DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ boularan-2007-1 human Homo sapiens Human fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Boularan C., Scott MG., Bourougaa K., Bellal M., Esteve E., Thuret A., Benmerah A., Tramier M., Coppey-Moisan M., Labbé-Jullié C., Fåhraeus R., Marullo S. DIP original experiment: DIP-261866X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx beta-arrestin 2 oligomerization controls the Mdm2-dependent inhibition of p53. Boularan C., Scott MG., Bourougaa K., Bellal M., Esteve E., Thuret A., Benmerah A., Tramier M., Coppey-Moisan M., Labbé-Jullié C., Fåhraeus R., Marullo S. Only protein-protein interactions imex curation boularan-2007-2 chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Boularan C., Scott MG., Bourougaa K., Bellal M., Esteve E., Thuret A., Benmerah A., Tramier M., Coppey-Moisan M., Labbé-Jullié C., Fåhraeus R., Marullo S. DIP original experiment: DIP-261861X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx beta-arrestin 2 oligomerization controls the Mdm2-dependent inhibition of p53. Boularan C., Scott MG., Bourougaa K., Bellal M., Esteve E., Thuret A., Benmerah A., Tramier M., Coppey-Moisan M., Labbé-Jullié C., Fåhraeus R., Marullo S. Only protein-protein interactions imex curation boularan-2007-3 human Homo sapiens Human bret bioluminescence resonance energy transfer BRET LRET LRET BRET bret predetermined predetermined participant predetermined Boularan C., Scott MG., Bourougaa K., Bellal M., Esteve E., Thuret A., Benmerah A., Tramier M., Coppey-Moisan M., Labbé-Jullié C., Fåhraeus R., Marullo S. DIP original experiment: DIP-261864X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx beta-arrestin 2 oligomerization controls the Mdm2-dependent inhibition of p53. Boularan C., Scott MG., Bourougaa K., Bellal M., Esteve E., Thuret A., Benmerah A., Tramier M., Coppey-Moisan M., Labbé-Jullié C., Fåhraeus R., Marullo S. Only protein-protein interactions imex curation boularan-2007-4 human Homo sapiens Human bret bioluminescence resonance energy transfer BRET LRET LRET BRET bret predetermined predetermined participant predetermined Boularan C., Scott MG., Bourougaa K., Bellal M., Esteve E., Thuret A., Benmerah A., Tramier M., Coppey-Moisan M., Labbé-Jullié C., Fåhraeus R., Marullo S. DIP original experiment: DIP-261863X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx beta-arrestin 2 oligomerization controls the Mdm2-dependent inhibition of p53. Boularan C., Scott MG., Bourougaa K., Bellal M., Esteve E., Thuret A., Benmerah A., Tramier M., Coppey-Moisan M., Labbé-Jullié C., Fåhraeus R., Marullo S. Only protein-protein interactions imex curation boularan-2007-5 chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Boularan C., Scott MG., Bourougaa K., Bellal M., Esteve E., Thuret A., Benmerah A., Tramier M., Coppey-Moisan M., Labbé-Jullié C., Fåhraeus R., Marullo S. DIP original experiment: DIP-261867X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx beta-arrestin 2 oligomerization controls the Mdm2-dependent inhibition of p53. Boularan C., Scott MG., Bourougaa K., Bellal M., Esteve E., Thuret A., Benmerah A., Tramier M., Coppey-Moisan M., Labbé-Jullié C., Fåhraeus R., Marullo S. Only protein-protein interactions imex curation boularan-2007-6 human Homo sapiens Human fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Boularan C., Scott MG., Bourougaa K., Bellal M., Esteve E., Thuret A., Benmerah A., Tramier M., Coppey-Moisan M., Labbé-Jullié C., Fåhraeus R., Marullo S. DIP original experiment: DIP-261865X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx beta-arrestin 2 oligomerization controls the Mdm2-dependent inhibition of p53. Boularan C., Scott MG., Bourougaa K., Bellal M., Esteve E., Thuret A., Benmerah A., Tramier M., Coppey-Moisan M., Labbé-Jullié C., Fåhraeus R., Marullo S. Only protein-protein interactions imex curation boularan-2007-7 chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Boularan C., Scott MG., Bourougaa K., Bellal M., Esteve E., Thuret A., Benmerah A., Tramier M., Coppey-Moisan M., Labbé-Jullié C., Fåhraeus R., Marullo S. DIP original experiment: DIP-261862X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx beta-arrestin 2 oligomerization controls the Mdm2-dependent inhibition of p53. Boularan C., Scott MG., Bourougaa K., Bellal M., Esteve E., Thuret A., Benmerah A., Tramier M., Coppey-Moisan M., Labbé-Jullié C., Fåhraeus R., Marullo S. Only protein-protein interactions imex curation arrb2_rat Beta-arrestin-2 Arrb2 Arrestin beta-2 protein protein rat Rattus norvegicus (Rat) Rat MGEKPGTRVFKKSSPNCKLTVYLGKRDFVDHLDKVDPVDGVVLVDPDYLKDRKVFVTLTCAFRYGREDLDVLGLSFRKDLFIATYQAFPPMPNPPRPPTRLQDRLLKKLGQHAHPFFFTIPQNLPCSVTLQPGPEDTGKACGVDFEIRAFCAKSIEEKSHKRNSVRLIIRKVQFAPETPGPQPSAETTRHFLMSDRRSLHLEASLDKELYYHGEPLNVNVHVTNNSAKTVKKIRVSVRQYADICLFSTAQYKCPVAQLEQDDQVSPSSTFCKVYTITPLLSDNREKRGLALDGQLKHEDTNLASSTIVKEGANKEVLGILVSYRVKVKLVVSRGGDVSVELPFVLMHPKPHDHITLPRPQSAPREIDIPVDTNLIEFDTNYATDDDIVFEDFARLRLKGMKDDDCDDQFC mdm2_human E3 ubiquitin-protein ligase Mdm2 MDM2 p53-binding protein Mdm2 Oncoprotein Mdm2 Double minute 2 protein RING-type E3 ubiquitin transferase Mdm2 protein protein human Homo sapiens Human MCNTNMSVPTDGAVTTSQIPASEQETLVRPKPLLLKLLKSVGAQKDTYTMKEVLFYLGQYIMTKRLYDEKQQHIVYCSNDLLGDLFGVPSFSVKEHRKIYTMIYRNLVVVNQQESSDSGTSVSENRCHLEGGSDQKDLVQELQEEKPSSSHLVSRPSTSSRRRAISETEENSDELSGERQRKRHKSDSISLSFDESLALCVIREICCERSSSSESTGTPSNPDLDAGVSEHSGDWLDQDSVSDQFSVEFEVESLDSEDYSLSEEGQELSDEDDEVYQVTVYQAGESDTDSFEEDPEISLADYWKCTSCNEMNPPLPSHCNRCWALRENWLPEDKGKDKGEISEKAKLENSTQAEEGFDVPDCKKTIVNDSRESCVEENDDKITQASQSQESEDYSQPSTSSSIIYSSQEDVKEFEREETQDKEESVESSLPLNAIEPCVICQGRPKNGCIVHGKTGHLMACFTCAKKLKKRNKPCPVCRQPIQMIVLTYFP flna_human Filamin-A FLNA FLN FLN1 Alpha-filamin Filamin-1 Endothelial actin-binding protein Actin-binding protein 280 Non-muscle filamin protein protein human Homo sapiens Human MSSSHSRAGQSAAGAAPGGGVDTRDAEMPATEKDLAEDAPWKKIQQNTFTRWCNEHLKCVSKRIANLQTDLSDGLRLIALLEVLSQKKMHRKHNQRPTFRQMQLENVSVALEFLDRESIKLVSIDSKAIVDGNLKLILGLIWTLILHYSISMPMWDEEEDEEAKKQTPKQRLLGWIQNKLPQLPITNFSRDWQSGRALGALVDSCAPGLCPDWDSWDASKPVTNAREAMQQADDWLGIPQVITPEEIVDPNVDEHSVMTYLSQFPKAKLKPGAPLRPKLNPKKARAYGPGIEPTGNMVKKRAEFTVETRSAGQGEVLVYVEDPAGHQEEAKVTANNDKNRTFSVWYVPEVTGTHKVTVLFAGQHIAKSPFEVYVDKSQGDASKVTAQGPGLEPSGNIANKTTYFEIFTAGAGTGEVEVVIQDPMGQKGTVEPQLEARGDSTYRCSYQPTMEGVHTVHVTFAGVPIPRSPYTVTVGQACNPSACRAVGRGLQPKGVRVKETADFKVYTKGAGSGELKVTVKGPKGEERVKQKDLGDGVYGFEYYPMVPGTYIVTITWGGQNIGRSPFEVKVGTECGNQKVRAWGPGLEGGVVGKSADFVVEAIGDDVGTLGFSVEGPSQAKIECDDKGDGSCDVRYWPQEAGEYAVHVLCNSEDIRLSPFMADIRDAPQDFHPDRVKARGPGLEKTGVAVNKPAEFTVDAKHGGKAPLRVQVQDNEGCPVEALVKDNGNGTYSCSYVPRKPVKHTAMVSWGGVSIPNSPFRVNVGAGSHPNKVKVYGPGVAKTGLKAHEPTYFTVDCAEAGQGDVSIGIKCAPGVVGPAEADIDFDIIRNDNDTFTVKYTPRGAGSYTIMVLFADQATPTSPIRVKVEPSHDASKVKAEGPGLSRTGVELGKPTHFTVNAKAAGKGKLDVQFSGLTKGDAVRDVDIIDHHDNTYTVKYTPVQQGPVGVNVTYGGDPIPKSPFSVAVSPSLDLSKIKVSGLGEKVDVGKDQEFTVKSKGAGGQGKVASKIVGPSGAAVPCKVEPGLGADNSVVRFLPREEGPYEVEVTYDGVPVPGSPFPLEAVAPTKPSKVKAFGPGLQGGSAGSPARFTIDTKGAGTGGLGLTVEGPCEAQLECLDNGDGTCSVSYVPTEPGDYNINILFADTHIPGSPFKAHVVPCFDASKVKCSGPGLERATAGEVGQFQVDCSSAGSAELTIEICSEAGLPAEVYIQDHGDGTHTITYIPLCPGAYTVTIKYGGQPVPNFPSKLQVEPAVDTSGVQCYGPGIEGQGVFREATTEFSVDARALTQTGGPHVKARVANPSGNLTETYVQDRGDGMYKVEYTPYEEGLHSVDVTYDGSPVPSSPFQVPVTEGCDPSRVRVHGPGIQSGTTNKPNKFTVETRGAGTGGLGLAVEGPSEAKMSCMDNKDGSCSVEYIPYEAGTYSLNVTYGGHQVPGSPFKVPVHDVTDASKVKCSGPGLSPGMVRANLPQSFQVDTSKAGVAPLQVKVQGPKGLVEPVDVVDNADGTQTVNYVPSREGPYSISVLYGDEEVPRSPFKVKVLPTHDASKVKASGPGLNTTGVPASLPVEFTIDAKDAGEGLLAVQITDPEGKPKKTHIQDNHDGTYTVAYVPDVTGRYTILIKYGGDEIPFSPYRVRAVPTGDASKCTVTVSIGGHGLGAGIGPTIQIGEETVITVDTKAAGKGKVTCTVCTPDGSEVDVDVVENEDGTFDIFYTAPQPGKYVICVRFGGEHVPNSPFQVTALAGDQPSVQPPLRSQQLAPQYTYAQGGQQTWAPERPLVGVNGLDVTSLRPFDLVIPFTIKKGEITGEVRMPSGKVAQPTITDNKDGTVTVRYAPSEAGLHEMDIRYDNMHIPGSPLQFYVDYVNCGHVTAYGPGLTHGVVNKPATFTVNTKDAGEGGLSLAIEGPSKAEISCTDNQDGTCSVSYLPVLPGDYSILVKYNEQHVPGSPFTARVTGDDSMRMSHLKVGSAADIPINISETDLSLLTATVVPPSGREEPCLLKRLRNGHVGISFVPKETGEHLVHVKKNGQHVASSPIPVVISQSEIGDASRVRVSGQGLHEGHTFEPAEFIIDTRDAGYGGLSLSIEGPSKVDINTEDLEDGTCRVTYCPTEPGNYIINIKFADQHVPGSPFSVKVTGEGRVKESITRRRRAPSVANVGSHCDLSLKIPEISIQDMTAQVTSPSGKTHEAEIVEGENHTYCIRFVPAEMGTHTVSVKYKGQHVPGSPFQFTVGPLGEGGAHKVRAGGPGLERAEAGVPAEFSIWTREAGAGGLAIAVEGPSKAEISFEDRKDGSCGVAYVVQEPGDYEVSVKFNEEHIPDSPFVVPVASPSGDARRLTVSSLQESGLKVNQPASFAVSLNGAKGAIDAKVHSPSGALEECYVTEIDQDKYAVRFIPRENGVYLIDVKFNGTHIPGSPFKIRVGEPGHGGDPGLVSAYGAGLEGGVTGNPAEFVVNTSNAGAGALSVTIDGPSKVKMDCQECPEGYRVTYTPMAPGSYLISIKYGGPYHIGGSPFKAKVTGPRLVSNHSLHETSSVFVDSLTKATCAPQHGAPGPGPADASKVVAKGLGLSKAYVGQKSSFTVDCSKAGNNMLLVGVHGPRTPCEEILVKHVGSRLYSVSYLLKDKGEYTLVVKWGDEHIPGSPYRVVVP arrb2-1 3163807 N/A 3163814 unspecified role unspecified role fluorescence donor fluorescence donor EGFP-tag EGFP-tag egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3163814 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept mCherry-tag mCherry-tag mcherry mcherry fluorescent protein tag mcherry c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human Homo sapiens Human proximity proximity close-contact colocalization neighbourhood interaction false false false arrb2-arrb2-1 3163808 N/A 3163814 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false P29067:p.[Lys158Ala;Lys161Ala;Arg162Ala] K158A/K161A/R162A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P29067:p.[Lys232Ala;Arg234Ala;Lys252Ala;Lys326Ala;Lys328Ala] K232A/R234A/K252A/K326A/K328A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey N/A 3163814 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false P29067:p.[Lys158Ala;Lys161Ala;Arg162Ala] K158A/K161A/R162A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P29067:p.[Lys232Ala;Arg234Ala;Lys252Ala;Lys326Ala;Lys328Ala] K232A/R234A/K252A/K326A/K328A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey physical association physical association false false false arrb2-2 3163809 N/A 3163814 unspecified role unspecified role fluorescence donor fluorescence donor RLuc-tag RLuc-tag renilla luciferase renilla luciferase protein tag renilla luciferase c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human Homo sapiens Human N/A 3163814 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept YFP-tag YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false P29067:p.[Lys158Ala;Lys161Ala;Arg162Ala] K158A/K161A/R162A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P29067:p.[Lys232Ala;Arg234Ala;Lys252Ala;Lys326Ala;Lys328Ala] K232A/R234A/K252A/K326A/K328A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human proximity proximity close-contact colocalization neighbourhood interaction false false false mdm2-arrb2-3 3163810 N/A 3163814 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept YFP-tag YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false P29067:p.[Lys158Ala;Lys161Ala;Arg162Ala] K158A/K161A/R162A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P29067:p.[Lys232Ala;Arg234Ala;Lys252Ala;Lys326Ala;Lys328Ala] K232A/R234A/K252A/K326A/K328A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human N/A 3163815 unspecified role unspecified role fluorescence donor fluorescence donor RLuc-tag RLuc-tag renilla luciferase renilla luciferase protein tag renilla luciferase n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human proximity proximity close-contact colocalization neighbourhood interaction false false false arrb2-flna-1 3163811 N/A 3163816 unspecified role unspecified role prey prey Region 2363-2647 Region 2363-2647 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey N/A 3163814 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey physical association physical association false false false mdm2-arrb2-4 3163812 N/A 3163814 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept mCherry-tag mCherry-tag mcherry mcherry fluorescent protein tag mcherry c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human Homo sapiens Human N/A 3163815 unspecified role unspecified role fluorescence donor fluorescence donor EGFP-tag EGFP-tag egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human proximity proximity close-contact colocalization neighbourhood interaction false false false arrb2-mdm2-2 3163813 N/A 3163815 unspecified role unspecified role prey prey RLuc-tag RLuc-tag renilla luciferase renilla luciferase protein tag renilla luciferase n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey N/A 3163814 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false P29067:p.[Lys158Ala;Lys161Ala;Arg162Ala] K158A/K161A/R162A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P29067:p.[Lys232Ala;Arg234Ala;Lys252Ala;Lys326Ala;Lys328Ala] K232A/R234A/K252A/K326A/K328A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey physical association physical association false false false