DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ westbrook-2008-1 human Homo sapiens Human pull down pull down anti tag western anti tag western blot anti tag western Westbrook TF., Hu G., Ang XL., Mulligan P., Pavlova NN., Liang A., Leng Y., Maehr R., Shi Y., Harper JW., Elledge SJ. DIP original experiment: DIP-259547X Nature 2008 IMEx SCFbeta-TRCP controls oncogenic transformation and neural differentiation through REST degradation. Westbrook TF., Hu G., Ang XL., Mulligan P., Pavlova NN., Liang A., Leng Y., Maehr R., Shi Y., Harper JW., Elledge SJ. Only protein-protein interactions imex curation westbrook-2008-2 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Westbrook TF., Hu G., Ang XL., Mulligan P., Pavlova NN., Liang A., Leng Y., Maehr R., Shi Y., Harper JW., Elledge SJ. DIP original experiment: DIP-259541X Nature 2008 IMEx SCFbeta-TRCP controls oncogenic transformation and neural differentiation through REST degradation. Westbrook TF., Hu G., Ang XL., Mulligan P., Pavlova NN., Liang A., Leng Y., Maehr R., Shi Y., Harper JW., Elledge SJ. Only protein-protein interactions imex curation westbrook-2008-3 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Westbrook TF., Hu G., Ang XL., Mulligan P., Pavlova NN., Liang A., Leng Y., Maehr R., Shi Y., Harper JW., Elledge SJ. DIP original experiment: DIP-259544X Nature 2008 IMEx SCFbeta-TRCP controls oncogenic transformation and neural differentiation through REST degradation. Westbrook TF., Hu G., Ang XL., Mulligan P., Pavlova NN., Liang A., Leng Y., Maehr R., Shi Y., Harper JW., Elledge SJ. Only protein-protein interactions imex curation westbrook-2008-4 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Westbrook TF., Hu G., Ang XL., Mulligan P., Pavlova NN., Liang A., Leng Y., Maehr R., Shi Y., Harper JW., Elledge SJ. DIP original experiment: DIP-259542X Nature 2008 IMEx SCFbeta-TRCP controls oncogenic transformation and neural differentiation through REST degradation. Westbrook TF., Hu G., Ang XL., Mulligan P., Pavlova NN., Liang A., Leng Y., Maehr R., Shi Y., Harper JW., Elledge SJ. Only protein-protein interactions imex curation westbrook-2008-5 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Westbrook TF., Hu G., Ang XL., Mulligan P., Pavlova NN., Liang A., Leng Y., Maehr R., Shi Y., Harper JW., Elledge SJ. DIP original experiment: DIP-259546X Nature 2008 IMEx SCFbeta-TRCP controls oncogenic transformation and neural differentiation through REST degradation. Westbrook TF., Hu G., Ang XL., Mulligan P., Pavlova NN., Liang A., Leng Y., Maehr R., Shi Y., Harper JW., Elledge SJ. Only protein-protein interactions imex curation westbrook-2008-6 in vitro In vitro pull down pull down autoradiography autoradiography Westbrook TF., Hu G., Ang XL., Mulligan P., Pavlova NN., Liang A., Leng Y., Maehr R., Shi Y., Harper JW., Elledge SJ. DIP original experiment: DIP-259548X Nature 2008 IMEx SCFbeta-TRCP controls oncogenic transformation and neural differentiation through REST degradation. Westbrook TF., Hu G., Ang XL., Mulligan P., Pavlova NN., Liang A., Leng Y., Maehr R., Shi Y., Harper JW., Elledge SJ. Only protein-protein interactions imex curation westbrook-2008-7 human Homo sapiens Human pull down pull down anti tag western anti tag western blot anti tag western Westbrook TF., Hu G., Ang XL., Mulligan P., Pavlova NN., Liang A., Leng Y., Maehr R., Shi Y., Harper JW., Elledge SJ. DIP original experiment: DIP-259545X Nature 2008 IMEx SCFbeta-TRCP controls oncogenic transformation and neural differentiation through REST degradation. Westbrook TF., Hu G., Ang XL., Mulligan P., Pavlova NN., Liang A., Leng Y., Maehr R., Shi Y., Harper JW., Elledge SJ. Only protein-protein interactions imex curation westbrook-2008-8 spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Westbrook TF., Hu G., Ang XL., Mulligan P., Pavlova NN., Liang A., Leng Y., Maehr R., Shi Y., Harper JW., Elledge SJ. DIP original experiment: DIP-259543X Nature 2008 IMEx SCFbeta-TRCP controls oncogenic transformation and neural differentiation through REST degradation. Westbrook TF., Hu G., Ang XL., Mulligan P., Pavlova NN., Liang A., Leng Y., Maehr R., Shi Y., Harper JW., Elledge SJ. Only protein-protein interactions imex curation fbw1b_human F-box/WD repeat-containing protein 11 KIAA0696 FBXW11 BTRCP2 FBW1B FBXW1B F-box/WD repeat-containing protein 1B F-box and WD repeats protein beta-TrCP2 Homologous to Slimb protein protein protein human Homo sapiens Human MEPDSVIEDKTIELMCSVPRSLWLGCANLVESMCALSCLQSMPSVRCLQISNGTSSVIVSRKRPSEGNYQKEKDLCIKYFDQWSESDQVEFVEHLISRMCHYQHGHINSYLKPMLQRDFITALPEQGLDHIAENILSYLDARSLCAAELVCKEWQRVISEGMLWKKLIERMVRTDPLWKGLSERRGWDQYLFKNRPTDGPPNSFYRSLYPKIIQDIETIESNWRCGRHNLQRIQCRSENSKGVYCLQYDDEKIISGLRDNSIKIWDKTSLECLKVLTGHTGSVLCLQYDERVIVTGSSDSTVRVWDVNTGEVLNTLIHHNEAVLHLRFSNGLMVTCSKDRSIAVWDMASATDITLRRVLVGHRAAVNVVDFDDKYIVSASGDRTIKVWSTSTCEFVRTLNGHKRGIACLQYRDRLVVSGSSDNTIRLWDIECGACLRVLEGHEELVRCIRFDNKRIVSGAYDGKIKVWDLQAALDPRAPASTLCLRTLVEHSGRVFRLQFDEFQIISSSHDDTILIWDFLNVPPSAQNETRSPSRTYTYISR rest_human RE1-silencing transcription factor REST Neural-restrictive silencer factor X2 box repressor NRSF XBR protein protein human Homo sapiens Human MATQVMGQSSGGGGLFTSSGNIGMALPNDMYDLHDLSKAELAAPQLIMLANVALTGEVNGSCCDYLVGEERQMAELMPVGDNNFSDSEEGEGLEESADIKGEPHGLENMELRSLELSVVEPQPVFEASGAPDIYSSNKDLPPETPGAEDKGKSSKTKPFRCKPCQYEAESEEQFVHHIRVHSAKKFFVEESAEKQAKARESGSSTAEEGDFSKGPIRCDRCGYNTNRYDHYTAHLKHHTRAGDNERVYKCIICTYTTVSEYHWRKHLRNHFPRKVYTCGKCNYFSDRKNNYVQHVRTHTGERPYKCELCPYSSSQKTHLTRHMRTHSGEKPFKCDQCSYVASNQHEVTRHARQVHNGPKPLNCPHCDYKTADRSNFKKHVELHVNPRQFNCPVCDYAASKKCNLQYHFKSKHPTCPNKTMDVSKVKLKKTKKREADLPDNITNEKTEIEQTKIKGDVAGKKNEKSVKAEKRDVSKEKKPSNNVSVIQVTTRTRKSVTEVKEMDVHTGSNSEKFSKTKKSKRKLEVDSHSLHGPVNDEESSTKKKKKVESKSKNNSQEVPKGDSKVEENKKQNTCMKKSTKKKTLKNKSSKKSSKPPQKEPVEKGSAQMDPPQMGPAPTEAVQKGPVQVEPPPPMEHAQMEGAQIRPAPDEPVQMEVVQEGPAQKELLPPVEPAQMVGAQIVLAHMELPPPMETAQTEVAQMGPAPMEPAQMEVAQVESAPMQVVQKEPVQMELSPPMEVVQKEPVQIELSPPMEVVQKEPVKIELSPPIEVVQKEPVQMELSPPMGVVQKEPAQREPPPPREPPLHMEPISKKPPLRKDKKEKSNMQSERARKEQVLIEVGLVPVKDSWLLKESVSTEDLSPPSPPLPKENLREEASGDQKLLNTGEGNKEAPLQKVGAEEADESLPGLAANINESTHISSSGQNLNTPEGETLNGKHQTDSIVCEMKMDTDQNTRENLTGINSTVEEPVSPMLPPSAVEEREAVSKTALASPPATMAANESQEIDEDEGIHSHEGSDLSDNMSEGSDDSGLHGARPVPQESSRKNAKEALAVKAAKGDFVCIFCDRSFRKGKDYSKHLNRHLVNVYYLEEAAQGQE fbw1a_human F-box/WD repeat-containing protein 1A BTRC BTRCP FBW1A FBXW1A F-box and WD repeats protein beta-TrCP E3RSIkappaB pIkappaBalpha-E3 receptor subunit Epididymis tissue protein Li 2a protein protein human Homo sapiens Human MDPAEAVLQEKALKFMCSMPRSLWLGCSSLADSMPSLRCLYNPGTGALTAFQNSSEREDCNNGEPPRKIIPEKNSLRQTYNSCARLCLNQETVCLASTAMKTENCVAKTKLANGTSSMIVPKQRKLSASYEKEKELCVKYFEQWSESDQVEFVEHLISQMCHYQHGHINSYLKPMLQRDFITALPARGLDHIAENILSYLDAKSLCAAELVCKEWYRVTSDGMLWKKLIERMVRTDSLWRGLAERRGWGQYLFKNKPPDGNAPPNSFYRALYPKIIQDIETIESNWRCGRHSLQRIHCRSETSKGVYCLQYDDQKIVSGLRDNTIKIWDKNTLECKRILTGHTGSVLCLQYDERVIITGSSDSTVRVWDVNTGEMLNTLIHHCEAVLHLRFNNGMMVTCSKDRSIAVWDMASPTDITLRRVLVGHRAAVNVVDFDDKYIVSASGDRTIKVWNTSTCEFVRTLNGHKRGIACLQYRDRLVVSGSSDNTIRLWDIECGACLRVLEGHEELVRCIRFDNKRIVSGAYDGKIKVWDLVAALDPRAPAGTLCLRTLVEHSGRVFRLQFDEFQIVSSSHDDTILIWDFLNDPAAQAEPPRSPSRTYTYISR ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG fbxw11-rest-1 3169751 N/A 3169759 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3169760 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false rest-btrc-4 3169752 N/A 3169761 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3169760 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association false false false ub-rest-1 3169753 N/A 3169762 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false human Homo sapiens Human N/A 3169760 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false rest-btrc-5 3169754 N/A 3169761 unspecified role unspecified role prey prey GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3169760 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false btrc-rest-1 3169755 N/A 3169761 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3169760 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false rest-btrc-6 3169756 N/A 3169761 unspecified role unspecified role prey prey 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro N/A 3169760 unspecified role unspecified role bait bait Region 1021-1035 Region 1021-1035 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Biotin-tag Biotin-tag biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false in vitro In vitro direct interaction direct interaction false false false btrc-rest-2 3169757 N/A 3169761 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3169760 unspecified role unspecified role prey prey Q13127:p.Ser1024Ala S1024A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q13127:p.Ser1027Ala S1027A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q13127:p.Ser1030Ala S1030A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false btrc-rest-3 3169758 N/A 3169761 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3169760 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm physical association physical association false false false