DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ mori-2007-5 ecolx Escherichia coli fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Mori T., Vale RD., Tomishige M. DIP original experiment: DIP-253782X Nature 2007 IMEx How kinesin waits between steps. Mori T., Vale RD., Tomishige M. Only protein-protein interactions imex curation mori-2007-6 ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Mori T., Vale RD., Tomishige M. DIP original experiment: DIP-253784X Nature 2007 IMEx How kinesin waits between steps. Mori T., Vale RD., Tomishige M. Only protein-protein interactions imex curation mori-2007-7 ecolx Escherichia coli fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Mori T., Vale RD., Tomishige M. DIP original experiment: DIP-253783X Nature 2007 IMEx How kinesin waits between steps. Mori T., Vale RD., Tomishige M. Only protein-protein interactions imex curation mori-2007-8 ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Mori T., Vale RD., Tomishige M. DIP original experiment: DIP-253785X Nature 2007 IMEx How kinesin waits between steps. Mori T., Vale RD., Tomishige M. Only protein-protein interactions imex curation kinh_human Kinesin-1 heavy chain KIF5B KNS KNS1 Ubiquitous kinesin heavy chain Conventional kinesin heavy chain protein protein human Homo sapiens Human MADLAECNIKVMCRFRPLNESEVNRGDKYIAKFQGEDTVVIASKPYAFDRVFQSSTSQEQVYNDCAKKIVKDVLEGYNGTIFAYGQTSSGKTHTMEGKLHDPEGMGIIPRIVQDIFNYIYSMDENLEFHIKVSYFEIYLDKIRDLLDVSKTNLSVHEDKNRVPYVKGCTERFVCSPDEVMDTIDEGKSNRHVAVTNMNEHSSRSHSIFLINVKQENTQTEQKLSGKLYLVDLAGSEKVSKTGAEGAVLDEAKNINKSLSALGNVISALAEGSTYVPYRDSKMTRILQDSLGGNCRTTIVICCSPSSYNESETKSTLLFGQRAKTIKNTVCVNVELTAEQWKKKYEKEKEKNKILRNTIQWLENELNRWRNGETVPIDEQFDKEKANLEAFTVDKDITLTNDKPATAIGVIGNFTDAERRKCEEEIAKLYKQLDDKDEEINQQSQLVEKLKTQMLDQEELLASTRRDQDNMQAELNRLQAENDASKEEVKEVLQALEELAVNYDQKSQEVEDKTKEYELLSDELNQKSATLASIDAELQKLKEMTNHQKKRAAEMMASLLKDLAEIGIAVGNNDVKQPEGTGMIDEEFTVARLYISKMKSEVKTMVKRCKQLESTQTESNKKMEENEKELAACQLRISQHEAKIKSLTEYLQNVEQKKRQLEESVDALSEELVQLRAQEKVHEMEKEHLNKVQTANEVKQAVEQQIQSHRETHQKQISSLRDEVEAKAKLITDLQDQNQKMMLEQERLRVEHEKLKATDQEKSRKLHELTVMQDRREQARQDLKGLEETVAKELQTLHNLRKLFVQDLATRVKKSAEIDSDDTGGSAAQKQKISFLENNLEQLTKVHKQLVRDNADLRCELPKLEKRLRATAERVKALESALKEAKENASRDRKRYQQEVDRIKEAVRSKNMARRGHSAQIAKPIRPGQHPAASPTHPSAIRGGGAFVQNSQPVAVRGGGGKQV kif5b-1 3164311 N/A 3164315 unspecified role unspecified role fluorescence donor fluorescence donor Region 1-490 Region 1-490 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Cy3-label Cy3-label cy3 label cy3 label certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3164315 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept Region 1-490 Region 1-490 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Cy5-label Cy5-label cy5 label cy5 label certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false kif5b-kif5b-2 3164312 N/A 3164315 unspecified role unspecified role prey prey Region 1-490 Region 1-490 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecolx Escherichia coli N/A 3164315 unspecified role unspecified role bait bait Region 1-490 Region 1-490 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Strep-tag Strep-tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecolx Escherichia coli physical association physical association false false false kif5b-2 3164313 N/A 3164315 unspecified role unspecified role fluorescence donor fluorescence donor Region 1-490 Region 1-490 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Cy3-label Cy3-label cy3 label cy3 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli N/A 3164315 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept Region 1-490 Region 1-490 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Cy5-label Cy5-label cy5 label cy5 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli direct interaction direct interaction false false false kif5b-kif5b-3 3164314 N/A 3164315 unspecified role unspecified role bait bait Region 1-490 Region 1-490 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecolx Escherichia coli N/A 3164315 unspecified role unspecified role prey prey Region 1-490 Region 1-490 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Strep-tag Strep-tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecolx Escherichia coli physical association physical association false false false