DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ harada-2008-1 ecolx Escherichia coli 3d-em 3D electron microscopy 3D-EM electron tomog predetermined predetermined participant predetermined Harada BT., Knight MJ., Imai S., Qiao F., Ramachander R., Sawaya MR., Gingery M., Sakane F., Bowie JU. DIP original experiment: DIP-68166X Structure (London, England : 1993) 2008 IMEx Regulation of enzyme localization by polymerization: polymer formation by the SAM domain of diacylglycerol kinase delta1. Harada BT., Knight MJ., Imai S., Qiao F., Ramachander R., Sawaya MR., Gingery M., Sakane F., Bowie JU. Only protein-protein interactions imex curation harada-2008-2 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Harada BT., Knight MJ., Imai S., Qiao F., Ramachander R., Sawaya MR., Gingery M., Sakane F., Bowie JU. DIP original experiment: DIP-68164X Structure (London, England : 1993) 2008 IMEx Regulation of enzyme localization by polymerization: polymer formation by the SAM domain of diacylglycerol kinase delta1. Harada BT., Knight MJ., Imai S., Qiao F., Ramachander R., Sawaya MR., Gingery M., Sakane F., Bowie JU. Only protein-protein interactions imex curation harada-2008-3 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Harada BT., Knight MJ., Imai S., Qiao F., Ramachander R., Sawaya MR., Gingery M., Sakane F., Bowie JU. DIP original experiment: DIP-68167X Structure (London, England : 1993) 2008 IMEx Regulation of enzyme localization by polymerization: polymer formation by the SAM domain of diacylglycerol kinase delta1. Harada BT., Knight MJ., Imai S., Qiao F., Ramachander R., Sawaya MR., Gingery M., Sakane F., Bowie JU. Only protein-protein interactions imex curation harada-2008-4 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Harada BT., Knight MJ., Imai S., Qiao F., Ramachander R., Sawaya MR., Gingery M., Sakane F., Bowie JU. DIP original experiment: DIP-68168X Structure (London, England : 1993) 2008 IMEx Regulation of enzyme localization by polymerization: polymer formation by the SAM domain of diacylglycerol kinase delta1. Harada BT., Knight MJ., Imai S., Qiao F., Ramachander R., Sawaya MR., Gingery M., Sakane F., Bowie JU. Only protein-protein interactions imex curation harada-2008-5 ecolx Escherichia coli solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Harada BT., Knight MJ., Imai S., Qiao F., Ramachander R., Sawaya MR., Gingery M., Sakane F., Bowie JU. DIP original experiment: DIP-68165X Structure (London, England : 1993) 2008 IMEx Regulation of enzyme localization by polymerization: polymer formation by the SAM domain of diacylglycerol kinase delta1. Harada BT., Knight MJ., Imai S., Qiao F., Ramachander R., Sawaya MR., Gingery M., Sakane F., Bowie JU. Only protein-protein interactions imex curation q16760-2 Diacylglycerol kinase delta DGKD 130 kDa diacylglycerol kinase Diglyceride kinase delta KIAA0145 DGKdelta1 protein protein human Homo sapiens Human MNMFLYFQTIIKEGMLTKQNNSFQRSKRRYFKLRGRTLYYAKTAKSIIFDEVDLTDASVAESSTKNVNNSFTVITPCRKLILCADNRKEMEDWIAALKTVQNREHFEPTQYSMDHFSGMHNWYACSHARPTYCNVCREALSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASIGKDIIEDADGIAMPHQWLEGNLPVSAKCTVCDKTCGSVLRLQDWRCLWCKAMVHTSCKESLLTKCPLGLCKVSVIPPTALNSIDSDGFWKASCPPSCTSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDTFRILVCGGDGSVGWVLSEIDSLNLHKQCQLGVLPLGTGNDLARVLGWGSACDDDTQLPQILEKLERASTKMLDRWSVMAYEAKLPRQASSSTVTEDFSEDSEVQQILFYEDSVAAHLSKILTSDQHSVVISSAKVLCETVKDFVARVGKAYEKTTESSEESEVMAKKCSVLKEKLDSLLKTLDDESQASSSLPNPPPTIAEEAEDGDGSGSICGSTGDRLVASACPARPQIFRPREQLMLRANSLKKAIRQIIEHTEKAVDEQNAQTQEQEGFVLGLSESEEKMDHRVCPPLSHSESFGVPKGRSQRKVSKSPCEKLISKGSLSLGSSASLPPQPGSRDGLPALNTKILYPNVRAGMSGSLPGGSVISRLLINADPFNSEPETLEYYTEKCVMNNYFGIGLDAKISLDFNNKRDEHPEKCRSRTKNMMWYGVLGTKELLHRTYKNLEQKVLLECDGRPIPLPSLQGIAVLNIPSYAGGTNFWGGTKEDDTFAAPSFDDKILEVVAVFGSMQMAVSRVIRLQHHRIAQCRTVKISILGDEGVPVQVDGEAWVQPPGYIRIVHKNRAQTLTRDRAFESTLKSWEDKQKCELPRPPSCSLHPEMLSEEEATQMDQFGQAAGVLIHSIREIAQSHRDMEQELAHAVNASSKSMDRVYGKPRTTEGLNCSFVLEMVNNFRALRSETELLLSGKMALQLDPPQKEQLGSALAEMDRQLRRLADTPWLCQSAEPGDEESVMLDLAKRSRSGKFRLVTKFKKEKNNKNKEAHSSLGAPVHLWGTEEVAAWLEHLSLCEYKDIFTRHDIRGSELLHLERRDLKDLGVTKVGHMKRILCGIKELSRSAPAVEA dgkd-1 3169300 N/A 3169305 unspecified role unspecified role unspecified role unspecified role Region 1097-1164 Region 1097-1164 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false dgkd-2 3169301 N/A 3169305 unspecified role unspecified role unspecified role unspecified role Region 1097-1164 Region 1097-1164 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Q16760-2:p.Val1148Glu V1148E mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q16760-2:p.Gly1149Asp G1149D mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q16760-2:p.Lys1152Glu K1152E mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q16760-2:p.Glu1131Gly E1131G mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q16760-2:p.Asp1139Gly D1139G mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false dgkd-3 3169302 N/A 3169305 unspecified role unspecified role unspecified role unspecified role Region 1097-1164 Region 1097-1164 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false dgkd-dgkd-1 3169303 N/A 3169305 unspecified role unspecified role prey prey Region 1097-1164 Region 1097-1164 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3169305 unspecified role unspecified role bait bait Region 1097-1164 Region 1097-1164 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false dgkd-4 3169304 N/A 3169305 unspecified role unspecified role unspecified role unspecified role Region 1097-1164 Region 1097-1164 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Q16760-2:p.Val1148Glu V1148E mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false