DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ bennett-2007-1 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Bennett EJ., Shaler TA., Woodman B., Ryu KY., Zaitseva TS., Becker CH., Bates GP., Schulman H., Kopito RR. DIP original experiment: DIP-253153X Nature 2007 IMEx Global changes to the ubiquitin system in Huntington's disease. Bennett EJ., Shaler TA., Woodman B., Ryu KY., Zaitseva TS., Becker CH., Bates GP., Schulman H., Kopito RR. Only protein-protein interactions imex curation bennett-2007-2 in vitro In vitro pull down pull down fingerprinting peptide massfingerprinting fingerprinting Bennett EJ., Shaler TA., Woodman B., Ryu KY., Zaitseva TS., Becker CH., Bates GP., Schulman H., Kopito RR. DIP original experiment: DIP-253154X Nature 2007 IMEx Global changes to the ubiquitin system in Huntington's disease. Bennett EJ., Shaler TA., Woodman B., Ryu KY., Zaitseva TS., Becker CH., Bates GP., Schulman H., Kopito RR. Only protein-protein interactions imex curation ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG ubql2_human Ubiquilin-2 UBQLN2 PLIC2 HRIHFB2157 N4BP4 Protein linking IAP with cytoskeleton 2 Ubiquitin-like product Chap1/Dsk2 DSK2 homolog Chap1 protein protein human Homo sapiens Human MAENGESSGPPRPSRGPAAAQGSAAAPAEPKIIKVTVKTPKEKEEFAVPENSSVQQFKEAISKRFKSQTDQLVLIFAGKILKDQDTLIQHGIHDGLTVHLVIKSQNRPQGQSTQPSNAAGTNTTSASTPRSNSTPISTNSNPFGLGSLGGLAGLSSLGLSSTNFSELQSQMQQQLMASPEMMIQIMENPFVQSMLSNPDLMRQLIMANPQMQQLIQRNPEISHLLNNPDIMRQTLEIARNPAMMQEMMRNQDLALSNLESIPGGYNALRRMYTDIQEPMLNAAQEQFGGNPFASVGSSSSSGEGTQPSRTENRDPLPNPWAPPPATQSSATTSTTTSTGSGSGNSSSNATGNTVAAANYVASIFSTPGMQSLLQQITENPQLIQNMLSAPYMRSMMQSLSQNPDLAAQMMLNSPLFTANPQLQEQMRPQLPAFLQQMQNPDTLSAMSNPRAMQALMQIQQGLQTLATEAPGLIPSFTPGVGVGVLGTAIGPVGPVTPIGPIGPIVPFTPIGPIGPIGPTGPAAPPGSTGSGGPTGPTVSSAAPSETTSPTSESGPNQQFIQQMVQALAGANAPQLPNPEVRFQQQLEQLNAMGFLNREANLQALIATGGDINAAIERLLGSQPS ubqln2-ub-1 3159451 N/A 3159453 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3159454 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false UBA domain Region 575-624 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false ubqln2-ub-2 3159452 N/A 3159453 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3159454 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false UBA domain Region 575-624 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false