DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ jia-2008-1 arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Jia G., Liu X., Owen HA., Zhao D. DIP original experiment: DIP-68275X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Signaling of cell fate determination by the TPD1 small protein and EMS1 receptor kinase. Jia G., Liu X., Owen HA., Zhao D. Only protein-protein interactions imex curation jia-2008-2 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Jia G., Liu X., Owen HA., Zhao D. DIP original experiment: DIP-68273X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Signaling of cell fate determination by the TPD1 small protein and EMS1 receptor kinase. Jia G., Liu X., Owen HA., Zhao D. Only protein-protein interactions imex curation jia-2008-3 arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Jia G., Liu X., Owen HA., Zhao D. DIP original experiment: DIP-68276X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Signaling of cell fate determination by the TPD1 small protein and EMS1 receptor kinase. Jia G., Liu X., Owen HA., Zhao D. Only protein-protein interactions imex curation jia-2008-4 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Jia G., Liu X., Owen HA., Zhao D. DIP original experiment: DIP-68274X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Signaling of cell fate determination by the TPD1 small protein and EMS1 receptor kinase. Jia G., Liu X., Owen HA., Zhao D. Only protein-protein interactions imex curation ems1_arath Leucine-rich repeat receptor protein kinase EMS1 EMS1 ESP EXS Protein EXCESS MICROSPOROCYTES 1 Protein EXTRA SPOROGENOUS CELLS T28J14.220 At5g07280 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAFLTALFLFLFFSFSSSAIVDLSSETTSLISFKRSLENPSLLSSWNVSSSASHCDWVGVTCLLGRVNSLSLPSLSLRGQIPKEISSLKNLRELCLAGNQFSGKIPPEIWNLKHLQTLDLSGNSLTGLLPRLLSELPQLLYLDLSDNHFSGSLPPSFFISLPALSSLDVSNNSLSGEIPPEIGKLSNLSNLYMGLNSFSGQIPSEIGNISLLKNFAAPSCFFNGPLPKEISKLKHLAKLDLSYNPLKCSIPKSFGELHNLSILNLVSAELIGLIPPELGNCKSLKSLMLSFNSLSGPLPLELSEIPLLTFSAERNQLSGSLPSWMGKWKVLDSLLLANNRFSGEIPHEIEDCPMLKHLSLASNLLSGSIPRELCGSGSLEAIDLSGNLLSGTIEEVFDGCSSLGELLLTNNQINGSIPEDLWKLPLMALDLDSNNFTGEIPKSLWKSTNLMEFTASYNRLEGYLPAEIGNAASLKRLVLSDNQLTGEIPREIGKLTSLSVLNLNANMFQGKIPVELGDCTSLTTLDLGSNNLQGQIPDKITALAQLQCLVLSYNNLSGSIPSKPSAYFHQIEMPDLSFLQHHGIFDLSYNRLSGPIPEELGECLVLVEISLSNNHLSGEIPASLSRLTNLTILDLSGNALTGSIPKEMGNSLKLQGLNLANNQLNGHIPESFGLLGSLVKLNLTKNKLDGPVPASLGNLKELTHMDLSFNNLSGELSSELSTMEKLVGLYIEQNKFTGEIPSELGNLTQLEYLDVSENLLSGEIPTKICGLPNLEFLNLAKNNLRGEVPSDGVCQDPSKALLSGNKELCGRVVGSDCKIEGTKLRSAWGIAGLMLGFTIIVFVFVFSLRRWAMTKRVKQRDDPERMEESRLKGFVDQNLYFLSGSRSREPLSINIAMFEQPLLKVRLGDIVEATDHFSKKNIIGDGGFGTVYKACLPGEKTVAVKKLSEAKTQGNREFMAEMETLGKVKHPNLVSLLGYCSFSEEKLLVYEYMVNGSLDHWLRNQTGMLEVLDWSKRLKIAVGAARGLAFLHHGFIPHIIHRDIKASNILLDGDFEPKVADFGLARLISACESHVSTVIAGTFGYIPPEYGQSARATTKGDVYSFGVILLELVTGKEPTGPDFKESEGGNLVGWAIQKINQGKAVDVIDPLLVSVALKNSQLRLLQIAMLCLAETPAKRPNMLDVLKALKEI tpd1_arath Protein TAPETUM DETERMINANT 1 TPD1 At4g24972 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MNRRRLLVSATLLSYLLYGMALVSVEASGGEKLRDNLDLTKTTTSSPSISHRKMLLLSPGTGKTERSVEPERIGEKCKSTDIVVNQAVTEPMPNGIPGYMVEITNQCMSGCIISRIHINCGWFSSAKLINPRVFKRIHYDDCLVNNGKPLPFGSTLSFHYANTFPYHLSVAFVTCA ems1-tpd1-1 3167209 N/A 3167213 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress N/A 3167214 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress physical association physical association false false false ems1-tpd1-2 3167210 N/A 3167213 unspecified role unspecified role bait bait Binding region 352-467 Binding region 352-467 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3167214 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast direct interaction direct interaction false false false tpd1-ems1-1 3167211 N/A 3167213 unspecified role unspecified role prey prey Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress N/A 3167214 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress physical association physical association false false false ems1-tpd1-3 3167212 N/A 3167214 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3167213 unspecified role unspecified role bait bait Binding region 352-467 Binding region 352-467 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false