DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ huang-2008-11 in vitro In vitro comigration in sds comigration in sds page comigration in sds autoradiography autoradiography Huang DT., Zhuang M., Ayrault O., Schulman BA. DIP original experiment: DIP-255322X Nature structural & molecular biology 2008 IMEx Identification of conjugation specificity determinants unmasks vestigial preference for ubiquitin within the NEDD8 E2. Huang DT., Zhuang M., Ayrault O., Schulman BA. Only protein-protein interactions imex curation huang-2008-12 in vitro In vitro comigration in sds comigration in sds page comigration in sds predetermined predetermined participant predetermined Huang DT., Zhuang M., Ayrault O., Schulman BA. DIP original experiment: DIP-255329X Nature structural & molecular biology 2008 IMEx Identification of conjugation specificity determinants unmasks vestigial preference for ubiquitin within the NEDD8 E2. Huang DT., Zhuang M., Ayrault O., Schulman BA. Only protein-protein interactions imex curation huang-2008-13 in vitro In vitro comigration in sds comigration in sds page comigration in sds autoradiography autoradiography Huang DT., Zhuang M., Ayrault O., Schulman BA. DIP original experiment: DIP-255331X Nature structural & molecular biology 2008 IMEx Identification of conjugation specificity determinants unmasks vestigial preference for ubiquitin within the NEDD8 E2. Huang DT., Zhuang M., Ayrault O., Schulman BA. Only protein-protein interactions imex curation huang-2008-14 in vitro In vitro comigration in sds comigration in sds page comigration in sds predetermined predetermined participant predetermined Huang DT., Zhuang M., Ayrault O., Schulman BA. DIP original experiment: DIP-255327X Nature structural & molecular biology 2008 IMEx Identification of conjugation specificity determinants unmasks vestigial preference for ubiquitin within the NEDD8 E2. Huang DT., Zhuang M., Ayrault O., Schulman BA. Only protein-protein interactions imex curation huang-2008-15 in vitro In vitro comigration in sds comigration in sds page comigration in sds predetermined predetermined participant predetermined Huang DT., Zhuang M., Ayrault O., Schulman BA. DIP original experiment: DIP-255325X Nature structural & molecular biology 2008 IMEx Identification of conjugation specificity determinants unmasks vestigial preference for ubiquitin within the NEDD8 E2. Huang DT., Zhuang M., Ayrault O., Schulman BA. Only protein-protein interactions imex curation huang-2008-16 in vitro In vitro comigration in sds comigration in sds page comigration in sds autoradiography autoradiography Huang DT., Zhuang M., Ayrault O., Schulman BA. DIP original experiment: DIP-255323X Nature structural & molecular biology 2008 IMEx Identification of conjugation specificity determinants unmasks vestigial preference for ubiquitin within the NEDD8 E2. Huang DT., Zhuang M., Ayrault O., Schulman BA. Only protein-protein interactions imex curation huang-2008-17 in vitro In vitro comigration in sds comigration in sds page comigration in sds predetermined predetermined participant predetermined Huang DT., Zhuang M., Ayrault O., Schulman BA. DIP original experiment: DIP-255328X Nature structural & molecular biology 2008 IMEx Identification of conjugation specificity determinants unmasks vestigial preference for ubiquitin within the NEDD8 E2. Huang DT., Zhuang M., Ayrault O., Schulman BA. Only protein-protein interactions imex curation huang-2008-18 in vitro In vitro comigration in sds comigration in sds page comigration in sds predetermined predetermined participant predetermined Huang DT., Zhuang M., Ayrault O., Schulman BA. DIP original experiment: DIP-255330X Nature structural & molecular biology 2008 IMEx Identification of conjugation specificity determinants unmasks vestigial preference for ubiquitin within the NEDD8 E2. Huang DT., Zhuang M., Ayrault O., Schulman BA. Only protein-protein interactions imex curation huang-2008-19 in vitro In vitro comigration in sds comigration in sds page comigration in sds predetermined predetermined participant predetermined Huang DT., Zhuang M., Ayrault O., Schulman BA. DIP original experiment: DIP-255326X Nature structural & molecular biology 2008 IMEx Identification of conjugation specificity determinants unmasks vestigial preference for ubiquitin within the NEDD8 E2. Huang DT., Zhuang M., Ayrault O., Schulman BA. Only protein-protein interactions imex curation huang-2008-20 in vitro In vitro comigration in sds comigration in sds page comigration in sds predetermined predetermined participant predetermined Huang DT., Zhuang M., Ayrault O., Schulman BA. DIP original experiment: DIP-255324X Nature structural & molecular biology 2008 IMEx Identification of conjugation specificity determinants unmasks vestigial preference for ubiquitin within the NEDD8 E2. Huang DT., Zhuang M., Ayrault O., Schulman BA. Only protein-protein interactions imex curation nedd8_human NEDD8 Ubiquitin-like protein Nedd8 Neddylin Neural precursor cell expressed developmentally down-regulated protein 8 NEDD8 protein protein human Homo sapiens Human MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGGGGLRQ ubc12_human NEDD8-conjugating enzyme Ubc12 UBE2M UBC12 Ubiquitin-conjugating enzyme E2 M NEDD8 carrier protein protein protein human Homo sapiens Human MIKLFSLKQQKKEEESAGGTKGSSKKASAAQLRIQKDINELNLPKTCDISFSDPDDLLNFKLVICPDEGFYKSGKFVFSFKVGQGYPHDPPKVKCETMVYHPNIDLEGNVCLNILREDWKPVLTINSIIYGLQYLFLEPNPEDPLNKEAAEVLQNNRRLFEQNVQRSMRGGYIGSTYFERCLK cul1_human Cullin-1 CUL1 protein protein human Homo sapiens Human MSSTRSQNPHGLKQIGLDQIWDDLRAGIQQVYTRQSMAKSRYMELYTHVYNYCTSVHQSNQARGAGVPPSKSKKGQTPGGAQFVGLELYKRLKEFLKNYLTNLLKDGEDLMDESVLKFYTQQWEDYRFSSKVLNGICAYLNRHWVRRECDEGRKGIYEIYSLALVTWRDCLFRPLNKQVTNAVLKLIEKERNGETINTRLISGVVQSYVELGLNEDDAFAKGPTLTVYKESFESQFLADTERFYTRESTEFLQQNPVTEYMKKAEARLLEEQRRVQVYLHESTQDELARKCEQVLIEKHLEIFHTEFQNLLDADKNEDLGRMYNLVSRIQDGLGELKKLLETHIHNQGLAAIEKCGEAALNDPKMYVQTVLDVHKKYNALVMSAFNNDAGFVAALDKACGRFINNNAVTKMAQSSSKSPELLARYCDSLLKKSSKNPEEAELEDTLNQVMVVFKYIEDKDVFQKFYAKMLAKRLVHQNSASDDAEASMISKLKQACGFEYTSKLQRMFQDIGVSKDLNEQFKKHLTNSEPLDLDFSIQVLSSGSWPFQQSCTFALPSELERSYQRFTAFYASRHSGRKLTWLYQLSKGELVTNCFKNRYTLQASTFQMAILLQYNTEDAYTVQQLTDSTQIKMDILAQVLQILLKSKLLVLEDENANVDEVELKPDTLIKLYLGYKNKKLRVNINVPMKTEQKQEQETTHKNIEEDRKLLIQAAIVRIMKMRKVLKHQQLLGEVLTQLSSRFKPRVPVIKKCIDILIEKEYLERVDGEKDTYSYLA sumo1_human Small ubiquitin-related modifier 1 SUMO1 Sentrin Ubiquitin-like protein SMT3C Ubiquitin-homology domain protein PIC1 Ubiquitin-like protein UBL1 GAP-modifying protein 1 SMT3 homolog 3 SMT3C SMT3H3 UBL1 OK/SW-cl.43 protein protein human Homo sapiens Human MSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGGHSTV ubc9_human SUMO-conjugating enzyme UBC9 UBE2I p18 SUMO-protein ligase UBC9 UBCE9 Ubiquitin carrier protein 9 Ubiquitin carrier protein I Ubiquitin-conjugating enzyme E2 I Ubiquitin-protein ligase I RING-type E3 SUMO transferase UBC9 protein protein human Homo sapiens Human MSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFAPS ube2k_human Ubiquitin-conjugating enzyme E2 K HIP2 Ubiquitin-conjugating enzyme E2-25 kDa Ubiquitin-protein ligase Ubiquitin carrier protein Huntingtin-interacting protein 2 UBE2K LIG E2 ubiquitin-conjugating enzyme K protein protein human Homo sapiens Human MANIAVQRIKREFKEVLKSEETSKNQIKVDLVDENFTELRGEIAGPPDTPYEGGRYQLEIKIPETYPFNPPKVRFITKIWHPNISSVTGAICLDILKDQWAAAMTLRTVLLSLQALLAAAEPDDPQDAVVANQYKQNPEMFKQTARLWAHVYAGAPVSSPEYTKKIENLCAMGFDRNAVIVALSSKSWDVETATELLLSN ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG ub2l6_human Ubiquitin/ISG15-conjugating enzyme E2 L6 UBE2L6 UBCH8 Ubiquitin-protein ligase L6 Ubiquitin carrier protein L6 UbcH8 Retinoic acid-induced gene B protein E2 ubiquitin-conjugating enzyme L6 protein protein human Homo sapiens Human MMASMRVVKELEDLQKKPPPYLRNLSSDDANVLVWHALLLPDQPPYHLKAFNLRISFPPEYPFKPPMIKFTTKIYHPNVDENGQICLPIISSENWKPCTKTCQVLEALNVLVNRPNIREPLRMDLADLLTQNPELFRKNAEEFTLRFGVDRPS ub2d2_human Ubiquitin-conjugating enzyme E2 D2 UBC5B UBCH4 UBE2D2 UBC4 UBCH5B Ubiquitin-protein ligase D2 Ubiquitin carrier protein D2 Ubiquitin-conjugating enzyme E2-17 kDa 2 Ubiquitin-conjugating enzyme E2(17)KB 2 PUBC1 p53-regulated ubiquitin-conjugating enzyme 1 (E3-independent) E2 ubiquitin-conjugating enzyme D2 E2 ubiquitin-conjugating enzyme D2 protein protein human Homo sapiens Human MALKRIHKELNDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDREKYNRIAREWTQKYAM ubc2_yeast Ubiquitin-conjugating enzyme E2 2 RAD6 UBC2 Ubiquitin-protein ligase UBC2 Ubiquitin carrier protein UBC2 Radiation sensitivity protein 6 YGL058W E2 ubiquitin-conjugating enzyme 2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSTPARRRLMRDFKRMKEDAPPGVSASPLPDNVMVWNAMIIGPADTPYEDGTFRLLLEFDEEYPNKPPHVKFLSEMFHPNVYANGEICLDILQNRWTPTYDVASILTSIQSLFNDPNPASPANVEAATLFKDHKSQYVKRVKETVEKSWEDDMDDMDDDDDDDDDDDDDEAD 5F568DC28ABBD60F o00762-1 Ubiquitin-conjugating enzyme E2 C UBE2C UBCH10 (E3-independent) E2 ubiquitin-conjugating enzyme C E2 ubiquitin-conjugating enzyme C UbcH10 Ubiquitin carrier protein C Ubiquitin-protein ligase C protein protein human Homo sapiens Human MASQNRDPAATSVAAARKGAEPSGGAARGPVGKRLQQELMTLMMSGDKGISAFPESDNLFKWVGTIHGAAGTVYEDLRYKLSLEFPSGYPYNAPTVKFLTPCYHPNVDTQGNICLDILKEKWSALYDVRTILLSIQSLLGEPNIDSPLNTHAAELWKNPTAFKKYLQETYSKQVTSQEP p68036-1 Ubiquitin-conjugating enzyme E2 L3 E2 ubiquitin-conjugating enzyme L3 L-UBC UbcH7 Ubiquitin carrier protein L3 Ubiquitin-conjugating enzyme E2-F1 Ubiquitin-protein ligase L3 UBE2L3 UBCE7 UBCH7 protein protein human Homo sapiens Human MAASRRLMKELEEIRKCGMKNFRNIQVDEANLLTWQGLIVPDNPPYDKGAFRIEINFPAEYPFKPPKITFKTKIYHPNIDEKGQVCLPVISAENWKPATKTDQVIQSLIALVNDPQPEHPLRADLAEEYSKDRKKFCKNAEEFTKKYGEKRPVD nedd8-ube2m-2 3167321 N/A 3167331 unspecified role unspecified role unspecified role unspecified role 32P-label 32P-label 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli N/A 3167332 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false cul1-nedd8-2 3167322 N/A 3167333 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3167331 unspecified role unspecified role unspecified role unspecified role 32P-label 32P-label 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli direct interaction direct interaction false false false sumo1-ube2i-7 3167323 N/A 3167334 unspecified role unspecified role unspecified role unspecified role 32P-label 32P-label 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli N/A 3167335 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false ub-ube2k-1 3167324 N/A 3167336 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3167337 unspecified role unspecified role unspecified role unspecified role 32P-label 32P-label 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli direct interaction direct interaction false false false ub-ube2l6-1 3167325 N/A 3167338 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3167337 unspecified role unspecified role unspecified role unspecified role 32P-label 32P-label 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli direct interaction direct interaction false false false ub-ube2d2-1 3167326 N/A 3167337 unspecified role unspecified role unspecified role unspecified role 32P-label 32P-label 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli N/A 3167339 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false ub-ubc2p-1 3167327 N/A 3167340 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3167337 unspecified role unspecified role unspecified role unspecified role 32P-label 32P-label 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli direct interaction direct interaction false false false cul1-ub-1 3167328 N/A 3167333 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3167337 unspecified role unspecified role unspecified role unspecified role 32P-label 32P-label 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli direct interaction direct interaction false false false ub-ube2c-1 3167329 N/A 3167341 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3167337 unspecified role unspecified role unspecified role unspecified role 32P-label 32P-label 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli direct interaction direct interaction false false false ub-ube2l3-1 3167330 N/A 3167342 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3167337 unspecified role unspecified role unspecified role unspecified role 32P-label 32P-label 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli direct interaction direct interaction false false false