DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ carrera-2008-1 in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Carrera I., Janody F., Leeds N., Duveau F., Treisman JE. DIP original experiment: DIP-68820X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Pygopus activates Wingless target gene transcription through the mediator complex subunits Med12 and Med13. Carrera I., Janody F., Leeds N., Duveau F., Treisman JE. Only protein-protein interactions imex curation carrera-2008-2 drome Drosophila melanogaster (Fruit fly) Fruit fly anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Carrera I., Janody F., Leeds N., Duveau F., Treisman JE. DIP original experiment: DIP-68818X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Pygopus activates Wingless target gene transcription through the mediator complex subunits Med12 and Med13. Carrera I., Janody F., Leeds N., Duveau F., Treisman JE. Only protein-protein interactions imex curation carrera-2008-3 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Carrera I., Janody F., Leeds N., Duveau F., Treisman JE. DIP original experiment: DIP-68819X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Pygopus activates Wingless target gene transcription through the mediator complex subunits Med12 and Med13. Carrera I., Janody F., Leeds N., Duveau F., Treisman JE. Only protein-protein interactions imex curation med12_drome Mediator of RNA polymerase II transcription subunit 12 kto CG8491 Trap230 Mediator complex subunit 12 Mediator complex subunit Kohtalo dTRAP230 Med12 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MLSMLQEKRPLKRTRLGPPDIYPQDAKQREDELTPTNVKHGFTTTPPLSDEFGTAHNSNVNASKVSAFFSGVLAKKEELMTLPDTGRKKQQINCKDNFWPVSPRRKCTVDAWFKDLAGNKPLLSLAKRAPSFNKKEEIFITLCENQVNMQRATWFIKLSAAYTLSFTESKNKKRSIYDPAAEWTGNMIKFMKELLPKLQEYYQQNHDKSSSNGTTSGSLTAAGNGPASNGSTGTSSINSVTGSSASTNVIPVPSMASPLPPIHSPANGQQAAPGGGVNAGSVMPTTGSLGGVVGGPGSSVVGGAAGAGAAVPPGSTISGIGSQFEDSRNALKYWKYCHQLSKYMYEESLLDRQEFLNWILDLLDKMRTQASFDEPLKKLVLSFALQYMHDFVQSERLCRKMAYIVSKKLAQLLNTVVEQQTIKELDEPKLQQDPYELALQEQMSCPHHRDIVLYLSTILQIITIECPTALVWSGIAAHRAPSSLLGSPLDHLPLAPSVLPMPTRCPRTNHEIRRQLRAAESDIVLRTQHAEQRWFAAKWLSAGKNQYTSVLATLDHLDTHCFDRMEHNNSIDTLYAQIFPSPTVSRRREEDQVEPRPPYEPKQDKDTVRILCEWAVSGQRWGEHRAMVVAILLDKRQIDVTSTPADQQSSDKDDKDSLASGAGLIDGLPVFQHVLMHFLDHDAPVLDEHVSSPQQRTEFTNLVQLFSALIRHDVFSHNAYMHTLISRGDLLLESVLVIKSGTTATKTSPPPPAPPPTTTHGFDDDGFGGGLDFKHNEFDDSNVDDDLDKLVQNIKEKGQQHEAPDSPKIGPPGDGETNPGGSISRHYVYTKHFPIPQDDPSMSSYSSESNQRYILLFGVGKERDEKKHAVKKMSKEIGKLFTKKFSIDVAAAGHVKKHSRNEFNFEATTSKCQQMAYFDQHVVTAQCAANVLEQLNGFALGNNNYLPVQEHVAFLFDLMELALNIYSLLELCDSLLKELPEVEHQLQLKKSNLVRSYTTSLALYIVSILRRYHSCLLLSPEQTLSVFEGVCRTIRHVSNPSECTSAERCIIAYLSDLHESCVLLQGKEQSTEYYQQLQCIKRFKDIFNTPEQLDLPPQGYNPLLLQELFMAPRRGGKLDPHWLGTLHESPANVYSFVSNALIAVCRETDNERLNDVALACAELTASCNVLSEEWIYALQSLCSGSKSPRYPHLGGQVDIGQLKTHNALAVFVCILVARHCFSLADFVSKFALPTLARSVSAGGAELSVDAEAGARLTCHLVLKLFKTLEIPQPGMYSVSTSPNPLHAVGNDFSIRLSCDRHLLVGAHKTIPIAAVLAVLKAILIVVDNAALKTPLASGSGTSSGGLGGAFGSGKRSGFNTPVHPGSTPKSNEQRPADLSQILGTSDLQLGSSLTSEPEALQQPSVGGMEQISLLEFAQAVLKQICAQEHVLERCLKNAEQLCDMIIDEMLTAKQAQRVLHMICYPEPEFNIISELDQRSMIVRILENLGQWTLRISWLDLQLMYRQSLSNNAELNVWLDTVARAAIDVFHMEEVVLPGAVKATHKPKPSTWLVAPLIAKLTPAVQGRILRVAGQVLESMNYFSKVSKSDCNSSGSGDEREKSNSCHSSNSYGLGGVPARNKKMPLDYQPFLGLILTCLKGQDEYKENLLVSLYAQLSQCLQSFAELDTIGGIDEPQAREEILDALQLRFSLVGGMFEAIQKNSTPTTDWAILLAQLVCQGVVDLSCNRELFTTVVDMLATLVHSTLVSDDERHYMNLMKKLKKEIGEKNNASIRVIRQLLPLYKQPTEVIACEHSGMDTKGNKICDIDKKQLRISDKQRISVWDILEGHKNPAPLSWVWFGAVKLERKPLTYEEAHRNLKYHTHSLVKPSSYYYEPLPLPPEDIEPVPEKICIKDEMKADTPSSVDQSPSAVVGGTGRGRGKGTTTRKRKPKNPKTPPVVNTQQQQPQLAQQPQQPQNVQQQQLQQQQQQQQHMQQQHMQQQQMQPNQMGQMPMNMPMNMQQFAPNPNNMMQQNAMLQQQQQQQMQQMGNNPMQQQLNVGGGNGQPNPQMNFMQQGPGGGGAGPQGMPGQQQQWHNAPQQQQPPQPYHNQYAPHQQNMQSNRIERPPLNANSKQALSQMLRQRQPFQQQAQQGPGGGFNPMQQQPQASQQQPGPQQQMNPNQMRQQQMNPQQNPQSVAAFNAMQQQPQQNAQQQQMNPNQQQQQQFMRGGNMRPGMAPNQMNQMNMGGQGMSQNPMMQQQIPQNMVGMVNPNANQMMQSGGAQGGNGVGVGVGVGVGGAGNNPNMGMGGMPQQGMIQQQPQQQPQQQVQFQNFQNQYQQQQQQGMQQQGGGAGVGVGVGMAPNQQQQQQANMMGNFNPQMQQGNRNNPDFMAAAAVAQQQQQQQQQQRVVPGGMMAGNRNQYMNQAPNVTMSTMMGPGPGGVVGQVPPYARQQSAGGGKPGVLNTQQQFQQQQQQQQQLRHQMMQLQGMGGGAGGGMGAGPQQGGGAVGGGAGGGMVPQQQSMNQQQTPNLVAQLQRQNMMGQQQYQPPPY pygo_drome Protein pygopus pygo gam CG11518 Protein gammy legs protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MTHNLGMAPYRLPGPAGGLCPPDFKPPPPTDIISAPSNPKKRRKTSSAANSAAAVAAAAAAAAAANSMQQQQAPPTPQDLLPPPPMGGFGDTIIASNPFDDSPQVSAMSSSAAAAMAAMNQMGGGPGGGHFGGGGPGGHPHWEDRMGMGGGPPPPPHMHPHMHPHHPGGPMGHPHGPHPHMGGPPPMRGMSPMHPHQMGPGPGVGLPPHMNHGRPGGPGGPGGPVPMGSPMGGIAGMGGMSPMGGMGGPSISPHHMGMGGLSPMGGGPNGPNPRAMQGSPMGGPGQNSPMNSLPMGSPMGNPIGSPLGPPSGPGPGNPGNTGGPQQQQQQPPQPPMNNGQMGPPPLHSPLGNGPTGHGSHMPGGPIPGPGPGPGGLVGPGGISPAHGNNPGGSGNNMLGGNPGGGNSNNNGSNTSNASNNNQNPHLSPAAGRLGVPTSMQSNGPSVSSVASSSVPSPATPTLTPTSTATSMSTSVPTSSPAPPAMSPHHSLNSAGPSPGMPNSGPSPLQSPAGPNGPNNNNSNNNNGPMMGQMIPNAVPMQHQQHMGGGPPGHGPGPMPGMGMNQMLPPQQPSHLGPPHPNMMNHPHHPHHHPGGPPPHMMGGPGMHGGPAGMPPHMGGGPNPHMMGGPHGNAGPHMGHGHMGGVPGPGPGPGGMNGPPHPHMSPHHGHPHHHHNPMGGPGPNMFGGGGGGPMGPGGPMGNMGPMGGGPMGGPMGVGPKPMTMGGGKMYPPGQPMVFNPQNPNAPPIYPCGMCHKEVNDNDEAVFCESGCNFFFHRTCVGLTEAAFQMLNKEVFAEWCCDKCVSSKHIPMVKFKC med13_drome Mediator of RNA polymerase II transcription subunit 13 skd bli pap Med13 Mediator complex subunit 13 Mediator complex subunit Skuld Protein blind spot Protein poils aux pattes Trap240 CG9936 dTRAP240 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MTHQNHQTNGASLEDCHTNFYALTDLCGIKWRKFVNGERPNASSDPLADPILRSYSRCIQADMLCVWRRVQSTKTDHADPNALTFEMTTSTKVHPPLSLAAAKELWIFWYGEEPDLSELVDAELLRVAANQALWNGTWKGALTYECRSLLFKALHNLMERFVLTKDIVRFGKWFVQPCTSSDRLFGRSSQHLSFSFTFFVHGDTVCASIDLREHPAVRPLTKEHLTEAAAAFAAASSPPGSNGSAASAGGAVPNPGQDPNGASMDGLDGGEGAAKAAPPPHARKVMLAPFGIAGILTGNSYKASDPIAEKILEDWASFFPLCNKDNTDVPPVVEVVSGGHKMYHPTNYVLVTDLDDMEHMEFVEMQKMQSSVAGAAAESAVLASLSCPPGAASAPSSMGAAPSATAVPLGPASLNAPALAGAGVGATASSAAKEISRKAAPQAVSALERLAFQPYYDQRPTSGFTFNTNNTHIPASAAVEMPERTWQDCVMNTLHVDAAAAAAAVASSTPASGTGSLSADGDENEQNKPPQDSKQLVQQQIQQQQQRQKLWNFVDPMQKAPCICTKHLGNTPHGTPHGGASTYSRNSLGGDSSMPVASVESPATPAPSPHPNSAHSQPTSVPPAEQLLNMSPHAPTSVSNLQQPPTPIDHLLDKNTPAPTPTDQHDSKSITASPYVHQTPSVEPPSYTDHAAGGGPAGGQGLGTGPGSVPAQQPATPTAATSAGGAGSGGPSNAIGANAVGTISVKKLEMQQQTPSAAMAIKQEPGAQGRGVGGVTSTTEALNNFKRLYNPPKLTLKDPDSFYDEEWLKEVIYDFQYQEYWDYSTVKRPKMEKQRRPRYAKNLYEGQNHVKPVMPSPGSVYGSQLLSLDESASQAGGRGGGGQAAGSSGGGLVGIANSTASGSDVEADGSSFFQGLNIKTEPGLHSPSCKETSKSSGGNSSGGGSGSGGNLFTAEGLNPSLNDLEQLFETSSNDECSSVQIHTPPDSNNPSNGGCSAVTNTIEDLKRSTAVASAAVAAAAAAAASGAGNIQAEDLTKMFPTPPSHEQQHPNSSPCQTDVVMTDLSVDTTTTIITSSITTTCNTTITSSINTTTTACSNPSNSIMLAAAQAPVTVVAIQTVSKMVKQEYNLELGSPMEEPINDWDYVYRPPQQEKFVGSTRYAPLTNLPSQTQPPLTLPTGCFYQPTWSSHKSRAATLAKAAAAQQQQHQKHQALQQRIQLHQQKLQQLQLQNQQQQQAAAAAAAAASGGVGHQKHQHQHLHDLLSAAPRTPLTPSTVPQPLSSGGSQYLLNQLNCPQAPPGASMQQLMHRAGMSPISPGPGMGPYAARSSPMSRATPTHPPPPYPYDLAVASPATSTSSYLNRPLHSQEHPHMHGLGGGATGVVGHGTGGGGHMGMVAYTGDAGIVSGGTAMAAGSSSLLQELPEVNSVLVNILLYDTALNVFRDHNFDSSSVCVCNADTQKIGNIRGADSGVYVPLPGVSFNPFPSGAGGALAGQRMLNGPSSAGFGGMRMISAFGGSPASASMPGAGSGHGHGPNGGSNSSSCTPPSSNPHITGYVDDDPVECTCGFSAVVNRRLSHRAGLFYEDEVEITGIADDPGRNKQPTLLSIIQSLSRKNQNKQGPGETSSALDKIGAGGLPNGQLEQLGHAVFDLLLDQCSIIQTSSSSVHRALQSHRRRMSRQRRIFGNNGAPTASLASIANVLEFMDAHDVISLALEQSRLAFENQRMDNMMDFHGNGSSSSHQQQQLTAFHAPPPALRHKLAGIGAGRLTVHKWPYLPVGFTRSNKEIVRTMNAIQPMLQNAFHCKSRGGSGSKDASSYNTVSGPLTWRQFHRLAGRASGQCEPQPIPSVVVGYEKDWISVAPHSIHYWDKFLLEPYSYARDVVYVVVCPDNEHVVNCTRSYFRELSSTYEMCKLGKHTPIRGWDGFLQVGAARNNVPADRETTPLDDWLRTLEHAALAEQIRRYAVAFIHQLAPYLSRVPNDKTLLNPPDGSGNSHSKGGSSCSSNSSSVSGLPGGDLPTDNIKLEPGTEPQVQPMETNEIKQEPGVGKGGTAAGETKPTLILGDPLGMGETLEDINPSAIVLYVVNPFTFASDSCELERLALIALLRCYAELLKAVPDSVRSQMNIQIISLESVMELGPCGNRKRFSDEIRCLALNIFSQCRRHLVHAQSVKSLTGFGTAANMEAFLKTKDEPNRRAYKMYTAPFVLAPMHERNDKTDFSRSAGSMHGQNEHRYSVMYCNYCLSEDQAWLLATATDERGEMLEKICINIDVPNRARRRKAPARYVALKKLMDFIMGIISQTSQMWRLVIGRIGRIGHSELKSWSFLLSKQQLQKASKQFKDMCKQCTLMYPPTILSACLVTLEPDAKLRVMPDQFTPDERFSQISMQNPLATPQDVTCTHILVFPTSAVCAPFTRQFQNEPQVDDDFLTFEEEGNEDFSDADIGDLFWDTHMDRVSNHGSPGRMDDNRSWQSAGGNNFKCTPPQEVEEVGSLNQQPISVGYMVSTAPTGRMPAWFWSACPHLEDVCPVFLKTALHLHVPSIQSADDILNSTNAHQSGNDHPLDSNLTADVLRFVLEGYNALSWLALDSNTHDRLSCLPINVQTLMDLYYLTAAIA pygo-kto-1 3170991 N/A 3170994 unspecified role unspecified role prey prey Region 1-408 Region 1-408 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Region 628-2531 Region 628-2531 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3170995 unspecified role unspecified role bait bait Region 1-181 Region 1-181 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false skd-pygo-1 3170992 N/A 3170995 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3170996 unspecified role unspecified role bait bait drome Drosophila melanogaster (Fruit fly) Fruit fly physical association physical association false false false pygo-skd-1 3170993 N/A 3170995 unspecified role unspecified role bait bait Region 1-181 Region 1-181 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3170996 unspecified role unspecified role prey prey Region 1-350 Region 1-350 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false