DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ van-2008-1 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Van Petegem F., Duderstadt KE., Clark KA., Wang M., Minor DL. DIP original experiment: DIP-68389X Structure (London, England : 1993) 2008 IMEx Alanine-scanning mutagenesis defines a conserved energetic hotspot in the CaValpha1 AID-CaVbeta interaction site that is critical for channel modulation. Van Petegem F., Duderstadt KE., Clark KA., Wang M., Minor DL. Only protein-protein interactions imex curation van-2008-2 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Van Petegem F., Duderstadt KE., Clark KA., Wang M., Minor DL. DIP original experiment: DIP-68391X Structure (London, England : 1993) 2008 IMEx Alanine-scanning mutagenesis defines a conserved energetic hotspot in the CaValpha1 AID-CaVbeta interaction site that is critical for channel modulation. Van Petegem F., Duderstadt KE., Clark KA., Wang M., Minor DL. Only protein-protein interactions imex curation van-2008-3 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Van Petegem F., Duderstadt KE., Clark KA., Wang M., Minor DL. DIP original experiment: DIP-68390X Structure (London, England : 1993) 2008 IMEx Alanine-scanning mutagenesis defines a conserved energetic hotspot in the CaValpha1 AID-CaVbeta interaction site that is critical for channel modulation. Van Petegem F., Duderstadt KE., Clark KA., Wang M., Minor DL. Only protein-protein interactions imex curation q8vgc3-2 Voltage-dependent L-type calcium channel subunit beta-2 Cacnb2 Cacnlb2 Calcium channel voltage-dependent subunit beta 2 protein protein rat Rattus norvegicus (Rat) Rat MQCCGLVHRRRVRVSRGSADSYTSRPSDSDVSLEEDREAVRREAERQAQAQLEKAKTKPVAFAVRTNVRYSAAQEDDVPVPGMAISFEAKDFLHVKEKFNNDWWIGRLVKEGCEIGFIPSPVKLENMRLQHEQRAKQGKFYSSKSGGNSSSSLGDIVPSSRKSTPPSSAIDIDATGLDAEENDIPANHRSPKPSANSVTSPHSKEKRMPFFKKTEHTPPYDVVPSMRPVVLVGPSLKGYEVTDMMQKALFDFLKHRFEGRISITRVTADISLAKRSVLNNPSKHAIIERSNTRSSLAEVQSEIERIFELARTLQLVVLDADTINHPAQLSKTSLAPIIVYVKISSPKVLQRLIKSRGKSQAKHLNVQMVAADKLAQCPPQESFDVILDENQLEDACEHLADYLEAYWKATHPPSSNLPNPLLSRTLATSTLPLSPTLASNSQGSQGDQRTDRSAPRSASQAEEEPCLEPVKKSQHRSSSATHQNHRSGTGRGLSRQETFDSETQESRDSAYVEPKEDYSHEHVDRYVPHREHNHREESHSSNGHRHREPRHRTRDMGRDQDHNECSKQRSRHKSKDRYCDKEGEVISKRRSEAGEWNRDVYIRQ cac1c_human Voltage-dependent L-type calcium channel subunit alpha-1C CACNA1C CACH2 CACN2 CACNL1A1 CCHL1A1 Voltage-gated calcium channel subunit alpha Cav1.2 Calcium channel, L type, alpha-1 polypeptide, isoform 1, cardiac muscle protein protein human Homo sapiens Human MVNENTRMYIPEENHQGSNYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGMDEEKPRNMSMPTSETESVNTENVAGGDIEGENCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSNVFYWLVIFLVFLNTLTIASEHYNQPNWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFVVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQELVEKPAVGESKEEKIELKSITADGESPPATKINMDDLQPNENEDKSPYPNPETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSSNNRFRLQCHRIVNDTIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKILGNADYVFTSIFTLEIILKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMILKLIAFKPKGYFSDPWNVFDFLIVIGSIIDVILSETNHYFCDAWNTFDALIVVGSIVDIAITEVNPAEHTQCSPSMNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPSNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQAGLRTLHDIGPEIRRAISGDLTAEEELDKAMKEAVSAASEDDIFRRAGGLFGNHVSYYQSDGRSAFPQTFTTQRPLHINKAGSSQGDTESPSHEKLVDSTFTPSSYSSTGSNANINNANNTALGRLPRPAGYPSTVSTVEGHGPPLSPAIRVQEVAWKLSSNRERHVPMCEDLELRRDSGSAGTQAHCLLLRKANPSRCHSRESQAAMAGQEETSQDETYEVKMNHDTEACSEPSLLSTEMLSYQDDENRQLTLPEEDKRDIRQSPKRGFLRSASLGRRASFHLECLKRQKDRGGDISQKTVLPLHLVHHQALAVAGLSPLLQRSHSPASFPRPFATPPATPGSRGWPPQPVPTLRLEGVESSEKLNSSFPSIHCGSWAETTPGGGGSSAARRVRPVSLMVPSQAGAPGRQFHGSASSLVEAVLISEGLGQFAQDPKFIEVTTQELADACDMTIEEMESAADNILSGGAPQSPNGALLPFVNCRDAGQDRAGGEEDAGCVRARGRPSEEELQDSRVYVSSL cac1b_rabit Voltage-dependent N-type calcium channel subunit alpha-1B CACNA1B CACH5 CACNL1A5 Brain calcium channel III Calcium channel, L type, alpha-1 polypeptide isoform 5 Voltage-gated calcium channel subunit alpha Cav2.2 protein protein rabit Oryctolagus cuniculus (Rabbit) Rabbit MVRFGDELGGRYGGAGGAERARGGGAGGAGGPGPGGLPPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKILALGFVLHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDPDPVGDFPCGKEAPARLCEGDTECREYWAGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDRNAEEKSPLDAVLKRAAAKKSRSDLIQAEEGEGRLTGLCAPGSPFARASLKSGKTESSSYFRRKEKMFRFFIRRMVKAQSFYWTVLCVVALNTLCVAMVHYNQPQRLTTALYFAEFVFLGLFLTEMSLKMYGLGPRSYFRSSFNCFDFGVIVGSIFEVVWAAVKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFKDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVSRGMFSSFYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEAANQKLALQKAKEVAEVSPMSAANISIAARQQNSAKARSVWEQRASQLRLQNLRASCEALYSEMDPEERLRYATARHLRPDVKTHLDRPLVVEPGRDAPRGPPGGKSRPDGSEAPEGADPPRRHHRHRDKDKAPATVPSAGEQDRAEALRAEGGELGPREERGRPRRSRSKEAPGAPEVRSDRGRGPCPEGGRRHHRRGSPEEAAEREPRRHRAHRHGPDPGKEGPASGTRGERRARHRTGPRACPREAESSEEPARRHRARHKAPPTQETAEKDKEAAEKGGEATEAEKDKEARNHQPKELPCDLEAIGMLGVGAVHTLPSTCLQKVEEQPEDADNQRNVTRMGSQPPDTSTTVHIPVTLTGPPGETTVVPSGNVDLESQAEGKKEVETSDVMRSGPRPIVPYSSMFCLSPTNLLRRCCHYIVTMRYFEMVILVVIALSSIALAAEDPVRTDSPRNNALKYMDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSGSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELERDCRGQYLDYEKEEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPMVLKHSVDATYEEQGPSPGYRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISARPLTRYMPQNKQSFQYKTWTFVVSPPFEYFIMAMIALNTVVLMMKFYDAPYEYELMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIANNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDDTSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSSRACDEHSNASECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYSDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISSEDMTVHFTSTLMALIRTALDIKLAPAGTKQHQCDAELRKEISCVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKTSRDQTQQAPGGLSQLGPVSLFHPLKATLEQTQPALRGARAFLRQKSSASLSNGGAVQTQESGIKESVSWGTQRTQDVLCEARAPLERGHSAEIPVGQPGTLAVDVQMQNMTLSGPDAEPQPGLESQGRAASMPRLAAETQPAPDASPMKRSISTLAPRPHTARLGSTALDRPAPSQAPHHHHHRCHRRRDRKQRSLEKGPSLSADTDGAPDSTVGPGLPTGEGPPGCRRERERRQERGRSQERRQPSSSSSEKHRFYSCDRFGGREPPQPKPSLSSHPTSPTAGQEPGPHPQGSGSVHGSPLLSTSGASTPGRGRRQLPQTPLTPRPSVTYKTANSSPVHFAGAPSGLPAFSPGRLSRGLSEHNALLQRDPLSRPLAPGSRIGSDPYLGQRLDSEAPARALPEDAPAFEETAASNSGRSSRTSYVSSLTSQPPPLRRVPNGYHCTLGLGGGGRARRGCHHPDRDRRC cac1a_rabit Voltage-dependent P/Q-type calcium channel subunit alpha-1A CACNA1A CACH4 CACN3 CACNL1A4 Brain calcium channel I Calcium channel, L type, alpha-1 polypeptide isoform 4 Voltage-gated calcium channel subunit alpha Cav2.1 protein protein rabit Oryctolagus cuniculus (Rabbit) Rabbit MARFGDEMPARYGGGGAGAAAGVVVGAAGGRGAGGSRQGGQPGAQRMYKQSMAQRARTMALYNPIPVRQNCLTVNRSLFLFSEDNVVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDDDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGILATVGTEFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILIFAIIGLEFYMGKFHTTCFEEGTDDIQGESPAPCGTEEPARTCPNGTRCQPYWEGPNNGITQFDNILFAVLTVFQCITMEGWTDLLYNSNDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYMEWISKAEEVILAEDETDVEQRHPFDGALRRATIKKSKTDLLHPEEAEDQLADIASVGSPFARASIKSAKLENSSFFHKKERRMRFYIRRMVKTQAFYWTVLSLVALNTLCVAIVHYNQPEWLSDFLYYAEFIFLGLFMSEMFIKMYGLGTRPYFHSSFNCFDCGVIIGSIFEVIWAVIKPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFDEGTPPTNFDTFPAAIMTVFQILTGEDWNEVMYDGIKSQGGVQGGMVFSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAVNQKLALQKAKEVAEVSPLSAANMSIAMKEQQKNQKPAKSVWEQRTSEMRKQNLLASREALYSEMDPEERWKASYARHLRPDMKTHLDRPLVVDPQENRNNNTNKSRVAEPTVDQRLGQQRAEDFLRKQARHHDRARDPSAHAAAGLDARRPWAGSQEAELSREGPYGRESDHQAREGGLEPPGFWEGEAERGKAGDPHRRHAHRQGVGGSGGSRSGSPRTGTADGEPRRHRVHRRPGEDGPDDKAERRGRHREGSRPARSGEGEAEGPDGGGGGGGERRRRHRHGPPPAYDPDARRDDRERRHRRRKDTQGSGVPVSGPNLSTTRPIQQDLSRQEPPLAEDMDNLKNSRLATAEPVSPHENLSHAGLPQSPAKMGSSTDPAGPTPATAANPQNSTASRRTPNNPGNPSNPGPPKTPENSLIVTNPSTAQTNSAKTARKPDHTTVEIPPACPPPLNHTVVQVNKNANPDPLPKKEDEKKEEVDEGPGEDGPKPMPPYSSMFILSTTNPLRRLCHYILNLRYFEMCILMVIAMSSIALAAEDPVQPNAPRNNVLRYFDYVFTGVFTFEMVIKMIDLGLVLHQGAYFRDLWNILDFIVVSGALVAFAFTGNSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVFNILIVYMLFMFIFAVVAVQLFKGKFFHCTDESKEFEKDCRGKYLLYEKNEVKARDREWKKYEFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATFENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEEYSLEKNERACIDFAISAKPLTRHMPQNKQSFQYRMWQFVVSPPFEYTIMAMIALNTIVLMMKFYGASVAYDNALKVFNIVFTSLFSLECLLKVLAFGILNYFRDAWNIFDFVTVLGSITDILVTEFGNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIGIDMEDEDSDEDEFQITEHNNFRTFFQALMLLFRSATGEAWHNIMLSCLSGKPCDKNSGILTPECGNEFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRVWAEYDPAAWGRMLYRDMYAMLRHMPPPLGLGKNCPARVAYKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGADKQQMDAELRKEMMAIWPNLSQKTLDLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKKLQAMREEQNRTPLMFQRMEPPPDEGGAGQNALPSTQLDPAGGLMAHEDGLKDSPSWVTQRAQEMFQKTGTWSPERAPPADMADSQPKPQSVEMREMSQDGYSDSEHCLPMEGQARAASMPRLPAENQRRRGRPRGSDLSTICDTSPMKRSASVLGPKASRRLDDYSLERVPPEENQRHHPRRRERAHRTSERSLGRYTDVDTGLGTDLSMTTQSGDLPSREREQERGRPKDRKHRPHHHHHHHHHPGRGPGRVSPGVSARRRRRGPVARVRPARAPALAHARARARAPARLLPELRLRRARRPRPRQRRRPRRRRGGGGRALRRAPGPREPLAQDSPGRGPSVCLARAARPAGPQRLLPGPRTGQAPRARLPQKPARSVQRERRGLVLSPPPPPPGELAPRAHPARTPRPGPGDSRSRRGGRRWTASAGKGGGGPRASAPSP cacna1c-cacnb2-3 3167675 N/A 3167678 unspecified role unspecified role unspecified role unspecified role Region 17-138 Region 17-138 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Region 203-425 Region 203-425 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Q8VGC3-2:p.Met245Ala M245A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3167679 unspecified role unspecified role unspecified role unspecified role Region 428-445 Region 428-445 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Q13936:p.Trp440Ala W440A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q13936:p.Tyr437Ala Y437A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q13936:p.Ile441Ala I441A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false cacna1b-cacnb2-1 3167676 N/A 3167680 unspecified role unspecified role unspecified role unspecified role Region 379-396 Region 379-396 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3167678 unspecified role unspecified role unspecified role unspecified role Region 203-425 Region 203-425 binding region binding-associated region binding region mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Region 17-138 Region 17-138 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false cacna1a-cacnb2-1 3167677 N/A 3167681 unspecified role unspecified role unspecified role unspecified role Region 383-400 Region 383-400 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3167678 unspecified role unspecified role unspecified role unspecified role Region 203-425 Region 203-425 binding region binding-associated region binding region mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Region 17-138 Region 17-138 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false