DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ ziegenfuss-2008-1 drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Ziegenfuss JS., Biswas R., Avery MA., Hong K., Sheehan AE., Yeung YG., Stanley ER., Freeman MR. DIP original experiment: DIP-250373X Nature 2008 IMEx Draper-dependent glial phagocytic activity is mediated by Src and Syk family kinase signalling. Ziegenfuss JS., Biswas R., Avery MA., Hong K., Sheehan AE., Yeung YG., Stanley ER., Freeman MR. Only protein-protein interactions imex curation ziegenfuss-2008-2 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Ziegenfuss JS., Biswas R., Avery MA., Hong K., Sheehan AE., Yeung YG., Stanley ER., Freeman MR. DIP original experiment: DIP-250371X Nature 2008 IMEx Draper-dependent glial phagocytic activity is mediated by Src and Syk family kinase signalling. Ziegenfuss JS., Biswas R., Avery MA., Hong K., Sheehan AE., Yeung YG., Stanley ER., Freeman MR. Only protein-protein interactions imex curation ziegenfuss-2008-3 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Ziegenfuss JS., Biswas R., Avery MA., Hong K., Sheehan AE., Yeung YG., Stanley ER., Freeman MR. DIP original experiment: DIP-250372X Nature 2008 IMEx Draper-dependent glial phagocytic activity is mediated by Src and Syk family kinase signalling. Ziegenfuss JS., Biswas R., Avery MA., Hong K., Sheehan AE., Yeung YG., Stanley ER., Freeman MR. Only protein-protein interactions imex curation shark_drome Tyrosine-protein kinase Shark Shark Tk7 CG18247 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSRDSDPMKWYHGNLSREAADELLKQGYEDGTFLVRESSTAAGDFVLSLLCQGEVCHYQVRRHGGEDAFFSIDDKVQTKILHGLDTLVDYYQQAANGLPTKLTVPLIRDLPPHNTRSHGVTNLLHRATSKNESKVVFELLKCGYRNFDAKNQDGQTALHLAALHSDEDILKHLLNAKVQVNSSDSFGCQPLHYAARSKPASFIRTLISAQANVQGRNIDNGYVPLHEAAKHGNLEAVQELLLAEAPPLPRTSSGEFPFDLAKEAGQTAVEEFLLNYKLPPANTTRDQWYHGTLTREEAVAILKKHAKELLAKQPEVDTSGCFLVRYSESPAASGLVLTLLCDQVVKNFRISQADLYQNGNKVQSGGSKFLYIDDGPYWPSVEHLIAHFMRFSYGLPVSLKYPVPPQPKPEVPSFATIPRSNMKPKAASPATPPTPVSPHSHHQHPHVPALTITKKKQKENSSSMFNTLRLTSPKKALFDMNSLRKNKSKGKRSDSESSVSGSLAGTEQELQAAAPMLKSLSFSTEFSTFNADGVTGSGAAAAGEVYNVPRNNTPIEIDLPPIAQKTEAEVEYFTKSDVAIERERAGQWIGNGYQPTMDVLSLLDQQIKAPAVARLNSLGPNASTESEMASYLHRKCSGTPSTPSATEVEAAKLRFFIEPEKLVLDREIGHGEFGSVHSGWLLRKSGAGEESRLEVAIKMLSDEHSNKQEFLREASVMMRLEHKCIVRLIGIAKGEMLMMVQELAPLGSMLQYILDHGHEITANAELKVWASQIACGMHYLESQHFVHRDLAARNILLTARHQAKISDFGMSRSLRPGSTEYQFTQGGRWPIRWYAPESFNLGIFSHASDVWSFGVTIWEMFSLGAPPYGEISNVDAIKLVDSGERLPQPNLCPAYIYAVMQSCWKERPKDRPTFVYLTEFFARDPDYQNLPELVQTVHI drpr_drome Protein draper drpr Megf10 CG2086 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MLPVILIACLAQLVLAQADLKDLDGPNICKRRELYNVDVVYTELQSFQERGSTWCVTFPPRCSTYRIKHRVVNKTKTIAKNRIVRDCCDGYIASAGECVPHCSEPCQHGRCISPEKCKCDHGYGGPACDINCPPGWYGRNCSMQCDCLNNAVCEPFSGDCECAKGYTGARCADICPEGFFGANCSEKCRCENGGKCHHVSGECQCAPGFTGPLCDMRCPDGKHGAQCQQDCPCQNDGKCQPETGACMCNPGWTGDVCANKCPVGSYGPGCQESCECYKGAPCHHITGQCECPPGYRGERCFDECQLNTYGFNCSMTCDCANDAMCDRANGTCICNPGWTGAKCAERICEANKYGLDCNRTCECDMEHTDLCHPETGNCQCSIGWSSAQCTRPCTFLRYGPNCELTCNCKNGAKCSPVNGTCLCAPGWRGPTCEESCEPGTFGQDCALRCDCQNGAKCEPETGQCLCTAGWKNIKCDRPCDLNHFGQDCAKVCDCHNNAACNPQNGSCTCAAGWTGERCERKCDTGKFGHDCAQKCQCDFNNSLACDATNGRCVCKQDWGGVHCETNCRSGYYGENCDKVCRCLNNSSCDPDSGNCICSAGWTGADCAEPCPPGFYGMECKERCPEILHGNKSCDHITGEILCRTGYIGLTCEHPCPAGLYGPGCKLKCNCEHGGECNHVTGQCQCLPGWTGSNCNESCPTDTYGQGCAQRCRCVHHKVCRKADGMCICETGWSGTRCDEVCPEGFYGEHCMNTCACPSANFQCHAAHGCVCRSGYTGDNCDELIASQRIADQSENSSRASVALTLVLMTLFACIIFAVFIYYRRRVSNLKTEIAHVHYTHDTNPPSWPPNHNFDNPVYGMQAETRLLPNNMRSKMNNFDQRSTMSTDYGDDCNASGRVGSYSINYNHDLLTKNLNADRTNPIVYNESLKEEHVYDEIKHKEGYKDPDEYDHLDYSRPSTSQKPHYHRMNDAMLNINQDEEKPSNVKNMTVLLNKPLPPTEPEPQHECFDNTNTNLDNVSTASPSSSPKFLK drpr-shark-1 3170684 N/A 3170687 unspecified role unspecified role prey prey Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3170688 unspecified role unspecified role bait bait Q9W0A0:p.Tyr949Phe Y949F mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false drome Drosophila melanogaster (Fruit fly) Fruit fly physical association physical association false false false shark-drpr-1 3170685 N/A 3170687 unspecified role unspecified role bait bait Region 10-468 Region 10-468 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3170688 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false shark-drpr-2 3170686 N/A 3170687 unspecified role unspecified role bait bait Region 10-468 Region 10-468 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3170688 unspecified role unspecified role prey prey Q9W0A0:p.Tyr949Phe Y949F mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q9W0A0:p.Tyr934Phe T934F mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false