DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ hierro-2007-1 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Hierro A., Rojas AL., Rojas R., Murthy N., Effantin G., Kajava AV., Steven AC., Bonifacino JS., Hurley JH. DIP original experiment: DIP-253030X Nature 2007 IMEx Functional architecture of the retromer cargo-recognition complex. Hierro A., Rojas AL., Rojas R., Murthy N., Effantin G., Kajava AV., Steven AC., Bonifacino JS., Hurley JH. Only protein-protein interactions imex curation hierro-2007-2 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Hierro A., Rojas AL., Rojas R., Murthy N., Effantin G., Kajava AV., Steven AC., Bonifacino JS., Hurley JH. DIP original experiment: DIP-253034X Nature 2007 IMEx Functional architecture of the retromer cargo-recognition complex. Hierro A., Rojas AL., Rojas R., Murthy N., Effantin G., Kajava AV., Steven AC., Bonifacino JS., Hurley JH. Only protein-protein interactions imex curation hierro-2007-3 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Hierro A., Rojas AL., Rojas R., Murthy N., Effantin G., Kajava AV., Steven AC., Bonifacino JS., Hurley JH. DIP original experiment: DIP-253037X Nature 2007 IMEx Functional architecture of the retromer cargo-recognition complex. Hierro A., Rojas AL., Rojas R., Murthy N., Effantin G., Kajava AV., Steven AC., Bonifacino JS., Hurley JH. Only protein-protein interactions imex curation hierro-2007-4 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Hierro A., Rojas AL., Rojas R., Murthy N., Effantin G., Kajava AV., Steven AC., Bonifacino JS., Hurley JH. DIP original experiment: DIP-253035X Nature 2007 IMEx Functional architecture of the retromer cargo-recognition complex. Hierro A., Rojas AL., Rojas R., Murthy N., Effantin G., Kajava AV., Steven AC., Bonifacino JS., Hurley JH. Only protein-protein interactions imex curation hierro-2007-5 ecolx Escherichia coli pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Hierro A., Rojas AL., Rojas R., Murthy N., Effantin G., Kajava AV., Steven AC., Bonifacino JS., Hurley JH. DIP original experiment: DIP-253033X Nature 2007 IMEx Functional architecture of the retromer cargo-recognition complex. Hierro A., Rojas AL., Rojas R., Murthy N., Effantin G., Kajava AV., Steven AC., Bonifacino JS., Hurley JH. Only protein-protein interactions imex curation hierro-2007-6 in vitro In vitro tem transmission electron microscopy tem predetermined predetermined participant predetermined Hierro A., Rojas AL., Rojas R., Murthy N., Effantin G., Kajava AV., Steven AC., Bonifacino JS., Hurley JH. DIP original experiment: DIP-253038X Nature 2007 IMEx Functional architecture of the retromer cargo-recognition complex. Hierro A., Rojas AL., Rojas R., Murthy N., Effantin G., Kajava AV., Steven AC., Bonifacino JS., Hurley JH. Only protein-protein interactions imex curation hierro-2007-7 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Hierro A., Rojas AL., Rojas R., Murthy N., Effantin G., Kajava AV., Steven AC., Bonifacino JS., Hurley JH. DIP original experiment: DIP-253031X Nature 2007 IMEx Functional architecture of the retromer cargo-recognition complex. Hierro A., Rojas AL., Rojas R., Murthy N., Effantin G., Kajava AV., Steven AC., Bonifacino JS., Hurley JH. Only protein-protein interactions imex curation hierro-2007-8 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Hierro A., Rojas AL., Rojas R., Murthy N., Effantin G., Kajava AV., Steven AC., Bonifacino JS., Hurley JH. DIP original experiment: DIP-253036X Nature 2007 IMEx Functional architecture of the retromer cargo-recognition complex. Hierro A., Rojas AL., Rojas R., Murthy N., Effantin G., Kajava AV., Steven AC., Bonifacino JS., Hurley JH. Only protein-protein interactions imex curation hierro-2007-9 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Hierro A., Rojas AL., Rojas R., Murthy N., Effantin G., Kajava AV., Steven AC., Bonifacino JS., Hurley JH. DIP original experiment: DIP-253032X Nature 2007 IMEx Functional architecture of the retromer cargo-recognition complex. Hierro A., Rojas AL., Rojas R., Murthy N., Effantin G., Kajava AV., Steven AC., Bonifacino JS., Hurley JH. Only protein-protein interactions imex curation vps35_human Vacuolar protein sorting-associated protein 35 Vesicle protein sorting 35 Maternal-embryonic 3 VPS35 MEM3 TCCCTA00141 protein protein human Homo sapiens Human MPTTQQSPQDEQEKLLDEAIQAVKVQSFQMKRCLDKNKLMDALKHASNMLGELRTSMLSPKSYYELYMAISDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVGVVYVKSFPQSRKDILKDLVEMCRGVQHPLRGLFLRNYLLQCTRNILPDEGEPTDEETTGDISDSMDFVLLNFAEMNKLWVRMQHQGHSRDREKRERERQELRILVGTNLVRLSQLEGVNVERYKQIVLTGILEQVVNCRDALAQEYLMECIIQVFPDEFHLQTLNPFLRACAELHQNVNVKNIIIALIDRLALFAHREDGPGIPADIKLFDIFSQQVATVIQSRQDMPSEDVVSLQVSLINLAMKCYPDRVDYVDKVLETTVEIFNKLNLEHIATSSAVSKELTRLLKIPVDTYNNILTVLKLKHFHPLFEYFDYESRKSMSCYVLSNVLDYNTEIVSQDQVDSIMNLVSTLIQDQPDQPVEDPDPEDFADEQSLVGRFIHLLRSEDPDQQYLILNTARKHFGAGGNQRIRFTLPPLVFAAYQLAFRYKENSKVDDKWEKKCQKIFSFAHQTISALIKAELAELPLRLFLQGALAAGEIGFENHETVAYEFMSQAFSLYEDEISDSKAQLAAITLIIGTFERMKCFSEENHEPLRTQCALAASKLLKKPDQGRAVSTCAHLFWSGRNTDKNGEELHGGKRVMECLKKALKIANQCMDPSLQVQLFIEILNRYIYFYEKENDAVTIQVLNQLIQKIREDLPNLESSEETEQINKHFHNTLEHLRLRRESPESEGPIYEGLIL vps29_human Vacuolar protein sorting-associated protein 29 Vesicle protein sorting 29 PEP11 homolog VPS29 DC7 DC15 MDS007 protein protein human Homo sapiens Human MLVLVLGDLHIPHRCNSLPAKFKKLLVPGKIQHILCTGNLCTKESYDYLKTLAGDVHIVRGDFDENLNYPEQKVVTVGQFKIGLIHGHQVIPWGDMASLALLQRQFDVDILISGHTHKFEAFEHENKFYINPGSATGAYNALETNIIPSFVLMDIQASTVVTYVYQLIGDDVKVERIEYKKP vp26a_human Vacuolar protein sorting-associated protein 26A VPS26A VPS26 Vesicle protein sorting 26A protein protein human Homo sapiens Human MSFLGGFFGPICEIDIVLNDGETRKMAEMKTEDGKVEKHYLFYDGESVSGKVNLAFKQPGKRLEHQGIRIEFVGQIELFNDKSNTHEFVNLVKELALPGELTQSRSYDFEFMQVEKPYESYIGANVRLRYFLKVTIVRRLTDLVKEYDLIVHQLATYPDVNNSIKMEVGIEDCLHIEFEYNKSKYHLKDVIVGKIYFLLVRIKIQHMELQLIKKEITGIGPSTTTETETIAKYEIMDGAPVKGESIPIRLFLAGYDPTPTMRDVNKKFSVRYFLNLVLVDEEDRRYFKQQEIILWRKAPEKLRKQRTNFHQRFESPESQASAEQPEM vps29-vps35-10 3161585 N/A 3161594 unspecified role unspecified role unspecified role unspecified role Region 476-780 Region 476-780 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3161595 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false vps29-vps26a-4 3161586 N/A 3161596 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3161594 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3161595 unspecified role unspecified role bait bait Q9UBQ0:p.Pro92Gly P92G mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q9UBQ0:p.Trp93Asp W93D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q9UBQ0:p.Gly94Pro G94P mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q9UBQ0:p.Arg104Asp R104D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q9UBQ0:p.[Tyr139Ala;Ala141Asp;Leu142Asp] Y139A/A141D/L142D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Q9UBQ0:p.Pro92Ala P92A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q9UBQ0:p.Trp93Ala W93A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human association association false false false vps35-vps29-10 3161587 N/A 3161595 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3161594 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q96QK1:p.[His769Ala;Asn772Ala;His776Ala] H769A/N772A/H776A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q96QK1:p.[Asn725Ala;Tyr729Ala] N725A/Y729A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q96QK1:p.[Thr672Asp;His675Asp] T672D/H675D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q96QK1:p.[Thr629Asp;Leu630Ala;Gly633Asp;Arg637Asp] T629D/L630A/G633D/R637D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q96QK1:p.[Leu579Asp;Arg582Asp;Gln586Ala;Leu589Asp] L579D/R582D/Q586A/Q589D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q96QK1:p.[Phe534Asp;Phe541Asp] F534D/F541D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human physical association physical association false false false vps35-vps26a-9 3161588 N/A 3161596 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3161595 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3161594 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human association association false false false vps35-vps26a-10 3161589 N/A 3161596 unspecified role unspecified role prey prey Region 162-327 Region 162-327 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3161594 unspecified role unspecified role bait bait Region 1-165 Region 1-165 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false vps26a-vps29-4 3161590 N/A 3161596 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3161594 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3161595 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false vps29-vps35-11 3161591 N/A 3161594 unspecified role unspecified role unspecified role unspecified role Region 476-780 Region 476-780 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3161595 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false vps35-vps26a-11 3161592 N/A 3161596 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3161594 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q96QK1:p.[Phe534Asp;Phe541Asp] F534D/F541D mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q96QK1:p.[Leu579Asp;Arg582Asp;Gln586Ala;Leu589Asp] L579D/R582D/Q586A/Q589D mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q96QK1:p.[Thr629Asp;Leu630Ala;Gly633Asp;Arg637Asp] T629D/L630A/G633D/R637D mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q96QK1:p.[Thr672Asp;His675Asp] T672D/H675D mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q96QK1:p.[Asn725Ala;Tyr729Ala] N725A/Y729A mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q96QK1:p.[His769Ala;Asn772Ala;His776Ala] H769A/N772A/H776A mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human physical association physical association false false false vps26a-vps29-5 3161593 N/A 3161596 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3161594 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3161595 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false