKF phc1_mouse Polyhomeotic-like protein 1 Phc1 Edr Edr1 Rae28 Early development regulatory protein 1 RAE-28 protein protein mouse Mus musculus Mouse METESEQNSSSTNGSSSSGASSRPQIAQMSLYERQAVQALQALQRQPNAAQYFHQFMLQQQLSNAQLHSLAAVQQATIAASRQASSPNSSTAQQQTATTQASMNLATTSAAQLISRSQSVSSPSATTLTQSVLLGNTTSPPLNQSQAQMYLRPQLGNLLQVNRTLGRNVPLASQLILMPNGAVAAVQQEVPPAQSPGVHADADQVQNLAVRNQQASAQGPQMPGSTQKAIPPGASPVSGLSQTSSQALAVAQASSGASGQSLNLSQAGGGSGNSLPGSMGPGGGGQAPGGLGQLPSSGLTGGSCPRKGTGVVQPLPAAQTVTVSQGSQTEAESAAAKKAEADGSGQQSVGMNLTRTATPAPSQTLISSATYTQIQPHSLIQQQQQIHLQQKQVVIQQQIAIHHQQQFQHRQSQLLHTATHLQLAQQQQQQQQQQQQQQQQQQQQQQQQGTTLTAPQPPQVPPTQQVPPSQSQQQAQTLVVQPMLQSSPLTLPPEPTSKPPIPIQSKPPVAPIKPPQLGAAKMSATQQPPPHIPVQVVGTRQPGSAQAQALGLAQLAAAVPTPRGITGAVQPGQAHLASSPPSSQAAPGALQECPPALAAGMTLAPVQGTAHVVKGGPTASSPVVAQVPAAFYMQSVHLPGKAQTLAVKRKAESEEERDDLSALASVLPTKASPAAESPKVIEEKNSLGEKAEPVASLNANPPNSDLVALAPTPSAPPPTLALVSRQMGDSKPPQAIVKPQILTHIIEGFVIQEGAEPFPVGCSQFLKETEKPLQAGLPTGLNESQPSGPLGGDSPSVELEKKANLLKCEYCGKYAPAEQFRGSKRFCSMTCAKRYNVSCSHQFRLKRKKMKEFQEASYARVRRRGPRRSSSDIARAKIQGKRHRGQEDSSRGSDNSSYDEALSPTSPGPLSVRAGHGERDLGNTITTPSTPELQGINPVFLSSNPSQWSVEEVYEFIASLQGCQEIAEEFRSQEIDGQALLLLKEEHLMSAMNIKLGPALKICAKINVLKET bmi1_mouse Polycomb complex protein BMI-1 Bmi1 Pcgf4 Bmi-1 Polycomb group RING finger protein 4 protein protein mouse Mus musculus Mouse MHRTTRIKITELNPHLMCVLCGGYFIDATTIIECLHSFCKTCIVRYLETSKYCPICDVQVHKTRPLLNIRSDKTLQDIVYKLVPGLFKNEMKRRRDFYAAHPSADAANGSNEDRGEVADEEKRIITDDEIISLSIEFFDQSRLDRKVNKEKPKEEVNDKRYLRCPAAMTVMHLRKFLRSKMDIPNTFQIDVMYEEEPLKDYYTLMDIAYIYTWRRNGPLPLKYRVRPTCKRMKMSHQRDGLTNAGELESDSGSDKANSPAGGVPSTSSCLPSPSTPVQSPHPQFPHISSTMNGTSNSPSANHQSSFASRPRKSSLNGSSATSSG ring2_mouse E3 ubiquitin-protein ligase RING2 Rnf2 DinG Ring1b RING finger protein 2 RING finger protein 1B RING-type E3 ubiquitin transferase RING2 protein protein mouse Mus musculus Mouse MSQAVQTNGTQPLSKTWELSLYELQRTPQEAITDGLEIVVSPRSLHSELMCPICLDMLKNTMTTKECLHRFCADCIITALRSGNKECPTCRKKLVSKRSLRPDPNFDALISKIYPSRDEYEAHQERVLARINKHNNQQALSHSIEEGLKIQAMNRLQRGKKQQIENGSGAEDNGDSSHCSNASTHSNQEAGPSNKRTKTSDDSGLELDNNNAAVAIDPVMDGASEIELVFRPHPTLMEKDDSAQTRYIKTSGNATVDHLSKYLAVRLALEELRSKGESNQMNLDTASEKQYTIYIATASGQFTVLNGSFSLELVSEKYWKVNKPMELYYAPTKEHK ubiq_mouse Ubiquitin Rps27a Uba80 Ubcep1 Uba52 Ubcep2 Ubb Ubc protein protein mouse Mus musculus Mouse MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG gemi_mouse Geminin Gmnn protein protein mouse Mus musculus Mouse MNLSMKQKQEGAQENVKNSPVPRRTLKMIQPSADGSLVGRENELPKGLFKRKLWDDQLASQTSSCGPEANENKDVGDLTQEAFDLISKENPSSQYWKEVAEQRRKALYEALKENEKLHKEIEQKDSEIARLRKENKDLAEVAEHVQYMAEVIERLSNEPLDNFESPDSQEFDSEEEAVEYSELEDSGAGTCAEETVSSSTDARPCT rnf2-bmi1-2 3174986 N/A 3174995 unspecified role unspecified role prey prey Region 358-664 Region 358-664 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3174996 unspecified role unspecified role prey prey Region 222-1012 Region 222-1012 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3174997 unspecified role unspecified role prey prey spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3174998 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm association association false false false bmi1-phc1-1 3174987 N/A 3174995 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3174996 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3174997 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3174998 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm physical association physical association false false false rnf2-bmi1-3 3174988 N/A 3174997 unspecified role unspecified role prey prey spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3174998 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm physical association physical association false false false rnf2-bmi1-4 3174989 N/A 3174996 unspecified role unspecified role prey prey Region 222-1012 Region 222-1012 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3174997 unspecified role unspecified role prey prey spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3174998 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm association association false false false rnf2-phc1-1 3174990 N/A 3174995 unspecified role unspecified role prey prey Region 358-664 Region 358-664 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3174996 unspecified role unspecified role prey prey Region 222-1012 Region 222-1012 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3174998 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm association association false false false bmi1-phc1-2 3174991 N/A 3174996 unspecified role unspecified role unspecified role unspecified role Region 222-1012 Region 222-1012 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3174997 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3174998 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm physical association physical association false false false phc1-rnf2-1 3174992 N/A 3174995 unspecified role unspecified role unspecified role unspecified role Region 358-664 Region 358-664 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3174996 unspecified role unspecified role unspecified role unspecified role Region 222-1012 Region 222-1012 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3174998 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm physical association physical association false false false gmnn-ub-1 3174993 N/A 3174999 unspecified role unspecified role prey prey Biotin-tag Biotin-tag biotin tag biotin tag n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false ecolx Escherichia coli N/A 3175000 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli physical association physical association false false false bmi1-rnf2-8 3174994 N/A 3174997 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3174998 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction false false false